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STK17B (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  STK17B
Gene Name:  serine/threonine kinase 17b
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH000142.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / Yes
Publications: PMID: 16512675
Title: Functional proteomics approach to investigate the biological activities of cDNAs implicated in breast cancer.
PMID: 15928075
Title: Building a human kinase gene repository: bioinformatics, molecular cloning, and functional validation.
Authors: HIP
HsCD00000180

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1119nts         Open reading frame : 1 to 1119
Reference Sequence Alignment

ATGTCGAGGAGGAGATTTGATTGCCGAAGTATTTCAGGCCTACTAACTACAACTCCTCAA
ATTCCAATAAAAATGGAAAACTTTAATAATTTCTATATACTTACATCTAAAGAGCTAGGG
AGAGGAAAATTTGCTGTGGTTAGACAATGTATATCAAAATCTACTGGCCAAGAATATGCT
GCAAAATTTCTAAAAAAGAGAAGAAGAGGACAGGATTGTCGAGCAGAAATTTTACACGAG
ATTGCTGTGCTTGAATTGGCAAAGTCTTGTCCCCGTGTTATTAATCTTCATGAGGTCTAT
GAAAATACAAGTGAAATCATTTTGATATTGGAATATGCTGCAGGTGGAGAAATTTTCAGC
CTGTGTTTACCTGAGTTGGCTGAAATGGTTTCTGAAAATGATGTTATCAGACTCATTAAA
CAAATACTTGAAGGAGTTTATTATCTACATCAGAATAACATTGTACACCTTGATTTAAAG
CCACAGAATATATTACTGAGCAGCATATACCCTCTCGGGGACATTAAAATAGTAGATTTT
GGAATGTCTCGAAAAATAGGGCATGCGTGTGAACTTCGGGAAATCATGGGAACACCAGAA
TATTTAGCTCCAGAAATCCTGAACTATGATCCCATTACCACAGCAACAGATATGTGGAAT
ATTGGTATAATAGCATATATGTTGTTAACTCACACATCACCATTTGTGGGAGAAGATAAT
CAAGAAACATACCTCAATATTTCTCAAGTTAATGTAGATTATTCGGAAGAAACTTTTTCA
TCAGTTTCACAGCTGGCCACAGACTTTATTCAGAGCCTTTTAGTAAAAAATCCAGAGAAA
AGACCAACAGCAGAGATATGCCTTTCTCATTCTTGGCTACAGCAGTGGGACTTTGAAAAC
TTGTTTCACCCTGAAGAAACTTCCAGTTCCTCTCAAACTCAGGATCATTCTGTAAGGTCC
TCTGAAGACAAGACTTCTAAATCCTCCTGTAATGGAACCTGTGGTGATAGAGAAGACAAA
GAGAATATCCCAGAGGATAGCAGCATGGTTTCCAAAAGATTTCGTTTCGATGACTCATTA
CCCAATCCCCATGAACTTGTTTCAGATTTGCTCTGTTTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1380
Start on reference sequence 262
Mutations:
None
Discrepancy:
g222a
                                

5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 1015 1048
recombination site loxP site loxP recombination site 9 42
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : STK17B
Symbol Nomenclature : STK17B
SYNONYM : DRAK2
Designation : DAP kinase-related apoptosis-inducing protein kinase 2|death-associated protein kinase-related 2|serine/threonine-protein kinase 17B
Full Nomenclature : serine/threonine kinase 17b
GENEID : 9262
GI : 33304160
GenBank Accession : AY335761
HGNC : 11396
MIM : 604727
Vega : OTTHUMG00000132735
Target GenBank: NM_004226

 

Reference Sequence Alignment

  

HsCD00000180      ATGTCGAGGAGGAGATTTGATTGCCGAAGTATTTCAGGCCTACTAACTACAACTCCTCAA
NM_004226.3       ATGTCGAGGAGGAGATTTGATTGCCGAAGTATTTCAGGCCTACTAACTACAACTCCTCAA
                  ************************************************************

HsCD00000180      ATTCCAATAAAAATGGAAAACTTTAATAATTTCTATATACTTACATCTAAAGAGCTAGGG
NM_004226.3       ATTCCAATAAAAATGGAAAACTTTAATAATTTCTATATACTTACATCTAAAGAGCTAGGG
                  ************************************************************

HsCD00000180      AGAGGAAAATTTGCTGTGGTTAGACAATGTATATCAAAATCTACTGGCCAAGAATATGCT
NM_004226.3       AGAGGAAAATTTGCTGTGGTTAGACAATGTATATCAAAATCTACTGGCCAAGAATATGCT
                  ************************************************************

HsCD00000180      GCAAAATTTCTAAAAAAGAGAAGAAGAGGACAGGATTGTCGAGCAGAAATTTTACACGAG
NM_004226.3       GCAAAATTTCTAAAAAAGAGAAGAAGAGGACAGGATTGTCGAGCAGAAATTTTACACGAG
                  ************************************************************

