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ERCC1 (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  ERCC1
Gene Name:  excision repair cross-complementing rodent repair deficiency, complementation group 1 (includes overlapping antisense sequence)
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH031193.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00000105

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 894nts        
Open reading frame : 1 to 894
Reference Sequence Alignment

ATGGACCCTGGGAAGGACAAAGAGGGGGTGCCCCAGCCCTCAGGGCCGCCAGCAAGGAAG
AAATTTGTGATACCCCTCGACGAGGATGAGGTCCCTCCTGGAGTGGCCAAGCCCTTATTC
CGATCTACACAGAGCCTTCCCACTGTGGACACCTCGGCCCAGGCGGCCCCTCAGACCTAC
GCCGAATATGCCATCTCACAGCCTCTGGAAGGGGCTGGGGCCACGTGCCCCACAGGGTCA
GAGCCCCTGGCAGGAGAGACGCCCAACCAGGCCCTGAAACCCGGGGCAAAATCCAACAGC
ATCATTGTGAGCCCTCGGCAGAGGGGCAATCCCGTACTGAAGTTCGTGCGCAACGTGCCC
TGGGAATTTGGCGACGTAATTCCCGACTATGTGCTGGGCCAGAGCACCTGTGCCCTGTTC
CTCAGCCTCCGCTACCACAACCTGCACCCAGACTACATCCATGGGCGGCTGCAGAGCCTG
GGGAAGAACTTCGCCTTGCGGGTCCTGCTTGTCCAGGTGGATGTGAAAGATCCCCAGCAG
GCCCTCAAGGAGCTGGCTAAGATGTGTATCCTGGCCGACTGCACATTGATCCTCGCCTGG
AGCCCCGAGGAAGCTGGGCGGTACCTGGAGACCTACAAGGCCTATGAGCAGAAACCAGCG
GACCTCCTGATGGAGAAGCTAGAGCAGGACTTCGTCTCCCGGGTGACTGAATGTCTGACC
ACCGTGAAGTCAGTCAACAAAACGGACAGTCAGACCCTCCTGACCACATTTGGATCTCTG
GAACAGCTCATCGCCGCATCAAGAGAAGATCTGGCCTTATGCCCAGGCCTGGGCCCTCAG
AAAGCCCGGAGGCTGTTTGATGTCCTGCACGAGCCCTTCTTGAAAGTACCCTGA


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1036
Start on reference sequence 143
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: ''
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 9 42
recombination site loxP site loxP recombination site 1015 1048
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ERCC1
Symbol Nomenclature : ERCC1
SYNONYM : COFS4; RAD10; UV20
Designation : DNA excision repair protein ERCC-1
Full Nomenclature : excision repair cross-complementing rodent repair deficiency, complementation group 1 (includes overlapping antisense sequence)
GENEID : 2067
GI : 61354942
GenBank Accession : AY888132
HGNC : 3433
MIM : 126380
Vega : OTTHUMG00000182121
Target GenBank: NM_001983

 

Reference Sequence Alignment

  

HsCD00000105        ATGGACCCTGGGAAGGACAAAGAGGGGGTGCCCCAGCCCTCAGGGCCGCCAGCAAGGAAG
NM_001166049.1      ATGGACCCTGGGAAGGACAAAGAGGGGGTGCCCCAGCCCTCAGGGCCGCCAGCAAGGAAG
NM_202001.2         ATGGACCCTGGGAAGGACAAAGAGGGGGTGCCCCAGCCCTCAGGGCCGCCAGCAAGGAAG
XM_005258634.1      ATGGACCCTGGGAAGGACAAAGAGGGGGTGCCCCAGCCCTCAGGGCCGCCAGCAAGGAAG
XM_005258637.1      ATGGACCCTGGGAAGGACAAAGAGGGGGTGCCCCAGCCCTCAGGGCCGCCAGCAAGGAAG
NM_001983.3         ATGGACCCTGGGAAGGACAAAGAGGGGGTGCCCCAGCCCTCAGGGCCGCCAGCAAGGAAG
XM_005258635.1      ATGGACCCTGGGAAGGACAAAGAGGGGGTGCCCCAGCCCTCAGGGCCGCCAGCAAGGAAG
                    ************************************************************

