DNASU Plasmid | PEX7 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: PEX7

Gene Name: peroxisomal biogenesis factor 7

Original Clone ID:

54562

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

None

Comments:

None

Mutation / Discrepancy:

No / Yes

Publications:

None

Authors:

The ORFeome Collaboration

HsCD00288110

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 972nts

Open reading frame: 1 to 972

Reference Sequence Alignment

ATGAGTGCGGTGTGCGGTGGAGCGGCGCGGATGCTGCGGACGCCGGGACGCCACGGCTAC
GCCGCCGAGTTCTCCCCGTACCTGCCGGGCCGCCTGGCCTGCGCCACCGCGCAGCACTAC
GGCATCGCGGGCTGTGGAACCCTACTAATATTGGATCCAGATGAAGCTGGGCTAAGGCTT
TTTAGAAGCTTTGACTGGAATGATGGTTTGTTTGATGTGACTTGGAGTGAGAACAACGAA
CATGTCCTCATCACCTGTAGTGGCGATGGCTCGCTGCAGCTCTGGGACACTGCCAAAGCT
GCAGGGCCACTGCAAGTCTATAAAGAACACGCTCAGGAGGTGTATAGTGTTGATTGGAGC
CAAACCAGAGGTGAACAGCTTGTGGTGTCTGGCTCATGGGATCAAACTGTCAAATTGTGG
GATCCAACTGTTGGAAAGTCTCTGTGCACCTTTAGAGGCCATGAAAGTATTATTTATAGC
ACAATCTGGTCTCCCCACATCCCTGGTTGTTTTGCTTCAGCCTCAGGTGATCAGACTCTG
AGAATATGGGATGTGAAGGCAGCAGGAGTAAGAATCGTGATTCCTGCACATCAGGCAGAA
ATCTTGAGTTGTGACTGGTGTAAATACAATGAGAATTTGCTGGTGACCGGGGCGGTTGAC
TGTAGTTTGAGAGGCTGGGACTTAAGGAATGTACGGCAACCAGTGTTTGAACTTCTTGGT
CATACCTATGCTATTAGGAGGGTGAAATTTTCACCATTTCATGCTTCTGTGCTGGCCTCT
TGCTCGTATGATTTTACTGTAAGATTCTGGAACTTTTCAAAGCCTGACTCTCTTCTTGAA
ACAGTGGAGCATCATACAGAGTTTACTTGTGGTTTAGACTTCAGTCTTCAGAGCCCCACT
CAGGTGGCTGACTGTTCTTGGGATGAAACAATAAAGATCTATGACCCTGCTTGTCTTACT
ATTCCTGCTTGC

Protein sequence predicted based on nucleotide sequence

MSAVCGGAARMLRTPGRHGYAAEFSPYLPGRLACATAQHYGIAGCGTLLILDPDEAGLRL
FRSFDWNDGLFDVTWSENNEHVLITCSGDGSLQLWDTAKAAGPLQVYKEHAQEVYSVDWS
QTRGEQLVVSGSWDQTVKLWDPTVGKSLCTFRGHESIIYSTIWSPHIPGCFASASGDQTL
RIWDVKAAGVRIVIPAHQAEILSCDWCKYNENLLVTGAVDCSLRGWDLRNVRQPVFELLG
HTYAIRRVKFSPFHASVLASCSYDFTVRFWNFSKPDSLLETVEHHTEFTCGLDFSLQSPT
QVADCSWDETIKIYDPACLTIPAC

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Reference Sequence Annotations:

End on reference sequence 1016

Start on reference sequence 45

Mutations:

None

Discrepancy:

a696g

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

PEX7

Symbol Nomenclature :

PEX7

SYNONYM :

PBD9B; PTS2R; RCDP1; RD

Designation :

PTS2 receptor|peroxin-7|peroxisomal PTS2 receptor|peroxisomal targeting signal 2 receptor|peroxisome targeting signal 2 receptor

Full Nomenclature :

peroxisomal biogenesis factor 7

GENEID :

5191

Locus Tag :

RP11-55K22.6

HGNC :

8860

MIM :

601757

Vega :

OTTHUMG00000015650

Target GenBank:

BC006268.1

Reference Sequence Alignment

  

HsCD00288110      ATGAGTGCGGTGTGCGGTGGAGCGGCGCGGATGCTGCGGACGCCGGGACGCCACGGCTAC
NM_000288.3       ATGAGTGCGGTGTGCGGTGGAGCGGCGCGGATGCTGCGGACGCCGGGACGCCACGGCTAC
                  ************************************************************

HsCD00288110      GCCGCCGAGTTCTCCCCGTACCTGCCGGGCCGCCTGGCCTGCGCCACCGCGCAGCACTAC
NM_000288.3       GCCGCCGAGTTCTCCCCGTACCTGCCGGGCCGCCTGGCCTGCGCCACCGCGCAGCACTAC
                  ************************************************************

