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CFTR (Homo sapiens) in pSGX-TOPO (His-tagged baculovirus expression vector)

TOP

Explanation of Terms


Gene: Gene Symbol:  CFTR
Gene Name:  cystic fibrosis transmembrane conductance regulator (ATP-binding cassette sub-family C, member 7)
Sequence             
Original Clone ID: 2935c553KFg2h2
Keyword: Partial Protein, NBD1 domain
Species: Homo sapiens
Type: cDNA
Vector Name: pSGX-TOPO               Format:  FUSION
Source: SGX Pharmaceuticals, Inc.
Description: This clone encodes the NBD1 domain with other intended mutations and corresponds to region 388-646 on full length protein NP_000483.3.
Comments: N-terminal 6-His, cleavable, TOPO. Insert sequence of this clone was provided by clone submitter and was not fully sequence verified.
Mutation/
Discrepancy :
Yes / Unknown
Publications: None
Authors: SGX Pharmaceuticals, Inc.
HsCD00287206

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AVAILABLE
No restriction
Special MTA: PSI

 

Insert sequence: 858nts         Open reading frame : 1 to 858

ATGTCGTACTACCATCACCATCACCATCACGATTACGATCTACTAACGACCGAAAACCTG
TATTTTCAGGGATCCCTTACAAGCTTAAAAGGACTATGGACACTTCGTGCCTTCGGACGG
CAGCCTTCCGGTGGTGGTAGCGGAGGAGGATCTGGCGGCGGCTCAGGGGGCGGGACGACT
ACAGAAGTAGTGATGGAGAATGTAACAGCCTTCTGGGAGGAGGGAGGTACTCCTGTCCTG
AAAGATATTAATTTCAAGATAGAAAGAGGACAGTTGTTGGCGGTTGCTGGATCCACTGGA
GCAGGCAAGACTTCACTTCTAATGATGATTATGGGAGAACTGGAGCCTTCAGAGGGTAAA
ATTAAGCACAGTGGAAGAATTTCATTCTGTTCTCAGTTTTCCTGGATTATGCCTGGCACC
ATTAAAGAAAATATCATCTTTGGTGTTTCCTATGATGAATATAGATACAGAAGCGTCATC
AAAGCATGCCAACTAGAAGAAGACATCTCCAAGTTTGCAGAGAAAGACAATATAGTTCTT
GGAGAAGGTGGAATCACACTGAGTGGAGGTCAACGAGCAAGAATTTCTTTAGCAAGAGCA
GTATACAAAGATGCTGATTTGTATTTATTAGACTCTCCTTTTGGATACCTAGATGTTTTA
ACAGAAAAAGAAATATTTGAAAGCTGTGTCTGTAAACTGATGGCTAACAAAACTAGGATT
TTGGTCACTTCTAAAATGGAACATTTAAAGAAAGCTGACAAAATATTAATTTTGCATGAA
GGTAGCAGCTATTTTTATGGGACATTTTCAGAACTCCAAAATCTACAGCCAGACTTTAGC
TCAAAACTCATGGGATAA


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: End read sequencing of cDNA insert
Reference Sequence Annotations:
End on protein reference sequence 646
Start on protein reference sequence 388
Mutations:
387ins31, del405-436, N620H*

ins@388,31

Discrepancy:
None
Others:
Domain NBD1
Termini 388-646
5' Linker Sequence:
CGACCGAAAACCTGTATTTTCAGGGATCCCTT

3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  insect cells    Marker:  gentamicin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in 2935c
Recommended expression in: SF9
       DyNA Vector Map

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Vector Name: pSGX-TOPO

pSGX-TOPO in advanced viewed

Synonyms: pSGX-TOPO_KF
Sequencing Primer: Forward:  pFB forward
Reverse:  pFB reverse
Description: Baculovirus vector, adds N-terminal 6xHis tag and TEV protease site; ampicillin resistance in bacteria (gentamicin resistance in insect cells); TOPO cloning.
Comments: pFastBAC topogated N-His with TEV.
Size (bp): 4793
Parent Vector: None
Empty Vector: None
Properties: TOPO Cloning, baculovirus/insect cell expression, with tag/fusion/marker
Author Name: New York SGX Research Center for Structural Genomics
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
ATG start start initiation ATG 4050 4052
TOPO recognition site TOPO TOPO cloning site 4123 4132
bacterial origin ori pUC-type origin of replication 1544 2226
poly-A signal polyA SV40 poly-A signal sequence 4222 4413
primer pFB reverse reverse sequencing primer: ACAAATGTGGTATGGCTGATT 4183 4203
primer pFB foward forward sequencing primer: TATTCCGGATTATTCATACC 3995 4014
promoter PH polyhedrin promoter 3904 4032
promoter Pc Pc promoter 0 0
selectable marker AmpR ampicillin resistance gene 787 1446
selectable marker GenR gentamycin resistance gene 3000 3533
ssDNA origin f1 ori f1 origin 19 325
tag His 6xHis tag 4062 4079
transposon fragment Tn7R Tn7R transposon sequence 2709 2933
transposon fragment Tn7L Tn7L transposon sequence 0 0

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Experimental
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CFTR
Symbol Nomenclature : CFTR
SYNONYM : ABC35; ABCC7; CF; CFTR/MRP; MRP7; TNR-CFTR; dJ760C5.1
Designation : cAMP-dependent chloride channel|channel conductance-controlling ATPase|cystic fibrosis transmembrane conductance regulator
Full Nomenclature : cystic fibrosis transmembrane conductance regulator (ATP-binding cassette sub-family C, member 7)
GENEID : 1080
Locus Tag : tcag7.78
GenBank Accession : NP_000483.3
HGNC : 1884
MIM : 602421
Vega : OTTHUMG00000023076
Target GenBank: NP_000483.3

 

Pathways

Reactome : ABC-family proteins mediated transport
Reactome : Transmembrane transport of small molecules

Protein

Domain : NBD1
SMART domain : AAA : ATPases associated with a variety of cellular activities
Structural Annotation Wiki : TOPSAN
UniProt : P13569
HPRD : 03883

Clone

Cloning Information : 473956
HIP Master Clone ID : 395462
Original Clone ID : 2935c553KFg2h2

Experimental

TargetTrack - Experimental data : 2935c

Organism


TAX_ID : 9606
Species Specific ID: 1080

Genome

Chromosome : 7
Map Location : 7q31.2
Ensembl : ENSG00000001626

Reagents

Labome : CFTR-antibody