DNASU Plasmid | TTC5 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: TTC5

Gene Name: tetratricopeptide repeat domain 5

Original Clone ID:

39281

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

The ORFeome Collaboration

HsCD00288243

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1323nts

Open reading frame: 1 to 1323

Reference Sequence Alignment

ATGATGGCTGATGAAGAGGAAGAAGTCAAGCCGATCTTGCAGAAATTGCAGGAACTCGTG
GATCAGCTCTACTCATTTCGAGACTGCTATTTCGAGACACATAGTGTTGAGGATGCTGGG
AGGAAGCAACAGGATGTGCGGAAGGAGATGGAGAAAACCCTACAGCAGATGGAAGAAGTA
GTGGGTTCTGTCCAGGGCAAGGCACAAGTTCTAATGCTAACTGGGAAAGCACTAAATGTG
ACTCCTGACTATAGCCCTAAGGCTGAGGAGCTTCTGTCAAAGGCTGTGAAGCTGGAGCCC
GAGCTGGTGGAAGCCTGGAACCAGCTGGGTGAGGTGTACTGGAAAAAAGGGGATGTTGCA
GCTGCCCACACCTGCTTCTCAGGAGCCCTCACCCATTGCAGGAACAAAGTCTCCTTGCAA
AACCTGTCAATGGTGCTTCGTCAGCTGCGGACTGACACTGAAGATGAACATTCTCACCAT
GTCATGGACAGTGTCCGACAGGCTAAGTTGGCTGTTCAGATGGATGTCCATGATGGCCGC
TCCTGGTATATTCTTGGGAATTCATATCTTTCCCTTTACTTCTCTACTGGCCAGAACCCT
AAGATCTCCCAGCAAGCCCTCAGTGCCTATGCCCAAGCAGAGAAAGTTGACAGAAAAGCT
TCTAGCAATCCTGACCTTCATCTGAACAGGGCGACGTTGCATAAATATGAAGAGAGTTAT
GGGGAGGCCCTGGAGGGCTTCTCTCGGGCTGCAGCCCTGGACCCTGCCTGGCCAGAGCCC
CGGCAACGAGAGCAACAACTTCTGGAATTCCTGGATAGATTAACCAGCCTCCTTGAGAGT
AAGGGAAAGGTGAAGACCAAAAAGCTGCAGAGCATGCTGGGAAGCTTGCGCCCAGCCCAT
CTAGGCCCTTGCAGTGATGGGCACTATCAGTCAGCCTCTGGGCAGAAAGTGACCCTGGAG
CTCAAGCCACTGAGTACGCTTCAGCCTGGGGTGAACAGCGGTGCCGTCATCCTGGGAAAG
GTGGTATTTAGCCTCACCACAGAGGAGAAAGTCCCCTTTACATTTGGCCTGGTAGATTCA
GATGGACCTTGCTATGCAGTGATGGTGTACAATATAGTGCAGAGCTGGGGAGTGCTCATT
GGAGACTCTGTAGCCATTCCTGAGCCCAACCTGCGGCTTCACCGAATTCAGCACAAAGGA
AAGGACTATTCCTTTTCCAGTGTTCGAGTGGAGACGCCCCTCCTGCTAGTGGTGAATGGG
AAGCCTCAGGGATCCAGCAGCCAGGCTGTTGCCACAGTGGCATCGCGACCACAGTGTGAA
TGC

Protein sequence predicted based on nucleotide sequence

MMADEEEEVKPILQKLQELVDQLYSFRDCYFETHSVEDAGRKQQDVRKEMEKTLQQMEEV
VGSVQGKAQVLMLTGKALNVTPDYSPKAEELLSKAVKLEPELVEAWNQLGEVYWKKGDVA
AAHTCFSGALTHCRNKVSLQNLSMVLRQLRTDTEDEHSHHVMDSVRQAKLAVQMDVHDGR
SWYILGNSYLSLYFSTGQNPKISQQALSAYAQAEKVDRKASSNPDLHLNRATLHKYEESY
GEALEGFSRAAALDPAWPEPRQREQQLLEFLDRLTSLLESKGKVKTKKLQSMLGSLRPAH
LGPCSDGHYQSASGQKVTLELKPLSTLQPGVNSGAVILGKVVFSLTTEEKVPFTFGLVDS
DGPCYAVMVYNIVQSWGVLIGDSVAIPEPNLRLHRIQHKGKDYSFSSVRVETPLLLVVNG
KPQGSSSQAVATVASRPQCEC

