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NCKAP1 (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  NCKAP1
Gene Name:  NCK-associated protein 1
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH110727.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: PMID: 16512675
Title: Functional proteomics approach to investigate the biological activities of cDNAs implicated in breast cancer.
Authors: HIP
HsCD00002335

Price:  Login for Pricing
AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 3387nts         Open reading frame : 1 to 3387
Reference Sequence Alignment

ATGTCGCGCTCAGTGCTGCAGCCCAGTCAGCAGAAGCTGGCGGAGAAGCTCACCATCCTC
AACGACCGGGGCGTCGGCATGCTCACCCGCCTCTACAACATCAAGAAGGCATGTGGAGAC
CCCAAGGCAAAACCATCCTATCTTATCGACAAAAACCTGGAATCTGCTGTGAAATTCATA
GTCAGAAAATTCCCTGCTGTAGAAACCCGCAACAACAATCAACAGCTTGCACAACTACAG
AAAGAAAAATCAGAGATTCTGAAAAATCTGGCATTATATTACTTCACATTTGTAGATGTT
ATGGAATTTAAGGACCATGTTTGTGAATTGCTGAATACTATTGACGTTTGCCAAGTCTTC
TTTGATATTACTGTAAACTTTGATTTAACAAAGAACTACTTAGATTTAATTATAACCTAT
ACAACACTAATGATACTGCTGTCTCGAATTGAAGAAAGGAAGGCAATCATTGGATTATAC
AACTATGCCCATGAAATGACTCATGGAGCAAGTGACAGAGAATACCCACGCCTTGGCCAG
ATGATTGTGGATTATGAAAACCCTTTAAAGAAGATGATGGAAGAATTTGTACCCCATAGC
AAGTCTCTTTCAGATGCACTAATTTCTCTTCAAATGGTATATCCTCGAAGGAATCTTTCA
GCTGACCAGTGGAGAAATGCCCAGTTATTGAGCCTCATCAGTGCACCTAGTACAATGCTT
AATCCAGCACAGTCCGACACTATGCCTTGTGAATACCTCTCTTTGGATGCAATGGAAAAG
TGGATTATCTTTGGCTTTATTTTGTGCCATGGGATCCTAAATACTGACGCTACAGCACTG
AACCTTTGGAAACTAGCTCTTCAAAGTAGCTCTTGCCTCTCTCTCTTTCGGGATGAAGTT
TTCCACATTCACAAAGCTGCAGAAGACTTATTTGTAAACATACGAGGCTATAATAAACGT
ATTAATGACATAAGAGAATGCAAGGAGGCAGCCGTGTCACATGCTGGTTCAATGCACAGA
GAAAGACGCAAGTTTTTAAGATCTGCACTGAAGGAATTGGCTACTGTCCTCTCTGATCAA
CCTGGATTGCTAGGTCCCAAGGCACTTTTTGTTTTTATGGCATTATCCTTTGCCCGTGAT
GAAATCATCTGGCTACTTCGTCATGCAGATAACATGCCAAAGAAGAGTGCAGACGACTTT
ATAGATAAGCACATTGCTGAATTAATATTTTACATGGAAGAACTTAGAGCACATGTGAGG
AAATACGGACCTGTAATGCAGAGGTATTACGTGCAGTACCTTTCTGGCTTTGATGCTGTT
GTCCTCAATGAACTCGTGCAGAATCTTTCTGTTTGCCCTGAAGATGAATCAATCATCATG
TCCTCTTTTGTTAACACTATGACTTCCCTAAGTGTAAAACAAGTTGAAGATGGGGAAGTA
TTTGATTTCAGAGGAATGAGATTAGATTGGTTTAGGTTACAGGCATATACTAGTGTCTCA
AAGGCTTCACTTGGCCTTGCAGATCACAGAGAACTTGGAAAGATGATGAATACAATAATT
TTTCATACAAAAATGGTAGATTCCTTGGTGGAAATGTTGGTGGAAACATCAGATCTCTCC
ATATTTTGTTTTTATAGTCGTGCTTTTGAGAAGATGTTTCAACAGTGTTTGGAGTTACCC
