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UBE2S (Homo sapiens) in pENTR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  UBE2S
Gene Name:  ubiquitin-conjugating enzyme E2S
Sequence             
Original Clone ID: 35459
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
HsCD00289065

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 669nts         Open reading frame : 1 to 669
Reference Sequence Alignment

ATGAACTCCAACGTGGAGAACCTGCCCCCGCACATCATCCGCCTGGTGTACAAGGAGGTG
ACGACACTGACCGCAGACCCACCCGATGGCATCAAGGTCTTTCCCAACGAGGAGGACCTC
ACCGACCTCCAGGTCACCATCGAGGGCCCTGAAGGGACCCCATATGCTGGAGGTCTGTTC
CGCATGAAACTCCTGCTGGGGAAGGACTTCCCTGCCTCCCCACCCAAGGGCTACTTCCTG
ACCAAGATCTTCCACCTGAACGTGGGCGCCAATGGCGAGATCTGCGTCAACGTGCTCAAG
AGGGACTGGACGGCTGAGCTGGGCATCCGACACGTACTGCTGACCATCAGGTGCCTGCTG
ATCCACCCTAACCCCGAGTCTGCACTCAACGAGGAGGCGGGCCGCCTGCTCTTGGAGAAC
TCCGAGGAGTATGCGGCCCGGGCCCGTCTGCTCACAGAGATCCACGGGGGCGCCGGCGGG
CCCAGCGGCAGGGCCGAAGCCGGGCGGGCCCTGGCCAGTGGCACTGCAGCTTCCTCCACC
GACCCTGGGGCCCCAGGGGGCCTGGGAGGGGCTGAGGGTCCCATGGCCAAGAAGCATGCT
GGCGAGCGCGATAAGAAGCTGGCGGCCAAGAAAAAGACGGACAAGAAGCGGGCGCTGCGG
CGGCTGTAC


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 693
Start on reference sequence 25
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
GTACAAAAAAGTTGGC

3' Linker Sequence:
5p (goi-stop)CCAACTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223

pENTR223 in advanced viewed

Synonyms: pDONR223, pENTR223-XnCDSYn
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp): 2815
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Mark Vidal
Publications: PMID: 15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 2220 2753
gene fragment pas 5p stem of pBR primose assembly site 63 73
gene fragment pas prime 3p stem of pBR pas(L) 105 116
recombination site attL1 attL1 recombination site 570 665
recombination site attL2 attL2 recombination site 793 696
selectable marker SpnR spectinomycin resistance gene 1062 2072
trxn termination sequence T1-T2 rrnBT1 and T2 terminators 510 224

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : UBE2S
Symbol Nomenclature : UBE2S
SYNONYM : E2-EPF; E2EPF; EPF5
Designation : E2-EPF5|ubiquitin carrier protein S|ubiquitin-conjugating enzyme E2 S|ubiquitin-conjugating enzyme E2-24 kD|ubiquitin-conjugating enzyme E2-24 kDa|ubiquitin-conjugating enzyme E2-EPF5|ubiquitin-protein ligase S
Full Nomenclature : ubiquitin-conjugating enzyme E2S
GENEID : 27338
Locus Tag : OK/SW-cl.73
HGNC : 17895
MIM : 610309
Vega : OTTHUMG00000180811
Target GenBank: BC066948.1

 

Reference Sequence Alignment

  

HsCD00289065      ATGAACTCCAACGTGGAGAACCTGCCCCCGCACATCATCCGCCTGGTGTACAAGGAGGTG
NM_014501.2       ATGAACTCCAACGTGGAGAACCTACCCCCGCACATCATCCGCCTGGTGTACAAGGAGGTG
                  ***********************.************************************

HsCD00289065      ACGACACTGACCGCAGACCCACCCGATGGCATCAAGGTCTTTCCCAACGAGGAGGACCTC
NM_014501.2       ACGACACTGACCGCAGACCCACCCGATGGCATCAAGGTCTTTCCCAACGAGGAGGACCTC
                  ************************************************************

