Vector for antibody production in mice upon genetic immunization by gene gun with CMV promoter and N-terminal mouse ubiqiutin protein with Gly76Ala mutation; ampicillin resistance in bacteria; restriction enzyme cloning
Comments:
The mouse ubiquitin sequence contains the C-terminal Gly76Ala mutation to prevent de-ubiquination. Clone insert in-frame using the BglII restriction sites and another downstream site before the transcription terminator (such as XmaI)
Size (bp):
5218
Parent Vector:
None
Properties:
mammalian expression, multiple cloning site, with tag/fusion/marker
Author Name:
Arizona State University
Stephen Johnston
Kathryn Sykes