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ITC1 (Saccharomyces cerevisiae) in pDONR221 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  ITC1
Gene Name:  Imitation switch Two Complex 1
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH202973.01X
Keyword: None
Species: Saccharomyces cerevisiae
Type: cDNA
Vector Name: pDONR221               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
ScCD00013239

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 3795nts         Open reading frame : 1 to 3795

ATGGTGTTATATAAAAGGAAACCTATATTACTTCCTGATCCAAAACCTTTACCATTGGAT
TTGAATGTACAAGTATGGCATATTGAAGAGACTGGAGAATGGTTTTCTAGTTACGAAGAA
TTTTTAGAAAGGTTTGATTTCTACACACGCCACCATTTTACATGCGAAATCACTGGTACT
TCTTGCCTGACTTTTTTCCAAGCTCTGGATAGTGAGGAGACACAATTCAAATACGTCGAA
GACAGATTCCCATTAAAGTTGAGAGAACCAGTTGCAAGATTTTTACATTTTAACGGAATA
AGAAGGTTAGATGCATTGGTGGAGAAAGTTTACGCAAGATTTAAAAATGACTTTTTCCCA
GGAGAAGTTGTTTATCTACGTAAACAGAAAGACTCTTCTACCACAAGCTCTAATTCCCAA
CAAAGTACTCCTCAACCTGATGATATGGTCGAAATCAACAGTGTTGGTAATCCTGGCTTA
CCGCAGTACCAATACCAAAGACGTTATGTTATCAAAGAAAAGGTCCAATTTAACGCAACG
ATAAATCCAGAGAGCAGGGAAATTGTGATGCCCGCTCATACTAAATACATGCTGATAGAA
GAAGCAGCTTCCAGTAATAAATCCTTCATTGTTGATCAAGGACAAATTTACAGGGACCGT
TCCACTTTCACTAAGCACTTAATCAAATGTTTTTTCAAGATTACTTTACAGAGGGCATCT
TCGAAAATGGGTGCACCTTGGTGTGTCAAACCGGAATATTTAGCGATGTATGGTTTAACG
ATGGAATGGCCAAAAGATATGTTAAAATATAAAGAAGATGAACCTGTCGTAGCAAGACGT
TCCAACAGTGCCAATGTTTCATCCCCAGAAAGTGAAAAGAATAAACGTCAAAGTAAATCA
TCGGGTAAAAGTAACACCTCAAACGATGCTTCTAACAAAAAAGAAACCAAGAAAAAAAGA
AAGCCAACCGAAGTAAATGATTCTGAGAATAATTCTTCGGAAGAAGACAAGAAGAAGGGT
CAAAATGTCACATCAGAAACACATTCTAAAAAAAGGAAAAAGGAAGCCAATGAGGAACCG
AACACGGAAAACGTCGAAAGCGTTCCTACACCAGCTAATGCGGAGCCTCAGGCTGTAACA
ATAACGAGCATAATGGATGACCTAGCTTTGCCATATCAACATCCACCGAACATCTTTCCA
AACTTAACGTATTATAATGAAAAATTAGAATGTATTTCATTAGGTTCTACAAAACTTTCC
AGACCTTTTGATTCTTTTGGAAAGCTATTACAAGCCTATCAGTTTCTGAATACGTTTGGT
TCGAAAATTTGTCTCTCTCATTTTAGCCTTGACCAATTCATTACTTCTTTGAAATGTACA
GACCCATATGAGTTAAAGGGCGAAGTTGTTTTAGTGAACATAAGAACGCAAACGTCTAAA
GAACAAGAAATTGAAAATAACGGCCTGCCGATGAAAAACAAAGCAGAGACTACTACTGAG
GAAGACTCTGAAAACCCAAGTGACTGGCAAAGAAATTCATTTATTCGTGATATGATAATG
AAAAGAAATTCCGACAAGGTTGAGTACAAGATTGTTCACGATGACCCAGCATCCGATGAT
ATACTTGATAATATCAATCATAATGGGTCTGCTCTTCTCATTGAAGTTTTTACTGCGCTC
TTACGTTTGTTCATCAACGAAGAAGGCGATTGGAGTTGCATCGTCGTTGAAAACTGGATA
ATAGACGACAAGGGAGTCCTTATGGAGAGAAAAGATGAAAGAGGGGAGGGGGAGGCGAAG
