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LGALS4 (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  LGALS4
Gene Name:  lectin, galactoside-binding, soluble, 4
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH110772.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / Yes
Publications: None
Authors: HIP
HsCD00002732

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 972nts         Open reading frame : 1 to 972
Reference Sequence Alignment

ATGGCCTATGTCCCAGCACCGGGCTACCAGCCCACCTACAACCCGACGCTGCCTTACTAC
CAGCCCATCCCGGGCGGGCTCAACGTGGGAATGTCTGTTTACATCCAAGGAGTGGCCAGC
GAGCACATGAAGCGGTTCTTCGTGAACTTTGTGGTTGGGCAGGATCCGGGCTCAGACGTC
GCCTTCCACTTCAATCCGCGGTTTGACGGCTGGGACAAGGTGGTCTTCAACACGTTGCAG
GGCGGGAAGTGGGGCAGCGAGGAGAGGAAGAGGAGCATGCCCTTCAAAAAGGGTGCCGCC
TTTGAGCTGGTCTTCATAGTCCTGGCTGAGCACTACAAGGTGGTGGTAAATGGAAATCCC
TTCTATGAGTACGGGCACCGGCTTCCCCTACAGATGGTCACCCACCTGCAAGTGGATGGG
GATCTGCAACTTCAATCAATCAACTTCATCGGAGGCCAGCCCCTCCGGCCCCAGGGACCC
CCGATGATGCCACCTTACCCTGGTCCCGGACATTGCCATCAACAGCTGAACAGCCTGCCC
ACCATGGAAGGACCCCCAACCTTCAACCCGCCTGTGCCATATTTCGGGAGGCTGCAAGGA
GGGCTCACAGCTCGAAGAACCATCATCATCAAGGGCTATGTGCCTCCCACAGGCAAGAGC
TTTGCTATCAACTTCAAGGTGGGCTCCTCAGGGGACATAGCTCTGCACATTAATCCCCGC
ATGGGCAACGGTACCGTGGTCCGGAACAGCCTTCTGAATGGCTCGTGGGGATCCGAGGAG
AAGAAGATCACCCACAACCCATTTGGTCCCGGACAGTTCTTTGATCTGTCCATTCGCTGT
GGCTTGGATCGCTTCAAGGTTTACGCCAATGGCCAGCACCTCTTTGACTTTGCCCATCGC
CTCTCGGCCTTCCAGAGGGTGGACACATTGGAAATCCAGGGTGATGTCACCTTGTCCTAT
GTCCAGATCTTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1024
Start on reference sequence 53
Mutations:
None
Discrepancy:
c15a
                                

5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 1015 1048
recombination site loxP site loxP recombination site 9 42
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : LGALS4
Symbol Nomenclature : LGALS4
SYNONYM : GAL4; L36LBP
Designation : L-36 lactose-binding protein|antigen NY-CO-27|gal-4|galectin 4|galectin-4|lactose-binding lectin 4
Full Nomenclature : lectin, galactoside-binding, soluble, 4
GENEID : 3960
GI : 61371620
GenBank Accession : AY891774
HGNC : 6565
MIM : 602518
Vega : OTTHUMG00000182608
Target GenBank: BC005146

 

Reference Sequence Alignment

  

HsCD00002732      ATGGCCTATGTCCCAGCACCGGGCTACCAGCCCACCTACAACCCGACGCTGCCTTACTAC
NM_006149.3       ATGGCCTATGTCCCCGCACCGGGCTACCAGCCCACCTACAACCCGACGCTGCCTTACTAC
                  **************.*********************************************

HsCD00002732      CAGCCCATCCCGGGCGGGCTCAACGTGGGAATGTCTGTTTACATCCAAGGAGTGGCCAGC
NM_006149.3       CAGCCCATCCCGGGCGGGCTCAACGTGGGAATGTCTGTTTACATCCAAGGAGTGGCCAGC
                  ************************************************************

HsCD00002732      GAGCACATGAAGCGGTTCTTCGTGAACTTTGTGGTTGGGCAGGATCCGGGCTCAGACGTC
NM_006149.3       GAGCACATGAAGCGGTTCTTCGTGAACTTTGTGGTTGGGCAGGATCCGGGCTCAGACGTC
                  ************************************************************

HsCD00002732      GCCTTCCACTTCAATCCGCGGTTTGACGGCTGGGACAAGGTGGTCTTCAACACGTTGCAG
NM_006149.3       GCCTTCCACTTCAATCCGCGGTTTGACGGCTGGGACAAGGTGGTCTTCAACACGTTGCAG
                  ************************************************************

