Vector Name:
pRSET-natTEVGFPHis
Synonyms:
''
Description:
Bacterial expression vector with T7 promoter and C-terminal TEV-cleavavable GFP and 6xHis-tags; ampicillin resistance in bacteria; ligation-independent (e.g., Clontech InFusion) following digestion with BseRI
Comments:
ligation-independent (e.g., Clontech InFusion) following digestion with BseRI
Size (bp):
4016
Parent Vector:
None
Properties:
bacterial expression, fluorescent marker, ligation independent cloning (LIC), with tag/fusion/marker
Author Name:
Arizona State University
Felicia M. Craciunescu
Center for Membrane Proteins in Infectious Diseases
PSI:Biology
Felicia M. Craciunescu
Center for Membrane Proteins in Infectious Diseases
PSI:Biology
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3166
3839
promoter
T7
T7 promoter
20
39
protease cleavage site
TEV
TEV protease cleavage site
532
549
ribosome binding site
RBS
ribosome binding site
85
92
selectable marker
AmpR
ampicillin resistance
2161
3021
ssDNA origin
f1
f1 origin
1575
2030
tag
GFP
C-terminal GFP
586
1299
tag
His
C-terminal 6xHis tag
1303
1320
trxn termination sequence
T7 term
T7 terminator
1375
1504
