DNASU Plasmid | ASB8 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: ASB8

Gene Name: ankyrin repeat and SOCS box containing 8

Original Clone ID:

28367

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

The ORFeome Collaboration

HsCD00287938

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 867nts

Open reading frame: 1 to 867

Reference Sequence Alignment

ATGAGTTCCAGTATGTGGTATATTATGCAGAGCATTCAGAGCAAATACTCTCTCTCCGAG
CGCTTAATCCGAACAATTGCTGCCATCCGTTCCTTCCCACATGATAATGTAGAGGACCTC
ATCAGAGGGGGAGCAGATGTGAACTGCACTCATGGCACACTGAAGCCCTTGCACTGTGCC
TGTATGGTGTCAGATGCTGACTGTGTGGAGTTACTTCTGGAAAAAGGAGCCGAGGTGAAT
GCCCTGGATGGGTATAACCGAACAGCCCTCCACTATGCAGCAGAGAAAGATGAGGCTTGT
GTGGAGGTCCTATTGGAGTATGGTGCAAACCCCAATGCTTTGGATGGCAACAGAGATACC
CCACTTCACTGGGCAGCCTTTAAGAACAATGCTGAGTGTGTGCGGGCTCTCCTAGAGAGC
GGGGCCTCTGTCAATGCCCTGGATTACAACAATGATACACCGCTCAGCTGGGCTGCCATG
AAGGGAAATCTTGAGAGTGTCAGCATCCTTCTGGATTATGGCGCAGAGGTCAGAGTCATC
AACCTAATAGGCCAGACACCCATCTCCCGCCTGGTGGCTCTGCTAGTCAGGGGACTTGGA
ACAGAGAAAGAGGACTCTTGCTTTGAGCTCCTCCACAGAGCTGTTGGACACTTTGAATTG
AGGAAAAATGGCACCATGCCACGAGAGGTGGCCAGAGACCCGCAGCTATGTGAAAAACTG
ACTGTTCTGTGCTCAGCTCCAGGAACTCTAAAAACACTCGCTCGCTATGCCGTGCGCCGT
AGCCTGGGACTCCAGTATCTCCCCGATGCAGTGAAGGGCCTTCCACTGCCAGCTTCTTTG
AAGGAATACCTGTTACTTTTAGAATAC

Protein sequence predicted based on nucleotide sequence

MSSSMWYIMQSIQSKYSLSERLIRTIAAIRSFPHDNVEDLIRGGADVNCTHGTLKPLHCA
CMVSDADCVELLLEKGAEVNALDGYNRTALHYAAEKDEACVEVLLEYGANPNALDGNRDT
PLHWAAFKNNAECVRALLESGASVNALDYNNDTPLSWAAMKGNLESVSILLDYGAEVRVI
NLIGQTPISRLVALLVRGLGTEKEDSCFELLHRAVGHFELRKNGTMPREVARDPQLCEKL
TVLCSAPGTLKTLARYAVRRSLGLQYLPDAVKGLPLPASLKEYLLLLEY

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Reference Sequence Annotations:

Start on reference sequence 78

End on reference sequence 944

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

ASB8

Symbol Nomenclature :

ASB8

Designation :

ASB-8|ankyrin repeat and SOCS box protein 8|ankyrin repeat and SOCS box-containing 8

Full Nomenclature :

ankyrin repeat and SOCS box containing 8

GENEID :

140461

Locus Tag :

PP14212

HGNC :

17183

MIM :

615053

Vega :

OTTHUMG00000167834

Target GenBank:

BC001321.1

Reference Sequence Alignment

  

HsCD00287938        ATGAGTTCCAGTATGTGGTATATTATGCAGAGCATTCAGAGCAAATACTCTCTCTCCGAG
NM_024095.3         ATGAGTTCCAGTATGTGGTATATTATGCAGAGCATTCAGAGCAAATACTCTCTCTCCGAG
XM_005268654.1      ATGAGTTCCAGTATGTGGTATATTATGCAGAGCATTCAGAGCAAATACTCTCTCTCCGAG
                    ************************************************************

HsCD00287938        CGCTTAATCCGAACAATTGCTGCCATCCGTTCCTTCCCACATGATAATGTAGAGGACCTC
NM_024095.3         CGCTTAATCCGAACAATTGCTGCCATCCGTTCCTTCCCACATGATAATGTAGAGGACCTC
XM_005268654.1      CGCTTAATCCGAACAATTGCTGCCATCCGTTCCTTCCCACATGATAATGTAGAGGACCTC
                    ************************************************************

HsCD00287938        ATCAGAGGGGGAGCAGATGTGAACTGCACTCATGGCACACTGAAGCCCTTGCACTGTGCC
NM_024095.3         ATCAGAGGGGGAGCAGATGTGAACTGCACTCATGGCACACTGAAGCCCTTGCACTGTGCC
XM_005268654.1      ATCAGAGGGGGAGCAGATGTGAACTGCACTCATGGCACACTGAAGCCCTTGCACTGTGCC
                    ************************************************************

HsCD00287938        TGTATGGTGTCAGATGCTGACTGTGTGGAGTTACTTCTGGAAAAAGGAGCCGAGGTGAAT
NM_024095.3         TGTATGGTGTCAGATGCTGACTGTGTGGAGTTACTTCTGGAAAAAGGAGCCGAGGTGAAT
XM_005268654.1      TGTATGGTGTCAGATGCTGACTGTGTGGAGTTACTTCTGGAAAAAGGAGCCGAGGTGAAT
                    ************************************************************

