DNASU Plasmid | WDR5 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: WDR5

Gene Name: WD repeat domain 5

Original Clone ID:

2696

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

None

Comments:

None

Mutation / Discrepancy:

No / Yes

Publications:

None

Authors:

The ORFeome Collaboration

HsCD00288765

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1005nts

Open reading frame: 1 to 1005

Reference Sequence Alignment

ATGGCGACGGAGAAGAAGAAGCCCGAGACCGAGGCCGCCAGAGCACAGCCAACCCCTTCG
TCATCCGCCACTCAGAGCAAGCCTACACCTGTGAAGCCAAACTATGCTCTAAAGTTCACC
CTTGCTGGCCACACCAAAGCAGTGTCCTCCGTGAAATTCAGCCCGAATGGAGAGTGGCTG
GCAAGTTCATCTGCTGATAAACTTATTAAAATTTGGGGCGCGTATGATGGGAAATTTGAG
AAAACCATATCTGGTCACAAGCTGGGAATATCCGATGTAGCCTGGTCGTCAGATTCTAAC
CTTCTTGTTTCTGCCTCAGATGACAAAACCTTGAAGATATGGGACGTGAGCTCGGGCAAG
TGTCTGAAAACCCTGAAGGGACACAGTAATTATGTCTTTTGCTGCAACTTCAATCCCCAG
TCCAACCTTATTGTCTCAGGATCCTTTGACGAAAGCGTGAGGATATGGGATGTGAAAACA
GGGAAGTGCCTCAAGACTTTGCCAGCTCACTCGGATCCAGTCTCGGCCGTTCATTTTAAT
CGTGATGGATCCTTGATAGTTTCAAGTAGCTATGATGGTCTCTGTCGCATCTGGGACACC
GCCTCGGGCCAGTGCCTGAAGACGCTCATCGATGACGACAACCCCCCCGTGTCTTTTGTG
AAGTTCTCCCCGAACGGCAAATACATCCTGGCCGCCACGCTGGACAACACTCTGAAGCTC
TGGGACTACAGCAAGGGGAAGTGCCTGAAGACGTACACTGGCCACAAGAATGAGAAATAC
TGCATATTTGCCAATTTCTCTGTTACTGGTGGGAAGTGGATTGTGTCTGGCTCAGAGGAT
AACCTTGTTTACATCTGGAACCTTCAGACGAAAGAGATTGTACAGAAACTACAAGGCCAC
ACAGATGTCGTGATCTCAACAGCTTGTCACCCAACAGAAAACATCATCGCCTCTGCTGCG
CTAGAAAATGACAAAACAATTAAACTGTGGAAGAGTGACTGCTAC

Protein sequence predicted based on nucleotide sequence

MATEKKKPETEAARAQPTPSSSATQSKPTPVKPNYALKFTLAGHTKAVSSVKFSPNGEWL
ASSSADKLIKIWGAYDGKFEKTISGHKLGISDVAWSSDSNLLVSASDDKTLKIWDVSSGK
CLKTLKGHSNYVFCCNFNPQSNLIVSGSFDESVRIWDVKTGKCLKTLPAHSDPVSAVHFN
RDGSLIVSSSYDGLCRIWDTASGQCLKTLIDDDNPPVSFVKFSPNGKYILAATLDNTLKL
WDYSKGKCLKTYTGHKNEKYCIFANFSVTGGKWIVSGSEDNLVYIWNLQTKEIVQKLQGH
TDVVISTACHPTENIIASAALENDKTIKLWKSDCY

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Reference Sequence Annotations:

End on reference sequence 1150

Start on reference sequence 146

Mutations:

None

Discrepancy:

g13a,E5K

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

WDR5

Symbol Nomenclature :

WDR5

SYNONYM :

BIG-3; SWD3

Designation :

BMP2-induced 3-kb gene protein|SWD3, Set1c WD40 repeat protein, homolog|WD repeat-containing protein 5|WD-repeat protein 5

Full Nomenclature :

WD repeat domain 5

GENEID :

11091

HGNC :

12757

MIM :

609012

Vega :

OTTHUMG00000131707

Target GenBank:

BC001635.1

Reference Sequence Alignment

  

HsCD00288765        ATGGCGACGGAGAAGAAGAAGCCCGAGACCGAGGCCGCCAGAGCACAGCCAACCCCTTCG
NM_017588.2         ATGGCGACGGAGGAGAAGAAGCCCGAGACCGAGGCCGCCAGAGCACAGCCAACCCCTTCG
XM_005272163.1      ATGGCGACGGAGGAGAAGAAGCCCGAGACCGAGGCCGCCAGAGCACAGCCAACCCCTTCG
NM_052821.3         ATGGCGACGGAGGAGAAGAAGCCCGAGACCGAGGCCGCCAGAGCACAGCCAACCCCTTCG
                    ************.***********************************************

