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TSG101 (Homo sapiens) in pDONR201 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  TSG101
Gene Name:  tumor susceptibility 101
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH056692.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR201               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00000245

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1173nts         Open reading frame : 1 to 1173
Reference Sequence Alignment

ATGGCGGTGTCGGAGAGCCAGCTCAAGAAAATGGTGTCCAAGTACAAATACAGAGACCTA
ACTGTACGTGAAACTGTCAATGTTATTACTCTATACAAAGATCTCAAACCTGTTTTGGAT
TCATATGTTTTTAACGATGGCAGTTCCAGGGAACTAATGAACCTCACTGGAACAATCCCT
GTGCCTTATAGAGGTAATACATACAATATTCCAATATGCCTATGGCTACTGGACACATAC
CCATATAATCCCCCTATCTGTTTTGTTAAGCCTACTAGTTCAATGACTATTAAAACAGGA
AAGCATGTTGATGCAAATGGGAAGATATATCTTCCTTATCTACATGAATGGAAACACCCA
CAGTCAGACTTGTTGGGGCTTATTCAGGTCATGATTGTGGTATTTGGAGATGAACCTCCA
GTCTTCTCTCGTCCTATTTCGGCATCCTATCCGCCATACCAGGCAACGGGGCCACCAAAT
ACTTCCTACATGCCAGGCATGCCAGGTGGAATCTCTCCATACCCATCCGGATACCCTCCC
AATCCCAGTGGTTACCCAGGCTGTCCTTACCCACCTGGTGGTCCATATCCTGCCACAACA
AGTTCTCAGTACCCTTCTCAGCCTCCTGTGACCACTGTTGGTCCCAGTAGGGATGGCACA
ATCAGCGAGGACACCATCCGAGCCTCTCTCATCTCTGCGGTCAGTGACAAACTGAGATGG
CGGATGAAGGAGGAAATGGATCGTGCCCAGGCAGAGCTCAATGCCTTGAAACGAACAGAA
GAAGACCTGAAAAAGGGTCACCAGAAACTGGAAGAGATGGTTACCCGTTTAGATCAAGAA
GTAGCCGAGGTTGATAAAAACATAGAACTTTTGAAAAAGAAGGATGAAGAACTCAGTTCT
GCTCTGGAAAAAATGGAAAATCAGTCTGAAAACAATGATATCGATGAAGTTATCATTCCC
ACAGCTCCCTTATACAAACAGATCCTGAATCTGTATGCAGAAGAAAACGCTATTGAAGAC
ACTATCCTTTACTTGGGAGAAGCCTTGAGAAGGGGCGTGATAGACCTGGATGTCTTCCTG
AAGCATGTACGTCTTCTGTCCCGTAAACAGTTCCAGCTGAGGGCACTAATGCAAAAAGCA
AGAAAGACTGCCGGTCTCAGTGACCTCTACTTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1257
Start on reference sequence 85
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
(goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR201

pDONR201 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  pDONR201-forward
Reverse:  pDONR201-reverse
Description: Recombinational donor/master vector; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4470
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 (pUC-type) origin of replication (modified, low copy number) 3744 4426
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1264 959
primer site forward primer recommended forward primer site 5 tcgcgttaacgctagcatggatctc 3 300 324
primer site reverse primer recommended reverse primer site 5 gtaacatcagagattttgagacac 3 2769 2792
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2744 2513
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 332 563
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2744 2513
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 332 563
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2265 2513
selectable marker KanR kanamycin resistance gene 2868 3677
trxn termination sequence rrn T2 rrn T2 transcription termination sequence 73 100
trxn termination sequence rrn T1 rrn T1 transcription termination sequence 232 275

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : TSG101
Symbol Nomenclature : TSG101
SYNONYM : TSG10; VPS23
Designation : ESCRT-I complex subunit TSG101|tumor susceptibility gene 10|tumor susceptibility gene 101|tumor susceptibility gene 101 protein|tumor susceptibility protein
Full Nomenclature : tumor susceptibility 101
GENEID : 7251
GI : 60825773
GenBank Accession : AY893696
HGNC : 15971
MIM : 601387
Vega : OTTHUMG00000167725
Target GenBank: BC002487

 

Reference Sequence Alignment

  

HsCD00000245      ATGGCGGTGTCGGAGAGCCAGCTCAAGAAAATGGTGTCCAAGTACAAATACAGAGACCTA
NM_006292.3       ATGGCGGTGTCGGAGAGCCAGCTCAAGAAAATGGTGTCCAAGTACAAATACAGAGACCTA
                  ************************************************************

HsCD00000245      ACTGTACGTGAAACTGTCAATGTTATTACTCTATACAAAGATCTCAAACCTGTTTTGGAT
NM_006292.3       ACTGTACGTGAAACTGTCAATGTTATTACTCTATACAAAGATCTCAAACCTGTTTTGGAT
                  ************************************************************

HsCD00000245      TCATATGTTTTTAACGATGGCAGTTCCAGGGAACTAATGAACCTCACTGGAACAATCCCT
NM_006292.3       TCATATGTTTTTAACGATGGCAGTTCCAGGGAACTAATGAACCTCACTGGAACAATCCCT
                  ************************************************************

HsCD00000245      GTGCCTTATAGAGGTAATACATACAATATTCCAATATGCCTATGGCTACTGGACACATAC
NM_006292.3       GTGCCTTATAGAGGTAATACATACAATATTCCAATATGCCTATGGCTACTGGACACATAC
                  ************************************************************

HsCD00000245      CCATATAATCCCCCTATCTGTTTTGTTAAGCCTACTAGTTCAATGACTATTAAAACAGGA
NM_006292.3       CCATATAATCCCCCTATCTGTTTTGTTAAGCCTACTAGTTCAATGACTATTAAAACAGGA
                  ************************************************************

