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TIEG (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  KLF10
Gene Name:  Kruppel-like factor 10
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH019290.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / Yes
Publications: None
Authors: HIP
HsCD00000162

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1443nts         Open reading frame : 1 to 1443
Reference Sequence Alignment

ATGCTCAACTTCGGTGCCTCTCTCCAGCAGACTGCGGAGGAAAGAATGGAAATGATTTCT
GAAAGGCCAAAAGAGAGTATGTATTCCTGGAACAAAACTGCAGAGAAAAGTGATTTTGAA
GCTGTAGAAGCACTTATGTCAATGAGCTGCAGTTGGAAGTCTGATTTTAAGAAATACGTT
GAAAACAGACCTGTTACACCAGTATCTGATTTGTCAGAGGAAGAGAATCTGCTTCCGGGA
ACACCTGATTTTCATACAATCCCAGCATTTTGTTTGACTCCACCTTACAGTCCTTCTGAC
TTTGAACCCTCTCAAGTGTCAAATCTGATGGCACCAGCGCCATCTACTGTACACTTCAAG
TCACTCTCAGATACTGCCAAACCTCACATTGCCGCACCTTTCAAAGAGGAAGAAAAGAGC
CCAGTATCTGCCCCCAAACTCCCCAAAGCTCAGGCAACAAGTGTGATTCGTCATACAGCT
GATGCCCAGCTATGTAACCACCAGACCTGCCCAATGAAAGCAGCCAGCATCCTCAACTAT
CAGAACAATTCTTTTAGAAGAAGAACCCACCTAAATGTTGAGGCTGCAAGAAAGAACATA
CCATGTGCCGCTGTGTCACCAAACAGATCCAAATGTGAGAGAAACACAGTGGCAGATGTT
GATGAGAAAGCAAGTGCTGCACTTTATGACTTTTCTGTGCCTTCCTCAGAGACGGTCATC
TGCAGGTCTCAGCCAGCCCCTGTGTCCCCACAACAGAAGTCAGTGTTGGTCTCTCCACCT
GCAGTATCTGCAGGGGGAGTGCCACCTATGCCGGTCATCTGCCAGATGGTTCCCCTTCCT
GCCAACAACCCTGTTGTGACAACAGTCGTTCCCAGCACTCCTCCCAGCCAGCCACCAGCC
GTTTGCCCCCCTGTTGTGTTCATGGGCACACAAGTCCCCAAAGGCGCTGTCATGTTTGTG
GTACCCCAGCCCGTTGTGCAGAGTTCAAAGCCTCCGGTGGTGAGCCCGAATGGCACCAGA
CTCTCTCCCATTGCCCCTGCTCCTGGGTTTTCCCCTTCAGCAGCAAAAGTCACTCCTCAG
ATTGATTCATCAAGGATAAGGAGTCACATCTGTAGCCACCCAGGATGTGGCAAGACATAC
TTTAAAAGTTCCCATCTGAAGGCTCACACGAGGACGCACACAGGAGAAAAGCCTTTCAGC
TGTAGCTGGAAAGGTTGTGAAAGGAGGTTTGCCCGTTCTGATGAACTGTCCAGACACAGG
CGAACCCACACGGGTGAGAAGAAATTTGCGTGCCCCATGTGTGACCGGCGGTTCATGAGG
AGTGACCATTTGACCAAGCATGCCCGGCGCCATCTATCAGCCAAGAAGCTACCAAACTGG
CAGATGGAAGTGAGCAAGCTAAATGACATTGCTCTACCTCCAACCCCTGCTCCCACACAG
TAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1566
Start on reference sequence 124
Mutations:
None
Discrepancy:
c1164t
                                

5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 1015 1048
recombination site loxP site loxP recombination site 9 42
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : KLF10
Symbol Nomenclature : KLF10
SYNONYM : EGR-alpha; EGRA; TIEG; TIEG1
Designation : Krueppel-like factor 10|TGFB-inducible early growth response protein 1|early growth response-alpha|transforming growth factor-beta-inducible early growth response protein 1|zinc finger transcription factor TIEG
Full Nomenclature : Kruppel-like factor 10
GENEID : 7071
GI : 61355041
GenBank Accession : AY888145
HGNC : 11810
MIM : 601878
Vega : OTTHUMG00000164735
Target GenBank: NM_005655