TCTCAATCAAGATACTCAATTGCATTTCCACTACTTTGCACTCATTTTATGAGTTGCACG
CATGAACTATGTCCAGAAGAGCGACATCATATTGGAGATCGCAGTCTTTCCTTATGTAAT
ATGTTCCTAGATGAAATGGCCAAACAAGCTCGAAATCTCATCACTGATATTTGCACAGAA
CAGTGTACCCTTAGTGACCAGTTGCTACCCAAGCATTGTGCCAAAACTATCAGTCAAGCA
GTGAATAAGAAATCAAAAAAGCAGACTGGTAAGAAAGGGGAACCTGAAAGGGAGAAACCA
GGTGTTGAGAGCATGAGGAAAAACAGGCTGGTTGTGACCAACCTTGATAAATTGCACACT
GCACTTTCTGAGTTATGCTTCTCTATAAATTATGTACCAAACATGGTGGTATGGGAACAT
ACCTTTACCCCACGAGAATATTTGACTTCTCATCTGGAAATACGCTTTACCAAGTCAATT
GTTGGGATGACTATGTATAATCAAGCCACACAGGAAATTGCAAAACCTTCAGAACTTCTA
ACAAGTGTAAGAGCATACATGACCGTACTCCAGTCAATAGAAAACTATGTGCAGATTGAT
ATTACAAGAGTATTTAATAATGTGCTTCTTCAACAAACACAACATTTAGACAGTCATGGA
GAGCCAACCATTACAAGTCTATACACAAATTGGTATTTGGAAACTTTGTTACGACAAGTC
AGCAATGGCCATATAGCATATTTTCCTGCAATGAAAGCGTTTGTGAACTTACCTACAGAA
AATGAATTAACATTCAATGCAGAGGAATATTCTGACATATCAGAAATGAGGTCATTATCA
GAACTACTAGGCCCATATGGTATGAAGTTTCTAAGTGAAAGCCTTATGTGGCATATTTCA
TCACAAGTTGCTGAACTTAAGAAACTTGTGGTGGAGAATGTTGATGTGTTAACACAAATG
AGGACCAGCTTTGACAAACCAGACCAGATGGCTGCACTGTTTAAAAGATTATCATCTGTT
GACAGTGTCTTGAAGAGGATGACAATAATTGGTGTAATTTTATCCTTCCGATCATTGGCA
CAAGAAGCACTTAGAGATGTCTTATCCTACCACATTCCTTTTCTTGTAAGTTCAATTGAA
GATTTTAAGGATCACATTCCAAGGGAAACTGATATGAAGGTTGCAATGAATGTGTATGAG
TTATCATCAGCTGCCGGATTACCTTGTGAGATTGATCCTGCATTGGTCGTAGCTCTTTCT
TCACAAAAATCGGAAAACATTAGTCCAGAAGAAGAGTATAAAATTGCCTGCCTTCTCATG
GTGTTTGTGGCAGTTTCTTTGCCAACACTGGCCAGTAATGTGATGTCTCAGTACAGCCCT
GCTATAGAAGGGCATTGCAACAACATACATTGCTTGGCCAAAGCCATCAACCAGATTGCT
GCAGCTTTGTTTACAATTCACAAAGGAAGCATTGAAGACCGTCTTAAAGAATTTCTGGCG
CTTGCATCCTCCAGTCTACTGAAAATTGGCCAGGAGACAGATAAAACTACAACAAGAAAT
AGAGAATCTGTTTATTTACTGCTAGATATGATTGTACAAGAATCTCCATTCCTTACAATG
GATCTTTTGGAATCTTGTTTTCCTTATGTCTTGCTGAGAAATGCATACCATGCTGTCTAC
AAACAAAGTGTTACATCTTCTGCATAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 3658
Start on reference sequence 272
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 1015 1048
recombination site loxP site loxP recombination site 9 42
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : NCKAP1
Symbol Nomenclature : NCKAP1
SYNONYM : HEM2; NAP1; NAP125; p125Nap1
Designation : membrane-associated protein HEM-2|nck-associated protein 1
Full Nomenclature : NCK-associated protein 1
GENEID : 10787
GI : 61361047
GenBank Accession : AY889037
HGNC : 7666
MIM : 604891
Vega : OTTHUMG00000132623
Target GenBank: BC015025

