Gene:
Gene Symbol:
topA
Gene Name:
DNA topoisomerase type I, omega protein
Original Clone ID:
FLH140815.01X
Keyword:
None
Species:
Yersinia pestis KIM10+
Type:
cDNA
Vector Name:
pDONR221
Format:
CLOSED
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
HIP
YpCD00016732
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 2616nts
Open reading frame: 1 to 2616
ATGGGTAAAGCTCTCGTAATAGTTGAGTCCCCGGCAAAAGCCAAAACTATTAATAAATAC
TTAGGGAATGATTACGTGGTTAAGTCCAGCGTCGGTCACATTCGTGATTTGCCGACCAGT
GGCTCAGCCAGTAAAAAGAGCGCTAACTCAGCTGAAGAAAAAGCCAAAAAAGACGATAAA
CCCAAAAAGAAAGTGAAGAAAGACGAAAAGGTTGCCTTAGTCAACCGTATGGGGGTCGAT
CCTTATCATGGCTGGAAAGCGCAGTATGAAATTTTGTCTGGCAAAGAGAAAGTGGTTGCC
GAGCTGAAAGCATTAGCAGAAAACGCGGATCATATCTATCTCGCAACCGACCTTGATCGC
GAAGGTGAGGCTATTGCCTGGCATTTGCGGGAAGTGATCGGTGGGGACGATAAACGTTTT
AGCCGCGTTGTCTTTAACGAAATCACCAAGAATGCGATTCAGCAGGCGTTTAATCAGCCT
GGTGAACTGAACATCAGCCGTGTAAATGCACAACAAGCCCGGCGTTTTATGGATCGGGTT
GTTGGCTATATGGTCTCTCCACTGTTGTGGAAGAAAATTGCCCGTGGCCTGTCAGCTGGC
CGTGTACAGTCAGTTGCTGTGCGCCTTGTGGTTGAACGTGAAAAAGAGATTAAAGCATTC
GTTCCTGAAGAATATTGGGAGCTACATGCAGATTTAGTGGCTAAAGGTGACATTCCGATT
CAAATGGAAGTCACTCATGCCCATGGCAAGCCATTTAAACCGGTAAATCGTGAGCAAACA
CATGCAGCGCTGACGTTACTGGAAAATGCCCGTTATAAGGTGTTGGATCGTGAAGATAAA
CCGACCAGCAGTAAGCCCAGCGCACCCTTCATTACCTCCACCTTACAGCAGGCGGCAAGT
ACCCGTCTGAGTTTTGGTGTGAAAAAAACCATGATGATGGCACAGCGCCTGTATGAAGCG
GGTCATATCACCTACATGCGTACCGACTCGACTAATTTGAGTCAGGATGCATTGACGATG
GTACGTGGTTATATCGGCGATAATTTTGGTGATAAGTATCTGCCTGCAACGCCTAACCAA
TACAGCAGCAAAGAGAATTCGCAGGAAGCGCATGAGGCCATCCGCCCTTCAGATGTCAAT
GTGTTAGCTGAGCAGTTGAAAGATATGGAAGCGGATGCCCAGAGGTTGTACCAATTGATT
TGGCGTCAGTTTGTGGCGTGCCAGATGCCTCCGGCCAAGTATGACTCGACCACACTGACA
GTGCAGGCGGGGGATTTCCAACTGCGCGCCAAAGGCCGCACATTGCGCTTTGATGGTTGG
ACCAAGGTTATGCCTGCGCTGCGTAAAGGTGATGAAGATCGCACCTTGCCGGTGATTGAA
GTGGGCAGTGATTTAGACTTACAAAAACTGATTCCGAGCCAGCATTTTACCAAGCCACCG
GCTCGTTATAGTGAAGCATCATTAGTGAAAGAGCTGGAAAAACGGGGTATTGGCCGTCCA
TCGACTTATGCCTCTATTATTTCGACCATTCAAGATCGTGGCTATGTGCGGGTAGAAAAC
CGTCGCTTCTACGCAGAGAAAATGGGTGAAATTGTGACAGACCGCTTGGAAGAAAACTTC
CGCGAGCTGATGAATTACGATTTCACGGCACGAATGGAAAGCGGTTTGGATCAAGTCGCC
