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Detailed Vector Information: pFA6a-mRFP1-kanMX6

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Explanation of Terms


Gene: Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID: pFA6a-mRFP1-kanMX6
Type: No insert
Vector Name: pFA6a-mRFP1-kanMX6              
Source: O'Shea lab (HU/HMS)
Description: Yeast fluorescent (RFP) vector with PTEF promoter; amp resistance (kanamycin for yeast); restriction enzyme cloning.
Comments: Similar but not identical to pKT146, pKT176, pKT178 clones also in the repository collection.
Publications: PMID: 14562095
Title: Global analysis of protein localization in budding yeast
Authors: Erin O'Shea
James Falvo
WK Huh
EvNO00025949

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CPD only
Special MTA: TSIEN

 

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  yeast    Marker:  kanamycin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
       DyNA Vector Map

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Vector Name: pFA6a-mRFP1-kanMX6

pFA6a-mRFP1-kanMX6 in advanced viewed

Synonyms: pFA6a-RFP-kanR
Description: Yeast fluorescent (RFP) vector with PTEF promoter; amp resistance (kanamycin for yeast); restriction enzyme cloning.
Comments: None
Size (bp): 4846
Parent Vector: None
Properties: fluorescent marker, multiple cloning site, yeast expression, yeast shuttle
Author Name: Erin O'Shea
WK Huh
Publications: PMID: 14562095
Title: Global analysis of protein localization in budding yeast

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColEI ori ColE1-type bacterial origin of replication 2742 3424
primer site T7 primer site T7 primer binding site 2483 2501
promoter PTEF PTEF eukaryotic promoter (inferred from vector family) 0 0
reporter gene RFP red fluorescent protein (FRP) 63 737
selectable marker AmpR ampicillin resistance gene (bla) 3522 4181
selectable marker KanR kanamycin resistance gene (for yeast) 1367 2173

 

Original Clone ID: pFA6a-mRFP1-kanMX6
SYNONYM: pFA6a-RFP-kanR
Species Specific ID: None
Target GenBank: None