Gene:
Gene Symbol:
Map4k4
Gene Name:
mitogen-activated protein kinase kinase kinase kinase 4
Original Clone ID:
FLH481104.01X
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00294849
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 3705nts
Open reading frame: 35 to 3739
GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGGCGAACGACTCTCCCGCGAAGAG
CCTGGTGGACATTGACCTGTCGTCCCTGCGGGACCCTGCTGGGATTTTTGAGCTGGTGGA
AGTGGTTGGAAATGGCACCTATGGACAAGTCTATAAGGGTCGACATGTTAAAACGGGTCA
GCTGGCCGCCATCAAGGTTATGGACGTCACCGAGGATGAAGAGGAAGAAATCAAACTGGA
GATAAATATGCTGAAGAAGTATTCTCATCATCGAAATATTGCCACGTACTATGGTGCTTT
CATTAAGAAGAGCCCTCCAGGACATGATGACCAACTCTGGCTTGTTATGGAGTTTTGTGG
GGCTGGGTCCATCACAGACCTTGTGAAGAACACCAAAGGGAACACTCTCAAAGAAGACTG
GATTGCTTACATCTCCAGGGAAATCCTCAGGGGATTGGCACATCTCCATATTCACCACGT
TATTCACCGAGATATCAAGGGCCAAAATGTGCTGCTGACCGAGAATGCTGAGGTGAAACT
TGTTGATTTTGGTGTAAGCGCTCAGCTGGACAGGACGGTTGGACGGAGAAATACGTTCAT
AGGCACACCCTACTGGATGGCTCCAGAGGTCATCGCCTGTGATGAGAACCCAGACGCCAC
TTACGACTACAGAAGTGACCTCTGGTCCTGTGGCATCACAGCCATCGAGATGGCTGAAGG
GGCCCCCCCTCTCTGTGACATGCATCCAATGAGAGCGCTGTTTCTCATCCCCAGAAACCC
TCCTCCCAGGCTGAAGTCAAAAAAATGGTCAAAGAAATTTTTCAGCTTTATAGAAGGCTG
TCTGGTGAAGAATTACATGCAGCGGCCCTCTACAGAGCAACTTTTAAAACACCCTTTCAT
AAGGGATCAGCCCAATGAAAGGCAGGTTCGAATCCAGCTTAAGGATCACATAGACCGGAC
CAGAAAGAAGAGAGGCGAGAAAGATGAGACGGAGTACGAGTACAGCGGGAGCGAGGAGGA
GGAGGAGGAAGTGCCTGAGCAGGAGGGAGAGCCAAGTTCCATCGTCAATGTGCCTGGAGA
GTCAACTCTGCGACGTGATTTCCTGAGACTGCAGCAGGAGAACAAGGAGCGGTCTGAGGC
TCTGCGGAGACAGCAGCTTCTGCAGGAGCAGCAGCTCCGGGAGCAGGAGGAGTATAAGAG
GCAGCTGCTGGCTGAGAGGCAGAAGCGGATTGAACAGCAGAAAGAACAGAGGAGGCGGCT
GGAAGAGCAACAAAGAAGAGAACGGGAAGCCAGGAGGCAGCAGGAGCGTGAGCAGCGGCG
GCGTGAACAAGAGGAGAAGAGGCGTCTCGAGGAACTGGAAAGGCGGCGTAAAGAAGAGGA
AGAGAGGAGACGGGCAGAAGAGGAGAAGAGGAGAGTGGAGAGGGAACAGGAGTACATCAG
GCGGCAGCTAGAGGAGGAGCAGCGGCACCTGGAGATCCTGCAGCAGCAGCTGCTCCAGGA
GCAGGCCATGTTACTGCACGACCACAGGAGGCCGCACGCACAGCAGCAGCCGCCGCCCCC
GCAGCAGCAGGACAGGAGCAAACCGAGCTTTCATGCTCCAGAGCCCAAGCCTCACTATGA
CCCTGCTGACAGAGCTCGGGAGGTACAGTGGTCCCACCTGGCATCTCTCAAGAACAATGT
CTCCCCTGTCTCGAGATCCCATTCCTTCAGTGACCCTTCTCCCAAATTCGCACACCACCA
TCTCCGCTCTCAGGACCCATGTCCACCTTCCCGCAGTGAGGGGCTCAGTCAGAGCTCTGA
CTCTAAGTCGGAGGTGCCCGAGCCCACCCAAAAGGCTTGGTCTAGATCAGACAGTGACGA
GGTGCCTCCAAGGGTTCCCGTGAGAACGACCTCTCGTTCCCCTGTCCTCTCCCGTCGGGA
TTCCCCACTGCAGGGCGGTGGACAGCAGAATAGCCAAGCAGGACAGAGAAATTCCACCAG
CAGTATTGAGCCCCGGCTTCTTTGGGAGAGAGTGGAAAAGCTGGTCCCCAGGCCAGGCAG
TGGCAGCTCTTCAGGGTCCAGCAACTCAGGATCCCAGCCTGGCTCCCATCCTGGGTCTCA
GAGCGGCTCCGGTGAACGCTTCAGAGTGAGATCATCATCCAAGTCTGAAGGTTCTCCATC
GCCACGCCAGGAAAGTGCAGCCAAAAAGCCTGACGATAAGAAAGAAGTATTCCGGCCTCT
CAAGCCTGCGGGAGAAGTGGACTTGACTGCGTTGGCCAAAGAGCTTCGAGCAGTGGAAGA
TGTTCGGCCACCTCACAAAGTAACAGACTACTCATCCTCCAGTGAGGAGTCTGGGACCAC
AGATGAGGAGGAGGAGGACGTGGAACAGGAGGGGGCTGATGATTCTACCTCGGGACCAGA
AGACACCAGAGCAGCGTCATCTCCGAACCTGAGCAACGGTGAAACAGAATCTGTGAAAAC