AATGATCAGGCCGAATGGAAAGCGGTGCTGGATGGCTTCTTTGCTGAATTCAGCGAGCAG
CTTGAAAAGGCAGAAAAAGATCCTGAAGAAGGAGGAATGCGCCCGAATCAGATGGTGATG
ACCAGCATTGATTGCCCAACCTGTGGCCGTCAGATGGGGATCCGTACTGCGAGTACTGGG
GTGTTCCTTGGCTGTTCCGGTTATGCATTGCCGCCTAAAGAGCGCTGTAAAACCACCATT
AACTTGGTGCCGGACGCTGAGATTTTGAATATTCTGGAGGGTGATGATGCGGAGACTAAC
GCATTACGTGCCCGCCGCCGTTGCCAGAAATGTGGTACTGCGATGGATAGCTACCTTATC
GATAATCAACGCAAGTTACATGTTTGCGGGAATAACCCAGCGTGTGATGGCTATGAAATC
GAAGCAGGTGAGTTCCGGATAAAAGGCTATGAAGGCCCAATTGTTGAGTGCGAGAAATGT
GGTTCTGAAATGCACCTGAAAATGGGGCGCTTCGGTAAGTACATGGGCTGCACCAATGAC
GAGTGTAAGAATACCCGCAAGATTCTGCGTAGCGGTGAAGTTGCACCACCGAAAGAAGAT
CCGGTACCATTGTTAGAGTTGCCTTGCGAGAAATCAGATGCTTATTTCGTCCTACGAGAT
GGCGCAGCTGGCGTGTTCTTGGCAGCCAATACATTCCCTAAATCGCGTGAAACCCGTGCA
CCGCTGGTAGAAGAATTGGTTCGCTTTAAAGACCGTTTGCCTGAGAAGTTGCGTTATCTG
GCTGATGCCCCTGTGGTTGATAAAGAGGGTAATAAAACCCTGGTCCGTTTCAGCCGTAAA
ACGAAGCAACAGTATGTTTCATCAGAGAAAGATGGCAAAGCAACAGGCTGGTCTGCTTTC
TATATTGATGGTAAATGGGTTGAGGCAAAGAAATAG
Protein sequence predicted based on nucleotide sequence
MGKALVIVESPAKAKTINKYLGNDYVVKSSVGHIRDLPTSGSASKKSANSAEEKAKKDDK PKKKVKKDEKVALVNRMGVDPYHGWKAQYEILSGKEKVVAELKALAENADHIYLATDLDR EGEAIAWHLREVIGGDDKRFSRVVFNEITKNAIQQAFNQPGELNISRVNAQQARRFMDRV VGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREKEIKAFVPEEYWELHADLVAKGDIPI QMEVTHAHGKPFKPVNREQTHAALTLLENARYKVLDREDKPTSSKPSAPFITSTLQQAAS TRLSFGVKKTMMMAQRLYEAGHITYMRTDSTNLSQDALTMVRGYIGDNFGDKYLPATPNQ YSSKENSQEAHEAIRPSDVNVLAEQLKDMEADAQRLYQLIWRQFVACQMPPAKYDSTTLT VQAGDFQLRAKGRTLRFDGWTKVMPALRKGDEDRTLPVIEVGSDLDLQKLIPSQHFTKPP ARYSEASLVKELEKRGIGRPSTYASIISTIQDRGYVRVENRRFYAEKMGEIVTDRLEENF RELMNYDFTARMESGLDQVANDQAEWKAVLDGFFAEFSEQLEKAEKDPEEGGMRPNQMVM TSIDCPTCGRQMGIRTASTGVFLGCSGYALPPKERCKTTINLVPDAEILNILEGDDAETN ALRARRRCQKCGTAMDSYLIDNQRKLHVCGNNPACDGYEIEAGEFRIKGYEGPIVECEKC GSEMHLKMGRFGKYMGCTNDECKNTRKILRSGEVAPPKEDPVPLLELPCEKSDAYFVLRD GAAGVFLAANTFPKSRETRAPLVEELVRFKDRLPEKLRYLADAPVVDKEGNKTLVRFSRK TKQQYVSSEKDGKATGWSAFYIDGKWVEAKK*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
1
End on reference sequence
2616
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