HsCD00000105        AAATTTGTGATACCCCTCGACGAGGATGAGGTCCCTCCTGGAGTGGCCAAGCCCTTATTC
NM_001166049.1      AAATTTGTGATACCCCTCGACGAGGATGAGGTCCCTCCTGGAGTGGCCAAGCCCTTATTC
NM_202001.2         AAATTTGTGATACCCCTCGACGAGGATGAGGTCCCTCCTGGAGTGGCCAAGCCCTTATTC
XM_005258634.1      AAATTTGTGATACCCCTCGACGAGGATGAGGTCCCTCCTGGAGTGGCCAAGCCCTTATTC
XM_005258637.1      AAATTTGTGATACCCCTCGACGAGGATGAGGTCCCTCCTGGAGTGGCCAAGCCCTTATTC
NM_001983.3         AAATTTGTGATACCCCTCGACGAGGATGAGGTCCCTCCTGGAGTGGCCAAGCCCTTATTC
XM_005258635.1      AAATTTGTGATACCCCTCGACGAGGATGAGGTCCCTCCTGGAGTGGCCAAGCCCTTATTC
                    ************************************************************

HsCD00000105        CGATCTACACAGAGCCTTCCCACTGTGGACACCTCGGCCCAGGCGGCCCCTCAGACCTAC
NM_001166049.1      CGATCTACACAGAGCCTTCCCACTGTGGACACCTCGGCCCAGGCGGCCCCTCAGACCTAC
NM_202001.2         CGATCTACACAGAGCCTTCCCACTGTGGACACCTCGGCCCAGGCGGCCCCTCAGACCTAC
XM_005258634.1      CGATCTACACAGAGCCTTCCCACTGTGGACACCTCGGCCCAGGCGGCCCCTCAGACCTAC
XM_005258637.1      CGATCTACACAGAGCCTTCCCACTGTGGACACCTCGGCCCAGGCGGCCCCTCAGACCTAC
NM_001983.3         CGATCTACACAGAGCCTTCCCACTGTGGACACCTCGGCCCAGGCGGCCCCTCAGACCTAC
XM_005258635.1      CGATCTACACAGAGCCTTCCCACTGTGGACACCTCGGCCCAGGCGGCCCCTCAGACCTAC
                    ************************************************************

HsCD00000105        GCCGAATATGCCATCTCACAGCCTCTGGAAGGGGCTGGGGCCACGTGCCCCACAGGGTCA
NM_001166049.1      GCCGAATATGCCATCTCACAGCCTCTGGAAGGGGCTGGGGCCACGTGCCCCACAGGGTCA
NM_202001.2         GCCGAATATGCCATCTCACAGCCTCTGGAAGGGGCTGGGGCCACGTGCCCCACAGGGTCA
XM_005258634.1      GCCGAATATGCCATCTCACAGCCTCTGGAAGGGGCTGGGGCCACGTGCCCCACAGGGTCA
XM_005258637.1      GCCGAATATGCCATCTCACAGCCTCTGGAAGGGGCTGGGGCCACGTGCCCCACAGGGTCA
NM_001983.3         GCCGAATATGCCATCTCACAGCCTCTGGAAGGGGCTGGGGCCACGTGCCCCACAGGGTCA
XM_005258635.1      GCCGAATATGCCATCTCACAGCCTCTGGAAGGGGCTGGGGCCACGTGCCCCACAGGGTCA
                    ************************************************************

HsCD00000105        GAGCCCCTGGCAGGAGAGACGCCCAACCAGGCCCTGAAACCCGGGGCAAAATCCAACAGC
NM_001166049.1      GAGCCCCTGGCAGGAGAGACGCCCAACCAGGCCCTGAAACCCGGGGCAAAATCCAACAGC
NM_202001.2         GAGCCCCTGGCAGGAGAGACGCCCAACCAGGCCCTGAAACCCGGGGCAAAATCCAACAGC
XM_005258634.1      GAGCCCCTGGCAGGAGAGACGCCCAACCAGGCCCTGAAACCCGGGGCAAAATCCAACAGC
XM_005258637.1      GAGCCCCTGGCAGGAGAGACGCCCAACCAGGCCCTGAAACCCGGGGCAAAATCCAACAGC
NM_001983.3         GAGCCCCTGGCAGGAGAGACGCCCAACCAGGCCCTGAAACCCGGGGCAAAATCCAACAGC
XM_005258635.1      GAGCCCCTGGCAGGAGAGACGCCCAACCAGGCCCTGAAACCCGGGGCAAAATCCAACAGC
                    ************************************************************