 

Reference Sequence Alignment

  

HsCD00002335      ATGTCGCGCTCAGTGCTGCAGCCCAGTCAGCAGAAGCTGGCGGAGAAGCTCACCATCCTC
NM_205842.2       ATGTCGCGCTCAGTGCTGCAGCCCAGTCAGCAGAAGCTGGCGGAGAAGCTCACCATCCTC
NM_013436.4       ATGTCGCGCTCAGTGCTGCAGCCCAGTCAGCAGAAGCTGGCGGAGAAGCTCACCATCCTC
                  ************************************************************

HsCD00002335      AACGACCGGGGCGTCGGCATGCTCACCCGCCTCTACAACATCAAGAAGGCATG-------
NM_205842.2       AACGACCGGGGCGTCGGCATGCTCACCCGCCTCTACAACATCAAGAAGCAAGGACAAGTT
NM_013436.4       AACGACCGGGGCGTCGGCATGCTCACCCGCCTCTACAACATCAAGAAGGCAT--------
                  ************************************************ .*         

HsCD00002335      -----------TGGAGACCCCAAGGCAAAACCATCCTATCTTATCGACAAAAACCTGGAA
NM_205842.2       TGGAAGGCATGTGGAGACCCCAAGGCAAAACCATCCTATCTTATCGACAAAAACCTGGAA
NM_013436.4       ----------GTGGAGACCCCAAGGCAAAACCATCCTATCTTATCGACAAAAACCTGGAA
                             *************************************************

HsCD00002335      TCTGCTGTGAAATTCATAGTCAGAAAATTCCCTGCTGTAGAAACCCGCAACAACAATCAA
NM_205842.2       TCTGCTGTGAAATTCATAGTCAGAAAATTCCCTGCTGTAGAAACCCGCAACAACAATCAA
NM_013436.4       TCTGCTGTGAAATTCATAGTCAGAAAATTCCCTGCTGTAGAAACCCGCAACAACAATCAA
                  ************************************************************

HsCD00002335      CAGCTTGCACAACTACAGAAAGAAAAATCAGAGATTCTGAAAAATCTGGCATTATATTAC
NM_205842.2       CAGCTTGCACAACTACAGAAAGAAAAATCAGAGATTCTGAAAAATCTGGCATTATATTAC
NM_013436.4       CAGCTTGCACAACTACAGAAAGAAAAATCAGAGATTCTGAAAAATCTGGCATTATATTAC
                  ************************************************************

HsCD00002335      TTCACATTTGTAGATGTTATGGAATTTAAGGACCATGTTTGTGAATTGCTGAATACTATT
NM_205842.2       TTCACATTTGTAGATGTTATGGAATTTAAGGACCATGTTTGTGAATTGCTGAATACTATT
NM_013436.4       TTCACATTTGTAGATGTTATGGAATTTAAGGACCATGTTTGTGAATTGCTGAATACTATT
                  ************************************************************

HsCD00002335      GACGTTTGCCAAGTCTTCTTTGATATTACTGTAAACTTTGATTTAACAAAGAACTACTTA
NM_205842.2       GACGTTTGCCAAGTCTTCTTTGATATTACTGTAAACTTTGATTTAACAAAGAACTACTTA
NM_013436.4       GACGTTTGCCAAGTCTTCTTTGATATTACTGTAAACTTTGATTTAACAAAGAACTACTTA
                  ************************************************************

HsCD00002335      GATTTAATTATAACCTATACAACACTAATGATACTGCTGTCTCGAATTGAAGAAAGGAAG
NM_205842.2       GATTTAATTATAACCTATACAACACTAATGATACTGCTGTCTCGAATTGAAGAAAGGAAG
NM_013436.4       GATTTAATTATAACCTATACAACACTAATGATACTGCTGTCTCGAATTGAAGAAAGGAAG
                  ************************************************************

HsCD00002335      GCAATCATTGGATTATACAACTATGCCCATGAAATGACTCATGGAGCAAGTGACAGAGAA
NM_205842.2       GCAATCATTGGATTATACAACTATGCCCATGAAATGACTCATGGAGCAAGTGACAGAGAA
NM_013436.4       GCAATCATTGGATTATACAACTATGCCCATGAAATGACTCATGGAGCAAGTGACAGAGAA
                  ************************************************************

HsCD00002335      TACCCACGCCTTGGCCAGATGATTGTGGATTATGAAAACCCTTTAAAGAAGATGATGGAA
NM_205842.2       TACCCACGCCTTGGCCAGATGATTGTGGATTATGAAAACCCTTTAAAGAAGATGATGGAA
NM_013436.4       TACCCACGCCTTGGCCAGATGATTGTGGATTATGAAAACCCTTTAAAGAAGATGATGGAA
                  ************************************************************

HsCD00002335      GAATTTGTACCCCATAGCAAGTCTCTTTCAGATGCACTAATTTCTCTTCAAATGGTATAT
NM_205842.2       GAATTTGTACCCCATAGCAAGTCTCTTTCAGATGCACTAATTTCTCTTCAAATGGTATAT
NM_013436.4       GAATTTGTACCCCATAGCAAGTCTCTTTCAGATGCACTAATTTCTCTTCAAATGGTATAT
                  ************************************************************

