DNASU Plasmid | CX3CR1 (Homo sapiens) in pJ2 (Cloning)

Explanation of Terms

Gene:

Gene Symbol: CX3CR1

Gene Name: chemokine (C-X3-C motif) receptor 1

Original Clone ID:

ATCG3D_10

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pJ2

Format:

CLOSED

Source:

Accelerated Technologies Center for Gene to 3D Structure

Description:

G-protein coupled receptor

Comments:

Clone insert was fully verified. The insert sequence displayed below was provided by clone submitter. Codon Usage Engineered in Gene Composer for Expression in E. coli and BV. Supplied in the Vendor's Vector.

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

PSI
Accelerated Technologies Center for Gene to 3D Structure

HsCD00292913

Price:

AVAILABLE

No restriction
Special MTA: PSI

Insert Sequence
Protein Sequence

Insert sequence: 1167nts

Open reading frame: 7 to 1173

Reference Sequence Alignment

GGATCCATGAAAACGATCATCGCCCTGAGCTATATCTTCTGCCTTGTTTTCGCAGACTAT
AAGGACGATGACGATATGGACCAGTTCCCTGAATCTGTGACGGAGAATTTCGAGTACGAC
GATCTGGCTGAGGCCTGTTATATTGGCGATATTGTGGTGTTCGGTACCGTTTTCCTCTCC
ATCTTTTATAGCGTGATCTTCGCCATCGGGCTGGTGGGAAACCTCCTCGTGGTGTTTGCC
CTGACTAACTCCAAGAAGCCAAAATCCGTTACCGACATCTACCTGCTGAATCTCGCTCTG
TCTGACCTGCTGTTCGTTGCTACACTGCCCTTCTGGACCCATTACCTGATCAATGAAAAA
GGCCTCCACAACGCCATGTGTAAATTTACCACAGCTTTCTTTTTCATCGGGTTCTTCGGT
TCCATCTTCTTCATTACCGTCATTAGTATCGACCGTTACCTGGCCATTGTGCTGGCCGCT
AACTCCATGAATAACCGCACTGTTCAGCACGGTGTGACAATCTCCCTGGGCGTCTGGGCC
GCAGCTATTCTGGTGGCCGCCCCTCAGTTCATGTTCACAAAACAAAAAGAGAACGAGTGT
CTCGGCGACTACCCTGAGGTGCTCCAGGAGATCTGGCCGGTGCTGCGTAACGTGGAGACT
AACTTCCTCGGCTTCCTCCTCCCTCTGCTTATTATGTCCTACTGTTATTTTCGCATCATC
CAAACACTCTTCTCTTGTAAGAACCACAAGAAGGCTAAAGCTATCAAGCTGATCCTCCTG
GTGGTTATTGTGTTCTTCCTGTTCTGGACCCCGTACAATGTGATGATCTTCCTTGAAACT
CTTAAGCTGTACGACTTCTTCCCGTCCTGTGACATGCGTAAGGACCTGCGTCTGGCTCTC
TCCGTGACGGAAACGGTGGCATTCTCTCACTGCTGCCTTAACCCGCTCATCTACGCTTTC
GCCGGGGAGAAGTTTCGCCGCTACCTGTACCACCTGTACGGCAAGTGTCTCGCCGTCCTG
TGCGGCCGTTCTGTGCATGTTGACTTTTCATCCTCAGAGAGCCAGCGCTCTCGCCACGGC
TCAGTCCTGTCCTCAAACTTTACCTACCATACATCCGATGGAGACGCTCTCCTGCTCCTG
CACCACCATCACCATCACCATCACCATCACTGAAAGCTTCTCGAG

Protein sequence predicted based on nucleotide sequence

MKTIIALSYIFCLVFADYKDDDDMDQFPESVTENFEYDDLAEACYIGDIVVFGTVFLSIF
YSVIFAIGLVGNLLVVFALTNSKKPKSVTDIYLLNLALSDLLFVATLPFWTHYLINEKGL
HNAMCKFTTAFFFIGFFGSIFFITVISIDRYLAIVLAANSMNNRTVQHGVTISLGVWAAA
ILVAAPQFMFTKQKENECLGDYPEVLQEIWPVLRNVETNFLGFLLPLLIMSYCYFRIIQT
LFSCKNHKKAKAIKLILLVVIVFFLFWTPYNVMIFLETLKLYDFFPSCDMRKDLRLALSV
TETVAFSHCCLNPLIYAFAGEKFRRYLYHLYGKCLAVLCGRSVHVDFSSSESQRSRHGSV
LSSNFTYHTSDGDALLLLHHHHHHHHHH*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: kanamycin

