Sequencing Primers

DNASU uses various primers to sequence verify gene inserts for most of the plasmids avialable in the repository. For a full list of vectors and their sequencing primers, go to our Vector/Primer page.

If you are interested in Sanger or Next Generation sequencing Services, please refer to our Sequencing Core website for more information.

Common Sequencing Primers

Primer Name
Primer Sequence
AttP2
CAGGAAACAGCTATGAC
M13F
GTAAAACGACGGCCAGT
M13R
CAGGAAACAGCTATGACC
NAP138R
TGTTTCGCCATTTATCACCTTC
NAP150
CCCATTGTATGGGATCTGATC
T7
TAATACGACTCACTATAG
T7 terminator
GCTAGTTATTGCTCAGCGG

All Sequencing Primers

Primer Name
Primer Sequence
alt_bad_rev
GGGTCAGGTGGGACCAC
ANForward
CGTTTCCAGTCTCACCACC
AOX1
GACTGGTTCCAATTGACAAGC
ARAC5R
GGGATCATTTTGCGCTTCAG
AttP2
CAGGAAACAGCTATGAC
CAGF
TCGGCTTCTGGCGTGTGACC
CAGR
TGTCCCCATAATTTTTGGCAGAGG
cHaloseqR
CAACATCGACGTAGTGCAT
CMV forward
CGCAAATGGGCGGTAGGCGTG
DD122B
GTTAGGCGTTTTGCGCTG
DD123B
GCTGGCAACTAGAAGGCAC
DuetUp1
GGATCTCGACGCTCTCCCT
EGFP-C
CATGGTCCTGCTGGAGTTCGTG
EGFP-N
CGTCGCCGTCCAGCTCGACCAG
FasBacFwd
ATACCGTCCCACCATCG
FastBacRev
GATCCTCTAGTACTTCTCG
GFPR
TCTCCACTGACAGAAAATTTGTGC
GST Forward
CTTCGATAGGGTGTTTAACT
GSTseqF
GCAAGTATATAGCATGGCCT
HaloseqF
AAGCCTGCCTAACTGCAA
HGHTER
ACTGGAGTGGCAACTTCCAGG
His2 Forward
AGAAAGTTTATGAAGATCGATCTCAT
ISFI_newrev
TTTGTTAGCAGCCGGATCTCAGTGG
IST1_728
CGCCTTTCTCATATCCAC
JPO113
GCGGTTTTGGCAGTACATCAATGGGCG
LACI5R
CGTATAACGTTACTGGTTTCAC
LPP2
GATCAAAAATAAGTGGCGCCCCATC
LSFOR
TTCTGTCTCGTGGGGCAT
M13F
GTAAAACGACGGCCAGT
M13R
CAGGAAACAGCTATGACC
malE
GGTCGTCAGACTGTCGATGAAGCC
MBP Forward
CTACAGGCGAAAGACCATACG
MBPpelBseqF
GAAAGGTGAAATCATGCCGAACATC
MBPSeq Forward
TCGTCAGACTGTCGATGAAG
MCSG73F
TATGGCTGCCCGTAATAACAACAAC
nap1273r
TAAACGTTCAATTGCTATTATCACG
NAP138R
TGTTTCGCCATTTATCACCTTC
NAP150
CCCATTGTATGGGATCTGATC
None
p1
ATCG
P139
GAGGGCCTATTTCCCATGATTCCTT
p28mis forward
CGAACCGCTGATCCAACTGG
p28mis reverse
GATATAGTTCCTCCTTTCAGC
p4
GGTTTAAACGGATCCAGC
pant1491R
TTTTTGTTTAAACTACCACTTT
pant1627r
CCGGATATAGTTCCTCCTTT
pAOX1 Reverse
GCAAATGGCATTCTGACATCC
PB22F
GAGGAATTCACCATGCGCTATATC
PB22R
CAATAAGGAAAACTACTAAAAGAGCTTC
pBABE 3'
ACCCTAACTGACACACATTCC
pBABE 5'
CTTTATCCAGCCCTCAC
pBAD forward
ATGCCATAGCATTTTTATCC
PBADF
CGCAACTCTCTACTGTTTCTCC
PBADFOR
GCACGGCGTCACACTTTGC
PBADR
CCGCTTCTGCGTTCTGATTTAATCTG
pBADrev
TAGTTATTGCTCAGCGGTGG
pBRrevBam_Primer
GGTGATGTCGGCGATATAGG
pBRrevBqm_Primer
