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FABP6 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  FABP6
Gene Name:  fatty acid binding protein 6, ileal
Sequence             
Original Clone ID: 22778
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
HsCD00288921

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 387nts         Open reading frame : 1 to 387
Reference Sequence Alignment

ATGGCTTTCACCGGCAAGTTCGAGATGGAGAGTGAGAAGAATTATGATGAGTTCATGAAG
CTCCTTGGGATCTCCAGCGATGTAATCGAAAAGGCCCACAACTTCAAGATCGTCACGGAG
GTGCAGCAGGATGGGCAGGACTTCACTTGGTCCCAGCACTACTACGGGGGCCACACCATG
ACCAACAAGTTCACTGTTGGCAAGGAAAGCAACATACAGACAATGGGGGGCAAGACGTTC
AAGGCCACTGTGCAGATGGAGGGCGGGAAGCTGGTGGTGAATTTCCCCAACTATCACCAG
ACCTCAGAGATCGTGGGTGACAAGCTGGTGGAGGTCTCCACCATCGGAGGCGTGACCTAT
GAGCGCGTGAGCAAGAGACTGGCCTAC


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 485
Start on reference sequence 99
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
GTACAAAAAAGTTGGC

3' Linker Sequence:
5p (goi-stop)CCAACTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223

pENTR223 in advanced viewed

Synonyms: pDONR223, pENTR223-XnCDSYn
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp): 2815
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Mark Vidal
Publications: PMID: 15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 2220 2753
gene fragment pas 5p stem of pBR primose assembly site 63 73
gene fragment pas prime 3p stem of pBR pas(L) 105 116
recombination site attL1 attL1 recombination site 570 665
recombination site attL2 attL2 recombination site 793 696
selectable marker SpnR spectinomycin resistance gene 1062 2072
trxn termination sequence T1-T2 rrnBT1 and T2 terminators 510 224

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : FABP6
Symbol Nomenclature : FABP6
SYNONYM : I-15P; I-BABP; I-BALB; I-BAP; ILBP; ILBP3; ILLBP
Designation : GT|gastrotropin|ileal bile acid binding protein|ileal lipid-binding protein|illeal lipid-binding protein|intestinal 15 kDa protein
Full Nomenclature : fatty acid binding protein 6, ileal
GENEID : 2172
HGNC : 3561
MIM : 600422
Vega : OTTHUMG00000130329
Target GenBank: BC022489.1

 

Reference Sequence Alignment

  

HsCD00288921        ------------------------------------------------------------
NM_001040442.1      ATGAAGACAGTGACGATGATGATGGTGGTGGAGATGCAGGCGCTGACTCAGGTTCTGAGA
NM_001130958.1      ATGAAGACAGTGACGATGATGATGGTGGTGGAGATGCAGGCGCTGACTCAGGTTCTGAGA
NM_001445.2         ------------------------------------------------------------
                                                                                

HsCD00288921        ------------------------------------------------------------
NM_001040442.1      GCTGTGTTGTCTGCGTGCACATGGGTGAGCCGGAAAGGAGACCTGCAGAGAATGAAACAG
NM_001130958.1      GCTGTGTTGTCTGCGTGCACATGGGTGAGCCGGAAAGGAGACCTGCAGAGAATGAAACAG
NM_001445.2         ------------------------------------------------------------
                                                                                

HsCD00288921        ---------------------------ATGGCTTTCACCGGCAAGTTCGAGATGGAGAGT
NM_001040442.1      ACACATAAAGGAAAGCCTCCCAGCAGCATGGCTTTCACCGGCAAGTTCGAGATGGAGAGT
NM_001130958.1      ACACATAAAGGAAAGCCTCCCAGCAGCATGGCTTTCACCGGCAAGTTCGAGATGGAGAGT
NM_001445.2         ---------------------------ATGGCTTTCACCGGCAAGTTCGAGATGGAGAGT
                                               *********************************

