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DUSP1 (Homo sapiens) in pSGX4 (His and Smt3-tagged bacterial expression vector)

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Explanation of Terms


Gene: Gene Symbol:  DUSP1
Gene Name:  dual specificity phosphatase 1
Sequence             
Original Clone ID: 8651a3BSt4p1
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pSGX4               Format:  CLOSED
Source: New York SGX Research Center for Structural Genomics
Description: None
Comments: N-terminal 6-His and Smt3, cleavable, TOPO
Mutation/
Discrepancy :
No / No
Publications: None
Authors: PSI
New York SGX Research Center for Structural Genomics
HsCD00291599

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AVAILABLE
No restriction
Special MTA: PSI/Smt3/SUMO

 

Insert sequence: 435nts         Open reading frame : 51 to 485
Reference Sequence Alignment

AGGATAACGATATTATTGAGGCTCACAGAGAACAGATTGGTGGATCCCTTCAGGGTGGCC
CGGTGGAAATCCTGCCCTTTCTGTACCTGGGCAGTGCGTATCACGCTTCCCGCAAGGACA
TGCTGGATGCCTTGGGCATAACTGCCTTGATCAACGTCTCAGCCAATTGTCCCAACCATT
TTGAGGGTCACTACCAGTACAAGAGCATCCCTGTGGAGGACAACCACAAGGCAGACATCA
GCTCTTGGTTCAACGAGGCCATTGACTTCATAGACTCCATCAAGAATGCTGGAGGAAGGG
TGTTTGTCCACTGCCAGGCAGGCATTTCCCGGTCAGCCACCATCTGCCTTGCTTACCTTA
TGAGGACTAATCGAGTCAAGCTGGACGAGGCCTTTGAGTTTGTGAAGCAGAGGCGAAGCA
TCATCTCTCCCAACTTCAGCTTCATGGGCCAGCTGCTGCAGTTTGAGTCCCAGGTGCTGG
CTTAAGGGTAACTCGAGCACCACCACCACCACC


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 435
Start on reference sequence 1
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
ACAGAGAACAGATTGGTGGATCCCTT

3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: ProteinPurified: Tested_Not_Purified
SolubleProtein: Protein_Soluble
ProteinExpressed: Protein_Confirmed
Find experimental protocols in NYSGXRC-8651a
Recommended expression in: BL21(DE3)-Codon+RIL
       DyNA Vector Map

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Vector Name: pSGX4

pSGX4 in advanced viewed

Synonyms: pSGX-4, pSGX4_BS
Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Bacterial (E. coli) expression vector with T7 promotor, adds N-terminal 6xHis and smt-3 tags, cleavage site; kanamycin resistance; TOPO cloning.
Comments: Modified from pET26b from Novagen. N-terminal 6xHis-tag followed by smt3 fusion protein. Smt3 fusion cloned into NheI and BamHI sites. TOPO adaptation at BamHI and XhoI sites.
Size (bp): 5611
Parent Vector: None
Empty Vector: None
Properties: TOPO Cloning, bacterial expression, in vitro transcription, with tag/fusion/marker
Author Name: New York SGX Research Center for Structural Genomics
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
TOPO recognition site TOPO TOPO cloning site 5435 5444
bacterial origin ColE ColE1 bacterial origin of replication 1421 2103
gene fragment ROP ROP 2527 2703
promoter T7 T7 promoter 4984 5003
protease cleavage site ULP site ULP cleavage site (recognizes the tertiary structure of SUMO) 5140 5140
repressor protein gene LacI lacI cds 3515 4351
ribosome binding site RBS ribosome binding site (RBS) 5062 5068
selectable marker KanR kanamycin resistance gene 563 1375
tag Smt3 Smt3 tag 5140 5424
tag His 6xHis tag 5083 5100
trxn regulatory element lac operator lac operator 5003 5025
trxn termination sequence T7 term T7 termination sequence 5539 5585

 

  • Gene
  • Protein
  • Clone
  • Experimental
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : DUSP1
Symbol Nomenclature : DUSP1
SYNONYM : CL100; HVH1; MKP-1; MKP1; PTPN10
Designation : MAP kinase phosphatase 1|dual specificity protein phosphatase 1|dual specificity protein phosphatase hVH1|mitogen-activated protein kinase phosphatase 1|protein-tyrosine phosphatase CL100|serine/threonine specific protein phosphatase
Full Nomenclature : dual specificity phosphatase 1
GENEID : 1843
HGNC : 3064
MIM : 600714
Vega : OTTHUMG00000130523
Target GenBank: NP_004408

 

Reference Sequence Alignment

  

HsCD00291599      ------------------------------------------------------------
NM_004417.3       ATGGTCATGGAAGTGGGCACCCTGGACGCTGGAGGCCTGCGGGCGCTGCTGGGGGAGCGA
                                                                              

HsCD00291599      ------------------------------------------------------------
NM_004417.3       GCGGCGCAATGCCTGCTGCTGGACTGCCGCTCCTTCTTCGCTTTCAACGCCGGCCACATC
                                                                              

HsCD00291599      ------------------------------------------------------------
NM_004417.3       GCCGGCTCTGTCAACGTGCGCTTCAGCACCATCGTGCGGCGCCGGGCCAAGGGCGCCATG
                                                                              

