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RPS5 (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  RPS5
Gene Name:  ribosomal protein S5
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH027247.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: PMID: 16512675
Title: Functional proteomics approach to investigate the biological activities of cDNAs implicated in breast cancer.
Authors: HIP
HsCD00001986

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 615nts         Open reading frame : 1 to 615
Reference Sequence Alignment

ATGACCGAGTGGGAGACAGCAGCACCAGCGGTGGCAGAGACCCCAGACATCAAGCTCTTT
GGGAAGTGGAGCACCGATGATGTGCAGATCAATGACATTTCCCTGCAGGATTACATTGCA
GTGAAGGAGAAGTATGCCAAGTACCTGCCTCACAGTGCAGGGCGGTATGCCGCCAAACGC
TTCCGCAAAGCTCAGTGTCCCATTGTGGAGCGCCTCACTAACTCCATGATGATGCACGGC
CGCAACAACGGCAAGAAGCTCATGACTGTGCGCATCGTCAAGCATGCCTTCGAGATCATA
CACCTGCTCACAGGCGAGAACCCTCTGCAGGTCCTGGTGAACGCCATCATCAACAGTGGT
CCCCGGGAGGACTCCACACGCATTGGGCGCGCCGGGACTGTGAGACGACAGGCTGTGGAT
GTGTCCCCCCTGCGCCGTGTGAACCAGGCCATCTGGCTGCTGTGCACAGGCGCTCGTGAG
GCTGCCTTCCGGAACATTAAGACCATTGCTGAGTGCCTGGCAGATGAGCTCATCAATGCT
GCCAAGGGCTCCTCGAACTCCTATGCCATTAAGAAGAAGGACGAGCTGGAGCGTGTGGCC
AAGTCCAACCGCTTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 675
Start on reference sequence 61
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 1015 1048
recombination site loxP site loxP recombination site 9 42
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : RPS5
Symbol Nomenclature : RPS5
SYNONYM : S5
Designation : 40S ribosomal protein S5
Full Nomenclature : ribosomal protein S5
GENEID : 6193
GI : 61369835
GenBank Accession : AY891473
HGNC : 10426
MIM : 603630
Vega : OTTHUMG00000183530
Target GenBank: BC015405

 

Reference Sequence Alignment

  

HsCD00001986      ATGACCGAGTGGGAGACAGCAGCACCAGCGGTGGCAGAGACCCCAGACATCAAGCTCTTT
NM_001009.3       ATGACCGAGTGGGAGACAGCAGCACCAGCGGTGGCAGAGACCCCAGACATCAAGCTCTTT
                  ************************************************************

HsCD00001986      GGGAAGTGGAGCACCGATGATGTGCAGATCAATGACATTTCCCTGCAGGATTACATTGCA
NM_001009.3       GGGAAGTGGAGCACCGATGATGTGCAGATCAATGACATTTCCCTGCAGGATTACATTGCA
                  ************************************************************

HsCD00001986      GTGAAGGAGAAGTATGCCAAGTACCTGCCTCACAGTGCAGGGCGGTATGCCGCCAAACGC
NM_001009.3       GTGAAGGAGAAGTATGCCAAGTACCTGCCTCACAGTGCAGGGCGGTATGCCGCCAAACGC
                  ************************************************************

HsCD00001986      TTCCGCAAAGCTCAGTGTCCCATTGTGGAGCGCCTCACTAACTCCATGATGATGCACGGC
NM_001009.3       TTCCGCAAAGCTCAGTGTCCCATTGTGGAGCGCCTCACTAACTCCATGATGATGCACGGC
                  ************************************************************

HsCD00001986      CGCAACAACGGCAAGAAGCTCATGACTGTGCGCATCGTCAAGCATGCCTTCGAGATCATA
NM_001009.3       CGCAACAACGGCAAGAAGCTCATGACTGTGCGCATCGTCAAGCATGCCTTCGAGATCATA
                  ************************************************************

HsCD00001986      CACCTGCTCACAGGCGAGAACCCTCTGCAGGTCCTGGTGAACGCCATCATCAACAGTGGT
NM_001009.3       CACCTGCTCACAGGCGAGAACCCTCTGCAGGTCCTGGTGAACGCCATCATCAACAGTGGT
                  ************************************************************

HsCD00001986      CCCCGGGAGGACTCCACACGCATTGGGCGCGCCGGGACTGTGAGACGACAGGCTGTGGAT
NM_001009.3       CCCCGGGAGGACTCCACACGCATTGGGCGCGCCGGGACTGTGAGACGACAGGCTGTGGAT
                  ************************************************************

HsCD00001986      GTGTCCCCCCTGCGCCGTGTGAACCAGGCCATCTGGCTGCTGTGCACAGGCGCTCGTGAG
NM_001009.3       GTGTCCCCCCTGCGCCGTGTGAACCAGGCCATCTGGCTGCTGTGCACAGGCGCTCGTGAG
                  ************************************************************

HsCD00001986      GCTGCCTTCCGGAACATTAAGACCATTGCTGAGTGCCTGGCAGATGAGCTCATCAATGCT
NM_001009.3       GCTGCCTTCCGGAACATTAAGACCATTGCTGAGTGCCTGGCAGATGAGCTCATCAATGCT
                  ************************************************************

HsCD00001986      GCCAAGGGCTCCTCGAACTCCTATGCCATTAAGAAGAAGGACGAGCTGGAGCGTGTGGCC
NM_001009.3       GCCAAGGGCTCCTCGAACTCCTATGCCATTAAGAAGAAGGACGAGCTGGAGCGTGTGGCC
                  ************************************************************

HsCD00001986      AAGTCCAACCGCTTG
NM_001009.3       AAGTCCAACCGCTGA
                  ************* .

Pathways

Reactome : 3' -UTR-mediated translational regulation
Reactome : Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S
Reactome : Cap-dependent Translation Initiation
Reactome : Disease
Reactome : Eukaryotic Translation Elongation
Reactome : Eukaryotic Translation Initiation
Reactome : Eukaryotic Translation Termination
Reactome : Formation of a pool of free 40S subunits
Reactome : Formation of the ternary complex, and subsequently, the 43S complex
Reactome : GTP hydrolysis and joining of the 60S ribosomal subunit
Reactome : Gene Expression
Reactome : Influenza Infection
Reactome : Influenza Life Cycle
Reactome : Influenza Viral RNA Transcription and Replication
Reactome : L13a-mediated translational silencing of Ceruloplasmin expression
Reactome : Metabolism of RNA
Reactome : Metabolism of mRNA
Reactome : Metabolism of proteins
Reactome : Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC)
Reactome : Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC)
Reactome : Nonsense-Mediated Decay (NMD)
Reactome : Peptide chain elongation
Reactome : Ribosomal scanning and start codon recognition
Reactome : SRP-dependent cotranslational protein targeting to membrane
Reactome : Translation
Reactome : Translation initiation complex formation
Reactome : Viral mRNA Translation
WikiPathway : Cytoplasmic Ribosomal Proteins

Protein

UniProt : P46782
HPRD : 04695

Clone

Cloning Information : 27247
HIP Master Clone ID : 57623
Original Clone ID : FLH027247.01L

Organism


TAX_ID : 9606
Species Specific ID: 6193

Genome

Chromosome : 19
Map Location : 19q13.4
Ensembl : ENSG00000083845

Reagents

Labome : RPS5-antibody