HsCD00000245      AAGCATGTTGATGCAAATGGGAAGATATATCTTCCTTATCTACATGAATGGAAACACCCA
NM_006292.3       AAGCATGTTGATGCAAATGGGAAGATATATCTTCCTTATCTACATGAATGGAAACACCCA
                  ************************************************************

HsCD00000245      CAGTCAGACTTGTTGGGGCTTATTCAGGTCATGATTGTGGTATTTGGAGATGAACCTCCA
NM_006292.3       CAGTCAGACTTGTTGGGGCTTATTCAGGTCATGATTGTGGTATTTGGAGATGAACCTCCA
                  ************************************************************

HsCD00000245      GTCTTCTCTCGTCCTATTTCGGCATCCTATCCGCCATACCAGGCAACGGGGCCACCAAAT
NM_006292.3       GTCTTCTCTCGTCCTATTTCGGCATCCTATCCGCCATACCAGGCAACGGGGCCACCAAAT
                  ************************************************************

HsCD00000245      ACTTCCTACATGCCAGGCATGCCAGGTGGAATCTCTCCATACCCATCCGGATACCCTCCC
NM_006292.3       ACTTCCTACATGCCAGGCATGCCAGGTGGAATCTCTCCATACCCATCCGGATACCCTCCC
                  ************************************************************

HsCD00000245      AATCCCAGTGGTTACCCAGGCTGTCCTTACCCACCTGGTGGTCCATATCCTGCCACAACA
NM_006292.3       AATCCCAGTGGTTACCCAGGCTGTCCTTACCCACCTGGTGGTCCATATCCTGCCACAACA
                  ************************************************************

HsCD00000245      AGTTCTCAGTACCCTTCTCAGCCTCCTGTGACCACTGTTGGTCCCAGTAGGGATGGCACA
NM_006292.3       AGTTCTCAGTACCCTTCTCAGCCTCCTGTGACCACTGTTGGTCCCAGTAGGGATGGCACA
                  ************************************************************

HsCD00000245      ATCAGCGAGGACACCATCCGAGCCTCTCTCATCTCTGCGGTCAGTGACAAACTGAGATGG
NM_006292.3       ATCAGCGAGGACACCATCCGAGCCTCTCTCATCTCTGCGGTCAGTGACAAACTGAGATGG
                  ************************************************************

HsCD00000245      CGGATGAAGGAGGAAATGGATCGTGCCCAGGCAGAGCTCAATGCCTTGAAACGAACAGAA
NM_006292.3       CGGATGAAGGAGGAAATGGATCGTGCCCAGGCAGAGCTCAATGCCTTGAAACGAACAGAA
                  ************************************************************

HsCD00000245      GAAGACCTGAAAAAGGGTCACCAGAAACTGGAAGAGATGGTTACCCGTTTAGATCAAGAA
NM_006292.3       GAAGACCTGAAAAAGGGTCACCAGAAACTGGAAGAGATGGTTACCCGTTTAGATCAAGAA
                  ************************************************************

HsCD00000245      GTAGCCGAGGTTGATAAAAACATAGAACTTTTGAAAAAGAAGGATGAAGAACTCAGTTCT
NM_006292.3       GTAGCCGAGGTTGATAAAAACATAGAACTTTTGAAAAAGAAGGATGAAGAACTCAGTTCT
                  ************************************************************

HsCD00000245      GCTCTGGAAAAAATGGAAAATCAGTCTGAAAACAATGATATCGATGAAGTTATCATTCCC
NM_006292.3       GCTCTGGAAAAAATGGAAAATCAGTCTGAAAACAATGATATCGATGAAGTTATCATTCCC
                  ************************************************************

HsCD00000245      ACAGCTCCCTTATACAAACAGATCCTGAATCTGTATGCAGAAGAAAACGCTATTGAAGAC
NM_006292.3       ACAGCTCCCTTATACAAACAGATCCTGAATCTGTATGCAGAAGAAAACGCTATTGAAGAC
                  ************************************************************

HsCD00000245      ACTATCCTTTACTTGGGAGAAGCCTTGAGAAGGGGCGTGATAGACCTGGATGTCTTCCTG
NM_006292.3       ACTATCTTTTACTTGGGAGAAGCCTTGAGAAGGGGCGTGATAGACCTGGATGTCTTCCTG
                  ****** *****************************************************

HsCD00000245      AAGCATGTACGTCTTCTGTCCCGTAAACAGTTCCAGCTGAGGGCACTAATGCAAAAAGCA
NM_006292.3       AAGCATGTACGTCTTCTGTCCCGTAAACAGTTCCAGCTGAGGGCACTAATGCAAAAAGCA
                  ************************************************************

HsCD00000245      AGAAAGACTGCCGGTCTCAGTGACCTCTACTTG
NM_006292.3       AGAAAGACTGCCGGTCTCAGTGACCTCTACTGA
                  ******************************* .

Pathways

NCI : Glucocorticoid receptor regulatory network
NCI : Internalization of ErbB1
Reactome : Assembly Of The HIV Virion
Reactome : Budding and maturation of HIV virion
Reactome : Disease
Reactome : Endosomal Sorting Complex Required For Transport (ESCRT)
Reactome : HIV Infection
Reactome : HIV Life Cycle
Reactome : Late Phase of HIV Life Cycle
Reactome : Membrane Trafficking
Reactome : Membrane binding and targetting of GAG proteins

Clone

Cloning Information : 56692
HIP Master Clone ID : 34980
Original Clone ID : FLH056692.01L

Organism


TAX_ID : 9606
Species Specific ID: 7251

Genome

Chromosome : 11
Map Location : 11p15
Ensembl : ENSG00000074319

Reagents

Labome : TSG101-antibody