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CRADD (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  CRADD
Gene Name:  CASP2 and RIPK1 domain containing adaptor with death domain
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH109918.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00002206

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 600nts         Open reading frame : 1 to 600
Reference Sequence Alignment

ATGGAGGCCAGAGACAAACAAGTACTCCGCTCACTTCGCCTGGAGCTGGGTGCAGAGGTA
TTGGTGGAGGGACTGGTTCTTCAGTACCTCTACCAGGAAGGAATCTTGACGGAAAACCAT
ATTCAAGAAATCAATGCTCAAACCACAGGCCTCCGGAAAACAATGCTCCTGCTGGATATC
CTACCTTCCAGGGGCCCTAAAGCATTTGATACATTCCTAGATTCCCTACAGGAGTTTCCC
TGGGTCAGGGAGAAGCTGAAGAAGGCAAGGGAAGAGGCCATGACCGACCTGCCTGCAGGT
GACAGATTGACTGGGATCCCCTCGCACATCCTCAACAGCTCCCCATCAGACCGGCAGATT
AACCAGCTGGCCCAGAGGCTGGGCCCTGAGTGGGAGCCCATGGTGCTGTCTCTGGGACTG
TCCCAGACGGATATCTACCGCTGTAAGGCCAACCACCCCCACAACGTGCAGTCGCAGGTG
GTGGAGGCCTTCATCCGTTGGCGGCAGCGCTTCGGGAAGCAGGCCACCTTCCAGAGCCTG
CACAACGGGCTGCGGGCTGTGGAGGTGGACCCCTCGCTGCTCCTGCACATGTTGGAGTAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 680
Start on reference sequence 81
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 1015 1048
recombination site loxP site loxP recombination site 9 42
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CRADD
Symbol Nomenclature : CRADD
SYNONYM : MRT34; RAIDD
Designation : RIP-associated ICH1/CED3-homologous protein with death domain|RIP-associated protein with a death domain|caspase and RIP adaptor with death domain|death adaptor molecule RAIDD|death domain-containing protein CRADD
Full Nomenclature : CASP2 and RIPK1 domain containing adaptor with death domain
GENEID : 8738
GI : 61360687
GenBank Accession : AY888963
HGNC : 2340
MIM : 603454
Vega : OTTHUMG00000170322
Target GenBank: BC017042

 

Reference Sequence Alignment

  

HsCD00002206      ATGGAGGCCAGAGACAAACAAGTACTCCGCTCACTTCGCCTGGAGCTGGGTGCAGAGGTA
NM_003805.3       ATGGAGGCCAGAGACAAACAAGTACTCCGCTCACTTCGCCTGGAGCTGGGTGCAGAGGTA
                  ************************************************************

HsCD00002206      TTGGTGGAGGGACTGGTTCTTCAGTACCTCTACCAGGAAGGAATCTTGACGGAAAACCAT
NM_003805.3       TTGGTGGAGGGACTGGTTCTTCAGTACCTCTACCAGGAAGGAATCTTGACGGAAAACCAT
                  ************************************************************

HsCD00002206      ATTCAAGAAATCAATGCTCAAACCACAGGCCTCCGGAAAACAATGCTCCTGCTGGATATC
NM_003805.3       ATTCAAGAAATCAATGCTCAAACCACAGGCCTCCGGAAAACAATGCTCCTGCTGGATATC
                  ************************************************************

HsCD00002206      CTACCTTCCAGGGGCCCTAAAGCATTTGATACATTCCTAGATTCCCTACAGGAGTTTCCC
NM_003805.3       CTACCTTCCAGGGGCCCTAAAGCATTTGATACATTCCTAGATTCCCTACAGGAGTTTCCC
                  ************************************************************

HsCD00002206      TGGGTCAGGGAGAAGCTGAAGAAGGCAAGGGAAGAGGCCATGACCGACCTGCCTGCAGGT
NM_003805.3       TGGGTCAGGGAGAAGCTGAAGAAGGCAAGGGAAGAGGCCATGACCGACCTGCCTGCAGGT
                  ************************************************************

HsCD00002206      GACAGATTGACTGGGATCCCCTCGCACATCCTCAACAGCTCCCCATCAGACCGGCAGATT
NM_003805.3       GACAGATTGACTGGGATCCCCTCGCACATCCTCAACAGCTCCCCATCAGACCGGCAGATT
                  ************************************************************

HsCD00002206      AACCAGCTGGCCCAGAGGCTGGGCCCTGAGTGGGAGCCCATGGTGCTGTCTCTGGGACTG
NM_003805.3       AACCAGCTGGCCCAGAGGCTGGGCCCTGAGTGGGAGCCCATGGTGCTGTCTCTGGGACTG
                  ************************************************************

HsCD00002206      TCCCAGACGGATATCTACCGCTGTAAGGCCAACCACCCCCACAACGTGCAGTCGCAGGTG
NM_003805.3       TCCCAGACGGATATCTACCGCTGTAAGGCCAACCACCCCCACAACGTGCAGTCGCAGGTG
                  ************************************************************

HsCD00002206      GTGGAGGCCTTCATCCGTTGGCGGCAGCGCTTCGGGAAGCAGGCCACCTTCCAGAGCCTG
NM_003805.3       GTGGAGGCCTTCATCCGTTGGCGGCAGCGCTTCGGGAAGCAGGCCACCTTCCAGAGCCTG
                  ************************************************************

HsCD00002206      CACAACGGGCTGCGGGCTGTGGAGGTGGACCCCTCGCTGCTCCTGCACATGTTGGAGTAG
NM_003805.3       CACAACGGGCTGCGGGCTGTGGAGGTGGACCCCTCGCTGCTCCTGCACATGTTGGAGTGA
                  **********************************************************..

Pathways

NCI : Caspase Cascade in Apoptosis
NCI : Ceramide signaling pathway
NCI : HIV-1 Nef: Negative effector of Fas and TNF-alpha
Panther : Apoptosis signaling pathway
WikiPathway : Apoptosis
WikiPathway : Apoptosis Modulation and Signaling

Clone

Cloning Information : 109918
HIP Master Clone ID : 86201
Original Clone ID : FLH109918.01X

Organism


TAX_ID : 9606
Species Specific ID: 8738

Genome

Chromosome : 12
Map Location : 12q21.33-q23.1
Ensembl : ENSG00000169372

Reagents

Labome : CRADD-antibody