DNASU Plasmid | SIGLEC6 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: SIGLEC6

Gene Name: sialic acid binding Ig-like lectin 6

Original Clone ID:

13433

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

None

Comments:

None

Mutation / Discrepancy:

No / Yes

Publications:

None

Authors:

The ORFeome Collaboration

HsCD00287794

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1314nts

Open reading frame: 1 to 1314

Reference Sequence Alignment

ATGCAGGGAGCCCAGGAAGCCTCCGCCTCAGAGATGCTACCGCTGCTGCTGCCCCTGCTG
TGGGCAGGGGCCCTGGCTCAGGAGCGGAGATTCCAGCTGGAGGGGCCAGAGTCACTGACG
GTGCAGGAGGGTCTGTGCGTCCTCGTACCCTGCAGATTGCCCACTACCCTTCCAGCCTCG
TACTATGGTTATGGCTACTGGTTCCTGGAAGGGGCTGATGTTCCAGTGGCCACAAACGAC
CCAGACGAAGAAGTGCAGGAGGAGACCCGGGGCCGATTCCACCTCCTCTGGGATCCCAGA
AGGAAGAACTGCTCCCTGAGCATCAGAGATGCCCGGAGGAGGGACAATGCTGCATACTTC
TTTCGGTTGAAGTCCAAATGGATGAAATACGGTTATACATCTTCCAAGCTCTCTGTGCGT
GTGATGGCCCTGACCCACAGGCCCAACATCTCCATCCCAGGGACCCTGGAGTCTGGCCAT
CCCAGCAATCTGACCTGCTCTGTGCCCTGGGTCTGTGAGCAGGGGACGCCCCCCATCTTC
TCCTGGATGTCAGCTGCCCCCACCTCCCTGGGCCCCAGGACCACCCAGTCCTCGGTGCTC
ACAATCACCCCACGGCCCCAGGACCACAGCACCAACCTCACCTGTCAGGTGACGTTCCCT
GGAGCCGGTGTGACCATGGAGAGAACCATCCAGCTCAATGTCTCCTCCTTCAAAATCCTG
CAAAACACCTCGTCCCTCCCTGTCCTGGAGGGCCAGGCTCTGCGGCTGCTCTGTGATGCT
GACGGCAACCCCCCTGCACACCTGAGCTGGTTCCAGGGCTTCCCCGCCCTGAACGCCACC
CCCATCTCCAATACCGGGGTCCTGGAGCTGCCTCAAGTAGGGTCTGCAGAAGAAGGAGAT
TTCACCTGCCGTGCTCAGCATCCTCTGGGCTCCCTGCAAATCTCTCTGAGTCTCTTTGTG
CATTGGAAACCAGAAGGCAGGGCTGGTGGTGTCCTGGGAGCAGTCTGGGGAGCTAGCATC
ACAACCCTGGTTTTCCTCTGTGTTTGCTTCATCTTCAGAGTGAAGACTAGAAGGAAGAAA
GCAGCCCAGCCAGTGCAAAACACGGATGATGTGAACCCCGTCATGGTCTCAGGCTCCAGG
GGTCATCAGCACCAGTTCCAGACAGGCATAGTTTCAGACCACCCTGCTGAGGCTGGCCCC
ATCTCAGAAGATGAGCAGGAGCTCCACTACGCTGTCCTACACTTCCACAAGGTGCAACCT
CAGGAACCAAAGGTCACCGACACTGAGTACGCAGAAATCAAGATACACAAGTGC

Protein sequence predicted based on nucleotide sequence

MQGAQEASASEMLPLLLPLLWAGALAQERRFQLEGPESLTVQEGLCVLVPCRLPTTLPAS
YYGYGYWFLEGADVPVATNDPDEEVQEETRGRFHLLWDPRRKNCSLSIRDARRRDNAAYF
FRLKSKWMKYGYTSSKLSVRVMALTHRPNISIPGTLESGHPSNLTCSVPWVCEQGTPPIF
SWMSAAPTSLGPRTTQSSVLTITPRPQDHSTNLTCQVTFPGAGVTMERTIQLNVSSFKIL
QNTSSLPVLEGQALRLLCDADGNPPAHLSWFQGFPALNATPISNTGVLELPQVGSAEEGD
FTCRAQHPLGSLQISLSLFVHWKPEGRAGGVLGAVWGASITTLVFLCVCFIFRVKTRRKK
AAQPVQNTDDVNPVMVSGSRGHQHQFQTGIVSDHPAEAGPISEDEQELHYAVLHFHKVQP
QEPKVTDTEYAEIKIHKC

