DNASU Plasmid | ZNF774 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: ZNF774

Gene Name: zinc finger protein 774

Original Clone ID:

56141

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

The ORFeome Collaboration

HsCD00288694

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1452nts

Open reading frame: 1 to 1452

Reference Sequence Alignment

ATGTGGCTGGGGACTTCAGGGAAGAGTGGGTTACCTGGACACTGCTTAGAGAATCCTCTC
CAGGAATGCCACCCAGCACAGTTAGAAGAATGGGCTCTCAAAGGAATTTCCAGGCCTAGT
GTAATCTCCCAGCCGGAGCAGAAAGAAGAGCCATGGGTCCTACCACTCCAAAACTTTGAG
GCGAGGAAGATCCCGAGGGAAAGCCACACAGACTGTGAGCATCAGGTGGCAAAGCTCAAT
CAGGACAGTTCTGAAACAGCAGAACAATGTGGAACATCCTCAGAAAGGACCAATAAAGAT
CTTTCTCATACTCTTAGTTGGGGAGGAAACTGGGAGCAAGGCCTAGAATTAGAAGGGCAA
CATGGAACCCTTCCAGGAGAGGGCCAGCTGGAGTCCTTTTCACAGGAGAGGGATTTAAAC
AAGCTCCTGGATGGATATGTAGGAGAGAAGCCTATGTGTGCAGAATGCGGGAAAAGCTTT
AACCAGAGTTCCTATCTCATAAGACACCTAAGAACCCACACTGGCGAGAGGCCCTATACG
TGCATTGAGTGTGGGAAAGGCTTCAAACAGAGCTCAGACCTTGTCACCCATCGCAGAACA
CACACAGGAGAGAAGCCCTACCAATGCAAGGGGTGTGAGAAGAAATTCAGCGACAGCTCA
ACACTCATCAAACATCAGAGAACCCACACAGGGGAGAGACCCTATGAGTGCCCAGAGTGT
GGAAAGACTTTTGGGCGGAAGCCACACCTCATAATGCACCAAAGAACCCACACAGGCGAG
AAGCCCTACGCGTGCCTGGAATGTCACAAAAGCTTCAGTCGAAGCTCAAATTTCATCACT
CACCAGAGGACCCACACAGGGGTGAAGCCTTACAGGTGTAATGACTGTGGGGAGAGTTTT
AGCCAGAGCTCGGATTTGATTAAGCACCAACGAACCCACACGGGAGAACGGCCCTTCAAA
TGCCCGGAGTGCGGGAAGGGCTTCAGAGATAGTTCTCATTTTGTAGCTCACATGAGCACT
CATTCAGGAGAGAGGCCTTTCAGTTGTCCTGACTGCCACAAAAGCTTCAGTCAGAGCTCA
CATTTGGTCACGCACCAAAGAACACACACAGGTGAGAGACCTTTTAAGTGCGAAAACTGT
GGGAAAGGATTCGCCGACAGCTCCGCCCTCATTAAGCACCAACGAATCCACACCGGAGAA
AGACCCTACAAATGTGGAGAGTGTGGGAAGAGCTTCAATCAGAGCTCCCACTTTATTACC
CATCAGCGAATCCACTTAGGAGACAGGCCCTATCGATGTCCTGAGTGTGGCAAGACCTTC
AATCAGCGTTCCCATTTCCTCACACACCAGAGAACGCATACAGGAGAAAAACCTTTCCAC
TGTAGTAAATGTAACAAGAGCTTCCGTCAGAAAGCGCATCTTTTATGCCATCAAAACACC
CATTTGATTTAC

Protein sequence predicted based on nucleotide sequence

MWLGTSGKSGLPGHCLENPLQECHPAQLEEWALKGISRPSVISQPEQKEEPWVLPLQNFE
ARKIPRESHTDCEHQVAKLNQDSSETAEQCGTSSERTNKDLSHTLSWGGNWEQGLELEGQ
HGTLPGEGQLESFSQERDLNKLLDGYVGEKPMCAECGKSFNQSSYLIRHLRTHTGERPYT
CIECGKGFKQSSDLVTHRRTHTGEKPYQCKGCEKKFSDSSTLIKHQRTHTGERPYECPEC
GKTFGRKPHLIMHQRTHTGEKPYACLECHKSFSRSSNFITHQRTHTGVKPYRCNDCGESF
SQSSDLIKHQRTHTGERPFKCPECGKGFRDSSHFVAHMSTHSGERPFSCPDCHKSFSQSS
HLVTHQRTHTGERPFKCENCGKGFADSSALIKHQRIHTGERPYKCGECGKSFNQSSHFIT
HQRIHLGDRPYRCPECGKTFNQRSHFLTHQRTHTGEKPFHCSKCNKSFRQKAHLLCHQNT
HLIY