HsCD00288921        GAGAAGAATTATGATGAGTTCATGAAGCTCCTTGGGATCTCCAGCGATGTAATCGAAAAG
NM_001040442.1      GAGAAGAATTATGATGAGTTCATGAAGCTCCTTGGGATCTCCAGCGATGTAATCGAAAAG
NM_001130958.1      GAGAAGAATTATGATGAGTTCATGAAGCTCCTTGGGATCTCCAGCGATGTAATCGAAAAG
NM_001445.2         GAGAAGAATTATGATGAGTTCATGAAGCTCCTTGGGATCTCCAGCGATGTAATCGAAAAG
                    ************************************************************

HsCD00288921        GCCCACAACTTCAAGATCGTCACGGAGGTGCAGCAGGATGGGCAGGACTTCACTTGGTCC
NM_001040442.1      GCCCGCAACTTCAAGATCGTCACGGAGGTGCAGCAGGATGGGCAGGACTTCACTTGGTCC
NM_001130958.1      GCCCGCAACTTCAAGATCGTCACGGAGGTGCAGCAGGATGGGCAGGACTTCACTTGGTCC
NM_001445.2         GCCCGCAACTTCAAGATCGTCACGGAGGTGCAGCAGGATGGGCAGGACTTCACTTGGTCC
                    ****.*******************************************************

HsCD00288921        CAGCACTACTACGGGGGCCACACCATGACCAACAAGTTCACTGTTGGCAAGGAAAGCAAC
NM_001040442.1      CAGCACTACTCCGGGGGCCACACCATGACCAACAAGTTCACTGTTGGCAAGGAAAGCAAC
NM_001130958.1      CAGCACTACTCCGGGGGCCACACCATGACCAACAAGTTCACTGTTGGCAAGGAAAGCAAC
NM_001445.2         CAGCACTACTCCGGGGGCCACACCATGACCAACAAGTTCACTGTTGGCAAGGAAAGCAAC
                    **********.*************************************************

HsCD00288921        ATACAGACAATGGGGGGCAAGACGTTCAAGGCCACTGTGCAGATGGAGGGCGGGAAGCTG
NM_001040442.1      ATACAGACAATGGGGGGCAAGACGTTCAAGGCCACTGTGCAGATGGAGGGCGGGAAGCTG
NM_001130958.1      ATACAGACAATGGGGGGCAAGACGTTCAAGGCCACTGTGCAGATGGAGGGCGGGAAGCTG
NM_001445.2         ATACAGACAATGGGGGGCAAGACGTTCAAGGCCACTGTGCAGATGGAGGGCGGGAAGCTG
                    ************************************************************

HsCD00288921        GTGGTGAATTTCCCCAACTATCACCAGACCTCAGAGATCGTGGGTGACAAGCTGGTGGAG
NM_001040442.1      GTGGTGAATTTCCCCAACTATCACCAGACCTCAGAGATCGTGGGTGACAAGCTGGTGGAG
NM_001130958.1      GTGGTGAATTTCCCCAACTATCACCAGACCTCAGAGATCGTGGGTGACAAGCTGGTGGAG
NM_001445.2         GTGGTGAATTTCCCCAACTATCACCAGACCTCAGAGATCGTGGGTGACAAGCTGGTGGAG
                    ************************************************************

HsCD00288921        GTCTCCACCATCGGAGGCGTGACCTATGAGCGCGTGAGCAAGAGACTGGCCTAC
NM_001040442.1      GTCTCCACCATCGGAGGCGTGACCTATGAGCGCGTGAGCAAGAGACTGGCCTAA
NM_001130958.1      GTCTCCACCATCGGAGGCGTGACCTATGAGCGCGTGAGCAAGAGACTGGCCTAA
NM_001445.2         GTCTCCACCATCGGAGGCGTGACCTATGAGCGCGTGAGCAAGAGACTGGCCTAA
                    *****************************************************.

Pathways

Reactome : Bile acid and bile salt metabolism
Reactome : Metabolism
Reactome : Metabolism of lipids and lipoproteins
Reactome : Recycling of bile acids and salts

Protein

UniProt : P51161
HPRD : 02689

Clone

Cloning Information : 472158
HIP Master Clone ID : 395076
Original Clone ID : 22778

Organism


TAX_ID : 9606
Species Specific ID: 2172

Genome

Chromosome : 5
Map Location : 5q33.3-q34
Ensembl : ENSG00000170231

Reagents

Labome : FABP6-antibody