CAAAAACGAAATGCTCACGGATACTTTTTACAAGATAAGGAAAAGATCGACAACCTAAAG
GACACATTAAAGGAAAACGCAACAGAAGTTCAAAAAGAATCAGACGCTAAAAACGAAACA
AATTCAGAATCTGATTCTAAATCTGATTCTGACAGTGAAGAACGAGATCCTAAATTAGAA
AAGTGTTTGAACTACCGCAATGTTAACTGGATAGAACGCTTGACGAAAAGGCAATTTAAT
AACAGTTACTGGCTAATAATTTTACTGGGAGTCTTAGAAGATTGTAGACATCTTCCCATG
TATACAGAGTTTATTGATTCTTTCATAGAAAAGATCATACCCAAAGATATATCAGCTACT
CAATTGCCCAAACAGCTTTGGAGAAATTTCTGTCGAAAGCTGTCGTTCAGTGACAAAGTC
AATGCGCTGTGGATACTTGTAGATCTCGTATCGCATTTTTCTCCAGATATCAAAGCTGCT
GTAGATGATTCCATGGAACTATGTGGTCAAATTCGTAGCGAAAGATTCAAAGTTGCGAGA
GAGTTGAAAACGGAGGCAGCCGTCTTAAGTAATCTTCAAGGCGACCTGCAGGCAATTCAA
GAGAAGTTAAATAAAACAGATGAAAACACACCATCAGCCGATGGTGCCGATAAAAAAGAT
GATTCCGAATCAAATAGTGAACCAATTGATCTTATTATCATTGAGAAAAAGCAAAAGTTA
ATTGAAGAGCAGGATAAAAAGGTACAAGCTTTACAATCAGATAAAAATTTCCTCGATAAT
TGTCTATTTGAAAACGATCTACAAAGATTAAAGCCGTTGGGATTGGATCGATACGGGAAC
AGGTATTTTTGGCTGGATCACAATGGTGTTCCATTTCCTCAATATCCTGCTGGTATGAAT
GAGACGCCGAAAAGTAACAATAGTTTGAGTTATCACTCCGGTCGTTTATTAATTCAGGGA
CCTAAAGCATCATCGGCGAAATTCTTTTTGAACGTCAGTGATGAGCAATTGAGCAATTGG
CAAAAAATCAGAAATTCCGAAGGAATATCCGAGGCAACTAGGGAAGTATTTGGTATCTCC
AAAACTAGTTCCGGATCTTATAATTACGTAGAAAATGGAATAGAGGTCGAACTATTGGAC
AGTAATGATCGCGTAAACCCACTTATAGAGCTGACACCTATTCAAAAGAAAATTATGGAT
GAAACACCTTCAAGGTTGCTACTATCACCAGATCAATGGTATTGCATTGACAAGTTAGAG
GACTTGTCAAGAATAATGGACTGGTTAGATAATTGGGGTCGCAAAGAGCATGATCTCTTG
AGGCAAATCAGACCCATAATGGAACGAATTAAAAGTTCCCTTAGCTTGCGCGACCATGCG
CTGTCACTGACAGCATTCACCAAAAATGAAGAAAAGTTGCTAAAGGAGTTAGAAAATAAT
GAATTTACCGAAAACGAATTGAACGTTGATAGTATGGATGTTGATGATAAAAATAGCGGT
GTTAAAAGTGAGGTTGATGTACAGGTTGATGCCGAAGAGAAGAGAGAAGCTGTGATAGAC
GAAAAGTTGGAGGTGATTGCAGACGAACTCATGAAGCTAGATGATAGCTCGAAAACACGC
AATGTTCTTAATAGAATACAAGAATTAGAAGATCAGAGAGATGAACTCTTGGAACAGAAA
AAATCCATAATAAATTCGCAGAGGCCAGGCGCGAGGATTTTAGCCCGTTCAGAAAGGAAA
AGAACCAAAATCTCCCGTGGTAATAAGGTGAATAAGCAGATTGAAATATTGACTGACTTA
GTCAATTATAGACATTTTAAAGCTATGGAAGATGTGATAGCTTGGAAAAACGTTTTAGCA
AATTCGATATGGGGTTCCTCACTTCGCAAAAATGCATCTGGTAATAAAAGGAGTGGTGTC
ATTGAAACAGTAGACGATAAATTAAAAGATATAGTGGGCCAAACCTCAAGAACAGTAACA
CCTGCCCCAAATTAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 3795
Start on reference sequence 1
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Protein
  • Clone
  • Organism

Gene

Target GenBank: CAA96844

 

Clone

Cloning Information : 202973
HIP Master Clone ID : 177684
Original Clone ID : FLH202973.01X

Organism


Species Specific ID: YGL133W