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Reference Sequence Annotations:

Start on reference sequence 122

End on reference sequence 1573

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

ZNF774

Symbol Nomenclature :

ZNF774

Full Nomenclature :

zinc finger protein 774

GENEID :

342132

HGNC :

33108

Target GenBank:

BC067279.1

Reference Sequence Alignment

  

HsCD00288694        ATGTGGCTGGGGACTTCAGGGAAGAGTGGGTTACCTGGACACTGCTTAGAGAATCCTCTC
NM_001004309.2      ATGTGGCTGGGGACTTCAGGGAAGAGTGGGTTACCTGGACACTGCTTAGAGAATCCTCTC
                    ************************************************************

HsCD00288694        CAGGAATGCCACCCAGCACAGTTAGAAGAATGGGCTCTCAAAGGAATTTCCAGGCCTAGT
NM_001004309.2      CAGGAATGCCACCCAGCACAGTTAGAAGAATGGGCTCTCAAAGGAATTTCCAGGCCTAGT
                    ************************************************************

HsCD00288694        GTAATCTCCCAGCCGGAGCAGAAAGAAGAGCCATGGGTCCTACCACTCCAAAACTTTGAG
NM_001004309.2      GTAATCTCCCAGCCGGAGCAGAAAGAAGAGCCATGGGTCCTACCACTCCAAAACTTTGAG
                    ************************************************************

HsCD00288694        GCGAGGAAGATCCCGAGGGAAAGCCACACAGACTGTGAGCATCAGGTGGCAAAGCTCAAT
NM_001004309.2      GCGAGGAAGATCCCGAGGGAAAGCCACACAGACTGTGAGCATCAGGTGGCAAAGCTCAAT
                    ************************************************************

HsCD00288694        CAGGACAGTTCTGAAACAGCAGAACAATGTGGAACATCCTCAGAAAGGACCAATAAAGAT
NM_001004309.2      CAGGACAATTCTGAAACAGCAGAACAATGTGGAACATCCTCAGAAAGGACCAATAAAGAT
                    *******.****************************************************

HsCD00288694        CTTTCTCATACTCTTAGTTGGGGAGGAAACTGGGAGCAAGGCCTAGAATTAGAAGGGCAA
NM_001004309.2      CTTTCTCATACTCTTAGTTGGGGAGGAAACTGGGAGCAAGGCCTAGAATTAGAAGGGCAA
                    ************************************************************

HsCD00288694        CATGGAACCCTTCCAGGAGAGGGCCAGCTGGAGTCCTTTTCACAGGAGAGGGATTTAAAC
NM_001004309.2      CATGGAACCCTTCCAGGAGAGGGCCAGCTGGAGTCCTTTTCACAGGAGAGGGATTTAAAC
                    ************************************************************

HsCD00288694        AAGCTCCTGGATGGATATGTAGGAGAGAAGCCTATGTGTGCAGAATGCGGGAAAAGCTTT
NM_001004309.2      AAGCTCCTGGATGGATATGTAGGAGAGAAGCCTATGTGTGCAGAATGCGGGAAAAGCTTT
                    ************************************************************

HsCD00288694        AACCAGAGTTCCTATCTCATAAGACACCTAAGAACCCACACTGGCGAGAGGCCCTATACG
NM_001004309.2      AACCAGAGTTCCTATCTCATAAGACACCTAAGAACCCACACTGGCGAGAGGCCCTATACG
                    ************************************************************

HsCD00288694        TGCATTGAGTGTGGGAAAGGCTTCAAACAGAGCTCAGACCTTGTCACCCATCGCAGAACA
NM_001004309.2      TGCATTGAGTGTGGGAAAGGCTTCAAACAGAGCTCAGACCTTGTCACCCATCGCAGAACA
                    ************************************************************

HsCD00288694        CACACAGGAGAGAAGCCCTACCAATGCAAGGGGTGTGAGAAGAAATTCAGCGACAGCTCA
NM_001004309.2      CACACAGGAGAGAAGCCCTACCAATGCAAGGGGTGTGAGAAGAAATTCAGCGACAGCTCA
                    ************************************************************