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Reference Sequence Annotations:

End on reference sequence 1383

Start on reference sequence 70

Mutations:

None

Discrepancy:

ins@1,33; t1291g,S431A

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

SIGLEC6

Symbol Nomenclature :

SIGLEC6

SYNONYM :

CD327; CD33L; CD33L1; CD33L2; CDW327; OBBP1

Designation :

CD33 antigen-like 1|obesity-binding protein 1|sialic acid-binding Ig-like lectin 6

Full Nomenclature :

sialic acid binding Ig-like lectin 6

GENEID :

946

HGNC :

10875

MIM :

604405

Vega :

OTTHUMG00000133571

Target GenBank:

BC035359.1

Reference Sequence Alignment

  

HsCD00287794      ATGCAGGGAGCCCAGGAAGCCTCCGCCTCAGAGATGCTACCGCTGCTGCTGCCCCTGCTG
NM_198845.4       ATGCAGGGAGCCCAGGAAGCCTCCGCCTCAGAGATGCTACCGCTGCTGCTGCCCCTGCTG
NM_001245.5       ATGCAGGGAGCCCAGGAAGCCTCCGCCTCAGAGATGCTACCGCTGCTGCTGCCCCTGCTG
                  ************************************************************

HsCD00287794      TGGGCAGGGGCCCTGGCTCAGGAGCGGAGATTCCAGCTGGAGGGGCCAGAGTCACTGACG
NM_198845.4       TGGGCAGGGGCCCTGGCTCAGGAGCGGAGATTCCAGCTGGAGGGGCCAGAGTCACTGACG
NM_001245.5       TGGGCAGGGGCCCTGGCTCAGGAGCGGAGATTCCAGCTGGAGGGGCCAGAGTCACTGACG
                  ************************************************************

HsCD00287794      GTGCAGGAGGGTCTGTGCGTCCTCGTACCCTGCAGATTGCCCACTACCCTTCCAGCCTCG
NM_198845.4       GTGCAGGAGGGTCTGTGCGTCCTCGTACCCTGCAGATTGCCCACTACCCTTCCAGCCTCG
NM_001245.5       GTGCAGGAGGGTCTGTGCGTCCTCGTACCCTGCAGATTGCCCACTACCCTTCCAGCCTCG
                  ************************************************************

HsCD00287794      TACTATGGTTATGGCTACTGGTTCCTGGAAGGGGCTGATGTTCCAGTGGCCACAAACGAC
NM_198845.4       TACTATGGTTATGGCTACTGGTTCCTGGAAGGGGCTGATGTTCCAGTGGCCACAAACGAC
NM_001245.5       TACTATGGTTATGGCTACTGGTTCCTGGAAGGGGCTGATGTTCCAGTGGCCACAAACGAC
                  ************************************************************

HsCD00287794      CCAGACGAAGAAGTGCAGGAGGAGACCCGGGGCCGATTCCACCTCCTCTGGGATCCCAGA
NM_198845.4       CCAGACGAAGAAGTGCAGGAGGAGACCCGGGGCCGATTCCACCTCCTCTGGGATCCCAGA
NM_001245.5       CCAGACGAAGAAGTGCAGGAGGAGACCCGGGGCCGATTCCACCTCCTCTGGGATCCCAGA
                  ************************************************************

HsCD00287794      AGGAAGAACTGCTCCCTGAGCATCAGAGATGCCCGGAGGAGGGACAATGCTGCATACTTC
NM_198845.4       AGGAAGAACTGCTCCCTGAGCATCAGAGATGCCCGGAGGAGGGACAATGCTGCATACTTC
NM_001245.5       AGGAAGAACTGCTCCCTGAGCATCAGAGATGCCCGGAGGAGGGACAATGCTGCATACTTC
                  ************************************************************