Bacterial Selection Condition: kanamycin

Growth Condition: Growth in 30 ug/ml of kanamycin in LB at 37 degrees

Comments: None

Protein Expression Results:

ProteinExpressed: Not_Applicable

ProteinPurified: Not_Applicable

SolubleProtein: Not_Applicable

Find experimental protocols in ATCG3D_10

Recommended expression in:

Not Applicable

Vector Name:

pJ2

Synonyms:

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Cloning vector; kanamycin resistance in bacteria; restriction enzyme cloning.

Comments:

Supplied by DNA2.0

Size (bp):

2213

Parent Vector:

None

Properties:

TOPO Cloning

Author Name:

DNA2.0
Accelerated Technologies Center for Gene to 3D Structure

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

CTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGTACAAGATAAAATAATATCATCATGAACAATAAAACTGTCTGCTTACATAAACAGTAATACAAGGGGTGTTATGAGCCATATTCAACGGGAAACGTCGAGGCCGCGATTAAATTCCAACATGGATGCTGATTTATATGGGTATAAATGGGCTCGCGATAATGTCGGGCAATCAGGTGCGACAATCTATCGCTTGTATGGGAAGCCCGATGCGCCAGAGTTGTTTCTGAAACATGGCAAAGGTAGCGTTGCCAATGATGTTACAGATGAGATGGTCAGACTAAACTGGCTGACGGAATTTATGCCACTTCCGACCATCAAGCATTTTATCCGTACTCCTGATGATGCATGGTTACTCACCACTGCGATCCCCGGAAAAACAGCGTTCCAGGTATTAGAAGAATATCCTGATTCAGGTGAAAATATTGTTGATGCGCTGGCAGTGTTCCTGCGCCGGTTGCACTCGATTCCTGTTTGTAATTGTCCTTTTAACAGCGATCGCGTATTTCGCCTCGCTCAGGCGCAATCACGAATGAATAACGGTTTGGTTGATGCGAGTGATTTTGATGACGAGCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGAAATGCATAAACTTTTGCCATTCTCACCGGATTCAGTCGTCACTCATGGTGATTTCTCACTTGATAACCTTATTTTTGACGAGGGGAAATTAATAGGTTGTATTGATGTTGGACGAGTCGGAATCGCAGACCGATACCAGGATCTTGCCATCCTATGGAACTGCCTCGGTGAGTTTTCTCCTTCATTACAGAAACGGCTTTTTCAAAAATATGGTATTGATAATCCTGATATGAATAAATTGCAGTTTCATTTGATGCTCGATGAGTTTTTCTAATCAGAATTGGTTAATTGGTTGTAACACTGGCAGAGCATTACGCTGACTTGACGGGACGGCGCAAGCTCATGACCAAAATCCCTTAACGTGAGTTACGCGCGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGCCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGGCGAGAGTAGGGAACTGCCAGGCATCAAACTAAGCAGAAGGCCCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTTCTGTGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACACATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGGCTCCCCCCCCCCACCCAGCTTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

pUC ori

Bacterial origin of replication

primer

M13 forward

M13 forward sequencing primer

2062

2077

primer

M13 reverse

M13 reverse sequencing primer

114

131

selectable marker

KanR

Kanamycin resistance gene

268

1050

Gene

Gene Symbol :

CX3CR1

Symbol Nomenclature :

CX3CR1

SYNONYM :

CCRL1; CMKBRL1; CMKDR1; GPR13; GPRV28; V28

Designation :

Full Nomenclature :

chemokine (C-X3-C motif) receptor 1

GENEID :

1524

HGNC :

2558

MIM :

601470

Vega :

OTTHUMG00000156249

Target GenBank:

NP_001328

Reference Sequence Alignment

  

HsCD00292913        ---------------GGATCCATGAAAACGATCATCGCCCTGAGCTATATCTTCTGCCTT
NM_001171174.1      ATGAGAGAACCCCTGGAGGCGTTTAAGTTGGCAGACTTGGATTTCAGGAAGAGCTCTCTG
                                   *.. * :* **.: *. ..:*    : : *:. *: : **  ** 

