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UBI4 (Saccharomyces cerevisiae) in pGEX-2T-K

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Explanation of Terms


Gene: Gene Symbol:  UBI4
Gene Name:  Ubiquitin, becomes conjugated to proteins, marking them for selective degradation via the ubiquitin-26S proteasome system; essential for the cellular stress response
Sequence              Map: 4027.pdf
Original Clone ID: 4027
Keyword: None
Species: Saccharomyces cerevisiae
Type: cDNA
Vector Name: pGEX-2T-K               Format:  CLOSED
Source: Peter Howley
Description: None
Comments: cloned BamHI (5p) to EcoRI (3p)
Mutation/
Discrepancy :
Yes / Unknown
Publications: PMID: 7708685
Title: A family of proteins structurally and functionally related to the E6-AP ubiquitin-protein ligase
PMID: 8221889
Title: The HPV-16 E6 and E6-AP complex functions as a ubiquitin-protein ligase in the ubiquitination of p53
Authors: Rebecca Kramer
Peter Howley
J Huibregtse
ScCD00021236

Price:  Login for Pricing
AVAILABLE
CPD only
Special MTA: None

 


Coding Sequence Details
Insert Sequence Verified?: U Verification Method: 
Mutations:


Discrepancy:
None
Others:
Tag GST (from vector)
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : EB5-alpha T1, T5 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pGEX-2T-K

pGEX-2T-K in advanced viewed

Synonyms: pGEX-2TK
Sequencing Primer: Forward:  Unknown
Reverse:  Unknown
Description: pGEX-series GST fusion vector with Ptac promoter, lacI repressor, thrombin cleavage and kinase site ORFS; amp resistance; restriction enzyme cloning.
Comments: None
Size (bp): 4969
Parent Vector: None
Empty Vector: None
Properties: bacterial expression, with tag/fusion/marker
Author Name: Amersham Pharmacia
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS MCS 0 0
bacterial origin pMB1 ori pMB1 (ColE1 and pBR322) bacterial origin 0 0
repressor protein gene lacI lacI repressor gene 0 0
selectable marker AmpR ampicillin resistance gene (bla) 0 0
tag GST GST fusion protein ORF with thrombin cleavage site 0 0

 


Original Clone ID: 4027
SYNONYM: GST-ubiquitin
SYNONYM: pGEX-2TK-UBI4 ORF
Species Specific ID: YLL039C
Target GenBank: NP_013061