HsCD00287794      TTTCGGTTGAAGTCCAAATGGATGAAATACGGTTATACATCTTCCAAGCTCTCTGTGCGT
NM_198845.4       TTTCGGTTGAAGTCCAAATGGATGAAATACGGTTATACATCTTCCAAGCTCTCTGTGCGT
NM_001245.5       TTTCGGTTGAAGTCCAAATGGATGAAATACGGTTATACATCTTCCAAGCTCTCTGTGCGT
                  ************************************************************

HsCD00287794      GTGATGGCCCTGACCCACAGGCCCAACATCTCCATCCCAGGGACCCTGGAGTCTGGCCAT
NM_198845.4       GTGATGGCCCTGACCCACAGGCCCAACATCTCCATCCCAGGGACCCTGGAGTCTGGCCAT
NM_001245.5       GTGATGGCCCTGACCCACAGGCCCAACATCTCCATCCCAGGGACCCTGGAGTCTGGCCAT
                  ************************************************************

HsCD00287794      CCCAGCAATCTGACCTGCTCTGTGCCCTGGGTCTGTGAGCAGGGGACGCCCCCCATCTTC
NM_198845.4       CCCAGCAATCTGACCTGCTCTGTGCCCTGGGTCTGTGAGCAGGGGACGCCCCCCATCTTC
NM_001245.5       CCCAGCAATCTGACCTGCTCTGTGCCCTGGGTCTGTGAGCAGGGGACGCCCCCCATCTTC
                  ************************************************************

HsCD00287794      TCCTGGATGTCAGCTGCCCCCACCTCCCTGGGCCCCAGGACCACCCAGTCCTCGGTGCTC
NM_198845.4       TCCTGGATGTCAGCTGCCCCCACCTCCCTGGGCCCCAGGACCACCCAGTCCTCGGTGCTC
NM_001245.5       TCCTGGATGTCAGCTGCCCCCACCTCCCTGGGCCCCAGGACCACCCAGTCCTCGGTGCTC
                  ************************************************************

HsCD00287794      ACAATCACCCCACGGCCCCAGGACCACAGCACCAACCTCACCTGTCAGGTGACGTTCCCT
NM_198845.4       ACAATCACCCCACGGCCCCAGGACCACAGCACCAACCTCACCTGTCAGGTGACGTTCCCT
NM_001245.5       ACAATCACCCCACGGCCCCAGGACCACAGCACCAACCTCACCTGTCAGGTGACGTTCCCT
                  ************************************************************

HsCD00287794      GGAGCCGGTGTGACCATGGAGAGAACCATCCAGCTCAATG--------------------
NM_198845.4       GGAGCCGGTGTGACCATGGAGAGAACCATCCAGCTCAATG--------------------
NM_001245.5       GGAGCCGGTGTGACCATGGAGAGAACCATCCAGCTCAATGTCTCCTATGCTCCACAGAAA
                  ****************************************                    

HsCD00287794      ----------------------------TCTCCTCCTTCAAAATCCTGCAAAACACCTCG
NM_198845.4       ----------------------------TCTCCTCCTTCAAAATCCTGCAAAACACCTCG
NM_001245.5       GTGGCCATCAGCATCTTCCAAGGAAACAGCGCAGCCTTCAAAATCCTGCAAAACACCTCG
                                               * *. **************************

HsCD00287794      TCCCTCCCTGTCCTGGAGGGCCAGGCTCTGCGGCTGCTCTGTGATGCTGACGGCAACCCC
NM_198845.4       TCCCTCCCTGTCCTGGAGGGCCAGGCTCTGCGGCTGCTCTGTGATGCTGACGGCAACCCC
NM_001245.5       TCCCTCCCTGTCCTGGAGGGCCAGGCTCTGCGGCTGCTCTGTGATGCTGACGGCAACCCC
                  ************************************************************

HsCD00287794      CCTGCACACCTGAGCTGGTTCCAGGGCTTCCCCGCCCTGAACGCCACCCCCATCTCCAAT
NM_198845.4       CCTGCACACCTGAGCTGGTTCCAGGGCTTCCCCGCCCTGAACGCCACCCCCATCTCCAAT
NM_001245.5       CCTGCACACCTGAGCTGGTTCCAGGGCTTCCCCGCCCTGAACGCCACCCCCATCTCCAAT
                  ************************************************************

