Vector Name:
pCMVi-LSrCOMPTT
Synonyms:
''
Description:
with tag/fusion/marker assay, ligation independent cloning (LIC) clone, mammalian expression expression with intron and CMV and oligomerization domain and hGH and SV40 and leader sequence and AmpR and f1 and ColE and peptide and -10/Spec-R and COMP and Tetanus Toxoid and epitope and Stop Codons and InTron For and 013_DO3_DTH21 and RBS and 014_C03_DTH22 and 2012 and HGHTER and CMV-F; ampicillin resistance in bacterial;
Comments:
Clone insert in-frame using the BglII restriction sites and another downstream site before the transcription terminator (such as XmaI)
Size (bp):
5476
Parent Vector:
None
Properties:
ligation independent cloning (LIC), mammalian expression, with tag/fusion/marker
Author Name:
Arizona State University
Center for Membrane Proteins in Infectious Diseases
PSI:Biology
Center for Membrane Proteins in Infectious Diseases
PSI:Biology
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
-10 signal
-10/Spec-R
0
0
3' Clip
Stop Codons
1625
1630
3' Clip
013_DO3_DTH21
774
1615
3' Clip
014_C03_DTH22
735
1631
CDS
COMP
1244
1378
Origin
SV40
2316
2622
bacterial origin
ColE
ColE1 origin
2982
3839
binding site
RBS
1070
1082
domain
oligomerization domain
N-terminal pentamerizing COMP domain from rat matrix protein
1223
1378
intron
intron
intron
861
993
leader sequence
leader sequence
secretion leader sequence from human alpha1-antitrypsin
1079
1150
peptide
Tetanus Toxoid
P30
1406
1450
peptide
peptide
N-terminal highly immunogenic 20-mer random peptide
1160
1219
peptide
epitope
N-terminal tetanus toxin epitopes to overcome humoral tolerance
1478
1546
primer
InTron For
976
994
primer
2012
1679
1698
primer
HGHTER
1678
1698
primer
CMV-F
672
692
promoter
CMV
CMV promoter
1
744
selectable marker
AmpR
ampicillin resistance
3849
4758
ssDNA origin
f1
f1 origin
4841
5285
trxn termination sequence
hGH
hGH terminator
1640
2116