HsCD00000180      ATTGCTGTGCTTGAATTGGCAAAGTCTTGTCCCCGTGTTATTAATCTTCATGAGGTCTAT
NM_004226.3       ATTGCTGTGCTTGAATTGGCAAAGTCTTGTCCCCGTGTTATTAATCTTCATGAGGTCTAT
                  ************************************************************

HsCD00000180      GAAAATACAAGTGAAATCATTTTGATATTGGAATATGCTGCAGGTGGAGAAATTTTCAGC
NM_004226.3       GAAAATACAAGTGAAATCATTTTGATATTGGAATATGCTGCAGGTGGAGAAATTTTCAGC
                  ************************************************************

HsCD00000180      CTGTGTTTACCTGAGTTGGCTGAAATGGTTTCTGAAAATGATGTTATCAGACTCATTAAA
NM_004226.3       CTGTGTTTACCTGAGTTGGCTGAAATGGTTTCTGAAAATGATGTTATCAGACTCATTAAA
                  ************************************************************

HsCD00000180      CAAATACTTGAAGGAGTTTATTATCTACATCAGAATAACATTGTACACCTTGATTTAAAG
NM_004226.3       CAAATACTTGAAGGAGTTTATTATCTACATCAGAATAACATTGTACACCTTGATTTAAAG
                  ************************************************************

HsCD00000180      CCACAGAATATATTACTGAGCAGCATATACCCTCTCGGGGACATTAAAATAGTAGATTTT
NM_004226.3       CCACAGAATATATTACTGAGCAGCATATACCCTCTCGGGGACATTAAAATAGTAGATTTT
                  ************************************************************

HsCD00000180      GGAATGTCTCGAAAAATAGGGCATGCGTGTGAACTTCGGGAAATCATGGGAACACCAGAA
NM_004226.3       GGAATGTCTCGAAAAATAGGGCATGCGTGTGAACTTCGGGAAATCATGGGAACACCAGAA
                  ************************************************************

HsCD00000180      TATTTAGCTCCAGAAATCCTGAACTATGATCCCATTACCACAGCAACAGATATGTGGAAT
NM_004226.3       TATTTAGCTCCAGAAATCCTGAACTATGATCCCATTACCACAGCAACAGATATGTGGAAT
                  ************************************************************

HsCD00000180      ATTGGTATAATAGCATATATGTTGTTAACTCACACATCACCATTTGTGGGAGAAGATAAT
NM_004226.3       ATTGGTATAATAGCATATATGTTGTTAACTCACACATCACCATTTGTGGGAGAAGATAAT
                  ************************************************************

HsCD00000180      CAAGAAACATACCTCAATATTTCTCAAGTTAATGTAGATTATTCGGAAGAAACTTTTTCA
NM_004226.3       CAAGAAACATACCTCAATATTTCTCAAGTTAATGTAGATTATTCGGAAGAAACTTTTTCA
                  ************************************************************

HsCD00000180      TCAGTTTCACAGCTGGCCACAGACTTTATTCAGAGCCTTTTAGTAAAAAATCCAGAGAAA
NM_004226.3       TCAGTTTCACAGCTGGCCACAGACTTTATTCAGAGCCTTTTAGTAAAAAATCCAGAGAAA
                  ************************************************************

HsCD00000180      AGACCAACAGCAGAGATATGCCTTTCTCATTCTTGGCTACAGCAGTGGGACTTTGAAAAC
NM_004226.3       AGACCAACAGCAGAGATATGCCTTTCTCATTCTTGGCTACAGCAGTGGGACTTTGAAAAC
                  ************************************************************

HsCD00000180      TTGTTTCACCCTGAAGAAACTTCCAGTTCCTCTCAAACTCAGGATCATTCTGTAAGGTCC
NM_004226.3       TTGTTTCACCCTGAAGAAACTTCCAGTTCCTCTCAAACTCAGGATCATTCTGTAAGGTCC
                  ************************************************************

HsCD00000180      TCTGAAGACAAGACTTCTAAATCCTCCTGTAATGGAACCTGTGGTGATAGAGAAGACAAA
NM_004226.3       TCTGAAGACAAGACTTCTAAATCCTCCTGTAATGGAACCTGTGGTGATAGAGAAGACAAA
                  ************************************************************

HsCD00000180      GAGAATATCCCAGAGGATAGCAGCATGGTTTCCAAAAGATTTCGTTTCGATGACTCATTA
NM_004226.3       GAGAATATCCCAGAGGATAGCAGCATGGTTTCCAAAAGATTTCGTTTCGATGACTCATTA
                  ************************************************************

HsCD00000180      CCCAATCCCCATGAACTTGTTTCAGATTTGCTCTGTTTG
NM_004226.3       CCCAATCCCCATGAACTTGTTTCAGATTTGCTCTGTTAG
                  *************************************:*

Clone

Cloning Information : 142
HIP Master Clone ID : 87200
Original Clone ID : FLH000142.01L

Organism


TAX_ID : 9606
Species Specific ID: 9262

Genome

Chromosome : 2
Map Location : 2q32.3
Ensembl : ENSG00000081320

Reagents

Labome : STK17B-antibody