 

Reference Sequence Alignment

  

HsCD00000162        ATGCTCAACTTCGGTGCCTCTCTCCAGCAGACTGCGGAGGAAAGAATGGAAATGATTTCT
NM_005655.3         ATGCTCAACTTCGGTGCCTCTCTCCAGCAGACTGCGGAGGAAAGAATGGAAATGATTTCT
NM_001032282.3      ---------------------------------ATGGAGGAAAGAATGGAAATGATTTCT
                                                     . *************************

HsCD00000162        GAAAGGCCAAAAGAGAGTATGTATTCCTGGAACAAAACTGCAGAGAAAAGTGATTTTGAA
NM_005655.3         GAAAGGCCAAAAGAGAGTATGTATTCCTGGAACAAAACTGCAGAGAAAAGTGATTTTGAA
NM_001032282.3      GAAAGGCCAAAAGAGAGTATGTATTCCTGGAACAAAACTGCAGAGAAAAGTGATTTTGAA
                    ************************************************************

HsCD00000162        GCTGTAGAAGCACTTATGTCAATGAGCTGCAGTTGGAAGTCTGATTTTAAGAAATACGTT
NM_005655.3         GCTGTAGAAGCACTTATGTCAATGAGCTGCAGTTGGAAGTCTGATTTTAAGAAATACGTT
NM_001032282.3      GCTGTAGAAGCACTTATGTCAATGAGCTGCAGTTGGAAGTCTGATTTTAAGAAATACGTT
                    ************************************************************

HsCD00000162        GAAAACAGACCTGTTACACCAGTATCTGATTTGTCAGAGGAAGAGAATCTGCTTCCGGGA
NM_005655.3         GAAAACAGACCTGTTACACCAGTATCTGATTTGTCAGAGGAAGAGAATCTGCTTCCGGGA
NM_001032282.3      GAAAACAGACCTGTTACACCAGTATCTGATTTGTCAGAGGAAGAGAATCTGCTTCCGGGA
                    ************************************************************

HsCD00000162        ACACCTGATTTTCATACAATCCCAGCATTTTGTTTGACTCCACCTTACAGTCCTTCTGAC
NM_005655.3         ACACCTGATTTTCATACAATCCCAGCATTTTGTTTGACTCCACCTTACAGTCCTTCTGAC
NM_001032282.3      ACACCTGATTTTCATACAATCCCAGCATTTTGTTTGACTCCACCTTACAGTCCTTCTGAC
                    ************************************************************

HsCD00000162        TTTGAACCCTCTCAAGTGTCAAATCTGATGGCACCAGCGCCATCTACTGTACACTTCAAG
NM_005655.3         TTTGAACCCTCTCAAGTGTCAAATCTGATGGCACCAGCGCCATCTACTGTACACTTCAAG
NM_001032282.3      TTTGAACCCTCTCAAGTGTCAAATCTGATGGCACCAGCGCCATCTACTGTACACTTCAAG
                    ************************************************************

HsCD00000162        TCACTCTCAGATACTGCCAAACCTCACATTGCCGCACCTTTCAAAGAGGAAGAAAAGAGC
NM_005655.3         TCACTCTCAGATACTGCCAAACCTCACATTGCCGCACCTTTCAAAGAGGAAGAAAAGAGC
NM_001032282.3      TCACTCTCAGATACTGCCAAACCTCACATTGCCGCACCTTTCAAAGAGGAAGAAAAGAGC
                    ************************************************************

HsCD00000162        CCAGTATCTGCCCCCAAACTCCCCAAAGCTCAGGCAACAAGTGTGATTCGTCATACAGCT
NM_005655.3         CCAGTATCTGCCCCCAAACTCCCCAAAGCTCAGGCAACAAGTGTGATTCGTCATACAGCT
NM_001032282.3      CCAGTATCTGCCCCCAAACTCCCCAAAGCTCAGGCAACAAGTGTGATTCGTCATACAGCT
                    ************************************************************