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Reference Sequence Annotations:

End on reference sequence 1328

Start on reference sequence 6

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

TTC5

Symbol Nomenclature :

TTC5

SYNONYM :

Strap

Designation :

TPR repeat protein 5|stress-responsive activator of p300|tetratricopeptide repeat protein 5

Full Nomenclature :

tetratricopeptide repeat domain 5

GENEID :

91875

HGNC :

19274

Target GenBank:

BC053538.1

Reference Sequence Alignment

  

HsCD00288243      ATGATGGCTGATGAAGAGGAAGAAGTCAAGCCGATCTTGCAGAAATTGCAGGAACTCGTG
NM_138376.2       ATGATGGCTGATGAAGAGGAAGAAGTCAAGCCGATCTTGCAGAAATTGCAGGAACTCGTG
                  ************************************************************

HsCD00288243      GATCAGCTCTACTCATTTCGAGACTGCTATTTCGAGACACATAGTGTTGAGGATGCTGGG
NM_138376.2       GATCAGCTCTACTCATTTCGAGACTGCTATTTCGAGACACATAGTGTTGAGGATGCTGGG
                  ************************************************************

HsCD00288243      AGGAAGCAACAGGATGTGCGGAAGGAGATGGAGAAAACCCTACAGCAGATGGAAGAAGTA
NM_138376.2       AGGAAGCAACAGGATGTGCAGAAGGAGATGGAGAAAACCCTACAGCAGATGGAAGAAGTA
                  *******************.****************************************

HsCD00288243      GTGGGTTCTGTCCAGGGCAAGGCACAAGTTCTAATGCTAACTGGGAAAGCACTAAATGTG
NM_138376.2       GTGGGTTCTGTCCAGGGCAAGGCACAAGTTCTAATGCTAACTGGGAAAGCACTAAATGTG
                  ************************************************************

HsCD00288243      ACTCCTGACTATAGCCCTAAGGCTGAGGAGCTTCTGTCAAAGGCTGTGAAGCTGGAGCCC
NM_138376.2       ACTCCTGACTATAGCCCTAAGGCTGAGGAGCTTCTGTCAAAGGCTGTGAAGCTGGAGCCC
                  ************************************************************

HsCD00288243      GAGCTGGTGGAAGCCTGGAACCAGCTGGGTGAGGTGTACTGGAAAAAAGGGGATGTTGCA
NM_138376.2       GAGCTGGTGGAAGCCTGGAACCAGCTGGGTGAGGTGTACTGGAAAAAAGGGGATGTTGCA
                  ************************************************************

HsCD00288243      GCTGCCCACACCTGCTTCTCAGGAGCCCTCACCCATTGCAGGAACAAAGTCTCCTTGCAA
NM_138376.2       GCTGCCCACACCTGCTTCTCAGGAGCCCTCACCCATTGCAGGAACAAAGTCTCCTTGCAA
                  ************************************************************

HsCD00288243      AACCTGTCAATGGTGCTTCGTCAGCTGCGGACTGACACTGAAGATGAACATTCTCACCAT
NM_138376.2       AACCTGTCAATGGTGCTTCGTCAGCTGCGGACTGACACTGAAGATGAACATTCTCACCAT
                  ************************************************************

HsCD00288243      GTCATGGACAGTGTCCGACAGGCTAAGTTGGCTGTTCAGATGGATGTCCATGATGGCCGC
NM_138376.2       GTCATGGACAGTGTCCGACAGGCTAAGTTGGCTGTTCAGATGGATGTCCATGATGGCCGC
                  ************************************************************

HsCD00288243      TCCTGGTATATTCTTGGGAATTCATATCTTTCCCTTTACTTCTCTACTGGCCAGAACCCT
NM_138376.2       TCCTGGTATATTCTTGGGAATTCATATCTTTCCCTTTACTTCTCTACTGGCCAGAACCCT
                  ************************************************************