HsCD00002335      CCTCGAAGGAATCTTTCAGCTGACCAGTGGAGAAATGCCCAGTTATTGAGCCTCATCAGT
NM_205842.2       CCTCGAAGGAATCTTTCAGCTGACCAGTGGAGAAATGCCCAGTTATTGAGCCTCATCAGT
NM_013436.4       CCTCGAAGGAATCTTTCAGCTGACCAGTGGAGAAATGCCCAGTTATTGAGCCTCATCAGT
                  ************************************************************

HsCD00002335      GCACCTAGTACAATGCTTAATCCAGCACAGTCCGACACTATGCCTTGTGAATACCTCTCT
NM_205842.2       GCACCTAGTACAATGCTTAATCCAGCACAGTCCGACACTATGCCTTGTGAATACCTCTCT
NM_013436.4       GCACCTAGTACAATGCTTAATCCAGCACAGTCCGACACTATGCCTTGTGAATACCTCTCT
                  ************************************************************

HsCD00002335      TTGGATGCAATGGAAAAGTGGATTATCTTTGGCTTTATTTTGTGCCATGGGATCCTAAAT
NM_205842.2       TTGGATGCAATGGAAAAGTGGATTATCTTTGGCTTTATTTTGTGCCATGGGATCCTAAAT
NM_013436.4       TTGGATGCAATGGAAAAGTGGATTATCTTTGGCTTTATTTTGTGCCATGGGATCCTAAAT
                  ************************************************************

HsCD00002335      ACTGACGCTACAGCACTGAACCTTTGGAAACTAGCTCTTCAAAGTAGCTCTTGCCTCTCT
NM_205842.2       ACTGACGCTACAGCACTGAACCTTTGGAAACTAGCTCTTCAAAGTAGCTCTTGCCTCTCT
NM_013436.4       ACTGACGCTACAGCACTGAACCTTTGGAAACTAGCTCTTCAAAGTAGCTCTTGCCTCTCT
                  ************************************************************

HsCD00002335      CTCTTTCGGGATGAAGTTTTCCACATTCACAAAGCTGCAGAAGACTTATTTGTAAACATA
NM_205842.2       CTCTTTCGGGATGAAGTTTTCCACATTCACAAAGCTGCAGAAGACTTATTTGTAAACATA
NM_013436.4       CTCTTTCGGGATGAAGTTTTCCACATTCACAAAGCTGCAGAAGACTTATTTGTAAACATA
                  ************************************************************

HsCD00002335      CGAGGCTATAATAAACGTATTAATGACATAAGAGAATGCAAGGAGGCAGCCGTGTCACAT
NM_205842.2       CGAGGCTATAATAAACGTATTAATGACATAAGAGAATGCAAGGAGGCAGCCGTGTCACAT
NM_013436.4       CGAGGCTATAATAAACGTATTAATGACATAAGAGAATGCAAGGAGGCAGCCGTGTCACAT
                  ************************************************************

HsCD00002335      GCTGGTTCAATGCACAGAGAAAGACGCAAGTTTTTAAGATCTGCACTGAAGGAATTGGCT
NM_205842.2       GCTGGTTCAATGCACAGAGAAAGACGCAAGTTTTTAAGATCTGCACTGAAGGAATTGGCT
NM_013436.4       GCTGGTTCAATGCACAGAGAAAGACGCAAGTTTTTAAGATCTGCACTGAAGGAATTGGCT
                  ************************************************************

HsCD00002335      ACTGTCCTCTCTGATCAACCTGGATTGCTAGGTCCCAAGGCACTTTTTGTTTTTATGGCA
NM_205842.2       ACTGTCCTCTCTGATCAACCTGGATTGCTAGGTCCCAAGGCACTTTTTGTTTTTATGGCA
NM_013436.4       ACTGTCCTCTCTGATCAACCTGGATTGCTAGGTCCCAAGGCACTTTTTGTTTTTATGGCA
                  ************************************************************

HsCD00002335      TTATCCTTTGCCCGTGATGAAATCATCTGGCTACTTCGTCATGCAGATAACATGCCAAAG
NM_205842.2       TTATCCTTTGCCCGTGATGAAATCATCTGGCTACTTCGTCATGCAGATAACATGCCAAAG
NM_013436.4       TTATCCTTTGCCCGTGATGAAATCATCTGGCTACTTCGTCATGCAGATAACATGCCAAAG
                  ************************************************************

HsCD00002335      AAGAGTGCAGACGACTTTATAGATAAGCACATTGCTGAATTAATATTTTACATGGAAGAA
NM_205842.2       AAGAGTGCAGACGACTTTATAGATAAGCACATTGCTGAATTAATATTTTACATGGAAGAA
NM_013436.4       AAGAGTGCAGACGACTTTATAGATAAGCACATTGCTGAATTAATATTTTACATGGAAGAA
                  ************************************************************

