Vector Name:
pJFT7_nHalo_DC(r3)
Synonyms:
''
Sequencing Primer:
Forward:
HaloseqF
Reverse:
pCPD6268_R
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6229
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Justin Saul
Ji Qiu
Fernanda Festa
Center for Personalized Diagnostics
Joshua LaBaer
Justin Saul
Ji Qiu
Fernanda Festa
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 bacterial origin
3514
4196
death cassette
ccdB (BsrGI removed)
ccdB death cassette - removed in plasmids containing a gene insert
2692
2997
enhancer
CITE
CITE enhancer
17
515
primer
forward primer
Forward sequencing primer HaloseqF aagcctgcctaactgcaa
1289
1360
primer
reverse primer
Reverse sequencing primer pCPD6268_R agccaactcagcttccttt
3206
3224
promoter
T7
T7 promoter
6205
6229
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1482
recombination site
attR2
attR2 recombination site
3138
3162
reporter gene
LacZ
LacZ alpha
6050
6118
selectable marker
AmpR
ampicillin resistance
4294
4953
selectable marker
CmR
chloramphenicol resistance - removed in plasmids containing a gene insert
1691
2347
ssDNA origin
f1
F1 ssDNA origin
5722
6028
tag
HALO
N-terminal TEV-cleavable Halo tag
519
1409
trxn termination sequence
T7 term
T7 transcription termination
3285
3332