HsCD00288694        ACACTCATCAAACATCAGAGAACCCACACAGGGGAGAGACCCTATGAGTGCCCAGAGTGT
NM_001004309.2      ACACTCATCAAACATCAGAGAACCCACACAGGGGAGAGACCCTATGAGTGCCCAGAGTGT
                    ************************************************************

HsCD00288694        GGAAAGACTTTTGGGCGGAAGCCACACCTCATAATGCACCAAAGAACCCACACAGGCGAG
NM_001004309.2      GGAAAGACTTTTGGGCGGAAGCCACACCTCATAATGCACCAAAGAACCCACACAGGCGAG
                    ************************************************************

HsCD00288694        AAGCCCTACGCGTGCCTGGAATGTCACAAAAGCTTCAGTCGAAGCTCAAATTTCATCACT
NM_001004309.2      AAGCCCTACGCGTGCCTGGAATGTCACAAAAGCTTCAGTCGAAGCTCAAATTTCATCACT
                    ************************************************************

HsCD00288694        CACCAGAGGACCCACACAGGGGTGAAGCCTTACAGGTGTAATGACTGTGGGGAGAGTTTT
NM_001004309.2      CACCAGAGGACCCACACAGGGGTGAAGCCTTACAGGTGTAATGACTGTGGGGAGAGTTTT
                    ************************************************************

HsCD00288694        AGCCAGAGCTCGGATTTGATTAAGCACCAACGAACCCACACGGGAGAACGGCCCTTCAAA
NM_001004309.2      AGCCAGAGCTCGGATTTGATTAAGCACCAACGAACCCACACGGGAGAACGGCCCTTCAAA
                    ************************************************************

HsCD00288694        TGCCCGGAGTGCGGGAAGGGCTTCAGAGATAGTTCTCATTTTGTAGCTCACATGAGCACT
NM_001004309.2      TGCCCGGAGTGCGGGAAGGGCTTCAGAGATAGTTCTCATTTTGTAGCTCACATGAGCACT
                    ************************************************************

HsCD00288694        CATTCAGGAGAGAGGCCTTTCAGTTGTCCTGACTGCCACAAAAGCTTCAGTCAGAGCTCA
NM_001004309.2      CATTCAGGAGAGAGGCCTTTCAGTTGTCCTGACTGCCACAAAAGCTTCAGTCAGAGCTCA
                    ************************************************************

HsCD00288694        CATTTGGTCACGCACCAAAGAACACACACAGGTGAGAGACCTTTTAAGTGCGAAAACTGT
NM_001004309.2      CATTTGGTCACGCACCAAAGAACACACACAGGTGAGAGACCTTTTAAGTGCGAAAACTGT
                    ************************************************************

HsCD00288694        GGGAAAGGATTCGCCGACAGCTCCGCCCTCATTAAGCACCAACGAATCCACACCGGAGAA
NM_001004309.2      GGGAAAGGATTCGCCGACAGCTCCGCCCTCATTAAGCACCAACGAATCCACACCGGAGAA
                    ************************************************************

HsCD00288694        AGACCCTACAAATGTGGAGAGTGTGGGAAGAGCTTCAATCAGAGCTCCCACTTTATTACC
NM_001004309.2      AGACCCTACAAATGTGGAGAGTGTGGGAAGAGCTTCAATCAGAGCTCCCACTTTATTACC
                    ************************************************************

HsCD00288694        CATCAGCGAATCCACTTAGGAGACAGGCCCTATCGATGTCCTGAGTGTGGCAAGACCTTC
NM_001004309.2      CATCAGCGAATCCACTTAGGAGACAGGCCCTATCGATGTCCTGAGTGTGGCAAGACCTTC
                    ************************************************************

HsCD00288694        AATCAGCGTTCCCATTTCCTCACACACCAGAGAACGCATACAGGAGAAAAACCTTTCCAC
NM_001004309.2      AATCAGCGTTCCCATTTCCTCACACACCAGAGAACGCATACAGGAGAAAAACCTTTCCAC
                    ************************************************************

HsCD00288694        TGTAGTAAATGTAACAAGAGCTTCCGTCAGAAAGCGCATCTTTTATGCCATCAAAACACC
NM_001004309.2      TGTAGTAAATGTAACAAGAGCTTCCGTCAGAAAGCGCATCTTTTATGCCATCAAAACACC
                    ************************************************************

HsCD00288694        CATTTGATTTAC
NM_001004309.2      CATTTGATTTAG
                    ***********