HsCD00287938        GCCCTGGATGGGTATAACCGAACAGCCCTCCACTATGCAGCAGAGAAAGATGAGGCTTGT
NM_024095.3         GCCCTGGATGGGTATAACCGAACAGCCCTCCACTATGCAGCAGAGAAAGATGAGGCTTGT
XM_005268654.1      GCCCTGGATGGGTATAACCGAACAGCCCTCCACTATGCAGCAGAGAAAGATGAGGCTTGT
                    ************************************************************

HsCD00287938        GTGGAGGTCCTATTGGAGTATGGTGCAAACCCCAATGCTTTGGATGGCAACAGAGATACC
NM_024095.3         GTGGAGGTCCTATTGGAGTATGGTGCAAACCCCAATGCTTTGGATGGCAACAGAGATACC
XM_005268654.1      GTGGAGGTCCTATTGGAGTATGGTGCAAACCCCAATGCTTTGGATGGCAACAGAGATACC
                    ************************************************************

HsCD00287938        CCACTTCACTGGGCAGCCTTTAAGAACAATGCTGAGTGTGTGCGGGCTCTCCTAGAGAGC
NM_024095.3         CCACTTCACTGGGCAGCCTTTAAGAACAATGCTGAGTGTGTGCGGGCTCTCCTAGAGAGC
XM_005268654.1      CCACTTCACTGGGCAGCCTTTAAGAACAATGCTGAGTGTGTGCGGGCTCTCCTAGAGAGC
                    ************************************************************

HsCD00287938        GGGGCCTCTGTCAATGCCCTGGATTACAACAATGATACACCGCTCAGCTGGGCTGCCATG
NM_024095.3         GGGGCCTCTGTCAATGCCCTGGATTACAACAATGATACACCGCTCAGCTGGGCTGCCATG
XM_005268654.1      GGGGCCTCTGTCAATGCCCTGGATTACAACAATGATACACCGCTCAGCTGGGCTGCCATG
                    ************************************************************

HsCD00287938        AAGGGAAATCTTGAGAGTGTCAGCATCCTTCTGGATTATGGCGCAGAGGTCAGAGTCATC
NM_024095.3         AAGGGAAATCTTGAGAGTGTCAGCATCCTTCTGGATTATGGCGCAGAGGTCAGAGTCATC
XM_005268654.1      AAGGGAAATCTTGAGAGTGTCAGCATCCTTCTGGATTATGGCGCAGAGGTCAGAGTCATC
                    ************************************************************

HsCD00287938        AACCTAATAGGCCAGACACCCATCTCCCGCCTGGTGGCTCTGCTAGTCAGGGGACTTGGA
NM_024095.3         AACCTAATAGGCCAGACACCCATCTCCCGCCTGGTGGCTCTGCTAGTCAGGGGACTTGGA
XM_005268654.1      AACCTAATAGGCCAGACACCCATCTCCCGCCTGGTGGCTCTGCTAGTCAGGGGACTTGGA
                    ************************************************************

HsCD00287938        ACAGAGAAAGAGGACTCTTGCTTTGAGCTCCTCCACAGAGCTGTTGGACACTTTGAATTG
NM_024095.3         ACAGAGAAAGAGGACTCTTGCTTTGAGCTCCTCCACAGAGCTGTTGGACACTTTGAATTG
XM_005268654.1      ACAGAGAAAGAGGACTCTTGCTTTGAGCTCCTCCACAGAGCTGTTGGACACTTTGAATTG
                    ************************************************************

HsCD00287938        AGGAAAAATGGCACCATGCCACGAGAGGTGGCCAGAGACCCGCAGCTATGTGAAAAACTG
NM_024095.3         AGGAAAAATGGCACCATGCCACGAGAGGTGGCCAGAGACCCGCAGCTATGTGAAAAACTG
XM_005268654.1      AGGAAAAATGGCACCATGCCACGAGAGGTGGCCAGAGACCCGCAGCTATGTGAAAAACTG
                    ************************************************************

HsCD00287938        ACTGTTCTGTGCTCAGCTCCAGGAACTCTAAAAACACTCGCTCGCTATGCCGTGCGCCGT
NM_024095.3         ACTGTTCTGTGCTCAGCTCCAGGAACTCTAAAAACACTCGCTCGCTATGCCGTGCGCCGT
XM_005268654.1      ACTGTTCTGTGCTCAGCTCCAGGAACTCTAAAAACACTCGCTCGCTATGCCGTGCGCCGT
                    ************************************************************

HsCD00287938        AGCCTGGGACTCCAGTATCTCCCCGATGCAGTGAAGGGCCTTCCACTGCCAGCTTCTTTG
NM_024095.3         AGCCTGGGACTCCAGTATCTCCCCGATGCAGTGAAGGGCCTTCCACTGCCAGCTTCTTTG
XM_005268654.1      AGCCTGGGACTCCAGTATCTCCCCGATGCAGTGAAGGGCCTTCCACTGCCAGCTTCTTTG
                    ************************************************************

HsCD00287938        AAGGAATACCTGTTACTTTTAGAATAC
NM_024095.3         AAGGAATACCTGTTACTTTTAGAATAG
XM_005268654.1      AAGGAATACCTGTTACTTTTAGAATAG
                    **************************