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UPP2 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  UPP2
Gene Name:  uridine phosphorylase 2
Sequence             
Original Clone ID: 29821
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
HsCD00288342

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 954nts         Open reading frame : 1 to 954
Reference Sequence Alignment

ATGGCTTCAGTTATACCTGCCTCCAATAGGTCCATGAGATCTGACAGGAATACATATGTT
GGAAAAAGGTTTGTTCACGTTAAAAATCCTTACTTGGATTTGATGGATGAAGACATTCTC
TATCACTTGGATTTGGGAACAAAAACACACAACCTACCAGCAATGTTTGGAGATGTAAAG
TTTGTCTGTGTCGGTGGGAGCCCCAACAGAATGAAAGCATTTGCACTGTTTATGCACAAG
GAGCTCGGGTTTGAGGAAGCTGAAGAAGACATAAAAGACATCTGTGCTGGGACAGACAGA
TACTGTATGTACAAAACCGGGCCTGTGCTCGCCATCAGTCACGGCATGGGCATCCCCTCC
ATTTCTATTATGCTTCATGAACTCATCAAATTACTCCACCATGCACGGTGCTGCGATGTC
ACCATTATTAGAATCGGTACATCAGGGGGAATAGGGATTGCACCAGGGACTGTTGTAATA
ACGGATATAGCTGTAGACTCCTTCTTTAAGCCCCGGTTTGAACAGGTCATTTTGGACAAC
ATTGTCACCCGAAGTACTGAACTGGACAAAGAACTGTCTGAAGAACTGTTCAACTGTAGC
AAAGAAATCCCCAACTTCCCAACCCTCGTTGGACATACAATGTGTACCTATGATTTTTAT
GAAGGCCAAGGCCGACTAGATGGAGCACTGTGCTCCTTTTCCAGAGAAAAAAAGTTAGAC
TACTTGAAGAGAGCATTTAAAGCTGGTGTCAGGAATATTGAAATGGAATCTACAGTGTTT
GCAGCTATGTGTGGACTCTGTGGTCTAAAAGCTGCTGTGGTCTGTGTGACACTTCTCGAC
AGACTCGACTGTGATCAGATCAACTTGCCTCATGATGTCCTGGTGGAGTACCAGCAACGG
CCTCAGCTCCTAATCTCCAACTTCATCAGACGGCGGCTTGGACTTTGTGACTAC


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1145
Start on reference sequence 192
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
GTACAAAAAAGTTGGC

3' Linker Sequence:
5p (goi-stop)CCAACTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223

pENTR223 in advanced viewed

Synonyms: pDONR223, pENTR223-XnCDSYn
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp): 2815
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Mark Vidal
Publications: PMID: 15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 2220 2753
gene fragment pas 5p stem of pBR primose assembly site 63 73
gene fragment pas prime 3p stem of pBR pas(L) 105 116
recombination site attL1 attL1 recombination site 570 665
recombination site attL2 attL2 recombination site 793 696
selectable marker SpnR spectinomycin resistance gene 1062 2072
trxn termination sequence T1-T2 rrnBT1 and T2 terminators 510 224

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : UPP2
Symbol Nomenclature : UPP2
SYNONYM : UDRPASE2; UP2; UPASE2
Designation : UPase 2|liver-specific uridine phosphorylase|urdPase 2|uridine phosphorylase-2
Full Nomenclature : uridine phosphorylase 2
GENEID : 151531
HGNC : 23061
Vega : OTTHUMG00000131969
Target GenBank: BC033529.2

 

Reference Sequence Alignment

  

HsCD00288342        ------------------------------------------------------------
NM_173355.3         ------------------------------------------------------------
NM_001135098.1      ATGCTGGCCCCAGGCTGTGAGTTGGACCCAGACCAAGAAGTGGTGAGGACAAGGCCTGAA
                                                                                

HsCD00288342        ------------------------------------------------------------
NM_173355.3         ------------------------------------------------------------
NM_001135098.1      GATGTGCCTGCTTCCCCTTCAACTTCCACCATGATTGTAAGTGTCCTGAGGCCTCCCAGC
                                                                                