HsCD00292913        GTTTTCGCAGACTATAAGGACGATG------ACGATATGGACCAGTTCCCTGAATCTGTG
NM_001171174.1      GCTTCTGGGTGGAGAATGGCCAGTGGGGCCTTCACCATGGATCAGTTCCCTGAATCAGTG
                    * **  * . . :.:*:**.*..**      :*.. ***** **************:***

HsCD00292913        ACGGAGAATTTCGAGTACGACGATCTGGCTGAGGCCTGTTATATTGGCGATATTGTGGTG
NM_001171174.1      ACAGAAAACTTTGAGTACGATGATTTGGCTGAGGCCTGTTATATTGGGGACATCGTGGTC
                    **.**.** ** ******** *** ********************** ** ** ***** 

HsCD00292913        TTCGGTACCGTTTTCCTCTCCATCTTTTATAGCGTGATCTTCGCCATCGGGCTGGTGGGA
NM_001171174.1      TTTGGGACTGTGTTCCTGTCCATATTCTACTCCGTCATCTTTGCCATTGGCCTGGTGGGA
                    ** ** ** ** ***** *****.** ** : *** ***** ***** ** *********

HsCD00292913        AACCTCCTCGTGGTGTTTGCCCTGACTAACTCCAAGAAGCCAAAATCCGTTACCGACATC
NM_001171174.1      AATTTGTTGGTAGTGTTTGCCCTCACCAACAGCAAGAAGCCCAAGAGTGTCACCGACATT
                    **  *  * **.*********** ** ***: *********.**.:  ** ******** 

HsCD00292913        TACCTGCTGAATCTCGCTCTGTCTGACCTGCTGTTCGTTGCTACACTGCCCTTCTGGACC
NM_001171174.1      TACCTCCTGAACCTGGCCTTGTCTGATCTGCTGTTTGTAGCCACTTTGCCCTTCTGGACT
                    ***** ***** ** **  ******* ******** **:** **: ************* 

HsCD00292913        CATTACCTGATCAATGAAAAAGGCCTCCACAACGCCATGTGTAAATTTACCACAGCTTTC
NM_001171174.1      CACTATTTGATAAATGAAAAGGGCCTCCACAATGCCATGTGCAAATTCACTACCGCCTTC
                    ** **  ****.********.*********** ******** ***** ** **.** ***

HsCD00292913        TTTTTCATCGGGTTCTTCGGTTCCATCTTCTTCATTACCGTCATTAGTATCGACCGTTAC
NM_001171174.1      TTCTTCATCGGCTTTTTTGGAAGCATATTCTTCATCACCGTCATCAGCATTGATAGGTAC
                    ** ******** ** ** **:: ***.******** ******** ** ** ** .* ***

HsCD00292913        CTGGCCATTGTGCTGGCCGCTAACTCCATGAATAACCGCACTGTTCAGCACGGTGTGACA
NM_001171174.1      CTGGCCATCGTCCTGGCCGCCAACTCCATGAACAACCGGACCGTGCAGCATGGCGTCACC
                    ******** ** ******** *********** ***** ** ** ***** ** ** **.

HsCD00292913        ATCTCCCTGGGCGTCTGGGCCGCAGCTATTCTGGTGGCCGCCCCTCAGTTCATGTTCACA
NM_001171174.1      ATCAGCCTAGGCGTCTGGGCAGCAGCCATTTTGGTGGCAGCACCCCAGTTCATGTTCACA
                    ***: ***.***********.***** *** *******.**.** ***************

HsCD00292913        AAACAAAAAGAGAACGAGTGTCTCGGCGACTACCCTGAGGTGCTCCAGGAGATCTGGCCG
NM_001171174.1      AAGCAGAAAGAAAATGAATGCCTTGGTGACTACCCCGAGGTCCTCCAGGAAATCTGGCCC
                    **.**.*****.** **.** ** ** ******** ***** ********.******** 

HsCD00292913        GTGCTGCGTAACGTGGAGACTAACTTCCTCGGCTTCCTCCTCCCTCTGCTTATTATGTCC
NM_001171174.1      GTGCTCCGCAATGTGGAAACAAATTTTCTTGGCTTCCTACTCCCCCTGCTCATTATGAGT
                    ***** ** ** *****.**:** ** ** ********.***** ***** ******:  