HsCD00002335      CTTAGAGCACATGTGAGGAAATACGGACCTGTAATGCAGAGGTATTACGTGCAGTACCTT
NM_205842.2       CTTAGAGCACATGTGAGGAAATACGGACCTGTAATGCAGAGGTATTACGTGCAGTACCTT
NM_013436.4       CTTAGAGCACATGTGAGGAAATACGGACCTGTAATGCAGAGGTATTACGTGCAGTACCTT
                  ************************************************************

HsCD00002335      TCTGGCTTTGATGCTGTTGTCCTCAATGAACTCGTGCAGAATCTTTCTGTTTGCCCTGAA
NM_205842.2       TCTGGCTTTGATGCTGTTGTCCTCAATGAACTCGTGCAGAATCTTTCTGTTTGCCCTGAA
NM_013436.4       TCTGGCTTTGATGCTGTTGTCCTCAATGAACTCGTGCAGAATCTTTCTGTTTGCCCTGAA
                  ************************************************************

HsCD00002335      GATGAATCAATCATCATGTCCTCTTTTGTTAACACTATGACTTCCCTAAGTGTAAAACAA
NM_205842.2       GATGAATCAATCATCATGTCCTCTTTTGTTAACACTATGACTTCCCTAAGTGTAAAACAA
NM_013436.4       GATGAATCAATCATCATGTCCTCTTTTGTTAACACTATGACTTCCCTAAGTGTAAAACAA
                  ************************************************************

HsCD00002335      GTTGAAGATGGGGAAGTATTTGATTTCAGAGGAATGAGATTAGATTGGTTTAGGTTACAG
NM_205842.2       GTTGAAGATGGGGAAGTATTTGATTTCAGAGGAATGAGATTAGATTGGTTTAGGTTACAG
NM_013436.4       GTTGAAGATGGGGAAGTATTTGATTTCAGAGGAATGAGATTAGATTGGTTTAGGTTACAG
                  ************************************************************

HsCD00002335      GCATATACTAGTGTCTCAAAGGCTTCACTTGGCCTTGCAGATCACAGAGAACTTGGAAAG
NM_205842.2       GCATATACTAGTGTCTCAAAGGCTTCACTTGGCCTTGCAGATCACAGAGAACTTGGAAAG
NM_013436.4       GCATATACTAGTGTCTCAAAGGCTTCACTTGGCCTTGCAGATCACAGAGAACTTGGAAAG
                  ************************************************************

HsCD00002335      ATGATGAATACAATAATTTTTCATACAAAAATGGTAGATTCCTTGGTGGAAATGTTGGTG
NM_205842.2       ATGATGAATACAATAATTTTTCATACAAAAATGGTAGATTCCTTGGTGGAAATGTTGGTG
NM_013436.4       ATGATGAATACAATAATTTTTCATACAAAAATGGTAGATTCCTTGGTGGAAATGTTGGTG
                  ************************************************************

HsCD00002335      GAAACATCAGATCTCTCCATATTTTGTTTTTATAGTCGTGCTTTTGAGAAGATGTTTCAA
NM_205842.2       GAAACATCAGATCTCTCCATATTTTGTTTTTATAGTCGTGCTTTTGAGAAGATGTTTCAA
NM_013436.4       GAAACATCAGATCTCTCCATATTTTGTTTTTATAGTCGTGCTTTTGAGAAGATGTTTCAA
                  ************************************************************

HsCD00002335      CAGTGTTTGGAGTTACCCTCTCAATCAAGATACTCAATTGCATTTCCACTACTTTGCACT
NM_205842.2       CAGTGTTTGGAGTTACCCTCTCAATCAAGATACTCAATTGCATTTCCACTACTTTGCACT
NM_013436.4       CAGTGTTTGGAGTTACCCTCTCAATCAAGATACTCAATTGCATTTCCACTACTTTGCACT
                  ************************************************************

HsCD00002335      CATTTTATGAGTTGCACGCATGAACTATGTCCAGAAGAGCGACATCATATTGGAGATCGC
NM_205842.2       CATTTTATGAGTTGCACGCATGAACTATGTCCAGAAGAGCGACATCATATTGGAGATCGC
NM_013436.4       CATTTTATGAGTTGCACGCATGAACTATGTCCAGAAGAGCGACATCATATTGGAGATCGC
                  ************************************************************

