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SFRP1 (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  SFRP1
Gene Name:  secreted frizzled-related protein 1
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH019929.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / Yes
Publications: PMID: 16512675
Title: Functional proteomics approach to investigate the biological activities of cDNAs implicated in breast cancer.
Authors: HIP
HsCD00022108

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 945nts         Open reading frame : 1 to 945
Reference Sequence Alignment

ATGGGCATCGGGCGCAGCGAGGGGGGCCGCCGCGGGGCAGCCCTGGGCGTGCTGCTGGCG
CTGGGCGCGGCGCTTCTGGCCGTGGGCTCGGCCAGCGAGTACGACTACGTGAGCTTCCAG
TCGGACATCGGCCCGTACCAGAGCGGGCGCTTCTACACCAAGCCACCTCAGTGCGTGGAC
ATCCCCGCGGACCTGCGGCTGTGCCACAACGTGGGCTACAAGAAGATGGTGCTGCCCAAC
CTGCTGGAGCACGAGACCATGGCGGAGGTGAAGCAGCAGGCCAGCAGCTGGGTGCCCCTG
CTCAACAAGAACTGCCACGCCGGCACCCAGGTCTTCCTCTGCTCGCTCTTCGCGCCCGTC
TGCCTGGACCGGCCCATCTACCCGTGTCGCTGGCTCTGCGAGGCCGTGCGCGACTCGTGC
GAGCCGGTCATGCAGTTCTTCGGCTTCTACTGGCCCGAGATGCTTAAGTGTGACAAGTTC
CCCGAGGGGGACGTCTGCATCGCCATGACGCCGCCCAATGCCACCGAAGCCTCCAAGCCC
CAAGGCACAACGGTGTGTCCTCCCTGTGACAACGAGTTGAAATCTGAGGCCATCATTGAA
CATCTCTGTGCCAGCGAGTTTGCACTGAGGATGAAAATAAAAGAAGTGAAAAAAGAAAAT
GGCGACAAGAAGATTGTCCCCAAGAAGAAGAAGCCCCTGAAGTTGGGGCCCATCAAGAAG
AAGGACCTGAAGAAGCTTGTGCTGTACCTGAAGAATGGGGCTGACTGTCCCTGCCACCAG
CTGGACAACCTCAGCCACCACTTCCTCATCATGGGCCGCAAGGTGAAGAGCCAGTACTTG
CTGACGGCCATCCACAAGTGGGACAAGAAAAACAAGGAGTTCAAAAACTTCATGAAGAAA
ATGAAAAACCATGAGTGCCCCACCTTTCAGTCCGTGTTTAAGTTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1244
Start on reference sequence 303
Mutations:
None
Discrepancy:
ins@36,3;g321c,G107G;
g480c,P160P;
                                

5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 1015 1048
recombination site loxP site loxP recombination site 9 42
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : SFRP1
Symbol Nomenclature : SFRP1
SYNONYM : FRP; FRP-1; FRP1; FrzA; SARP2
Designation : SARP-2|sFRP-1|secreted apoptosis-related protein 2
Full Nomenclature : secreted frizzled-related protein 1
GENEID : 6422
GI : 61367355
GenBank Accession : AY891055
HGNC : 10776
MIM : 604156
Vega : OTTHUMG00000164074
Target GenBank: NM_003012

 

Reference Sequence Alignment

  

HsCD00022108      ATGGGCATCGGGCGCAGCGAGGGGGGCCGCCGCGGGGCAGCCCTGGGCGTGCTGCTGGCG
NM_003012.4       ATGGGCATCGGGCGCAGCGAGGGGGGCCGCCGCGGGGCAGCCCTGGGCGTGCTGCTGGCG
                  ************************************************************

HsCD00022108      CTGGGCGCGGCGCTTCTGGCCGTGGGCTCGGCCAGCGAGTACGACTACGTGAGCTTCCAG
NM_003012.4       CTGGGCGCGGCGCTTCTGGCCGTGGGCTCGGCCAGCGAGTACGACTACGTGAGCTTCCAG
                  ************************************************************

HsCD00022108      TCGGACATCGGCCCGTACCAGAGCGGGCGCTTCTACACCAAGCCACCTCAGTGCGTGGAC
NM_003012.4       TCGGACATCGGCCCGTACCAGAGCGGGCGCTTCTACACCAAGCCACCTCAGTGCGTGGAC
                  ************************************************************

HsCD00022108      ATCCCCGCGGACCTGCGGCTGTGCCACAACGTGGGCTACAAGAAGATGGTGCTGCCCAAC
NM_003012.4       ATCCCCGCGGACCTGCGGCTGTGCCACAACGTGGGCTACAAGAAGATGGTGCTGCCCAAC
                  ************************************************************