HsCD00289065      ACCGACCTCCAGGTCACCATCGAGGGCCCTGAAGGGACCCCATATGCTGGAGGTCTGTTC
NM_014501.2       ACCGACCTCCAGGTCACCATCGAGGGCCCTGAGGGGACCCCATATGCTGGAGGTCTGTTC
                  ********************************.***************************

HsCD00289065      CGCATGAAACTCCTGCTGGGGAAGGACTTCCCTGCCTCCCCACCCAAGGGCTACTTCCTG
NM_014501.2       CGCATGAAACTCCTGCTGGGGAAGGACTTCCCTGCCTCCCCACCCAAGGGCTACTTCCTG
                  ************************************************************

HsCD00289065      ACCAAGATCTTCCACCTGAACGTGGGCGCCAATGGCGAGATCTGCGTCAACGTGCTCAAG
NM_014501.2       ACCAAGATCTTCCACCCGAACGTGGGCGCCAATGGCGAGATCTGCGTCAACGTGCTCAAG
                  **************** *******************************************

HsCD00289065      AGGGACTGGACGGCTGAGCTGGGCATCCGACACGTACTGCTGACCATCAGGTGCCTGCTG
NM_014501.2       AGGGACTGGACGGCTGAGCTGGGCATCCGACACGTACTGCTGACCATCAAGTGCCTGCTG
                  *************************************************.**********

HsCD00289065      ATCCACCCTAACCCCGAGTCTGCACTCAACGAGGAGGCGGGCCGCCTGCTCTTGGAGAAC
NM_014501.2       ATCCACCCTAACCCCGAGTCTGCACTCAACGAGGAGGCGGGCCGCCTGCTCTTGGAGAAC
                  ************************************************************

HsCD00289065      TCCGAGGAGTATGCGGCCCGGGCCCGTCTGCTCACAGAGATCCACGGGGGCGCCGGCGGG
NM_014501.2       TACGAGGAGTATGCGGCTCGGGCCCGTCTGCTCACAGAGATCCACGGGGGCGCCGGCGGG
                  *.*************** ******************************************

HsCD00289065      CCCAGCGGCAGGGCCGAAGCCGGGCGGGCCCTGGCCAGTGGCACTGCAGCTTCCTCCACC
NM_014501.2       CCCAGCGGCAGGGCCGAAGCCGGTCGGGCCCTGGCCAGTGGCACTGAAGCTTCCTCCACC
                  *********************** **********************.*************

HsCD00289065      GACCCTGGGGCCCCAGGGGGCCTGGGAGGGGCTGAGGGTCCCATGGCCAAGAAGCATGCT
NM_014501.2       GACCCTGGGGCCCCAGGGGGCCCGGGAGGGGCTGAGGGTCCCATGGCCAAGAAGCATGCT
                  ********************** *************************************

HsCD00289065      GGCGAGCGCGATAAGAAGCTGGCGGCCAAGAAAAAGACGGACAAGAAGCGGGCGCTGCGG
NM_014501.2       GGCGAGCGCGATAAGAAGCTGGCGGCCAAGAAAAAGACGGACAAGAAGCGGGCGCTGCGG
                  ************************************************************

HsCD00289065      CGGCTGTAC
NM_014501.2       CGGCTGTAG
                  ******** 

Pathways

Panther : Ubiquitin proteasome pathway
Reactome : Adaptive Immune System
Reactome : Antigen processing: Ubiquitination & Proteasome degradation
Reactome : Class I MHC mediated antigen processing & presentation
Reactome : Immune System
WikiPathway : Epithelium TarBase
WikiPathway : Lymphocyte TarBase

Clone

Cloning Information : 472302
HIP Master Clone ID : 395220
Original Clone ID : 35459

Organism


TAX_ID : 9606
Species Specific ID: 27338

Genome

Chromosome : 19
Map Location : 19q13.43
Ensembl : ENSG00000108106

Reagents

Labome : UBE2S-antibody