HsCD00288765        TCATCCGCCACTCAGAGCAAGCCTACACCTGTGAAGCCAAACTATGCTCTAAAGTTCACC
NM_017588.2         TCATCCGCCACTCAGAGCAAGCCTACACCTGTGAAGCCAAACTATGCTCTAAAGTTCACC
XM_005272163.1      TCATCCGCCACTCAGAGCAAGCCTACACCTGTGAAGCCAAACTATGCTCTAAAGTTCACC
NM_052821.3         TCATCCGCCACTCAGAGCAAGCCTACACCTGTGAAGCCAAACTATGCTCTAAAGTTCACC
                    ************************************************************

HsCD00288765        CTTGCTGGCCACACCAAAGCAGTGTCCTCCGTGAAATTCAGCCCGAATGGAGAGTGGCTG
NM_017588.2         CTTGCTGGCCACACCAAAGCAGTGTCCTCCGTGAAATTCAGCCCGAATGGAGAGTGGCTG
XM_005272163.1      CTTGCTGGCCACACCAAAGCAGTGTCCTCCGTGAAATTCAGCCCGAATGGAGAGTGGCTG
NM_052821.3         CTTGCTGGCCACACCAAAGCAGTGTCCTCCGTGAAATTCAGCCCGAATGGAGAGTGGCTG
                    ************************************************************

HsCD00288765        GCAAGTTCATCTGCTGATAAACTTATTAAAATTTGGGGCGCGTATGATGGGAAATTTGAG
NM_017588.2         GCAAGTTCATCTGCTGATAAACTTATTAAAATTTGGGGCGCGTATGATGGGAAATTTGAG
XM_005272163.1      GCAAGTTCATCTGCTGATAAACTTATTAAAATTTGGGGCGCGTATGATGGGAAATTTGAG
NM_052821.3         GCAAGTTCATCTGCTGATAAACTTATTAAAATTTGGGGCGCGTATGATGGGAAATTTGAG
                    ************************************************************

HsCD00288765        AAAACCATATCTGGTCACAAGCTGGGAATATCCGATGTAGCCTGGTCGTCAGATTCTAAC
NM_017588.2         AAAACCATATCTGGTCACAAGCTGGGAATATCCGATGTAGCCTGGTCGTCAGATTCTAAC
XM_005272163.1      AAAACCATATCTGGTCACAAGCTGGGAATATCCGATGTAGCCTGGTCGTCAGATTCTAAC
NM_052821.3         AAAACCATATCTGGTCACAAGCTGGGAATATCCGATGTAGCCTGGTCGTCAGATTCTAAC
                    ************************************************************

HsCD00288765        CTTCTTGTTTCTGCCTCAGATGACAAAACCTTGAAGATATGGGACGTGAGCTCGGGCAAG
NM_017588.2         CTTCTTGTTTCTGCCTCAGATGACAAAACCTTGAAGATATGGGACGTGAGCTCGGGCAAG
XM_005272163.1      CTTCTTGTTTCTGCCTCAGATGACAAAACCTTGAAGATATGGGACGTGAGCTCGGGCAAG
NM_052821.3         CTTCTTGTTTCTGCCTCAGATGACAAAACCTTGAAGATATGGGACGTGAGCTCGGGCAAG
                    ************************************************************

HsCD00288765        TGTCTGAAAACCCTGAAGGGACACAGTAATTATGTCTTTTGCTGCAACTTCAATCCCCAG
NM_017588.2         TGTCTGAAAACCCTGAAGGGACACAGTAATTATGTCTTTTGCTGCAACTTCAATCCCCAG
XM_005272163.1      TGTCTGAAAACCCTGAAGGGACACAGTAATTATGTCTTTTGCTGCAACTTCAATCCCCAG
NM_052821.3         TGTCTGAAAACCCTGAAGGGACACAGTAATTATGTCTTTTGCTGCAACTTCAATCCCCAG
                    ************************************************************

HsCD00288765        TCCAACCTTATTGTCTCAGGATCCTTTGACGAAAGCGTGAGGATATGGGATGTGAAAACA
NM_017588.2         TCCAACCTTATTGTCTCAGGATCCTTTGACGAAAGCGTGAGGATATGGGATGTGAAAACA
XM_005272163.1      TCCAACCTTATTGTCTCAGGATCCTTTGACGAAAGCGTGAGGATATGGGATGTGAAAACA
NM_052821.3         TCCAACCTTATTGTCTCAGGATCCTTTGACGAAAGCGTGAGGATATGGGATGTGAAAACA
                    ************************************************************

HsCD00288765        GGGAAGTGCCTCAAGACTTTGCCAGCTCACTCGGATCCAGTCTCGGCCGTTCATTTTAAT
NM_017588.2         GGGAAGTGCCTCAAGACTTTGCCAGCTCACTCGGATCCAGTCTCGGCCGTTCATTTTAAT
XM_005272163.1      GGGAAGTGCCTCAAGACTTTGCCAGCTCACTCGGATCCAGTCTCGGCCGTTCATTTTAAT
NM_052821.3         GGGAAGTGCCTCAAGACTTTGCCAGCTCACTCGGATCCAGTCTCGGCCGTTCATTTTAAT
                    ************************************************************