GGTGATGTCGGCGATATAGG
pCDNA F
TTAATACGACTCACTATAGGG
pcDNAF
AGAGAACCCACTGCTTACTGGCTTATC
pcDNAR
AACTAGAAGGCACAGTCGAGGCTG
pCPD5698_F
AATTCATGGACGAGAAGACC
pCPD6268_R
AGCCAACTCAGCTTCCTTT
pDONR201-forward
GTAACATCAGAGATTTTGAGACAC
pDONR201-reverse
TCGCGTTAACGCTAGCATGGATCTC
pET Upstream
ATGCGTCCGGCGTAGA
pEU Reverse
CCCGCTTCGCCGCCGCAGAAT
pEU319 Forward
GTCGTTAGAACGCGGCTAC
pEUfor
GGTGACACTATAGAACTCA
pEUrev
GGCGCTAGCCGTAAATTCT
pF1KseqR
CTTTCGGGCTTTGTTAGCAG
pFAB007
GGACGCATCGTGGCCC
pFB Forward
TATTCCGGATTATTCATACC
pFB Reverse
ACAAATGTGGTATGGCTGATT
pGEc1-DEST F
AGAGCTGGTTTAGTGAACCGTC
pGEn1-DEST R
TGACAACGGGCCACAACTCCTC
pGEn2-DEST F
GACAATCACTACCTGAGCACCA
pGEn3-DEST-5'F
AGCATGCACCACCACCATCACC
pGEX3
CCGGGAGCTGCATGTGTCAGAGG
pGEX5
GGGCTGGCAAGCCACGTTTGGTG
PGEXF
GGTGGTGGCGACCATCCTCC
PGEXR
GGTTTTCACCGTCATCACCG
Phsp
AGGTGACCACAACGACGC
pIEx Forward
AACCTCTACTTCCAATCGCACCATCATCACCACCATTG
pIEx Reverse
TATATCTCCTTCTTAAGGTTAAACAAAATTATTTCTAG
piex-seq-f
GCGACAAGATCGTGAACAAC
piex-seq-r
ACGGCCCCTCGATAATAAAA
pIEx_seq_F
GCGACAAGATCGTGAACAAC
pIEx_seq_R
ACGGCCCCTCGATAATAAAA
pJFT7-seqR
AGCGAGTCAGTGAGCGAGGA
pLIC rev
AGCAGCCAACTCAGCTTCC
pLICf
TGTGAGCGGATAACAATTCC
pMal
TGTCCTACTCAGGAGAGCGTTCAC
pMCSG48F
GAAGGGTTGACCGACGAAAAAGC
pMHTDelta238-F
ACGCCCAGAAAGGTGAAATCATGC
pMHTDelta238-R
TGAGTCGCTTCCTTAACTGGCTGA
pPIC3-5F
GACTGGTTCCAATTGACAAGC
pPIC3-5R
GCAAATGGCATTCTGACATCC
pQE Reverse
CAAGACTCCAGTAATGACC
PQEREV
GTTCTGAGGTCATTACTGG
Ps-Pimyc
CTGTTTAAACGGATCGTCG
pSGP-Forward
CAACATTTTCAGTTTGTATTACTTC
pSGP-Reverse
CAATCAACTATCAACTATTAACTA
pSGP18for
CACCTGTGCCGAAACGCAAATG
pSGP36 forward
CAACATTTTCAGTTTGTATTACTTC
pSGP36 reverse
CAAGGCGATTAAGTTGGGTAACGC
pSUPER-for
GGAAGCCTTGGCTTTTG
PtetO
GGATCGTCGGCACCGTCA
RRNBTER
TCTGCGTTCTGATTTAATCTGTATCA
snap_484F
CTCGCCGTGAAAGAGTGG
SP6
CATACGATTTAGGTGACACTATAG
T3
ATTAACCCTCACTAAAGGGA
T7
TAATACGACTCACTATAG
T7 EEV
AAGGCTAGAGTACTTAATACGA
T7 For
TAATACGACTCACTATAGGG
T7 short
TAATACGACTCACTATAGGG
T7 terminator
GCTAGTTATTGCTCAGCGG
T7p
AATTAATACGACTCACTATAGGG
T7short
TAATACGACTCACTATAGGG
T7t
ATGCTAGTTATTGCTCAGCGG
TEV
GAAAACCTGTACTTCCAG
TRXF
GTTGAAAGAGTTCCTCGACG
TTFOR
CAACAACTTCACCGTGAGC
U19
GTTTTCCCAGTCACGACGT
UBFOR
AACATCCAGAAGGAGTCA
V5 reverse
ACCGAGGAGAGGGTTAGGGAT
YFP
CATCAAGGTGAACTTCAAGA
ZsGfw
GGGCAAGGCGGAGCCGGAGGCGATGGCGTGCTCGGTCAGGTGCCACTTCTGGTTCTTGGCGTCGCTGCGGTCCTCGCGGGTCAGCTTGTGCTGGATGAAGTGC
ZsGrev
ATGGCCCAGTCCAAGCACGGCCTGACCAAGGAGATGACCATGAAGTACCGCATGGAGGGCTGCGTG