Detailed Vector Information: pRSET-YtfM-AN

Vector Name:
pRSET-YtfM-AN
Synonyms:
''
Sequencing Primer:
Forward:  T7
Reverse:  T7 terminator
Description:
Bacterial expression vector with T7 promoter and N-terminal E. coli YtfM (aa1-23) and 7xHis-tag; ampicillin resistance in bacteria; ligation-independent (e.g., Clontech InFusion) following digestion with BseRI
Comments:
Signal sequence is aa 1-23 from E. coli YtfM, which was supposedly used in PMID 20060837. Unfortunately, the actual sequence used in this publication was actually YtfM aa 1-27 with the V20A mutation, followed by 6xHis + LE (van den Berg, personal communication)
Size (bp):
3314
Parent Vector:
None
Properties:
bacterial expression, ligation independent cloning (LIC), with tag/fusion/marker
Author Name:
Felicia M. Craciunescu
Center for Membrane Proteins in Infectious Diseases
PSI:Biology
Publications:
None
Vector Map:
Vector Sequence:
Sequence:

Vector Features:

Type
Name
Description
Start
End
promoter
T7
T7 promoter
20
39
ribosome binding site
RBS
Ribosome binding site
85
92
selectable marker
AmpR
ampicillin resistance
1459
2319
signal sequence
YtfM
N-terminal E. coli YtfM (aa1-23) signal sequence
100
168
ssDNA origin
f1
f1 origin
873
1328
tag
His
N-terminal 7xHis tag
169
189
trxn termination sequence
T7 term
T7 termination
367
802