HsCD00288110      GGCATCGCGGGCTGTGGAACCCTACTAATATTGGATCCAGATGAAGCTGGGCTAAGGCTT
NM_000288.3       GGCATCGCGGGCTGTGGAACCCTACTAATATTGGATCCAGATGAAGCTGGGCTAAGGCTT
                  ************************************************************

HsCD00288110      TTTAGAAGCTTTGACTGGAATGATGGTTTGTTTGATGTGACTTGGAGTGAGAACAACGAA
NM_000288.3       TTTAGAAGCTTTGACTGGAATGATGGTTTGTTTGATGTGACTTGGAGTGAGAACAACGAA
                  ************************************************************

HsCD00288110      CATGTCCTCATCACCTGTAGTGGCGATGGCTCGCTGCAGCTCTGGGACACTGCCAAAGCT
NM_000288.3       CATGTCCTCATCACCTGTAGTGGCGATGGCTCGCTGCAGCTCTGGGACACTGCCAAAGCT
                  ************************************************************

HsCD00288110      GCAGGGCCACTGCAAGTCTATAAAGAACACGCTCAGGAGGTGTATAGTGTTGATTGGAGC
NM_000288.3       GCAGGGCCACTGCAAGTCTATAAAGAACACGCTCAGGAGGTGTATAGTGTTGATTGGAGC
                  ************************************************************

HsCD00288110      CAAACCAGAGGTGAACAGCTTGTGGTGTCTGGCTCATGGGATCAAACTGTCAAATTGTGG
NM_000288.3       CAAACCAGAGGTGAACAGCTTGTGGTGTCTGGCTCATGGGATCAAACTGTCAAATTGTGG
                  ************************************************************

HsCD00288110      GATCCAACTGTTGGAAAGTCTCTGTGCACCTTTAGAGGCCATGAAAGTATTATTTATAGC
NM_000288.3       GATCCAACTGTTGGAAAGTCTCTGTGCACCTTTAGAGGCCATGAAAGTATTATTTATAGC
                  ************************************************************

HsCD00288110      ACAATCTGGTCTCCCCACATCCCTGGTTGTTTTGCTTCAGCCTCAGGTGATCAGACTCTG
NM_000288.3       ACAATCTGGTCTCCCCACATCCCTGGTTGTTTTGCTTCAGCCTCAGGTGATCAGACTCTG
                  ************************************************************

HsCD00288110      AGAATATGGGATGTGAAGGCAGCAGGAGTAAGAATCGTGATTCCTGCACATCAGGCAGAA
NM_000288.3       AGAATATGGGATGTGAAGGCAGCAGGAGTAAGAATCGTGATTCCTGCACATCAGGCAGAA
                  ************************************************************

HsCD00288110      ATCTTGAGTTGTGACTGGTGTAAATACAATGAGAATTTGCTGGTGACCGGGGCGGTTGAC
NM_000288.3       ATCTTGAGTTGTGACTGGTGTAAATACAATGAGAATTTGCTGGTGACCGGGGCGGTTGAC
                  ************************************************************

HsCD00288110      TGTAGTTTGAGAGGCTGGGACTTAAGGAATGTACGGCAACCAGTGTTTGAACTTCTTGGT
NM_000288.3       TGTAGTTTGAGAGGCTGGGACTTAAGGAATGTACGACAACCAGTGTTTGAACTTCTTGGT
                  ***********************************.************************

HsCD00288110      CATACCTATGCTATTAGGAGGGTGAAATTTTCACCATTTCATGCTTCTGTGCTGGCCTCT
NM_000288.3       CATACCTATGCTATTAGGAGGGTGAAATTTTCACCATTTCATGCTTCTGTGCTGGCCTCT
                  ************************************************************

HsCD00288110      TGCTCGTATGATTTTACTGTAAGATTCTGGAACTTTTCAAAGCCTGACTCTCTTCTTGAA
NM_000288.3       TGCTCGTATGATTTTACTGTAAGATTCTGGAACTTTTCAAAGCCTGACTCTCTTCTTGAA
                  ************************************************************

HsCD00288110      ACAGTGGAGCATCATACAGAGTTTACTTGTGGTTTAGACTTCAGTCTTCAGAGCCCCACT
NM_000288.3       ACAGTGGAGCATCATACAGAGTTTACTTGTGGTTTAGACTTCAGTCTTCAGAGCCCCACT
                  ************************************************************

HsCD00288110      CAGGTGGCTGACTGTTCTTGGGATGAAACAATAAAGATCTATGACCCTGCTTGTCTTACT
NM_000288.3       CAGGTGGCTGACTGTTCTTGGGATGAAACAATAAAGATCTATGACCCTGCTTGTCTTACT
                  ************************************************************

HsCD00288110      ATTCCTGCTTGC
NM_000288.3       ATTCCTGCTTGA
                  ***********.