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BTRC (Homo sapiens) in pcDNA3

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Explanation of Terms


Gene: Gene Symbol:  BTRC
Gene Name:  beta-transducin repeat containing E3 ubiquitin protein ligase
Sequence              Map: 4489.pdf
Original Clone ID: 4489
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pcDNA3               Format:  CLOSED
Source: Peter Howley
Description: None
Comments: Removed from collection because insert is incorrect
Mutation/
Discrepancy :
No / Unknown
Publications: PMID: 9660940
Title: A novel human WD protein, h-beta TrCp, that interacts with HIV-1 Vpu connects CD4 to the ER degradation pathway through an F-box motif
PMID: 11030355
Title: Harnessing the ubiquitination machinery to target the degradation of specific cellular proteins
Authors: Rebecca Kramer
Peter Howley
P Zhou
HsCD00021237

Price:  Login for Pricing
NOT AVAILABLE
CPD only
Special MTA: None

 


Coding Sequence Details
Insert Sequence Verified?: U Verification Method: 
Mutations:
None
Discrepancy:
None
Others:
Tag Flag
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : EB5-alpha T1, T5 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  neomycin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pcDNA3

pcDNA3 in advanced viewed

Synonyms: pcDNA-3
Sequencing Primer: Forward:  Unknown
Reverse:  Unknown
Description: Mammalian expression vector with CMV promoter, BGH polyA signal, T7, SP6; amp resistance (neomycin for mammalian selection); restriction enzyme cloning.
Comments: None
Size (bp): 5446
Parent Vector: None
Empty Vector: None
Properties: in vitro transcription, mammalian expression, multiple cloning site, ssDNA production
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS MCS (HindIII KpnI BamHI BstXI EcoRI EcoRV BstXI NotI XhoI XbaI ApaI) 0 0
bacterial origin ColE1 ColE1 origin of replication 0 0
poly-A signal SV40 polyA SV40 polyadenylation signal sequence (for neoR) 0 0
poly-A signal BGH polyA BGH polyadenylation signal sequence 0 0
promoter SP6 SP6 prokaryotic promoter 0 0
promoter T7 T7 prokaryotic promoter 0 0
promoter CMV CMV promoter 0 0
promoter SV40 SV40 promoter (for neoR) 0 0
selectable marker AmpR ampicillin resistance 0 0
selectable marker NeoR neomycin resistance gene 0 0
viral origin SV40 SV40 origin (with SV40 promoter) 0 0

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome

Gene

 

Gene Symbol : BTRC
Symbol Nomenclature : BTRC
SYNONYM : BETA-TRCP; FBW1A; FBXW1; FBXW1A; FWD1; bTrCP; bTrCP1; betaTrCP
Designation : E3RSIkappaB|F-box and WD repeats protein beta-TrCP|F-box and WD-repeat protein 1B|F-box/WD repeat-containing protein 1A|beta-TrCP1|epididymis tissue protein Li 2a|pIkappaBalpha-E3 receptor subunit
Full Nomenclature : beta-transducin repeat containing E3 ubiquitin protein ligase
GENEID : 8945
Locus Tag : RP11-529I10.2
SYNONYM : pcDNA-F-beta-Trcp
SYNONYM : pcDNA3-FLAG-BTRC
HGNC : 1144
MIM : 603482
Vega : OTTHUMG00000018932
Target GenBank: None

 

Pathways

Panther : Hedgehog signaling pathway
Panther : Wnt signaling pathway
Reactome : APC/C-mediated degradation of cell cycle proteins
Reactome : Activation of NF-kappaB in B cells
Reactome : Adaptive Immune System
Reactome : Antigen processing: Ubiquitination & Proteasome degradation
Reactome : Cell Cycle
Reactome : Cell Cycle, Mitotic
Reactome : Circadian Clock
Reactome : Class I MHC mediated antigen processing & presentation
Reactome : Cytokine Signaling in Immune system
Reactome : Disease
Reactome : Downstream signaling events of B Cell Receptor (BCR)
Reactome : G2/M Transition
Reactome : HIV Infection
Reactome : Host Interactions of HIV factors
Reactome : Immune System
Reactome : Interleukin-1 signaling
Reactome : Mitotic G2-G2/M phases
Reactome : Nuclear signaling by ERBB4
Reactome : Prolactin receptor signaling
Reactome : Regulation of APC/C activators between G1/S and early anaphase
Reactome : Regulation of PLK1 Activity at G2/M Transition
Reactome : Regulation of mitotic cell cycle
Reactome : SCF-beta-TrCP mediated degradation of Emi1
Reactome : Signal Transduction
Reactome : Signaling by ERBB4
Reactome : Signaling by Interleukins
Reactome : Signaling by Wnt
Reactome : Signaling by the B Cell Receptor (BCR)
Reactome : TCF dependent signaling in response to WNT
Reactome : Vpu mediated degradation of CD4
Reactome : deactivation of the beta-catenin transactivating complex
WikiPathway : TGF beta Signaling Pathway
WikiPathway : TNF alpha Signaling Pathway

Protein

HPRD : 04596

Clone

Original Clone ID : 4489

Organism


TAX_ID : 9606
Species Specific ID: 8945

Genome

Chromosome : 10
Map Location : 10q24.32
Ensembl : ENSG00000166167