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Nod2 (Mus musculus) in pENTR223.1 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  Nod2
Gene Name:  nucleotide-binding oligomerization domain containing 2
Sequence             
Original Clone ID: FLH481124.01X
Keyword: None
Species: Mus musculus
Type: cDNA
Vector Name: pENTR223.1               Format:  CLOSED
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
MmCD00294869

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 3042nts         Open reading frame : 35 to 3042

GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGTGCTCACAGGAAGAGTTCCAGGC
ACAGAGGAGCCAGCTGGTGGCACTGCTGATCTCAGGGTCCTTGGAGGGCTTTGAGAGCAT
CTTAGACTGGCTGCTGTCTTGGGATGTGCTCTCCAGGGAGGACTATGAGGGCCTCAGCCT
CCCTGGCCAACCTCTCTCCCATTCTGCCAGGCGTCTGCTGGACACAGTCTGGAACAAGGG
TGTTTGGGGCTGTCAGAAGCTCCTCGAAGCTGTGCAGGAGGCACAGGCCAACAGTCATAC
CTTTGAACTGTATGGGTCCTGGGACACTCACTCCCTTCATCCAACCAGAGACCTGCAGAG
TCACCGGCCAGCCATTGTGAGGAGACTCTACAACCATGTAGAAGCCATGCTGGAGCTGGC
AAGGGAGGGGGGGTTTCTGAGCCAGTACGAGTGTGAGGAGATCAGGCTGCCAATCTTCAC
GTCGTCTCAGAGGGCAAGAAGGCTGCTTGATCTCGCTGCGGTGAAGGCCAATGGACTGGC
TGCCTTCCTTCTACAGCACGTCAGGGAACTACCAGCTCCACTGCCTCTGCCTTACGAGGC
TGCTGAGTGTCAGAAGTTCATATCTAAGCTGAGGACCATGGTGTTGACTCAGTCTCGCTT
CCTCAGTACTTACGATGGGTCGGAGAATCTTTGCCTGGAGGATATATACACGGAGAACAT
CTTGGAGCTGCAGACTGAAGTGGGCACAGCCGGGGCCTTGCAGAAGAGCCCTGCCATCCT
GGGCCTGGAGGACCTCTTTGATACCCATGGTCACCTGAACAGAGATGCCGACACCATACT
GGTGGTGGGCGAAGCAGGCAGTGGCAAGAGCACTCTTCTGCAGCGTTTGCACCTGCTGTG
GGCAACAGGGAGGAGCTTCCAGGAGTTTCTCTTCATTTTCCCATTCAGCTGCCGACAGTT
GCAATGCGTGGCCAAACCGCTGTCCCTGAGGACGCTGCTCTTTGAGCACTGCTGCTGGCC
TGATGTCGCTCAGGACGATGTCTTCCAGTTCCTTCTTGACCATCCTGACCGTGTCCTGTT
AACCTTTGATGGCTTGGACGAGTTCAAGTTCCGGTTCACCGACCGGGAGCGCCACTGCTC
TCCAATTGACCCCACGTCAGTCCAGACTCTGCTCTTCAACCTTCTCCAGGGGAACCTGCT
GAAGAATGCCTGCAAGGTGCTGACCAGCCGTCCGGATGCTGTGTCAGCGCTCCTCAGGAA
GTTCGTTCGCACAGAGTTGCAACTGAAGGGCTTCTCTGAAGAGGGCATCCAACTGTACCT
GAGAAAGCACCACCGGGAACCTGGGGTGGCAGACCGCCTCATCCAGCTGATCCAAGCCAC
CTCAGCCCTGCATGGTTTGTGCCACCTCCCTGTCTTCTCTTGGATGGTGTCCAGATGCCA
CCGGGAACTTCTGCTGCAGAACAGGGGCTTCCCAACAACCAGCACGGACATGTACCTCCT
GATCCTGCAGCATTTCCTGCTGCATGCCTCCCCTCCGGACTCCTCCCCACTCGGTCTGGG
ACCTGGACTCCTCCAAAGCCGGCTCTCCACCCTCCTGCACCTTGGCCACCTGGCTCTCCG
GGGCCTGGCCATGAGCTGCTATGTGTTCTCAGCCCAGCAGCTCCAGGCAGCTCAGGTTGA
CTCTGATGATATTTCTCTTGGTTTCCTGGTACGTGCCCAAAGTAGTGTGCCCGGGAGCAA
GGCGCCCCTGGAGTTCCTGCACATTACCTTCCAGTGCTTTTTTGCCGCTTTCTACTTGGC
TGTCAGTGCTGACACATCGGTGGCCTCTCTCAAGCACCTTTTCAGCTGTGGCCGGCTGGG
CAGCTCACTGCTGGGAAGGCTGCTGCCCAACCTGTGTATCCAGGGCTCCAGAGTCAAGAA
GGGCAGCGAAGCAGCCCTGCTGCAGAAGGCTGAGCCACACAACCTGCAAATCACAGCAGC
CTTCCTAGCAGGTCTGTTGTCCCAGCAGCATCGGGACCTGTTGGCTGCATGCCAGGTCTC
CGAGAGGGTACTGCTCCAGCGTCAGGCACGTGCCCGCTCGTGTCTGGCCCACAGCCTCCG
CGAGCACTTCCATTCCATCCCGCCTGCCGTGCCCGGTGAGACCAAGAGCATGCATGCTAT
GCCGGGCTTCATTTGGCTCATCCGTAGCCTGTACGAGATGCAGGAGGAGCAGTTGGCCCA
GGAGGCTGTCCGTCGCTTGGACATCGGGCACCTGAAGTTGACATTTTGCAGAGTGGGCCC
TGCAGAGTGTGCTGCACTGGCCTTTGTACTGCAACATCTCCAGCGGCCTGTGGCCCTACA
GCTGGATTACAACTCTGTGGGAGATGTTGGAGTGGAACAGCTGCGACCGTGCCTTGGGGT
CTGCACAGCTCTGTATTTGCGAGATAACAATATCTCAGACCGAGGTGCCCGCACGCTGGT
TGAGTGTGCTCTTCGCTGTGAGCAGCTGCAGAAACTAGCTCTCTTCAACAACAAACTCAC
GGATGCGTGCGCCTGCTCCATGGCCAAGCTCCTTGCACACAAGCAGAACTTCTTGTCCCT
GAGGGTGGGGAACAATCACATCACAGCCGCTGGAGCCGAGGTGCTGGCCCAGGGACTCAA
GAGCAACACCTCCCTGAAGTTCCTGGGGTTCTGGGGCAACAGCGTGGGTGATAAGGGCAC
CCAAGCCCTGGCTGAAGTTGTAGCCGACCACCAGAACCTAAAGTGGCTCAGCCTGGTAGG
AAACAACATTGGCAGCATGGGTGCCGAAGCCCTAGCACTGATGCTGGAGAAGAACAAGTC
ACTAGAGGAACTCTGCCTAGAGGAAAACCATATCTGTGACGAAGGGGTATATTCTCTCGC
AGAAGGACTCAAGAGAAATTCAACTTTGAAATTCTTGAAACTGTCCAACAATGGCATCAC
CTACCGGGGTGCAGAAGCCCTCCTGCAGGCCCTCAGCAGGAACAGTGCCATTCTGGAGGT
TTGGCTTCGAGGGAACACATTCTCTTTGGAGGAAATCCAAACACTGAGCTCCAGGGACGC
CAGACTCTTGTTGTAGGGCCTCATGGGCCCAGCTTTCTTGTAC