HsCD00292913        TACTGTTATTTTCGCATCATCCAAACACTCTTCTCTTGTAAGAACCACAAGAAGGCTAAA
NM_001171174.1      TATTGCTACTTCAGAATCATCCAGACGCTGTTTTCCTGCAAGAACCACAAGAAAGCCAAA
                    ** ** ** ** .*.********.**.** ** ** ** **************.** ***

HsCD00292913        GCTATCAAGCTGATCCTCCTGGTGGTTATTGTGTTCTTCCTGTTCTGGACCCCGTACAAT
NM_001171174.1      GCCATTAAACTGATCCTTCTGGTGGTCATCGTGTTTTTCCTCTTCTGGACACCCTACAAC
                    ** ** **.******** ******** ** ***** ***** ********.** ***** 

HsCD00292913        GTGATGATCTTCCTTGAAACTCTTAAGCTGTACGACTTCTTCCCGTCCTGTGACATGCGT
NM_001171174.1      GTTATGATTTTCCTGGAGACGCTTAAGCTCTATGACTTCTTTCCCAGTTGTGACATGAGG
                    ** ***** ***** **.** ******** ** ******** ** :  *********.* 

HsCD00292913        AAGGACCTGCGTCTGGCTCTCTCCGTGACGGAAACGGTGGCATTCTCTCACTGCTGCCTT
NM_001171174.1      AAGGATCTGAGGCTGGCCCTCAGTGTGACTGAGACGGTTGCATTTAGCCATTGTTGCCTG
                    ***** ***.* ***** ***:  ***** **.***** ***** :  ** ** ***** 

HsCD00292913        AACCCGCTCATCTACGCTTTCGCCGGGGAGAAGTTTCGCCGCTACCTGTACCACCTGTAC
NM_001171174.1      AATCCTCTCATCTATGCATTTGCTGGGGAGAAGTTCAGAAGATACCTTTACCACCTGTAT
                    ** ** ******** **:** ** *********** .*..*.***** *********** 

HsCD00292913        GGCAAGTGTCTCGCCGTCCTGTGCGGCCGTTCTGTGCATGTTGACTTTTCATCCTCAGAG
NM_001171174.1      GGGAAATGCCTGGCTGTCCTGTGTGGGCGCTCAGTCCACGTTGATTTCTCCTCATCTGAA
                    ** **.** ** ** ******** ** ** **:** ** ***** ** **.**.**:**.

HsCD00292913        AGCCAGCGCTCTCGCCACGGCTCAGTCCTGTCCTCAAACTTTACCTACCATACATCCGAT
NM_001171174.1      TCACAAAGGAGCAGGCATGGAAGTGTTCTGAGCAGCAATTTTACTTACCACACGAGTGAT
                    : .**..* :  .* ** **.: :** ***: *: .** ***** ***** **.:  ***

HsCD00292913        GGAGACGCTCTCCTGCTCCTGCACCACCATCACCATCACCATCACCATCACTGAAAGCTT
NM_001171174.1      GGAGATGCATTGCTCCTTCTCTGA------------------------------------
                    ***** **: * ** ** **  ..                                    

HsCD00292913        CTCGAG
NM_001171174.1      ------

Pathways

Panther :

Inflammation mediated by chemokine and cytokine signaling pathway

Reactome :

Chemokine receptors bind chemokines

Reactome :

Class A/1 (Rhodopsin-like receptors)

Reactome :

GPCR ligand binding

Reactome :

Peptide ligand-binding receptors

Reactome :

Signal Transduction

Reactome :

Signaling by GPCR

WikiPathway :

GPCRs, Class A Rhodopsin-like

WikiPathway :

Peptide GPCRs

Protein

Structural Annotation Wiki :

TOPSAN

UniProt :

P49238

HPRD :

03277

Clone

Cloning Information :

485277

HIP Master Clone ID :

401834

Original Clone ID :

ATCG3D_10

Experimental

TargetTrack - Experimental data :

ATCG3D_10

Organism

Species Specific ID:

1524

Genome

Chromosome :

Map Location :

3p21.3

Ensembl :

ENSG00000168329

Reagents

Labome :

CX3CR1-antibody