HsCD00000162        GATGCCCAGCTATGTAACCACCAGACCTGCCCAATGAAAGCAGCCAGCATCCTCAACTAT
NM_005655.3         GATGCCCAGCTATGTAACCACCAGACCTGCCCAATGAAAGCAGCCAGCATCCTCAACTAT
NM_001032282.3      GATGCCCAGCTATGTAACCACCAGACCTGCCCAATGAAAGCAGCCAGCATCCTCAACTAT
                    ************************************************************

HsCD00000162        CAGAACAATTCTTTTAGAAGAAGAACCCACCTAAATGTTGAGGCTGCAAGAAAGAACATA
NM_005655.3         CAGAACAATTCTTTTAGAAGAAGAACCCACCTAAATGTTGAGGCTGCAAGAAAGAACATA
NM_001032282.3      CAGAACAATTCTTTTAGAAGAAGAACCCACCTAAATGTTGAGGCTGCAAGAAAGAACATA
                    ************************************************************

HsCD00000162        CCATGTGCCGCTGTGTCACCAAACAGATCCAAATGTGAGAGAAACACAGTGGCAGATGTT
NM_005655.3         CCATGTGCCGCTGTGTCACCAAACAGATCCAAATGTGAGAGAAACACAGTGGCAGATGTT
NM_001032282.3      CCATGTGCCGCTGTGTCACCAAACAGATCCAAATGTGAGAGAAACACAGTGGCAGATGTT
                    ************************************************************

HsCD00000162        GATGAGAAAGCAAGTGCTGCACTTTATGACTTTTCTGTGCCTTCCTCAGAGACGGTCATC
NM_005655.3         GATGAGAAAGCAAGTGCTGCACTTTATGACTTTTCTGTGCCTTCCTCAGAGACGGTCATC
NM_001032282.3      GATGAGAAAGCAAGTGCTGCACTTTATGACTTTTCTGTGCCTTCCTCAGAGACGGTCATC
                    ************************************************************

HsCD00000162        TGCAGGTCTCAGCCAGCCCCTGTGTCCCCACAACAGAAGTCAGTGTTGGTCTCTCCACCT
NM_005655.3         TGCAGGTCTCAGCCAGCCCCTGTGTCCCCACAACAGAAGTCAGTGTTGGTCTCTCCACCT
NM_001032282.3      TGCAGGTCTCAGCCAGCCCCTGTGTCCCCACAACAGAAGTCAGTGTTGGTCTCTCCACCT
                    ************************************************************

HsCD00000162        GCAGTATCTGCAGGGGGAGTGCCACCTATGCCGGTCATCTGCCAGATGGTTCCCCTTCCT
NM_005655.3         GCAGTATCTGCAGGGGGAGTGCCACCTATGCCGGTCATCTGCCAGATGGTTCCCCTTCCT
NM_001032282.3      GCAGTATCTGCAGGGGGAGTGCCACCTATGCCGGTCATCTGCCAGATGGTTCCCCTTCCT
                    ************************************************************

HsCD00000162        GCCAACAACCCTGTTGTGACAACAGTCGTTCCCAGCACTCCTCCCAGCCAGCCACCAGCC
NM_005655.3         GCCAACAACCCTGTTGTGACAACAGTCGTTCCCAGCACTCCTCCCAGCCAGCCACCAGCC
NM_001032282.3      GCCAACAACCCTGTTGTGACAACAGTCGTTCCCAGCACTCCTCCCAGCCAGCCACCAGCC
                    ************************************************************

HsCD00000162        GTTTGCCCCCCTGTTGTGTTCATGGGCACACAAGTCCCCAAAGGCGCTGTCATGTTTGTG
NM_005655.3         GTTTGCCCCCCTGTTGTGTTCATGGGCACACAAGTCCCCAAAGGCGCTGTCATGTTTGTG
NM_001032282.3      GTTTGCCCCCCTGTTGTGTTCATGGGCACACAAGTCCCCAAAGGCGCTGTCATGTTTGTG
                    ************************************************************

HsCD00000162        GTACCCCAGCCCGTTGTGCAGAGTTCAAAGCCTCCGGTGGTGAGCCCGAATGGCACCAGA
NM_005655.3         GTACCCCAGCCCGTTGTGCAGAGTTCAAAGCCTCCGGTGGTGAGCCCGAATGGCACCAGA
NM_001032282.3      GTACCCCAGCCCGTTGTGCAGAGTTCAAAGCCTCCGGTGGTGAGCCCGAATGGCACCAGA
                    ************************************************************