HsCD00288765        CGTGATGGATCCTTGATAGTTTCAAGTAGCTATGATGGTCTCTGTCGCATCTGGGACACC
NM_017588.2         CGTGATGGATCCTTGATAGTTTCAAGTAGCTATGATGGTCTCTGTCGCATCTGGGACACC
XM_005272163.1      CGTGATGGATCCTTGATAGTTTCAAGTAGCTATGATGGTCTCTGTCGCATCTGGGACACC
NM_052821.3         CGTGATGGATCCTTGATAGTTTCAAGTAGCTATGATGGTCTCTGTCGCATCTGGGACACC
                    ************************************************************

HsCD00288765        GCCTCGGGCCAGTGCCTGAAGACGCTCATCGATGACGACAACCCCCCCGTGTCTTTTGTG
NM_017588.2         GCCTCAGGCCAGTGCCTGAAGACGCTCATCGATGACGACAACCCCCCCGTGTCTTTTGTG
XM_005272163.1      GCCTCAGGCCAGTGCCTGAAGACGCTCATCGATGACGACAACCCCCCCGTGTCTTTTGTG
NM_052821.3         GCCTCAGGCCAGTGCCTGAAGACGCTCATCGATGACGACAACCCCCCCGTGTCTTTTGTG
                    *****.******************************************************

HsCD00288765        AAGTTCTCCCCGAACGGCAAATACATCCTGGCCGCCACGCTGGACAACACTCTGAAGCTC
NM_017588.2         AAGTTCTCCCCGAACGGCAAATACATCCTGGCCGCCACGCTGGACAACACTCTGAAGCTC
XM_005272163.1      AAGTTCTCCCCGAACGGCAAATACATCCTGGCCGCCACGCTGGACAACACTCTGAAGCTC
NM_052821.3         AAGTTCTCCCCGAACGGCAAATACATCCTGGCCGCCACGCTGGACAACACTCTGAAGCTC
                    ************************************************************

HsCD00288765        TGGGACTACAGCAAGGGGAAGTGCCTGAAGACGTACACTGGCCACAAGAATGAGAAATAC
NM_017588.2         TGGGACTACAGCAAGGGGAAGTGCCTGAAGACGTACACTGGCCACAAGAATGAGAAATAC
XM_005272163.1      TGGGACTACAGCAAGGGGAAGTGCCTGAAGACGTACACTGGCCACAAGAATGAGAAATAC
NM_052821.3         TGGGACTACAGCAAGGGGAAGTGCCTGAAGACGTACACTGGCCACAAGAATGAGAAATAC
                    ************************************************************

HsCD00288765        TGCATATTTGCCAATTTCTCTGTTACTGGTGGGAAGTGGATTGTGTCTGGCTCAGAGGAT
NM_017588.2         TGCATATTTGCCAATTTCTCTGTTACTGGTGGGAAGTGGATTGTGTCTGGCTCAGAGGAT
XM_005272163.1      TGCATATTTGCCAATTTCTCTGTTACTGGTGGGAAGTGGATTGTGTCTGGCTCAGAGGAT
NM_052821.3         TGCATATTTGCCAATTTCTCTGTTACTGGTGGGAAGTGGATTGTGTCTGGCTCAGAGGAT
                    ************************************************************

HsCD00288765        AACCTTGTTTACATCTGGAACCTTCAGACGAAAGAGATTGTACAGAAACTACAAGGCCAC
NM_017588.2         AACCTTGTTTACATCTGGAACCTTCAGACGAAAGAGATTGTACAGAAACTACAAGGCCAC
XM_005272163.1      AACCTTGTTTACATCTGGAACCTTCAGACGAAAGAGATTGTACAGAAACTACAAGGCCAC
NM_052821.3         AACCTTGTTTACATCTGGAACCTTCAGACGAAAGAGATTGTACAGAAACTACAAGGCCAC
                    ************************************************************

HsCD00288765        ACAGATGTCGTGATCTCAACAGCTTGTCACCCAACAGAAAACATCATCGCCTCTGCTGCG
NM_017588.2         ACAGATGTCGTGATCTCAACAGCTTGTCACCCAACAGAAAACATCATCGCCTCTGCTGCG
XM_005272163.1      ACAGATGTCGTGATCTCAACAGCTTGTCACCCAACAGAAAACATCATCGCCTCTGCTGCG
NM_052821.3         ACAGATGTCGTGATCTCAACAGCTTGTCACCCAACAGAAAACATCATCGCCTCTGCTGCG
                    ************************************************************

HsCD00288765        CTAGAAAATGACAAAACAATTAAACTGTGGAAGAGTGACTGCTAC
NM_017588.2         CTAGAAAATGACAAAACAATTAAACTGTGGAAGAGTGACTGCTAA
XM_005272163.1      CTAGAAAATGACAAAACAATTAAACTGTGGAAGAGTGACTGCTAA
NM_052821.3         CTAGAAAATGACAAAACAATTAAACTGTGGAAGAGTGACTGCTAA
                    ********************************************.