HsCD00002732      GGCGGGAAGTGGGGCAGCGAGGAGAGGAAGAGGAGCATGCCCTTCAAAAAGGGTGCCGCC
NM_006149.3       GGCGGGAAGTGGGGCAGCGAGGAGAGGAAGAGGAGCATGCCCTTCAAAAAGGGTGCCGCC
                  ************************************************************

HsCD00002732      TTTGAGCTGGTCTTCATAGTCCTGGCTGAGCACTACAAGGTGGTGGTAAATGGAAATCCC
NM_006149.3       TTTGAGCTGGTCTTCATAGTCCTGGCTGAGCACTACAAGGTGGTGGTAAATGGAAATCCC
                  ************************************************************

HsCD00002732      TTCTATGAGTACGGGCACCGGCTTCCCCTACAGATGGTCACCCACCTGCAAGTGGATGGG
NM_006149.3       TTCTATGAGTACGGGCACCGGCTTCCCCTACAGATGGTCACCCACCTGCAAGTGGATGGG
                  ************************************************************

HsCD00002732      GATCTGCAACTTCAATCAATCAACTTCATCGGAGGCCAGCCCCTCCGGCCCCAGGGACCC
NM_006149.3       GATCTGCAACTTCAATCAATCAACTTCATCGGAGGCCAGCCCCTCCGGCCCCAGGGACCC
                  ************************************************************

HsCD00002732      CCGATGATGCCACCTTACCCTGGTCCCGGACATTGCCATCAACAGCTGAACAGCCTGCCC
NM_006149.3       CCGATGATGCCACCTTACCCTGGTCCCGGACATTGCCATCAACAGCTGAACAGCCTGCCC
                  ************************************************************

HsCD00002732      ACCATGGAAGGACCCCCAACCTTCAACCCGCCTGTGCCATATTTCGGGAGGCTGCAAGGA
NM_006149.3       ACCATGGAAGGACCCCCAACCTTCAACCCGCCTGTGCCATATTTCGGGAGGCTGCAAGGA
                  ************************************************************

HsCD00002732      GGGCTCACAGCTCGAAGAACCATCATCATCAAGGGCTATGTGCCTCCCACAGGCAAGAGC
NM_006149.3       GGGCTCACAGCTCGAAGAACCATCATCATCAAGGGCTATGTGCCTCCCACAGGCAAGAGC
                  ************************************************************

HsCD00002732      TTTGCTATCAACTTCAAGGTGGGCTCCTCAGGGGACATAGCTCTGCACATTAATCCCCGC
NM_006149.3       TTTGCTATCAACTTCAAGGTGGGCTCCTCAGGGGACATAGCTCTGCACATTAATCCCCGC
                  ************************************************************

HsCD00002732      ATGGGCAACGGTACCGTGGTCCGGAACAGCCTTCTGAATGGCTCGTGGGGATCCGAGGAG
NM_006149.3       ATGGGCAACGGTACCGTGGTCCGGAACAGCCTTCTGAATGGCTCGTGGGGATCCGAGGAG
                  ************************************************************

HsCD00002732      AAGAAGATCACCCACAACCCATTTGGTCCCGGACAGTTCTTTGATCTGTCCATTCGCTGT
NM_006149.3       AAGAAGATCACCCACAACCCATTTGGTCCCGGACAGTTCTTTGATCTGTCCATTCGCTGT
                  ************************************************************

HsCD00002732      GGCTTGGATCGCTTCAAGGTTTACGCCAATGGCCAGCACCTCTTTGACTTTGCCCATCGC
NM_006149.3       GGCTTGGATCGCTTCAAGGTTTACGCCAATGGCCAGCACCTCTTTGACTTTGCCCATCGC
                  ************************************************************

HsCD00002732      CTCTCGGCCTTCCAGAGGGTGGACACATTGGAAATCCAGGGTGATGTCACCTTGTCCTAT
NM_006149.3       CTCTCGGCCTTCCAGAGGGTGGACACATTGGAAATCCAGGGTGATGTCACCTTGTCCTAT
                  ************************************************************

HsCD00002732      GTCCAGATCTTG
NM_006149.3       GTCCAGATCTAA
                  **********:.

Protein

SMART domain : GLECT : Galectin
SMART domain : Gal-bind_lectin : Galactoside-binding lectin
UniProt : Q6FHZ4
UniProt : P56470
HPRD : 03949

Clone

Cloning Information : 110772
HIP Master Clone ID : 87055
Original Clone ID : FLH110772.01L

Organism


TAX_ID : 9606
Species Specific ID: 3960

Genome

Chromosome : 19
Map Location : 19q13.2
Ensembl : ENSG00000171747

Reagents

Labome : galectin-4-antibody