HsCD00000105        ATCATTGTGAGCCCTCGGCAGAGGGGCAATCCCGTACTGAAGTTCGTGCGCAACGTGCCC
NM_001166049.1      ATCATTGTGAGCCCTCGGCAGAGGGGCAATCCCGTACTGAAGTTCGTGCGCAATGTGCCC
NM_202001.2         ATCATTGTGAGCCCTCGGCAGAGGGGCAATCCCGTACTGAAGTTCGTGCGCAATGTGCCC
XM_005258634.1      ATCATTGTGAGCCCTCGGCAGAGGGGCAATCCCGTACTGAAGTTCGTGCGCAATGTGCCC
XM_005258637.1      ATCATTGTGAGCCCTCGGCAGAGGGGCAATCCCGTACTGAAGTTCGTGCGCAATGTGCCC
NM_001983.3         ATCATTGTGAGCCCTCGGCAGAGGGGCAATCCCGTACTGAAGTTCGTGCGCAATGTGCCC
XM_005258635.1      ATCATTGTGAGCCCTCGGCAGAGGGGCAATCCCGTACTGAAGTTCGTGCGCAATGTGCCC
                    ***************************************************** ******

HsCD00000105        TGGGAATTTGGCGACGTAATTCCCGACTATGTGCTGGGCCAGAGCACCTGTGCCCTGTTC
NM_001166049.1      TGGGAATTTGGCGACGTAATTCCCGACTATGTGCTGGGCCAGAGCACCTGTGCCCTGTTC
NM_202001.2         TGGGAATTTGGCGACGTAATTCCCGACTATGTGCTGGGCCAGAGCACCTGTGCCCTGTTC
XM_005258634.1      TGGGAATTTGGCGACGTAATTCCCGACTATGTGCTGGGCCAGAGCACCTGTGCCCTGTTC
XM_005258637.1      TGGGAATTTGGCGACGTAATTCCCGACTATGTGCTGGGCCAGAGCACCTGTGCCCTGTTC
NM_001983.3         TGGGAATTTGGCGACGTAATTCCCGACTATGTGCTGGGCCAGAGCACCTGTGCCCTGTTC
XM_005258635.1      TGGGAATTTGGCGACGTAATTCCCGACTATGTGCTGGGCCAGAGCACCTGTGCCCTGTTC
                    ************************************************************

HsCD00000105        CTCAGCCTCCGCTACCACAACCTGCACCCAGACTACATCCATGGGCGGCTGCAGAGCCTG
NM_001166049.1      CTCAGCCTCCGCTACCACAACCTGCACCCAGACTACATCCATGGGCGGCTGCAGAGCCTG
NM_202001.2         CTCAGCCTCCGCTACCACAACCTGCACCCAGACTACATCCATGGGCGGCTGCAGAGCCTG
XM_005258634.1      CTCAGCCTCCGCTACCACAACCTGCACCCAGACTACATCCATGGGCGGCTGCAGAGCCTG
XM_005258637.1      CTCAGCCTCCGCTACCACAACCTGCACCCAGACTACATCCATGGGCGGCTGCAGAGCCTG
NM_001983.3         CTCAGCCTCCGCTACCACAACCTGCACCCAGACTACATCCATGGGCGGCTGCAGAGCCTG
XM_005258635.1      CTCAGCCTCCGCTACCACAACCTGCACCCAGACTACATCCATGGGCGGCTGCAGAGCCTG
                    ************************************************************

HsCD00000105        GGGAAGAACTTCGCCTTGCGGGTCCTGCTTGTCCAGGTGGATGTGAAAGATCCCCAGCAG
NM_001166049.1      GGGAAGAACTTCGCCTTGCGGGTCCTGCTTGTCCAGGTGGATGTGAAAGATCCCCAGCAG
NM_202001.2         GGGAAGAACTTCGCCTTGCGGGTCCTGCTTGTCCAGGTGGATGTGAAAGATCCCCAGCAG
XM_005258634.1      GGGAAGAACTTCGCCTTGCGGGTCCTGCTTGTCCAGGTGGATGTGAAAGATCCCCAGCAG
XM_005258637.1      GGGAAGAACTTCGCCTTGCGGGTCCTGCTTGTCCAGGTGGATGTGAAAGATCCCCAGCAG
NM_001983.3         GGGAAGAACTTCGCCTTGCGGGTCCTGCTTGTCCAGGTGGATGTGAAAGATCCCCAGCAG
XM_005258635.1      GGGAAGAACTTCGCCTTGCGGGTCCTGCTTGTCCAGGTGGATGTGAAAGATCCCCAGCAG
                    ************************************************************