HsCD00291599      ------------------------------------------------------------
NM_004417.3       GGCCTGGAGCACATCGTGCCCAACGCCGAGCTCCGCGGCCGCCTGCTGGCCGGCGCCTAC
                                                                              

HsCD00291599      ------------------------------------------------------------
NM_004417.3       CACGCCGTGGTGTTGCTGGACGAGCGCAGCGCCGCCCTGGACGGCGCCAAGCGCGACGGC
                                                                              

HsCD00291599      ------------------------------------------------------------
NM_004417.3       ACCCTGGCCCTGGCGGCCGGCGCGCTCTGCCGCGAGGCGCGCGCCGCGCAAGTCTTCTTC
                                                                              

HsCD00291599      ------------------------------------------------------------
NM_004417.3       CTCAAAGGAGGATACGAAGCGTTTTCGGCTTCCTGCCCGGAGCTGTGCAGCAAACAGTCG
                                                                              

HsCD00291599      ----------------------------------------AGGATAACGATATTATTGAG
NM_004417.3       ACCCCCATGGGGCTCAGCCTTCCCCTGAGTACTAGCGTCCCTGACAGCGCGGAATCTGGG
                                                          . ** *.**. .::: **.*

HsCD00291599      GCTCACAGAGAACAGATTGGTGGATCCCTTCAGGGTGGCCCGGTGGAAATCCTGCCCTTT
NM_004417.3       TGCAGTTCCTGCAGTACCCCACTCTACGATCAGGGTGGCCCGGTGGAAATCCTGCCCTTT
                     .. : . .... *    :  .*.* :*******************************

HsCD00291599      CTGTACCTGGGCAGTGCGTATCACGCTTCCCGCAAGGACATGCTGGATGCCTTGGGCATA
NM_004417.3       CTGTACCTGGGCAGTGCGTATCACGCTTCCCGCAAGGACATGCTGGATGCCTTGGGCATC
                  ***********************************************************.

HsCD00291599      ACTGCCTTGATCAACGTCTCAGCCAATTGTCCCAACCATTTTGAGGGTCACTACCAGTAC
NM_004417.3       ACTGCCTTGATCAACGTCTCAGCCAATTGTCCCAACCATTTTGAGGGTCACTACCAGTAC
                  ************************************************************

HsCD00291599      AAGAGCATCCCTGTGGAGGACAACCACAAGGCAGACATCAGCTCTTGGTTCAACGAGGCC
NM_004417.3       AAGAGCATCCCTGTGGAGGACAACCACAAGGCAGACATCAGCTCCTGGTTCAACGAGGCC
                  ******************************************** ***************

HsCD00291599      ATTGACTTCATAGACTCCATCAAGAATGCTGGAGGAAGGGTGTTTGTCCACTGCCAGGCA
NM_004417.3       ATTGACTTCATAGACTCCATCAAGAATGCTGGAGGAAGGGTGTTTGTCCACTGCCAGGCA
                  ************************************************************

HsCD00291599      GGCATTTCCCGGTCAGCCACCATCTGCCTTGCTTACCTTATGAGGACTAATCGAGTCAAG
NM_004417.3       GGCATTTCCCGGTCAGCCACCATCTGCCTTGCTTACCTTATGAGGACTAATCGAGTCAAG
                  ************************************************************

HsCD00291599      CTGGACGAGGCCTTTGAGTTTGTGAAGCAGAGGCGAAGCATCATCTCTCCCAACTTCAGC
NM_004417.3       CTGGACGAGGCCTTTGAGTTTGTGAAGCAGAGGCGAAGCATCATCTCTCCCAACTTCAGC
                  ************************************************************

HsCD00291599      TTCATGGGCCAGCTGCTGCAGTTTGAGTCCCAGGTGCTG---------------------
NM_004417.3       TTCATGGGCCAGCTGCTGCAGTTTGAGTCCCAGGTGCTGGCTCCGCACTGTTCGGCAGAG
                  ***************************************                     

HsCD00291599      -----------------GCTTAAGGGTAACTCGAGCACCACCACCACCACC---------
NM_004417.3       GCTGGGAGCCCCGCCATGGCTGTGCTCGACCGAGGCACCTCCACCACCACCGTGTTCAAC
                                   *  *.:*   .**  ..*****:***********         

HsCD00291599      ------------------------------------------------------------
NM_004417.3       TTCCCCGTCTCCATCCCTGTCCACTCCACGAACAGTGCGCTGAGCTACCTTCAGAGCCCC
                                                                              

HsCD00291599      ------------------------
NM_004417.3       ATTACGACCTCTCCCAGCTGCTGA
                                          

Protein

SMART domain : DSPc : Dual specificity phosphatase, catalytic domain
SMART domain : PTPc_motif : Protein tyrosine phosphatase, catalytic domain motif
Structural Annotation Wiki : TOPSAN
UniProt : B4DU40
UniProt : P28562
HPRD : 02835

Clone

Cloning Information : 474904
HIP Master Clone ID : 396410
Original Clone ID : 8651a3BSt4p1

Experimental

TargetTrack - Experimental data : NYSGXRC-8651a

Organism


TAX_ID : 9606
Species Specific ID: None

Genome

Chromosome : 5
Map Location : 5q34
Ensembl : ENSG00000120129

Reagents

Labome : MKP-1-antibody