DNASU Plasmid Repository • 480.965.5697 | Email

SNAP29 (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

TOP

Explanation of Terms


Gene: Gene Symbol:  SNAP29
Gene Name:  synaptosomal-associated protein, 29kDa
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH025491.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00002374

Price:  Login for Pricing
AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 777nts         Open reading frame : 1 to 777
Reference Sequence Alignment

ATGTCAGCTTACCCTAAGAGCTACAATCCGTTCGACGACGACGGGGAGGACGAAGGCGCC
CGGCCGGCCCCTTGGAGGGACGCCCGAGACCTCCCCGACGGGCCCGACGCGCCCGCGGAC
AGGCAGCAGTACTTGCGGCAGGAGGTCCTCCGCAGGGCTGAGGCCACGGCCGCCAGCACC
AGCAGGTCCCTGGCCCTCATGTACGAGTCCGAGAAGGTTGGGGTCGCCTCTTCCGAGGAG
CTCGCCCGTCAGCGAGGAGTCCTGGAGCGCACAGAGAAGATGGTGGACAAGATGGACCAA
GATTTGAAGATCAGCCAGAAACACATCAATAGCATTAAGAGCGTGTTTGGGGGGCTGGTC
AATTACTTCAAATCCAAACCAGTAGAGACCCCACCTGAACAGAATGGCACCCTCACCTCC
CAGCCCAACAACAGATTGAAAGAAGCTATAAGTACAAGTAAAGAACAGGAAGCAAAGTAC
CAGGCCAGCCACCCAAACCTTAGAAAGCTGGATGATACAGACCCTGTCCCCAGAGGGGCT
GGTTCTGCCATGAGTACTGATGCTTACCCAAAGAACCCACACCTTCGAGCCTATCACCAG
AAGATCGACAGCAACCTAGATGAGCTGTCCATGGGACTGGGTCGTCTGAAGGACATAGCC
CTGGGGATGCAGACAGAAATTGAGGAGCAAGATGACATTCTTGACCGGCTGACAACCAAA
GTGGACAAGTTAGATGTCAACATAAAAAGCACAGAAAGAAAAGTTCGACAACTCTTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 848
Start on reference sequence 72
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 1015 1048
recombination site loxP site loxP recombination site 9 42
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : SNAP29
Symbol Nomenclature : SNAP29
SYNONYM : CEDNIK; SNAP-29
Designation : soluble 29 kDa NSF attachment protein|synaptosomal-associated protein 29|vesicle-membrane fusion protein SNAP-29
Full Nomenclature : synaptosomal-associated protein, 29kDa
GENEID : 9342
GI : 61370879
GenBank Accession : AY891641
HGNC : 11133
MIM : 604202
Vega : OTTHUMG00000150765
Target GenBank: BC009715

 

Reference Sequence Alignment

  

HsCD00002374      ATGTCAGCTTACCCTAAGAGCTACAATCCGTTCGACGACGACGGGGAGGACGAAGGCGCC
NM_004782.3       ATGTCAGCTTACCCTAAAAGCTACAATCCGTTCGACGACGACGGGGAGGACGAAGGCGCC
                  *****************.******************************************

HsCD00002374      CGGCCGGCCCCTTGGAGGGACGCCCGAGACCTCCCCGACGGGCCCGACGCGCCCGCGGAC
NM_004782.3       CGGCCGGCCCCTTGGAGGGACGCCCGAGACCTCCCCGACGGGCCCGACGCGCCCGCGGAC
                  ************************************************************

HsCD00002374      AGGCAGCAGTACTTGCGGCAGGAGGTCCTCCGCAGGGCTGAGGCCACGGCCGCCAGCACC
NM_004782.3       AGGCAGCAGTACTTGCGGCAGGAGGTCCTCCGCAGGGCTGAGGCCACGGCCGCCAGCACC
                  ************************************************************

HsCD00002374      AGCAGGTCCCTGGCCCTCATGTACGAGTCCGAGAAGGTTGGGGTCGCCTCTTCCGAGGAG
NM_004782.3       AGCAGGTCCCTGGCCCTCATGTACGAGTCCGAGAAGGTTGGGGTCGCCTCTTCCGAGGAG
                  ************************************************************

HsCD00002374      CTCGCCCGTCAGCGAGGAGTCCTGGAGCGCACAGAGAAGATGGTGGACAAGATGGACCAA
NM_004782.3       CTCGCCCGTCAGCGAGGAGTCCTGGAGCGCACAGAGAAGATGGTGGACAAGATGGACCAA
                  ************************************************************