HsCD00288243      AAGATCTCCCAGCAAGCCCTCAGTGCCTATGCCCAAGCAGAGAAAGTTGACAGAAAAGCT
NM_138376.2       AAGATCTCCCAGCAAGCCCTCAGTGCCTATGCCCAAGCAGAGAAAGTTGACAGAAAAGCT
                  ************************************************************

HsCD00288243      TCTAGCAATCCTGACCTTCATCTGAACAGGGCGACGTTGCATAAATATGAAGAGAGTTAT
NM_138376.2       TCTAGCAATCCTGACCTTCATCTGAACAGGGCGACGTTGCATAAATATGAAGAGAGTTAT
                  ************************************************************

HsCD00288243      GGGGAGGCCCTGGAGGGCTTCTCTCGGGCTGCAGCCCTGGACCCTGCCTGGCCAGAGCCC
NM_138376.2       GGGGAGGCCCTGGAGGGCTTCTCTCGGGCTGCAGCCCTGGACCCTGCCTGGCCAGAGCCC
                  ************************************************************

HsCD00288243      CGGCAACGAGAGCAACAACTTCTGGAATTCCTGGATAGATTAACCAGCCTCCTTGAGAGT
NM_138376.2       CGGCAACGAGAGCAACAACTTCTGGAATTCCTGGATAGATTAACCAGCCTCCTTGAGAGT
                  ************************************************************

HsCD00288243      AAGGGAAAGGTGAAGACCAAAAAGCTGCAGAGCATGCTGGGAAGCTTGCGCCCAGCCCAT
NM_138376.2       AAGGGAAAGGTGAAGACCAAAAAGCTGCAGAGCATGCTGGGAAGCTTGCGCCCAGCCCAT
                  ************************************************************

HsCD00288243      CTAGGCCCTTGCAGTGATGGGCACTATCAGTCAGCCTCTGGGCAGAAAGTGACCCTGGAG
NM_138376.2       CTAGGCCCTTGCAGTGATGGGCACTATCAGTCAGCCTCTGGGCAGAAAGTGACCCTGGAG
                  ************************************************************

HsCD00288243      CTCAAGCCACTGAGTACGCTTCAGCCTGGGGTGAACAGCGGTGCCGTCATCCTGGGAAAG
NM_138376.2       CTCAAGCCACTGAGTACGCTTCAGCCTGGGGTGAACAGCGGTGCCGTCATCCTGGGAAAG
                  ************************************************************

HsCD00288243      GTGGTATTTAGCCTCACCACAGAGGAGAAAGTCCCCTTTACATTTGGCCTGGTAGATTCA
NM_138376.2       GTGGTATTTAGCCTCACCACAGAGGAGAAAGTCCCCTTTACATTTGGCCTGGTAGATTCA
                  ************************************************************

HsCD00288243      GATGGACCTTGCTATGCAGTGATGGTGTACAATATAGTGCAGAGCTGGGGAGTGCTCATT
NM_138376.2       GATGGACCTTGCTATGCAGTGATGGTGTACAATATAGTGCAGAGCTGGGGAGTGCTCATT
                  ************************************************************

HsCD00288243      GGAGACTCTGTAGCCATTCCTGAGCCCAACCTGCGGCTTCACCGAATTCAGCACAAAGGA
NM_138376.2       GGAGACTCTGTAGCCATTCCTGAGCCCAACCTGCGGCTTCACCGAATTCAGCACAAAGGA
                  ************************************************************

HsCD00288243      AAGGACTATTCCTTTTCCAGTGTTCGAGTGGAGACGCCCCTCCTGCTAGTGGTGAATGGG
NM_138376.2       AAGGACTATTCCTTTTCCAGTGTTCGAGTGGAGACGCCCCTCCTGCTAGTGGTGAATGGG
                  ************************************************************

HsCD00288243      AAGCCTCAGGGATCCAGCAGCCAGGCTGTTGCCACAGTGGCATCGCGACCACAGTGTGAA
NM_138376.2       AAGCCTCAGGGATCCAGCAGCCAGGCTGTTGCCACAGTGGCATCGCGACCACAGTGTGAA
                  ************************************************************

HsCD00288243      TGC
NM_138376.2       TGA
                  **.