HsCD00287794      ACCGGGGTCCTGGAGCTGCCTCAAGTAGGGTCTGCAGAAGAAGGAGATTTCACCTGCCGT
NM_198845.4       ACCGGGGTCCTGGAGCTGCCTCAAGTAGGGTCTGCAGAAGAAGGAGATTTCACCTGCCGT
NM_001245.5       ACCGGGGTCCTGGAGCTGCCTCAAGTAGGGTCTGCAGAAGAAGGAGATTTCACCTGCCGT
                  ************************************************************

HsCD00287794      GCTCAGCATCCTCTGGGCTCCCTGCAAATCTCTCTGAGTCTCTTTGTGCATTGGAAACCA
NM_198845.4       GCTCAGCATCCTCTGGGCTCCCTGCAAATCTCTCTGAGTCTCTTTGTGCATTGGAAACCA
NM_001245.5       GCTCAGCATCCTCTGGGCTCCCTGCAAATCTCTCTGAGTCTCTTTGTGCATTGGAAACCA
                  ************************************************************

HsCD00287794      GAAGGCAGGGCTGGTGGTGTCCTGGGAGCAGTCTGGGGAGCTAGCATCACAACCCTGGTT
NM_198845.4       GAAGGCAGGGCTGGTGGTGTCCTGGGAGCAGTCTGGGGAGCTAGCATCACAACCCTGGTT
NM_001245.5       GAAGGCAGGGCTGGTGGTGTCCTGGGAGCAGTCTGGGGAGCTAGCATCACAACCCTGGTT
                  ************************************************************

HsCD00287794      TTCCTCTGTGTTTGCTTCATCTTCAGAGTGAAGACTAGAAGGAAGAAAGCAGCCCAGCCA
NM_198845.4       TTCCTCTGTGTTTGCTTCATCTTCAGAGTGAAGACTAGAAGGAAGAAAGCAGCCCAGCCA
NM_001245.5       TTCCTCTGTGTTTGCTTCATCTTCAGAGTGAAGACTAGAAGGAAGAAAGCAGCCCAGCCA
                  ************************************************************

HsCD00287794      GTGCAAAACACGGATGATGTGAACCCCGTCATGGTCTCAGGCTCCAGGGGTCATCAGCAC
NM_198845.4       GTGCAAAACACGGATGATGTGAACCCCGTCATGGTCTCAGGCTCCAGGGGTCATCAGCAC
NM_001245.5       GTGCAAAACACGGATGATGTGAACCCCGTCATGGTCTCAGGCTCCAGGGGTCATCAGCAC
                  ************************************************************

HsCD00287794      CAGTTCCAGACAGGCATAGTTTCAGACCACCCTGCTGAGGCTGGCCCCATCTCAGAAGAT
NM_198845.4       CAGTTCCAGACAGGCATAGTTTCAGACCACCCTGCTGAGGCTGGCCCCATCTCAGAAGAT
NM_001245.5       CAGTTCCAGACAGGCATAGTTTCAGACCACCCTGCTGAGGCTGGCCCCATCTCAGAAGAT
                  ************************************************************

HsCD00287794      GAGCAGGAGCTCCACTACGCTGTCCTACACTTCCACAAGGTGCAACCTCAGGAACCAAAG
NM_198845.4       GAGCAGGAGCTCCACTACGCTGTCCTACACTTCCACAAGGTGCAACCTCAGGAACCAAAG
NM_001245.5       GAGCAGGAGCTCCACTACGCTGTCCTACACTTCCACAAGGTGCAACCTCAGGAACCAAAG
                  ************************************************************

HsCD00287794      GTCACCGACACTGAGTACGCAGAAATCAAGATACACAAGTGC
NM_198845.4       GTCACCGACACTGAGTACTCAGAAATCAAGATACACAAGTGA
NM_001245.5       GTCACCGACACTGAGTACTCAGAAATCAAGATACACAAGTGA
                  ****************** **********************.