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IFNA21 (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  IFNA21
Gene Name:  interferon, alpha 21
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH116450.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: PMID: 16512675
Title: Functional proteomics approach to investigate the biological activities of cDNAs implicated in breast cancer.
Authors: HIP
HsCD00000010

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 570nts         Open reading frame : 1 to 570
Reference Sequence Alignment

ATGGCCCTGTCCTTTTCTTTACTGATGGCCGTGCTGGTGCTCAGCTACAAATCCATCTGT
TCTCTGGGCTGTGATCTGCCTCAGACCCACAGCCTGGGTAATAGGAGGGCCTTGATACTC
CTGGCACAAATGGGAAGAATCTCTCCTTTCTCCTGCCTGAAGGACAGACATGACTTTGGA
TTCCCCCAGGAGGAGTTTGATGGCAACCAGTTCCAGAAGGCTCAAGCCATCTCTGTCCTC
CATGAGATGATCCAGCAGACCTTCAATCTCTTCAGCACAAAGGACTCATCTGCTACTTGG
GAACAGAGCCTCCTAGAAAAATTTTCCACTGAACTTAACCAGCAGCTGAATGACCTGGAA
GCCTGCGTGATACAGGAGGTTGGGGTGGAAGAGACTCCCCTGATGAATGTGGACTCCATC
CTGGCTGTGAAGAAATACTTCCAAAGAATCACTCTTTATCTGACAGAGAAGAAATACAGC
CCTTGTGCCTGGGAGGTTGTCAGAGCAGAAATCATGAGATCCTTCTCTTTATCAAAAATT
TTTCAAGAAAGATTAAGGAGGAAGGAATAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 618
Start on reference sequence 49
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 1015 1048
recombination site loxP site loxP recombination site 9 42
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : IFNA21
Symbol Nomenclature : IFNA21
SYNONYM : IFN-alphaI; LeIF F; leIF-F
Designation : IFN-alpha-21|interferon alpha-21|interferon alpha-F|leukocyte interferon protein
Full Nomenclature : interferon, alpha 21
GENEID : 3452
GI : 61354766
GenBank Accession : AY888109
HGNC : 5424
MIM : 147584
Vega : OTTHUMG00000019653
Target GenBank: X00145

 

Reference Sequence Alignment

  

HsCD00000010      ATGGCCCTGTCCTTTTCTTTACTGATGGCCGTGCTGGTGCTCAGCTACAAATCCATCTGT
NM_002175.2       ATGGCCCTGTCCTTTTCTTTACTGATGGCCGTGCTGGTGCTCAGCTACAAATCCATCTGT
NM_021068.2       ATGGCCCTGTCCTTTTCTTTACTGATGGCCGTGCTGGTGCTCAGCTACAAATCCATCTGT
NM_002171.2       ATGGCCCTGTCCTTTTCTTTACTTATGGCCGTGCTGGTGCTCAGCTACAAATCCATCTGT
NM_021268.2       ATGGCCCTGTCCTTTTCTTTACTGATGGCCGTGCTGGTGCTCAGCTACAAATCCATCTGT
                  *********************** ************************************

HsCD00000010      TCTCTGGGCTGTGATCTGCCTCAGACCCACAGCCTGGGTAATAGGAGGGCCTTGATACTC
NM_002175.2       TCTCTGGGCTGTGATCTGCCTCAGACCCACAGCCTGGGTAATAGGAGGGCCTTGATACTC
NM_021068.2       TCTCTGGGCTGTGATCTGCCTCAGACCCACAGCCTGGGTAATAGGAGGGCCTTGATACTC
NM_002171.2       TCTCTAGGCTGTGATCTGCCTCAGACCCACAGCCTGGGTAATAGGAGGGCCTTGATACTC
NM_021268.2       TCTCTAGGCTGTGATCTGCCTCAGACCCACAGCCTGGGTAATAGGAGGGCCTTGATACTC
                  *****.******************************************************

HsCD00000010      CTGGCACAAATGGGAAGAATCTCTCCTTTCTCCTGCCTGAAGGACAGACATGACTTTGGA
NM_002175.2       CTGGCACAAATGGGAAGAATCTCTCCTTTCTCCTGCCTGAAGGACAGACATGACTTTGGA
NM_021068.2       CTGGCACAAATGGGAAGAATCTCTCATTTCTCCTGCCTGAAGGACAGACATGATTTCGGA
NM_002171.2       CTGGGACAAATGGGAAGAATCTCTCCTTTCTCCTGCCTGAAGGACAGACATGATTTCCGA
NM_021268.2       CTGGCACAAATGGGAAGAATCTCTCCTTTCTCCTGCCTGAAGGACAGACATGACTTTGGA
                  **** ********************.*************************** **  **

HsCD00000010      TTCCCCCAGGAGGAGTTTGATGGCAACCAGTTCCAGAAGGCTCAAGCCATCTCTGTCCTC
NM_002175.2       TTCCCCCAGGAGGAGTTTGATGGCAACCAGTTCCAGAAGGCTCAAGCCATCTCTGTCCTC
NM_021068.2       TTCCCCGAGGAGGAGTTTGATGGCCACCAGTTCCAGAAGGCTCAAGCCATCTCTGTCCTC
NM_002171.2       ATCCCCCAGGAGGAGTTTGATGGCAACCAGTTCCAGAAGGCTCAAGCCATCTCTGTCCTC
NM_021268.2       CTTCCCCAGGAGGAGTTTGATGGCAACCAGTTCCAGAAGACTCAAGCCATCTCTGTCCTC
                   * *** *****************.**************.********************