HsCD00002335      AGTCTTTCCTTATGTAATATGTTCCTAGATGAAATGGCCAAACAAGCTCGAAATCTCATC
NM_205842.2       AGTCTTTCCTTATGTAATATGTTCCTAGATGAAATGGCCAAACAAGCTCGAAATCTCATC
NM_013436.4       AGTCTTTCCTTATGTAATATGTTCCTAGATGAAATGGCCAAACAAGCTCGAAATCTCATC
                  ************************************************************

HsCD00002335      ACTGATATTTGCACAGAACAGTGTACCCTTAGTGACCAGTTGCTACCCAAGCATTGTGCC
NM_205842.2       ACTGATATTTGCACAGAACAGTGTACCCTTAGTGACCAGTTGCTACCCAAGCATTGTGCC
NM_013436.4       ACTGATATTTGCACAGAACAGTGTACCCTTAGTGACCAGTTGCTACCCAAGCATTGTGCC
                  ************************************************************

HsCD00002335      AAAACTATCAGTCAAGCAGTGAATAAGAAATCAAAAAAGCAGACTGGTAAGAAAGGGGAA
NM_205842.2       AAAACTATCAGTCAAGCAGTGAATAAGAAATCAAAAAAGCAGACTGGTAAGAAAGGGGAA
NM_013436.4       AAAACTATCAGTCAAGCAGTGAATAAGAAATCAAAAAAGCAGACTGGTAAGAAAGGGGAA
                  ************************************************************

HsCD00002335      CCTGAAAGGGAGAAACCAGGTGTTGAGAGCATGAGGAAAAACAGGCTGGTTGTGACCAAC
NM_205842.2       CCTGAAAGGGAGAAACCAGGTGTTGAGAGCATGAGGAAAAACAGGCTGGTTGTGACCAAC
NM_013436.4       CCTGAAAGGGAGAAACCAGGTGTTGAGAGCATGAGGAAAAACAGGCTGGTTGTGACCAAC
                  ************************************************************

HsCD00002335      CTTGATAAATTGCACACTGCACTTTCTGAGTTATGCTTCTCTATAAATTATGTACCAAAC
NM_205842.2       CTTGATAAATTGCACACTGCACTTTCTGAGTTATGCTTCTCTATAAATTATGTACCAAAC
NM_013436.4       CTTGATAAATTGCACACTGCACTTTCTGAGTTATGCTTCTCTATAAATTATGTACCAAAC
                  ************************************************************

HsCD00002335      ATGGTGGTATGGGAACATACCTTTACCCCACGAGAATATTTGACTTCTCATCTGGAAATA
NM_205842.2       ATGGTGGTATGGGAACATACCTTTACCCCACGAGAATATTTGACTTCTCATCTGGAAATA
NM_013436.4       ATGGTGGTATGGGAACATACCTTTACCCCACGAGAATATTTGACTTCTCATCTGGAAATA
                  ************************************************************

HsCD00002335      CGCTTTACCAAGTCAATTGTTGGGATGACTATGTATAATCAAGCCACACAGGAAATTGCA
NM_205842.2       CGCTTTACCAAGTCAATTGTTGGGATGACTATGTATAATCAAGCCACACAGGAAATTGCA
NM_013436.4       CGCTTTACCAAGTCAATTGTTGGGATGACTATGTATAATCAAGCCACACAGGAAATTGCA
                  ************************************************************

HsCD00002335      AAACCTTCAGAACTTCTAACAAGTGTAAGAGCATACATGACCGTACTCCAGTCAATAGAA
NM_205842.2       AAACCTTCAGAACTTCTAACAAGTGTAAGAGCATACATGACCGTACTCCAGTCAATAGAA
NM_013436.4       AAACCTTCAGAACTTCTAACAAGTGTAAGAGCATACATGACCGTACTCCAGTCAATAGAA
                  ************************************************************

HsCD00002335      AACTATGTGCAGATTGATATTACAAGAGTATTTAATAATGTGCTTCTTCAACAAACACAA
NM_205842.2       AACTATGTGCAGATTGATATTACAAGAGTATTTAATAATGTGCTTCTTCAACAAACACAA
NM_013436.4       AACTATGTGCAGATTGATATTACAAGAGTATTTAATAATGTGCTTCTTCAACAAACACAA
                  ************************************************************

HsCD00002335      CATTTAGACAGTCATGGAGAGCCAACCATTACAAGTCTATACACAAATTGGTATTTGGAA
NM_205842.2       CATTTAGACAGTCATGGAGAGCCAACCATTACAAGTCTATACACAAATTGGTATTTGGAA
NM_013436.4       CATTTAGACAGTCATGGAGAGCCAACCATTACAAGTCTATACACAAATTGGTATTTGGAA
                  ************************************************************