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 3113
Start on reference sequence 72
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
gtacaaaaaagcagaagGGCCGTCAAGGCCCACC

3' Linker Sequence:
TAAGGCCTCATGGgcccagctttcttg

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223.1

pENTR223.1 in advanced viewed

Synonyms: pDONR-223.1, pENTR223-star-Sfi
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp): 4311
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS MCS (Sfi sites flank the cassette present only in no insert form) 1265 1277
bacterial origin ori bacterial origin of replication 1 534
negative selection marker ccdB ccdB (no insert form only; lost in with insert form) 1312 1617
primer site M13 F M13 forward primer for insert sequencing 1113 1129
primer site T7 T7 Forward primer for insert sequencing 2902 2921
primer site M13 reverse M13 reverse primer for insert sequencing 2926 2942
recombination site attL1 attL1 recombination site 1165 1264
recombination site attL2 attL2 recombination site 2786 2885
selectable marker SpnR spectinomycin resistance gene 3154 4164
selectable marker CmR chlR (no insert form only; lost in with insert form) 1959 2618

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : Nod2
Symbol Nomenclature : Nod2
SYNONYM : ACUG; BLAU; CD; Card15; F830032C23Rik; IBD1; Nlrc2
Designation : caspase recruitment domain family, member 15|caspase recruitment domain-containing protein 15|nucleotide-binding oligomerization domain-containing protein 2
Full Nomenclature : nucleotide-binding oligomerization domain containing 2
GENEID : 257632
GI : 195934834
GenBank Accession : BC168407
Mouse Genome Informatics : 2429397
Vega : OTTMUSG00000029844
Target GenBank: NM_145857.2

 

Clone

Cloning Information : 481124
HIP Master Clone ID : 398852
IMAGE ID : 100068376
Original Clone ID : FLH481124.01X

Organism


TAX_ID : 10090
Species Specific ID: 257632

Genome

Chromosome : 8
Map Location : 8 C3|8
Ensembl : ENSMUSG00000055994

Reagents

Labome : Nod2-antibody