HsCD00022108      CTGCTGGAGCACGAGACCATGGCGGAGGTGAAGCAGCAGGCCAGCAGCTGGGTGCCCCTG
NM_003012.4       CTGCTGGAGCACGAGACCATGGCGGAGGTGAAGCAGCAGGCCAGCAGCTGGGTGCCCCTG
                  ************************************************************

HsCD00022108      CTCAACAAGAACTGCCACGCCGGCACCCAGGTCTTCCTCTGCTCGCTCTTCGCGCCCGTC
NM_003012.4       CTCAACAAGAACTGCCACGCCGGCACCCAGGTCTTCCTCTGCTCGCTCTTCGCGCCCGTC
                  ************************************************************

HsCD00022108      TGCCTGGACCGGCCCATCTACCCGTGTCGCTGGCTCTGCGAGGCCGTGCGCGACTCGTGC
NM_003012.4       TGCCTGGACCGGCCCATCTACCCGTGTCGCTGGCTCTGCGAGGCCGTGCGCGACTCGTGC
                  ************************************************************

HsCD00022108      GAGCCGGTCATGCAGTTCTTCGGCTTCTACTGGCCCGAGATGCTTAAGTGTGACAAGTTC
NM_003012.4       GAGCCGGTCATGCAGTTCTTCGGCTTCTACTGGCCCGAGATGCTTAAGTGTGACAAGTTC
                  ************************************************************

HsCD00022108      CCCGAGGGGGACGTCTGCATCGCCATGACGCCGCCCAATGCCACCGAAGCCTCCAAGCCC
NM_003012.4       CCCGAGGGGGACGTCTGCATCGCCATGACGCCGCCCAATGCCACCGAAGCCTCCAAGCCC
                  ************************************************************

HsCD00022108      CAAGGCACAACGGTGTGTCCTCCCTGTGACAACGAGTTGAAATCTGAGGCCATCATTGAA
NM_003012.4       CAAGGCACAACGGTGTGTCCTCCCTGTGACAACGAGTTGAAATCTGAGGCCATCATTGAA
                  ************************************************************

HsCD00022108      CATCTCTGTGCCAGCGAGTTTGCACTGAGGATGAAAATAAAAGAAGTGAAAAAAGAAAAT
NM_003012.4       CATCTCTGTGCCAGCGAGTTTGCACTGAGGATGAAAATAAAAGAAGTGAAAAAAGAAAAT
                  ************************************************************

HsCD00022108      GGCGACAAGAAGATTGTCCCCAAGAAGAAGAAGCCCCTGAAGTTGGGGCCCATCAAGAAG
NM_003012.4       GGCGACAAGAAGATTGTCCCCAAGAAGAAGAAGCCCCTGAAGTTGGGGCCCATCAAGAAG
                  ************************************************************

HsCD00022108      AAGGACCTGAAGAAGCTTGTGCTGTACCTGAAGAATGGGGCTGACTGTCCCTGCCACCAG
NM_003012.4       AAGGACCTGAAGAAGCTTGTGCTGTACCTGAAGAATGGGGCTGACTGTCCCTGCCACCAG
                  ************************************************************

HsCD00022108      CTGGACAACCTCAGCCACCACTTCCTCATCATGGGCCGCAAGGTGAAGAGCCAGTACTTG
NM_003012.4       CTGGACAACCTCAGCCACCACTTCCTCATCATGGGCCGCAAGGTGAAGAGCCAGTACTTG
                  ************************************************************

HsCD00022108      CTGACGGCCATCCACAAGTGGGACAAGAAAAACAAGGAGTTCAAAAACTTCATGAAGAAA
NM_003012.4       CTGACGGCCATCCACAAGTGGGACAAGAAAAACAAGGAGTTCAAAAACTTCATGAAGAAA
                  ************************************************************

HsCD00022108      ATGAAAAACCATGAGTGCCCCACCTTTCAGTCCGTGTTTAAGTTG
NM_003012.4       ATGAAAAACCATGAGTGCCCCACCTTTCAGTCCGTGTTTAAGTGA
                  ******************************************* .

Pathways

NCI : Validated targets of C-MYC transcriptional repression
Panther : Angiogenesis
Panther : Wnt signaling pathway
Reactome : Signal Transduction
Reactome : Signaling by Wnt
Reactome : TCF dependent signaling in response to WNT
Reactome : negative regulation of TCF-dependent signaling by WNT ligand antagonists

Protein

SMART domain : C345C : Netrin C-terminal Domain
SMART domain : FRI : Frizzled
UniProt : Q8N474
HPRD : 09165

Clone

Cloning Information : 19929
HIP Master Clone ID : 34439
Original Clone ID : FLH019929.01L

Organism


TAX_ID : 9606
Species Specific ID: 6422

Genome

Chromosome : 8
Map Location : 8p11.21
Ensembl : ENSG00000104332

Reagents

Labome : SFRP1-antibody