HsCD00002335      ACTTTGTTACGACAAGTCAGCAATGGCCATATAGCATATTTTCCTGCAATGAAAGCGTTT
NM_205842.2       ACTTTGTTACGACAAGTCAGCAATGGCCATATAGCATATTTTCCTGCAATGAAAGCGTTT
NM_013436.4       ACTTTGTTACGACAAGTCAGCAATGGCCATATAGCATATTTTCCTGCAATGAAAGCGTTT
                  ************************************************************

HsCD00002335      GTGAACTTACCTACAGAAAATGAATTAACATTCAATGCAGAGGAATATTCTGACATATCA
NM_205842.2       GTGAACTTACCTACAGAAAATGAATTAACATTCAATGCAGAGGAATATTCTGACATATCA
NM_013436.4       GTGAACTTACCTACAGAAAATGAATTAACATTCAATGCAGAGGAATATTCTGACATATCA
                  ************************************************************

HsCD00002335      GAAATGAGGTCATTATCAGAACTACTAGGCCCATATGGTATGAAGTTTCTAAGTGAAAGC
NM_205842.2       GAAATGAGGTCATTATCAGAACTACTAGGCCCATATGGTATGAAGTTTCTAAGTGAAAGC
NM_013436.4       GAAATGAGGTCATTATCAGAACTACTAGGCCCATATGGTATGAAGTTTCTAAGTGAAAGC
                  ************************************************************

HsCD00002335      CTTATGTGGCATATTTCATCACAAGTTGCTGAACTTAAGAAACTTGTGGTGGAGAATGTT
NM_205842.2       CTTATGTGGCATATTTCATCACAAGTTGCTGAACTTAAGAAACTTGTGGTGGAGAATGTT
NM_013436.4       CTTATGTGGCATATTTCATCACAAGTTGCTGAACTTAAGAAACTTGTGGTGGAGAATGTT
                  ************************************************************

HsCD00002335      GATGTGTTAACACAAATGAGGACCAGCTTTGACAAACCAGACCAGATGGCTGCACTGTTT
NM_205842.2       GATGTGTTAACACAAATGAGGACCAGCTTTGACAAACCAGACCAGATGGCTGCACTGTTT
NM_013436.4       GATGTGTTAACACAAATGAGGACCAGCTTTGACAAACCAGACCAGATGGCTGCACTGTTT
                  ************************************************************

HsCD00002335      AAAAGATTATCATCTGTTGACAGTGTCTTGAAGAGGATGACAATAATTGGTGTAATTTTA
NM_205842.2       AAAAGATTATCATCTGTTGACAGTGTCTTGAAGAGGATGACAATAATTGGTGTAATTTTA
NM_013436.4       AAAAGATTATCATCTGTTGACAGTGTCTTGAAGAGGATGACAATAATTGGTGTAATTTTA
                  ************************************************************

HsCD00002335      TCCTTCCGATCATTGGCACAAGAAGCACTTAGAGATGTCTTATCCTACCACATTCCTTTT
NM_205842.2       TCCTTCCGATCATTGGCACAAGAAGCACTTAGAGATGTCTTATCCTACCACATTCCTTTT
NM_013436.4       TCCTTCCGATCATTGGCACAAGAAGCACTTAGAGATGTCTTATCCTACCACATTCCTTTT
                  ************************************************************

HsCD00002335      CTTGTAAGTTCAATTGAAGATTTTAAGGATCACATTCCAAGGGAAACTGATATGAAGGTT
NM_205842.2       CTTGTAAGTTCAATTGAAGATTTTAAGGATCACATTCCAAGGGAAACTGATATGAAGGTT
NM_013436.4       CTTGTAAGTTCAATTGAAGATTTTAAGGATCACATTCCAAGGGAAACTGATATGAAGGTT
                  ************************************************************

HsCD00002335      GCAATGAATGTGTATGAGTTATCATCAGCTGCCGGATTACCTTGTGAGATTGATCCTGCA
NM_205842.2       GCAATGAATGTGTATGAGTTATCATCAGCTGCCGGATTACCTTGTGAGATTGATCCTGCA
NM_013436.4       GCAATGAATGTGTATGAGTTATCATCAGCTGCCGGATTACCTTGTGAGATTGATCCTGCA
                  ************************************************************

