DNASU Plasmid Repository • 480.965.2857 | Email

CFTR (Homo sapiens) in pSGX5 (His and Smt3-tagged bacterial expression vector)

TOP

Explanation of Terms


Gene: Gene Symbol:  CFTR
Gene Name:  cystic fibrosis transmembrane conductance regulator (ATP-binding cassette sub-family C, member 7)
Sequence             
Original Clone ID: 2935c328CLt1p1
Keyword: Partial Protein, NBD1 domain
Species: Homo sapiens
Type: cDNA
Vector Name: pSGX5               Format:  FUSION
Source: SGX Pharmaceuticals, Inc.
Description: This clone encodes the NBD1 domain with other intended mutations and corresponds to region 389-678 on full length protein NP_000483.3.
Comments: N-terminal 6-His-tag/SMT. Insert sequence of this clone was provided by clone submitter and was not fully sequence verified.
Mutation/
Discrepancy :
Yes / Unknown
Publications: PMID: 15528182
Title: Impact of the deltaF508 mutation in first nucleotide-binding domain of human cystic fibrosis transmembrane conductance regulator on domain folding and structure
Authors: SGX Pharmaceuticals, Inc.
HsCD00287566

Price:  Login for Pricing
AVAILABLE
No restriction
Special MTA: PSI/Smt3/SUMO

 

Insert sequence: 1236nts         Open reading frame : 1 to 1236

ATGGGCAGCAGCCATCATCATCATCATCACAGCAGCGGCCTGGTGCCGCGCGGCAGCCAT
ATGGCTAGCATGTCGGACTCAGAAGTCAATCAAGAAGCTAAGCCAGAGGTCAAGCCAGAA
GTCAAGCCTGAGACTCACATCAATTTAAAGGTGTCCGATGGATCTTCAGAGATCTTCTTC
AAGATCAAAAAGACCACTCCTTTAAGAAGGCTGATGGAAGCGTTCGCTAAAAGACAGGGT
AAGGAAATGGACTCCTTAAGATTCTTGTACGACGGTATTAGAATTCAAGCTGATCAGACC
CCTGAAGATTTGGACATGGAGGATAACGATATTATTGAGGCTCACAGAGAACAGATTGGT
GGATCAACTACAGAAGTAGTGATGGAGAATGTAACAGCCTTCTGGGAGGAGGGATTTGGG
GAATTATTAGAGAAAGCAAAACAAAACAATAACAATAGAAAAACTTCTAATGGTGATGAC
AGCCTCTCCTTCAGTAATTTATCACTTCTTGGTACTCCTGTCCTGAAAGATATTAATTTC
AAGATAGAAAGAGGACAGTTGTTGGCGGTTGCTGGATCCACTGGAGCAGGCAAGACTTCA
CTTCTAATGATGATTATGGGAGAACTGGAGCCTTCAGAGGGTAAAATTAAGCACAGTGGA
AGAATTTCATTCTGTTCTCAGTTTTCCTGGATTATGCCTGGCACCATTAAAGAAAATATC
ATCGGTGTTTCCTATGATGAATATAGATACAGAAGCGTCATCAAAGCATGCCAACTAGAA
GAAGACATCTCCAAGTTTGCAGAGAAAGACAATATAGTTCTTGGAGAAGGTGGAATCACA
CTGAGTGAAGGTCAACAAGCAAAGATTTCTTTAGCAAGAGCAGTATACAAAGATGCTGAT
TTGTATTTATTAGACTCTCCTTTTGGATACCTAGATGTTTTAACAGAAAAAGAAATATTT
GAAAGCTGTGTCTGTAAACTGATGGCTAACAAAACTAGGATTTTGGTCACTTCTAAAATG
GAACATTTAAAGAAAGCTGACAAAATATTAATTTTGCATGAAGGTAGCAGCTATTTTTAT
GGGACATTTTCAGAACTCCAAAATCTACAGCCAGACTTTAGCTCAAAACTCATGGGATGT
GATTCTTTCGACCAATTTAGTGCAGAAAGAAGAAATTCAATCCTAACTGAGACCTTACGC
CGTTTCTCATTAGAAGGAGATGCTCCTGTCTCCTAA


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: End read sequencing of cDNA insert
Reference Sequence Annotations:
End on protein reference sequence 678
Start on protein reference sequence 389
Mutations:


F409L; F429S; F433L; G550E; R553Q; R555K; H667R

Discrepancy:
None
Others:
Domain NBD1
Termini 389-678
5' Linker Sequence:
CACAGAGAACAGATTGGTGGATCC

3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Not_Tested
Find experimental protocols in 2935c
Recommended expression in: BL21(DE3)-Codon+RIL
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pSGX5

pSGX5 in advanced viewed

Synonyms: pSGX5
Description: Bacterial (E. coli) expression vector with T7 promotor, adds N-terminal 6xHis and smt-3 tags, ULP-1 cleavage site; kanamycin resistance; TOPO cloning.
Comments: Modified from pET26b from Novagen. Smt3 fusion at N-terminus. Clone using BamHI and either HindIII, XhoI or NotI
Size (bp): 5633
Parent Vector: None
Empty Vector: None
Properties: TOPO Cloning, bacterial expression, with tag/fusion/marker
Author Name: NYSGXRC
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
ATG start start start codon 5071 5073
bacterial origin ori pBR322-type bacterial origin of replication 1421 2103
gene fragment ROP ROP 2527 2703
primer site T7 T7 sequencing primers 4985 5000
promoter T7 T7 promoter 4984 5003
protease cleavage site ULP site ULP cleavage site cds 5242 5242
repressor protein gene LacI LacI gene 3515 4531
selectable marker KanR kanamycin resistance gene 563 1375
tag Smt3 N-terminal Smt3 tag 5140 5242
tag His 6xHis tag 5083 5100
trxn regulatory element lac operator lac operator 5003 5025
trxn termination sequence T7 term T7 termination sequence 5562 5608

 


Cloning Information: 474317
Domain: NBD1
GenBank Accession: NP_000483.3
HIP Master Clone ID: 395823
Labome: CFTR-antibody
Original Clone ID: 2935c328CLt1p1
SMART domain: AAA : ATPases associated with a variety of cellular activities
SMART domain: UBQ : Ubiquitin homologues
Structural Annotation Wiki: TOPSAN
TargetTrack - Experimental data: 2935c
Termini: 389-678
UniProt: P13569
Species Specific ID: 1080
Target GenBank: NP_000483.3