HsCD00288342        ---------------------------------------------------ATGGCTTCA
NM_173355.3         ---------------------------------------------------ATGGCTTCA
NM_001135098.1      CATGCTTCCTGTACAGCCTGTGGAACTGTGACTTTTCACATAGTAGAGAGAATGGCTTCA
                                                                       *********

HsCD00288342        GTTATACCTGCCTCCAATAGGTCCATGAGATCTGACAGGAATACATATGTTGGAAAAAGG
NM_173355.3         GTTATACCTGCCTCCAATAGGTCCATGAGATCTGACAGGAATACATATGTTGGAAAAAGG
NM_001135098.1      GTTATACCTGCCTCCAATAGGTCCATGAGATCTGACAGGAATACATATGTTGGAAAAAGG
                    ************************************************************

HsCD00288342        TTTGTTCACGTTAAAAATCCTTACTTGGATTTGATGGATGAAGACATTCTCTATCACTTG
NM_173355.3         TTTGTTCACGTTAAAAATCCTTACTTGGATTTGATGGATGAAGACATTCTCTATCACTTG
NM_001135098.1      TTTGTTCACGTTAAAAATCCTTACTTGGATTTGATGGATGAAGACATTCTCTATCACTTG
                    ************************************************************

HsCD00288342        GATTTGGGAACAAAAACACACAACCTACCAGCAATGTTTGGAGATGTAAAGTTTGTCTGT
NM_173355.3         GATTTGGGAACAAAAACACACAACCTACCAGCAATGTTTGGAGATGTAAAGTTTGTCTGT
NM_001135098.1      GATTTGGGAACAAAAACACACAACCTACCAGCAATGTTTGGAGATGTAAAGTTTGTCTGT
                    ************************************************************

HsCD00288342        GTCGGTGGGAGCCCCAACAGAATGAAAGCATTTGCACTGTTTATGCACAAGGAGCTCGGG
NM_173355.3         GTCGGTGGGAGCCCCAACAGAATGAAAGCATTTGCACTGTTTATGCACAAGGAGCTCGGG
NM_001135098.1      GTCGGTGGGAGCCCCAACAGAATGAAAGCATTTGCACTGTTTATGCACAAGGAGCTCGGG
                    ************************************************************

HsCD00288342        TTTGAGGAAGCTGAAGAAGACATAAAAGACATCTGTGCTGGGACAGACAGATACTGTATG
NM_173355.3         TTTGAGGAAGCTGAAGAAGACATAAAAGACATCTGTGCTGGGACAGACAGATACTGTATG
NM_001135098.1      TTTGAGGAAGCTGAAGAAGACATAAAAGACATCTGTGCTGGGACAGACAGATACTGTATG
                    ************************************************************

HsCD00288342        TACAAAACCGGGCCTGTGCTCGCCATCAGTCACGGCATGGGCATCCCCTCCATTTCTATT
NM_173355.3         TACAAAACCGGGCCTGTGCTCGCCATCAGTCACGGCATGGGCATCCCCTCCATTTCTATT
NM_001135098.1      TACAAAACCGGGCCTGTGCTCGCCATCAGTCACGGCATGGGCATCCCCTCCATTTCTATT
                    ************************************************************

HsCD00288342        ATGCTTCATGAACTCATCAAATTACTCCACCATGCACGGTGCTGCGATGTCACCATTATT
NM_173355.3         ATGCTTCATGAACTCATCAAATTACTCCACCATGCACGGTGCTGCGATGTCACCATTATT
NM_001135098.1      ATGCTTCATGAACTCATCAAATTACTCCACCATGCACGGTGCTGCGATGTCACCATTATT
                    ************************************************************

HsCD00288342        AGAATCGGTACATCAGGGGGAATAGGGATTGCACCAGGGACTGTTGTAATAACGGATATA
NM_173355.3         AGAATCGGTACATCAGGGGGAATAGGGATTGCACCAGGGACTGTTGTAATAACGGATATA
NM_001135098.1      AGAATCGGTACATCAGGGGGAATAGGGATTGCACCAGGGACTGTTGTAATAACGGATATA
                    ************************************************************

HsCD00288342        GCTGTAGACTCCTTCTTTAAGCCCCGGTTTGAACAGGTCATTTTGGACAACATTGTCACC
NM_173355.3         GCTGTAGACTCCTTCTTTAAGCCCCGGTTTGAACAGGTCATTTTGGACAACATTGTCACC
NM_001135098.1      GCTGTAGACTCCTTCTTTAAGCCCCGGTTTGAACAGGTCATTTTGGACAACATTGTCACC
                    ************************************************************