HsCD00002374      GATTTGAAGATCAGCCAGAAACACATCAATAGCATTAAGAGCGTGTTTGGGGGGCTGGTC
NM_004782.3       GATTTGAAGATCAGCCAGAAACACATCAATAGCATTAAGAGCGTGTTTGGGGGGCTGGTC
                  ************************************************************

HsCD00002374      AATTACTTCAAATCCAAACCAGTAGAGACCCCACCTGAACAGAATGGCACCCTCACCTCC
NM_004782.3       AATTACTTCAAATCCAAACCAGTAGAGACCCCACCTGAACAGAATGGCACCCTCACCTCC
                  ************************************************************

HsCD00002374      CAGCCCAACAACAGATTGAAAGAAGCTATAAGTACAAGTAAAGAACAGGAAGCAAAGTAC
NM_004782.3       CAGCCCAACAACAGATTGAAAGAAGCTATAAGTACAAGTAAAGAACAGGAAGCAAAGTAC
                  ************************************************************

HsCD00002374      CAGGCCAGCCACCCAAACCTTAGAAAGCTGGATGATACAGACCCTGTCCCCAGAGGGGCT
NM_004782.3       CAGGCCAGCCACCCAAACCTTAGAAAGCTGGATGATACAGACCCTGTCCCCAGAGGGGCT
                  ************************************************************

HsCD00002374      GGTTCTGCCATGAGTACTGATGCTTACCCAAAGAACCCACACCTTCGAGCCTATCACCAG
NM_004782.3       GGTTCTGCCATGAGTACTGATGCTTACCCAAAGAACCCACACCTTCGAGCCTATCACCAG
                  ************************************************************

HsCD00002374      AAGATCGACAGCAACCTAGATGAGCTGTCCATGGGACTGGGTCGTCTGAAGGACATAGCC
NM_004782.3       AAGATCGACAGCAACCTAGATGAGCTGTCCATGGGACTGGGTCGTCTGAAGGACATAGCC
                  ************************************************************

HsCD00002374      CTGGGGATGCAGACAGAAATTGAGGAGCAAGATGACATTCTTGACCGGCTGACAACCAAA
NM_004782.3       CTGGGGATGCAGACAGAAATTGAGGAGCAAGATGACATTCTTGACCGGCTGACAACCAAA
                  ************************************************************

HsCD00002374      GTGGACAAGTTAGATGTCAACATAAAAAGCACAGAAAGAAAAGTTCGACAACTCTTG
NM_004782.3       GTGGACAAGTTAGATGTCAACATAAAAAGCACAGAAAGAAAAGTTCGACAACTCTGA
                  ******************************************************* .

Pathways

Panther : 5HT1 type receptor mediated signaling pathway
Panther : 5HT2 type receptor mediated signaling pathway
Panther : 5HT3 type receptor mediated signaling pathway
Panther : 5HT4 type receptor mediated signaling pathway
Panther : Adrenaline and noradrenaline biosynthesis
Panther : Beta1 adrenergic receptor signaling pathway
Panther : Beta2 adrenergic receptor signaling pathway
Panther : Beta3 adrenergic receptor signaling pathway
Panther : Cortocotropin releasing factor receptor signaling pathway
Panther : Dopamine receptor mediated signaling pathway
Panther : Ionotropic glutamate receptor pathway
Panther : Metabotropic glutamate receptor group II pathway
Panther : Metabotropic glutamate receptor group III pathway
Panther : Muscarinic acetylcholine receptor 1 and 3 signaling pathway
Panther : Muscarinic acetylcholine receptor 2 and 4 signaling pathway
Panther : Nicotinic acetylcholine receptor signaling pathway
Panther : Opioid prodynorphin pathway
Panther : Opioid proenkephalin pathway
Panther : Opioid proopiomelanocortin pathway
Panther : Oxytocin receptor mediated signaling pathway
Panther : Thyrotropin-releasing hormone receptor signaling pathway
WikiPathway : Epithelium TarBase
WikiPathway : Lymphocyte TarBase
WikiPathway : Muscle cell TarBase

Protein

SMART domain : t_SNARE : Helical region found in SNAREs
UniProt : O95721
HPRD : 11968

Clone

Cloning Information : 25491
HIP Master Clone ID : 55867
Original Clone ID : FLH025491.01L

Organism


TAX_ID : 9606
Species Specific ID: 9342

Genome

Chromosome : 22
Map Location : 22q11.21
Ensembl : ENSG00000099940

Reagents

Labome : SNAP29-antibody