HsCD00000162        CTCTCTCCCATTGCCCCTGCTCCTGGGTTTTCCCCTTCAGCAGCAAAAGTCACTCCTCAG
NM_005655.3         CTCTCTCCCATTGCCCCTGCTCCTGGGTTTTCCCCTTCAGCAGCAAAAGTCACTCCTCAG
NM_001032282.3      CTCTCTCCCATTGCCCCTGCTCCTGGGTTTTCCCCTTCAGCAGCAAAAGTCACTCCTCAG
                    ************************************************************

HsCD00000162        ATTGATTCATCAAGGATAAGGAGTCACATCTGTAGCCACCCAGGATGTGGCAAGACATAC
NM_005655.3         ATTGATTCATCAAGGATAAGGAGTCACATCTGTAGCCACCCAGGATGTGGCAAGACATAC
NM_001032282.3      ATTGATTCATCAAGGATAAGGAGTCACATCTGTAGCCACCCAGGATGTGGCAAGACATAC
                    ************************************************************

HsCD00000162        TTTAAAAGTTCCCATCTGAAGGCTCACACGAGGACGCACACAGGAGAAAAGCCTTTCAGC
NM_005655.3         TTTAAAAGTTCCCATCTGAAGGCCCACACGAGGACGCACACAGGAGAAAAGCCTTTCAGC
NM_001032282.3      TTTAAAAGTTCCCATCTGAAGGCCCACACGAGGACGCACACAGGAGAAAAGCCTTTCAGC
                    *********************** ************************************

HsCD00000162        TGTAGCTGGAAAGGTTGTGAAAGGAGGTTTGCCCGTTCTGATGAACTGTCCAGACACAGG
NM_005655.3         TGTAGCTGGAAAGGTTGTGAAAGGAGGTTTGCCCGTTCTGATGAACTGTCCAGACACAGG
NM_001032282.3      TGTAGCTGGAAAGGTTGTGAAAGGAGGTTTGCCCGTTCTGATGAACTGTCCAGACACAGG
                    ************************************************************

HsCD00000162        CGAACCCACACGGGTGAGAAGAAATTTGCGTGCCCCATGTGTGACCGGCGGTTCATGAGG
NM_005655.3         CGAACCCACACGGGTGAGAAGAAATTTGCGTGCCCCATGTGTGACCGGCGGTTCATGAGG
NM_001032282.3      CGAACCCACACGGGTGAGAAGAAATTTGCGTGCCCCATGTGTGACCGGCGGTTCATGAGG
                    ************************************************************

HsCD00000162        AGTGACCATTTGACCAAGCATGCCCGGCGCCATCTATCAGCCAAGAAGCTACCAAACTGG
NM_005655.3         AGTGACCATTTGACCAAGCATGCCCGGCGCCATCTATCAGCCAAGAAGCTACCAAACTGG
NM_001032282.3      AGTGACCATTTGACCAAGCATGCCCGGCGCCATCTATCAGCCAAGAAGCTACCAAACTGG
                    ************************************************************

HsCD00000162        CAGATGGAAGTGAGCAAGCTAAATGACATTGCTCTACCTCCAACCCCTGCTCCCACACAG
NM_005655.3         CAGATGGAAGTGAGCAAGCTAAATGACATTGCTCTACCTCCAACCCCTGCTCCCACACAG
NM_001032282.3      CAGATGGAAGTGAGCAAGCTAAATGACATTGCTCTACCTCCAACCCCTGCTCCCACACAG
                    ************************************************************

HsCD00000162        TAG
NM_005655.3         TGA
NM_001032282.3      TGA
                    *..

Pathways

WikiPathway : TGF beta Signaling Pathway

Protein

SMART domain : ZnF_C2H2 : zinc finger
UniProt : Q13118
UniProt : O75411
HPRD : 03527

Clone

Cloning Information : 19290
HIP Master Clone ID : 33489
Original Clone ID : FLH019290.01X

Organism


TAX_ID : 9606
Species Specific ID: 7071

Genome

Chromosome : 8
Map Location : 8q22.2
Ensembl : ENSG00000155090

Reagents

Labome : KLF10-antibody