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CFTR (Ovis aries) in pSGX4 (His and Smt3-tagged bacterial expression vector)

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Explanation of Terms


Gene: Gene Symbol:  CFTR
Gene Name:  cystic fibrosis transmembrane conductance regulator (ATP-binding cassette sub-family C, member 7)
Sequence             
Original Clone ID: 2935g4BSt5b1
Keyword: Partial Protein, NBD1 domain
Species: Ovis aries
Type: cDNA
Vector Name: pSGX4               Format:  FUSION
Source: SGX Pharmaceuticals, Inc.
Description: This clone encodes the NBD1 domain and corresponds to region 389-669 on full length protein AAA68600.1.
Comments: N-terminal 6-His and Smt3, cleavable, TOPO. Insert sequence of this clone was provided by clone submitter and was not fully sequence verified.
Mutation/
Discrepancy :
No / Unknown
Publications: None
Authors: SGX Pharmaceuticals, Inc.
OaCD00287532

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AVAILABLE
No restriction
Special MTA: PSI/Smt3/SUMO

 

Insert sequence: 852nts        
Open reading frame : 1 to 852

ACCACAGATGTAGTCATGGAAAACGTGACGGCCTTCTGGGAAGAGGGCTTTTCGAAACTT
TTCGAAAAAGCAAAAGAAAATAATAATAACCGCAAAATCTCAAACTGCGACACGTCCCTG
TTTTTTTCCAACTTGCTGCTTGGCACGCCGGTGCTTAAAGATATCTCATTCAAAATCGAA
CGGGGCCAACTGCTGGCAGTAGCGGGCAGCACAGGAGCAGGTAAAACCTCTCTGCTGATG
ATGATCATGGGCGAACTGGAACCAAGCGAGGGGAAAATTAAACACTCGGGGCGCATCTCT
TTTTGCTCGCAATATAGCTGGATCATGCCGGGCACCATCAAAGACAACATTATTTTTGGC
GTGTCGTATGACGAATATCGCTATCGCTCCGTCATCAAAGCGTGCCAGCTTGAGGAAGAC
ATTTCTAAATTCTCTGAAAAGGACAATATCGTCCTGGGCGAAGGCGGCATCACCCTGTCA
GGTGGTCAGCGCGCACGCATTTCGTTAGCCCGTGCGGTGTATAAGGATGCGGATCTGTAC
CTCCTGGACTCACCGTTCGGTTACTTAGATGTTCTGACTGAGAAAGAGATTTTTGAATCT
TGCGTGTGTAAACTTATGGCGAATAAGACCCGAATTCTGGTCACCTCGAAAATGGAGCAC
CTTAAAAAGGCGGACAAGATTTTAATCCTGCACGAGGGCTCGGTATATTTCTATGGTACA
TTTTCAGAACTGCAGAATCAGCGCCCTGATTTTTCATCTAAATTGATGGGTTGCGATACG
TTCGACCAGTTTACCGCTGAACGTCGTAATTCGATTATTACTGAGACCCTGCGCCGTTTT
TCCGAAGGGTAA


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: End read sequencing of cDNA insert
Reference Sequence Annotations:
Start on protein reference sequence 389
End on protein reference sequence 669
Mutations:
None
Discrepancy:
None
Others:
Termini 389-669
Domain NBD1
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in 2935g
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pSGX4

pSGX4 in advanced viewed

Synonyms: pSGX-4, pSGX4_BS
Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Bacterial (E. coli) expression vector with T7 promotor, adds N-terminal 6xHis and smt-3 tags, cleavage site; kanamycin resistance; TOPO cloning.
Comments: Modified from pET26b from Novagen. N-terminal 6xHis-tag followed by smt3 fusion protein. Smt3 fusion cloned into NheI and BamHI sites. TOPO adaptation at BamHI and XhoI sites.
Size (bp): 5611
Parent Vector: None
Empty Vector: None
Properties: TOPO Cloning, bacterial expression, in vitro transcription, with tag/fusion/marker
Author Name: New York SGX Research Center for Structural Genomics
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
TOPO recognition site TOPO TOPO cloning site 5435 5444
bacterial origin ColE ColE1 bacterial origin of replication 1421 2103
gene fragment ROP ROP 2527 2703
promoter T7 T7 promoter 4984 5003
protease cleavage site ULP site ULP cleavage site (recognizes the tertiary structure of SUMO) 5140 5140
repressor protein gene LacI lacI cds 3515 4351
ribosome binding site RBS ribosome binding site (RBS) 5062 5068
selectable marker KanR kanamycin resistance gene 563 1375
tag Smt3 Smt3 tag 5140 5424
tag His 6xHis tag 5083 5100
trxn regulatory element lac operator lac operator 5003 5025
trxn termination sequence T7 term T7 termination sequence 5539 5585

 

  • Gene
  • Protein
  • Clone
  • Experimental
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CFTR
Designation : ATP-binding cassette sub-family C member 7|ATP-binding cassette transporter sub-family C member 7|cAMP-dependent chloride channel|channel conductance-controlling ATPase|cystic fibrosis transmembrane conductance regulator|cystic fibrosis transmembrane conductance regulator, ATP-binding cassette (sub-family C, member 7)
GENEID : 443347
GenBank Accession : AAA68600.1
Target GenBank: AAA68600.1

 

Protein

Domain : NBD1
SMART domain : AAA : ATPases associated with a variety of cellular activities
Structural Annotation Wiki : TOPSAN
UniProt : Q00555

Clone

Cloning Information : 474283
HIP Master Clone ID : 395789
Original Clone ID : 2935g4BSt5b1

Experimental

TargetTrack - Experimental data : 2935g

Organism


TAX_ID : 9940
Species Specific ID: 443347

Genome

Chromosome : 4
Ensembl : ENSOARG00000018133

Reagents

Labome : CFTR-cDNA