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Detailed Plasmid Information

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Explanation of Terms


Gene: Gene Symbol:  None
Gene Name:  None
Sequence             
Original Clone ID: 35475
Keyword: None
Species: Nostoc punctiforme PCC 73102
Type: cDNA
Vector Name: pSpeedET               Format:  CLOSED
Source: Joint Center for Structural Genomics
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: PSI
Joint Center for Structural Genomics
OoCD00104387

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AVAILABLE
No restriction
Special MTA: PSI

 

Insert sequence: 765nts         Open reading frame : 51 to 815

CTGATAAAATTCATCATCATCATCATCACGAAAACCTGTACTTCCAGGGCATGCTAACTG
CTGAACGACGACAATTCATCTTAGAAATTCTGCGTCGTGATAAAAAGGTACTTTCGACAG
AACTTAGTGCTGTTCTGAAGGTTTCTGAGGATACGATTCGCCGGGACTTGCGAGAACTGG
CAGAAACTGGACTATTGCAACGCGTTCATGGGGGAGCGCTCCTCAAATCTCCAGCCACGG
CCAGTTATGCCGATCGCCAAAAGCAAGCGCCAAAAGAAAAGGAAGCGATCGCTCGGACAG
CAGCAAAATTAGTTTGTACAGGACAGGTGGTGATTTTAGACGGAGGTACAACAACTCTCC
AAGTAACCCGTCATTTGCCCCTAGGTTTGCACGCAACAGTCATCACAAATAGTCCGCCAA
TCGCCGTTGCTTTAGCAGAACATCCCCATATAGAGGTTGTGATGTTAGGTGGACAACTTT
ACAAAAAAGCCTTGATGAACGTGGGTACTGTCACGATTGAGACTTTACGGATGATTCGTG
CAGATTTGTGCATGTTGGGAGTTTGCAGTTTACATCCAGAAGTTGGGATTAGTGTACCTA
ACCTAGACGAAGCCCACGTCAAACGAGCCATGATTGCTGGATCGGCTGAGGTAGTAGGAC
TAGTGACAGCAGAAAAATTAGACACGGCTGCTCCTTATCTAGTGGAGTCAATTCATGCCC
TGACTTACCTAGTAACAGCACCTACAGTATCTAATAATATGTTGAGTGCCTACAAAGCTT
TAGGTTTGACCATTATACGTGATGAACATGAATAACGCGACTTAATTAA


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 765
Start on reference sequence 1
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
ATGGGATCTGATAAAATTCATCATCATCATCATCACGAAAACCTGTACTTCCAGGGC

3' Linker Sequence:
CGCGACTTAATTAAACGGTCTCCAGCTTGGCTGTTTTGGCGGATGAG

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: SolubleProtein: Tested_Not_Soluble
ProteinExpressed: Not_Applicable
ProteinPurified: Protein_Purified
Find experimental protocols in 379677
Recommended expression in: HK100
       DyNA Vector Map

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Vector Name: pSpeedET

pSpeedET in advanced viewed

Synonyms: pSpeed-ET
Sequencing Primer: Forward:  pBAD forward
Reverse:  alt_bad_rev
Description: Bacterial expression vector, arabinose or T7 induced expression, adds N-terminal MGSDKIHHHHHHENLYFQG tag; kanamycin resistance; PIPE cloning.
Comments: If this vector contains an insert, the ccdB death cassette will not be present.
Size (bp): 5464
Parent Vector: None
Empty Vector: EvNO00285791
Properties: PIPE cloning, bacterial expression, in vitro transcription, inducible expression, with tag/fusion/marker
Author Name: Joint Center for Structural Genomics
Publications: PMID: 18004753
Title: Combining the polymerase incomplete primer extension method for cloning and mutagenesis with microscreening to accelerate structural genomics efforts.

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori ColE1-type origin of replication 3306 3988
inducible trxn activator protein gene araC araC coding sequence 4563 5438
negative selection marker ccdB ccdB death cassette gene (lost in with-insert form) 1596 1805
promoter araC araC promoter 256 285
promoter T7 T7 promoter 290 310
protease cleavage site TEV TEV protease cleavage site 382 402
selectable marker CmR chloramphenicol resistance gene (CmR) (lost in with-insert form) 502 1158
selectable marker KanR kanamycin resistance gene 2396 3208
tag His N-terminal 6xHis tag 364 381
trxn regulatory element arabinose O2 operator arabinose O2 operator 5 23
trxn regulatory element arabinose O1 operator arabinose O1 operator 161 182
trxn termination sequence rrnB rrnB T2 transcription terminator 2258 2284

 

  • Protein
  • Clone
  • Experimental

Clone

Cloning Information : 308674
HIP Master Clone ID : 238160
Original Clone ID : 35475

Experimental

TargetTrack - Experimental data : 379677