HsCD00002335      TTGGTCGTAGCTCTTTCTTCACAAAAATCGGAAAACATTAGTCCAGAAGAAGAGTATAAA
NM_205842.2       TTGGTCGTAGCTCTTTCTTCACAAAAATCGGAAAACATTAGTCCAGAAGAAGAGTATAAA
NM_013436.4       TTGGTCGTAGCTCTTTCTTCACAAAAATCGGAAAACATTAGTCCAGAAGAAGAGTATAAA
                  ************************************************************

HsCD00002335      ATTGCCTGCCTTCTCATGGTGTTTGTGGCAGTTTCTTTGCCAACACTGGCCAGTAATGTG
NM_205842.2       ATTGCCTGCCTTCTCATGGTGTTTGTGGCAGTTTCTTTGCCAACACTGGCCAGTAATGTG
NM_013436.4       ATTGCCTGCCTTCTCATGGTGTTTGTGGCAGTTTCTTTGCCAACACTGGCCAGTAATGTG
                  ************************************************************

HsCD00002335      ATGTCTCAGTACAGCCCTGCTATAGAAGGGCATTGCAACAACATACATTGCTTGGCCAAA
NM_205842.2       ATGTCTCAGTACAGCCCTGCTATAGAAGGGCATTGCAACAACATACATTGCTTGGCCAAA
NM_013436.4       ATGTCTCAGTACAGCCCTGCTATAGAAGGGCATTGCAACAACATACATTGCTTGGCCAAA
                  ************************************************************

HsCD00002335      GCCATCAACCAGATTGCTGCAGCTTTGTTTACAATTCACAAAGGAAGCATTGAAGACCGT
NM_205842.2       GCCATCAACCAGATTGCTGCAGCTTTGTTTACAATTCACAAAGGAAGCATTGAAGACCGT
NM_013436.4       GCCATCAACCAGATTGCTGCAGCTTTGTTTACAATTCACAAAGGAAGCATTGAAGACCGT
                  ************************************************************

HsCD00002335      CTTAAAGAATTTCTGGCGCTTGCATCCTCCAGTCTACTGAAAATTGGCCAGGAGACAGAT
NM_205842.2       CTTAAAGAATTTCTGGCGCTTGCATCCTCCAGTCTACTGAAAATTGGCCAGGAGACAGAT
NM_013436.4       CTTAAAGAATTTCTGGCGCTTGCATCCTCCAGTCTACTGAAAATTGGCCAGGAGACAGAT
                  ************************************************************

HsCD00002335      AAAACTACAACAAGAAATAGAGAATCTGTTTATTTACTGCTAGATATGATTGTACAAGAA
NM_205842.2       AAAACTACAACAAGAAATAGAGAATCTGTTTATTTACTGCTAGATATGATTGTACAAGAA
NM_013436.4       AAAACTACAACAAGAAATAGAGAATCTGTTTATTTACTGCTAGATATGATTGTACAAGAA
                  ************************************************************

HsCD00002335      TCTCCATTCCTTACAATGGATCTTTTGGAATCTTGTTTTCCTTATGTCTTGCTGAGAAAT
NM_205842.2       TCTCCATTCCTTACAATGGATCTTTTGGAATCTTGTTTTCCTTATGTCTTGCTGAGAAAT
NM_013436.4       TCTCCATTCCTTACAATGGATCTTTTGGAATCTTGTTTTCCTTATGTCTTGCTGAGAAAT
                  ************************************************************

HsCD00002335      GCATACCATGCTGTCTACAAACAAAGTGTTACATCTTCTGCATAG
NM_205842.2       GCATACCATGCTGTCTACAAACAAAGTGTTACATCTTCTGCATAA
NM_013436.4       GCATACCATGCTGTCTACAAACAAAGTGTTACATCTTCTGCATAA
                  ********************************************.

Pathways

NCI : E-cadherin signaling in the nascent adherens junction
NCI : ErbB1 downstream signaling
NCI : PDGFR-beta signaling pathway
NCI : RAC1 signaling pathway
NCI : Stabilization and expansion of the E-cadherin adherens junction
Reactome : Fcgamma receptor (FCGR) dependent phagocytosis
Reactome : Immune System
Reactome : Innate Immune System
Reactome : Regulation of actin dynamics for phagocytic cup formation
WikiPathway : Diurnally regulated genes with circadian orthologs
WikiPathway : Regulation of Actin Cytoskeleton

Protein

UniProt : Q9Y2A7
HPRD : 05353

Clone

Cloning Information : 110727
HIP Master Clone ID : 87010
Original Clone ID : FLH110727.01X

Organism


TAX_ID : 9606
Species Specific ID: 10787

Genome

Chromosome : 2
Map Location : 2q32
Ensembl : ENSG00000061676

Reagents

Labome : NCKAP1-antibody