AATGATCGTTCATGATGATGTAGAAAGTGAGCCAGCCATGACCCCGTCCAAGGAGGGCAC
CCTCATCGTCCGCCAGACTCAGTCCGCTAGTAGCACACTCCAGAAACACAAATCTTCCTC
CTCCTTTACACCTTTTATAGACCCCAGGTTACTACAGATCTCTCCATCTAGTGGGACGAC
AGTGACTTCCGTGGTGGGATTTTCCTGTGATGGGCTGAGACCAGAAGCCATAAGGCAAGA
TCCTACTCGGAAAGGCTCAGTGGTCAATGTGAACCCCACGAACACTAGGCCACAGAGTGA
TACCCCCGAGATTCGTAAATACAAGAAGAGATTTAACTCAGAGATCCTGTGTGCTGCCTT
ATGGGGAGTGAACTTGCTGGTTGGTACAGAGAGCGGTCTGATGCTCTTGGACAGAAGTGG
CCAAGGGAAAGTATACCCTCTGATCAGCCGAAGACGGTTCCAGCAAATGGATGTGCTCGA
AGGCCTAAATGTCCTGGTGACAATATCTGGCAAAAAGGATAAGTTACGTGTCTACTATTT
ATCCTGGCTAAGAAATAAGATTCTTCATAATGATCCAGAGGTTGAGAAGAAGCAGGGGTG
GACCACTGTGGGCGACTTGGAAGGGTGCGTGCACTATAAAGTCGTAAAATATGAAAGAAT
CAAGTTTCTGGTAATTGCTTTGAAGAGTTCTGTGGAAGTCTATGCATGGGCACCGAAGCC
ATATCACAAATTTATGGCCTTTAAGTCATTTGGAGAACTGCTACATAAGCCATTACTGGT
GGATCTCACTGTGGAGGAAGGCCAGAGGTTGAAAGTGATCTATGGATCCTGTGCGGGATT
CCATGCTGTTGATGTGGATTCAGGCTCAGTCTATGACATTTATCTACCAACACATATTCA
GTGTAGCATCAAACCCCATGCAATCATTATCCTCCCCAACACGGATGGGATGGAGCTGCT
GGTGTGCTACGAAGACGAGGGGGTTTACGTTAACACGTACGGAAGGATCACCAAGGACGT
GGTTCTGCAGTGGGGAGAGATGCCGACATCTGTAGCATATATCCGGTCGAATCAGACAAT
GGGCTGGGGAGAGAAGGCAATAGAGATACGATCTGTGGAAACTGGTCACTTGGATGGTGT
ATTTATGCACAAAAGGGCTCAGAGACTAAAGTTCCTGTGTGAACGCAATGACAAGGTGTT
CTTTGCCTCCGTCCGCTCTGGTGGCAGCAGCCAGGTCTATTTCATGACCTTAGGCAGGAC
TTCTCTTCTGAGCTGGTAGGGCCTCATGGGCCCAGCTTTCTTGTAC
Protein sequence predicted based on nucleotide sequence
MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTE DEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNT KGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDR TVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMR ALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRI QLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQ QENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREAR RQQEREQRRREQEEKRRLEELERRRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQRHLE ILQQQLLQEQAMLLHDHRRPHAQQQPPPPQQQDRSKPSFHAPEPKPHYDPADRAREVQWS HLASLKNNVSPVSRSHSFSDPSPKFAHHHLRSQDPCPPSRSEGLSQSSDSKSEVPEPTQK AWSRSDSDEVPPRVPVRTTSRSPVLSRRDSPLQGGGQQNSQAGQRNSTSSIEPRLLWERV EKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSPRQESAAKKPD DKKEVFRPLKPAGEVDLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEEEDVEQEG ADDSTSGPEDTRAASSPNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQTQSASS TLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGLRPEAIRQDPTRKGSVVNVN PTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLISRR RFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVH YKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELLHKPLLVDLTVEEGQRLK VIYGSCAGFHAVDVDSGSVYDIYLPTHIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVN TYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKF LCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
318
End on reference sequence
4022
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