HsCD00000010      CATGAGATGATCCAGCAGACCTTCAATCTCTTCAGCACAAAGGACTCATCTGCTACTTGG
NM_002175.2       CATGAGATGATCCAGCAGACCTTCAATCTCTTCAGCACAAAGGACTCATCTGCTACTTGG
NM_021068.2       CATGAGATGATCCAGCAGACCTTCAATCTCTTCAGCACAGAGGACTCATCTGCTGCTTGG
NM_002171.2       CATGAGATGATCCAGCAGACCTTCAATCTCTTCAGCACAGAGGACTCATCTGCTGCTTGG
NM_021268.2       CATGAGATGATCCAGCAGACCTTCAATCTCTTCAGCACAGAGGACTCATCTGCTGCTTGG
                  ***************************************.**************.*****

HsCD00000010      GAACAGAGCCTCCTAGAAAAATTTTCCACTGAACTTAACCAGCAGCTGAATGACCTGGAA
NM_002175.2       GAACAGAGCCTCCTAGAAAAATTTTCCACTGAACTTAACCAGCAGCTGAATGACCTGGAA
NM_021068.2       GAACAGAGCCTCCTAGAAAAATTTTCCACTGAACTTTACCAGCAACTGAATGACCTGGAA
NM_002171.2       GAACAGAGCCTCCTAGAAAAATTTTCCACTGAACTTTACCAGCAACTGAATGACCTGGAA
NM_021268.2       GAACAGAGCCTCCTAGAAAAATTTTCCACTGAACTTTACCAGCAACTGAATAACCTGGAA
                  ************************************:*******.******.********

HsCD00000010      GCCTGCGTGATACAGGAGGTTGGGGTGGAAGAGACTCCCCTGATGAATGTGGACTCCATC
NM_002175.2       GCCTGCGTGATACAGGAGGTTGGGGTGGAAGAGACTCCCCTGATGAATGTGGACTCCATC
NM_021068.2       GCATGTGTGATACAGGAGGTTGGGGTGGAAGAGACTCCCCTGATGAATGAGGACTCCATC
NM_002171.2       GCATGTGTGATACAGGAGGTTGGGGTGGAAGAGACTCCCCTGATGAATGAGGACTCCATC
NM_021268.2       GCATGTGTGATACAGGAGGTTGGGATGGAAGAGACTCCCCTGATGAATGAGGACTCCATC
                  **.** ******************.************************:**********

HsCD00000010      CTGGCTGTGAAGAAATACTTCCAAAGAATCACTCTTTATCTGACAGAGAAGAAATACAGC
NM_002175.2       CTGGCTGTGAAGAAATACTTCCAAAGAATCACTCTTTATCTGACAGAGAAGAAATACAGC
NM_021068.2       CTGGCTGTGAGGAAATACTTCCAAAGAATCACTCTTTATCTAACAGAGAAGAAATACAGC
NM_002171.2       CTGGCTGTGAGGAAATACTTCCAAAGAATCACTCTTTATCTAATAGAGAGGAAATACAGC
NM_021268.2       CTGGCTGTGAGGAAATACTTCCAAAGAATCACTCTTTATCTAACAGAGAAGAAATACAGC
                  **********.******************************.* *****.**********

HsCD00000010      CCTTGTGCCTGGGAGGTTGTCAGAGCAGAAATCATGAGATCCTTCTCTTTATCAAAAATT
NM_002175.2       CCTTGTGCCTGGGAGGTTGTCAGAGCAGAAATCATGAGATCCTTCTCTTTATCAAAAATT
NM_021068.2       CCTTGTGCCTGGGAGGTTGTCAGAGCAGAAATCATGAGATCCCTCTCGTTTTCAACAAAC
NM_002171.2       CCTTGTGCCTGGGAGGTTGTCAGAGCAGAAATCATGAGATCCCTCTCGTTTTCAACAAAC
NM_021268.2       CCTTGTGCCTGGGAGGTTGTCAGAGCAGAAATCATGAGATCTCTCTCTTTTTCAACAAAC
                  *****************************************  **** **:****.**: 

HsCD00000010      TTTCAAGAAAGATTAAGGAGGAAGGAATAG
NM_002175.2       TTTCAAGAAAGATTAAGGAGGAAGGAATGA
NM_021068.2       TTGCAAAAAAGATTAAGGAGGAAGGATTGA
NM_002171.2       TTGCAAAAAAGATTAAGGAGGAAGGATTGA
NM_021268.2       TTGCAAAAAATATTAAGGAGGAAGGATTGA
                  ** ***.*** ***************:*..

Pathways

NCI : Downstream signaling in naïve CD8+ T cells
Reactome : Cytokine Signaling in Immune system
Reactome : Factors involved in megakaryocyte development and platelet production
Reactome : Hemostasis
Reactome : Immune System
Reactome : Innate Immune System
Reactome : Interferon Signaling
Reactome : Interferon alpha/beta signaling
Reactome : RIG-I/MDA5 mediated induction of IFN-alpha/beta pathways
Reactome : Regulation of IFNA signaling
Reactome : TRAF6 mediated IRF7 activation
WikiPathway : Regulation of toll-like receptor signaling pathway
WikiPathway : Toll-like receptor signaling pathway

Protein

SMART domain : IFabd : Interferon alpha, beta and delta.
UniProt : P01568
HPRD : 00968

Clone

Cloning Information : 116450
HIP Master Clone ID : 92296
Original Clone ID : FLH116450.01X

Organism


TAX_ID : 9606
Species Specific ID: 3452

Genome

Chromosome : 9
Map Location : 9p22
Ensembl : ENSG00000137080

Reagents

Labome : IFNA21-antibody