HsCD00000105        GCCCTCAAGGAGCTGGCTAAGATGTGTATCCTGGCCGACTGCACATTGATCCTCGCCTGG
NM_001166049.1      GCCCTCAAGGAGCTGGCTAAGATGTGTATCCTGGCCGACTGCACATTGATCCTCGCCTGG
NM_202001.2         GCCCTCAAGGAGCTGGCTAAGATGTGTATCCTGGCCGACTGCACATTGATCCTCGCCTGG
XM_005258634.1      GCCCTCAAGGAGCTGGCTAAGATGTGTATCCTGGCCGACTGCACATTGATCCTCGCCTGG
XM_005258637.1      GCCCTCAAGGAGCTGGCTAAGATGTGTATCCTGGCCGACTGCACATTGATCCTCGCCTGG
NM_001983.3         GCCCTCAAGGAGCTGGCTAAGATGTGTATCCTGGCCGACTGCACATTGATCCTCGCCTGG
XM_005258635.1      GCCCTCAAGGAGCTGGCTAAGATGTGTATCCTGGCCGACTGCACATTGATCCTCGCCTGG
                    ************************************************************

HsCD00000105        AGCCCCGAGGAAGCTGGGCGGTACCTGGAGACCTACAAGGCCTATGAGCAGAAACCAGCG
NM_001166049.1      AGCCCCGAGGAAGCTGGGCGGTACCTGGAGACCTACAAGGCCTATGAGCAGAAACCAGCG
NM_202001.2         AGCCCCGAGGAAGCTGGGCGGTACCTGGAGACCTACAAGGCCTATGAGCAGAAACCAGCG
XM_005258634.1      AGCCCCGAGGAAGCTGGGCGGTACCTGGAGACCTACAAGGCCTATGAGCAGAAACCAGCG
XM_005258637.1      AGCCCCGAGGAAGCTGGGCGGTACCTGGAGACCTACAAGGCCTATGAGCAGAAACCAGCG
NM_001983.3         AGCCCCGAGGAAGCTGGGCGGTACCTGGAGACCTACAAGGCCTATGAGCAGAAACCAGCG
XM_005258635.1      AGCCCCGAGGAAGCTGGGCGGTACCTGGAGACCTACAAGGCCTATGAGCAGAAACCAGCG
                    ************************************************************

HsCD00000105        GACCTCCTGATGGAGAAGCTAGAGCAGGACTTCGTCTCCCGGG-----------------
NM_001166049.1      GACCTCCTGATGGAGAAGCTAGAGCAGGACTTCGTCTCCCGG------------------
NM_202001.2         GACCTCCTGATGGAGAAGCTAGAGCAGGACTTCGTCTCCCGGGTGACTGAATGTCTGACC
XM_005258634.1      GACCTCCTGATGGAGAAGCTAGAGCAGGACTTCGTCTCCCGGGTGACTGAATGTCTGACC
XM_005258637.1      GACCTCCTGATGGAGAAGCTAGAGCAGGACTTCGTCTCCCGG------------------
NM_001983.3         GACCTCCTGATGGAGAAGCTAGAGCAGGACTTCGTCTCCCGGG-----------------
XM_005258635.1      GACCTCCTGATGGAGAAGCTAGAGCAGGACTTCGTCTCCCGGGTGACTGAATGTCTGACC
                    ******************************************                  

HsCD00000105        ------------------------------------------------------------
NM_001166049.1      ------------------------------------------------------------
NM_202001.2         ACCGTGAAGTCAGTCAACAAAACGGACAGTCAGACCCTCCTGACCACATTTGGATCTCTG
XM_005258634.1      ACCGTGAAGTCAGTCAACAAAACGGACAGTCAGACCCTCCTGACCACATTTGGATCTCTG
XM_005258637.1      ------------------------------------------------------TCTCTG
NM_001983.3         ------------------------------------------------------------
XM_005258635.1      ACCGTGAAGTCAGTCAACAAAACGGACAGTCAGACCCTCCTGACCACATTTGGATCTCTG
                                                                                