HsCD00288342        CGAAGTACTGAACTGGACAAAGAACTGTCTGAAGAACTGTTCAACTGTAGCAAAGAAATC
NM_173355.3         CGAAGTACTGAACTGGACAAAGAACTGTCTGAAGAACTGTTCAACTGTAGCAAAGAAATC
NM_001135098.1      CGAAGTACTGAACTGGACAAAGAACTGTCTGAAGAACTGTTCAACTGTAGCAAAGAAATC
                    ************************************************************

HsCD00288342        CCCAACTTCCCAACCCTCGTTGGACATACAATGTGTACCTATGATTTTTATGAAGGCCAA
NM_173355.3         CCCAACTTCCCAACCCTCGTTGGACATACAATGTGTACCTATGATTTTTATGAAGGCCAA
NM_001135098.1      CCCAACTTCCCAACCCTCGTTGGACATACAATGTGTACCTATGATTTTTATGAAGGCCAA
                    ************************************************************

HsCD00288342        GGCCGACTAGATGGAGCACTGTGCTCCTTTTCCAGAGAAAAAAAGTTAGACTACTTGAAG
NM_173355.3         GGCCGACTAGATGGAGCACTGTGCTCCTTTTCCAGAGAAAAAAAGTTAGACTACTTGAAG
NM_001135098.1      GGCCGACTAGATGGAGCACTGTGCTCCTTTTCCAGAGAAAAAAAGTTAGACTACTTGAAG
                    ************************************************************

HsCD00288342        AGAGCATTTAAAGCTGGTGTCAGGAATATTGAAATGGAATCTACAGTGTTTGCAGCTATG
NM_173355.3         AGAGCATTTAAAGCTGGTGTCAGGAATATTGAAATGGAATCTACAGTGTTTGCAGCTATG
NM_001135098.1      AGAGCATTTAAAGCTGGTGTCAGGAATATTGAAATGGAATCTACAGTGTTTGCAGCTATG
                    ************************************************************

HsCD00288342        TGTGGACTCTGTGGTCTAAAAGCTGCTGTGGTCTGTGTGACACTTCTCGACAGACTCGAC
NM_173355.3         TGTGGACTCTGTGGTCTAAAAGCTGCTGTGGTCTGTGTGACACTTCTCGACAGACTCGAC
NM_001135098.1      TGTGGACTCTGTGGTCTAAAAGCTGCTGTGGTCTGTGTGACACTTCTCGACAGACTCGAC
                    ************************************************************

HsCD00288342        TGTGATCAGATCAACTTGCCTCATGATGTCCTGGTGGAGTACCAGCAACGGCCTCAGCTC
NM_173355.3         TGTGATCAGATCAACTTGCCTCATGATGTCCTGGTGGAGTACCAGCAACGGCCTCAGCTC
NM_001135098.1      TGTGATCAGATCAACTTGCCTCATGATGTCCTGGTGGAGTACCAGCAACGGCCTCAGCTC
                    ************************************************************

HsCD00288342        CTAATCTCCAACTTCATCAGACGGCGGCTTGGACTTTGTGACTAC
NM_173355.3         CTAATCTCCAACTTCATCAGACGGCGGCTTGGACTTTGTGACTAG
NM_001135098.1      CTAATCTCCAACTTCATCAGACGGCGGCTTGGACTTTGTGACTAG
                    ******************************************** 

Pathways

Panther : Salvage pyrimidine ribonucleotides
Reactome : Metabolism
Reactome : Metabolism of nucleotides
Reactome : Pyrimidine catabolism
Reactome : Pyrimidine metabolism
Reactome : Pyrimidine salvage reactions
WikiPathway : Fluoropyrimidine Activity

Protein

UniProt : O95045
HPRD : 15627

Clone

Cloning Information : 471579
HIP Master Clone ID : 394497
Original Clone ID : 29821

Organism


TAX_ID : 9606
Species Specific ID: 151531

Genome

Chromosome : 2
Map Location : 2q24.1
Ensembl : ENSG00000007001

Reagents

Labome : UPP2-antibody