HsCD00000105        TGACTGAATGTCTGACCACCGTGAAGTCAGTCAACAAAACGGACAGTCAGACCCTCCTGA
NM_001166049.1      ------------------------------------------------------------
NM_202001.2         GAACAGCTCATCGCCGCATCAAGAGAAGATCTGGCCTTATGCCCAGGCCTGGGCCCTCAG
XM_005258634.1      GAACAGCTCATCGCCGCATCAAGAGAAGATCTGGCCTTATGCCCAGGCCTGGGCCCTCAG
XM_005258637.1      GAACAGCTCATCGCCGCATCAAGAGAAGATCTGGCCTTATGCCCAGGCCTGGGCCCTCAG
NM_001983.3         TGACTGAATGTCTGACCACCGTGAAGTCAGTCAACAAAACGGACAGTCAGACCCTCCTGA
XM_005258635.1      GAACAGCTCATCGCCGCATCAAGAGAAGATCTGGCCTTATGCCCAGGCCTGGGCCCTCAG
                                                                                

HsCD00000105        CCACATTTGGATCTCTGGAACAGCTCATCGCCGCATCAAGAGAAGATCTGGCCTTATGCC
NM_001166049.1      -----------TCTCTGGAACAGCTCATCGCCGCATCAAGAGAAGATCTGGCCTTATGCC
NM_202001.2         AAAGTAAGAGCTCTGGGAAAGAACCCAAGGAGTTGGGGGAAGGAGAGAGCCCCAAATAAA
XM_005258634.1      AAAGTAAGAGCTCTGGGAAAGAACCCAAGGAGTTGGGGGAAGGAGAGAGCCCCAAATAAA
XM_005258637.1      AAAGTAAGAGCTCTGGGAAAGAACCCAAGGAGTTGGGGGAAGGAGAGAGCCCCAAATAAA
NM_001983.3         CCACATTTGGATCTCTGGAACAGCTCATCGCCGCATCAAGAGAAGATCTGGCCTTATGCC
XM_005258635.1      AAAGTAAGAGCTCTGGGAAAGAACCCAAGGAGTTGGGGGAAGGAGAGAGCCCCAAATAAA
                               ***  *.** *.* **: *.   .  ...**.*** .   **::**...

HsCD00000105        CAGGCCTGGGCCCTCAGAAAGCCCGGAGGCTGTTTGATGTCCTGCACGAGCCCTTCTTGA
NM_001166049.1      CAGGCCTGGGCCCTCAGAAAGCCCGGAGGCTGTTTGATGTCCTGCACGAGCCCTTCTTGA
NM_202001.2         CACAACCTGAGACCCCAAAGTTTTAAGGTGAAAAAAGAACCAAAGACCAGACACAGTGGC
XM_005258634.1      CACAACCTGAGACCCCAAAGTTTTAAGGTGAAAAAAGAACCAAAGACCAGACACAGTGGC
XM_005258637.1      CACAACCTGAGACCCCAAAGTTTTAAGGTGAAAAAAGAACCAAAGACCAGACACAGTGGC
NM_001983.3         CAGGCCTGGGCCCTCAGAAAGCCCGGAGGCTGTTTGATGTCCTGCACGAGCCCTTCTTGA
XM_005258635.1      CACAACCTGAGACCCCAAAGTTTTAAGGTGAAAAAAGAACCAAAGACCAGACACAGTGGC
                    ** ..*  *. .* *..**.    ...*  :.:::..:. *.:. ** **.*. : * *.

HsCD00000105        AAGTACCCTGA-
NM_001166049.1      AAGTACCCTGA-
NM_202001.2         TTCCGCCTGTAA
XM_005258634.1      TTCCGCCTGTAA
XM_005258637.1      TTCCGCCTGTAA
NM_001983.3         AAGTACCCTGA-
XM_005258635.1      TTCCGCCTGTAA
                    ::  .**   * 

Pathways

Reactome : DNA Repair
Reactome : Dual incision reaction in GG-NER
Reactome : Dual incision reaction in TC-NER
Reactome : Formation of incision complex in GG-NER
Reactome : Formation of transcription-coupled NER (TC-NER) repair complex
Reactome : Global Genomic NER (GG-NER)
Reactome : Nucleotide Excision Repair
Reactome : Transcription-coupled NER (TC-NER)

Protein

UniProt : Q7Z7F5
UniProt : Q96S40
UniProt : P07992
UniProt : B2RC01
HPRD : 00533

Clone

Cloning Information : 31193
HIP Master Clone ID : 61569
Original Clone ID : FLH031193.01X

Organism


TAX_ID : 9606
Species Specific ID: 2067

Genome

Chromosome : 19
Map Location : 19q13.32
Ensembl : ENSG00000012061

Reagents

Labome : ERCC1-antibody