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Fmnl2 (Mus musculus) in pENTR223.1 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  Fmnl2
Gene Name:  formin-like 2
Sequence             
Original Clone ID: FLH481128.01X
Keyword: None
Species: Mus musculus
Type: cDNA
Vector Name: pENTR223.1               Format:  CLOSED
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
MmCD00294873

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 3252nts         Open reading frame : 35 to 3252

GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGGGCAACGCGGGGAGCATGGACTC
GCAGCAGACCGATTTCAAGGCGCACAACGTGCCATTGAAGCTGCCGATGCCCGAGCCAGG
TGAACTGGAGGAGCGCTTTGCCATCGTGCTGAATGCTATGAACCTACCTCCTGACAAAGC
CAGGCTACTGCGGCAGTATGACAATGAGAAAAAATGGGAGCTGATCTGTGATCAGGAGCG
ATTCCAGGTGAAGAACCCTCCCCATACGTACATACAGAAGCTCAAAGGCTACCTGGACCC
AGCTGTAACCAGGAAGAAATTCAGAAGGCGTGTTCAAGAATCTACACAAGTGCTAAGAGA
ACTGGAAATTTCATTAAGAACCAACCACATTGGATGGGTCAGAGAGTTTTTGAATGAAGA
AAACAAAGGCCTTGATGTTCTTGTGGAGTATTTGTCTTTTGCACAGTATGCAGTCACTTT
TGACTTTGAAAGTGTGGAAAGTACCATGGAGAGTACGGTGGACAAATCAAAGCCCTGGAG
CAGGTCCATCGAGGACCTGCACAGAGGGAGCAACCTGCCCTCACCTGTGGGCAACAGTGT
GTCCCGCTCTGGAAGACATTCTGCACTTCGATATAATACTTTGCCAAGCAGAAGAACCCT
GAAAAATTCAAGATTAGTGAGTAAGAAGGATGATGTTCACGTTTGTATCATGTGTTTACG
AGCCATCATGAATTACCAGTATGGCTTCAACATGGTCATGTCTCATCCACACGCCGTCAA
TGAGATTGCACTAAGTCTGAACAACAAAAATCCCAGAACAAAAGCTCTTGTCTTGGAGCT
GTTGGCAGCCGTTTGTCTTGTCAGAGGCGGGCATGAAATAATTTTATCAGCTTTTGATAA
CTTCAAGGAGGTCTGCGGAGAAAAGCAGCGTTTTGAGAAGCTGATGGAACATTTCAGGAA
TGAAGATAATAACATAGATTTTATGGTGGCCTCTATGCAGTTTATTAACATTGTAGTCCA
TTCAGTAGAAGACATGAACTTCAGAGTTCATCTACAATATGAATTCACCAAATTAGGCCT
GGATGAATACTTGGATAAGCTGAAACACACAGAGAGCGACAAGCTGCAAGTACAGATTCA
GGCTTACCTGGACAATGTGTTTGATGTGGGAGCTCTGTTGGAAGACGCAGAAACCAAGAA
TGCTGCCTTGGAGCGGGTGGAGGAGCTGGAAGAAAACATCTCTCATTTGTCTGAAAAGCT
GCAGGACACGGAGAACGAAGCCATGTCCAAGATCGTGGAACTGGAAAAGCAACTTATGCA
GAGGAATAAGGAACTGGATGTTGTCCGGGAAATCTACAAAGATGCAAATACCCAAGTTCA
CACGTTAAGAAAAATGGTCAAAGAAAAGGAAGAAGCCATTCAAAGACAGTCTACCCTGGA
AAAAAAGATTCACGAACTGGAGAAACAAGGGACCATTAAAATTCAGAAGAAAGGGGATGG
GGACATTGCCATACTGCCAGTCATGGCCTCTGGCACGTTGTCCACGGGGTCAGAACTAGC
AGTGGGTAACTATGTGGGATCAGTTCCAGGGGCCACCACCTCAGGACCCTCAGTCCCTCC
TCCCCCACCACTACCTCCATCATCAGACACATCTGAAGCAGCACAAAATGGAACAGCATC
GCCACCTATGTCCCCACCTCCTCCCCCACCCCCTCCACCCCCTCCCCCACCCCCTCCACC
CCCTCCACTCCCAGGCCCTGCAGCTGAGACTTCACCAGCTCCTCCTCTGCCACCACCCCC
ACCTCCCTCTGCACCCCCGCTGCCTGGGACTTCTTCACCTACAGTGGTTTTCAACTCAGG
ATTAGCAGCTGTGAAAATTAAGAAGCCAATCAAGACGAAGTTCCGAATGCCAGTGTTTAA
CTGGGTCGCCCTGAAGCCCAATCAGATCAACGGCACAGTCTTCAATGAAATCGATGATGA
GCGGATCCTAGAGGACTTAAATGTGGACGAATTTGAAGAAATATTCAAGACAAAAGCCCA
AGGTCCTGCCATTGATCTTTCTTCAAGCAAGCAGAAAATAACACAGAAGGCATCAAGTAA
AGTGACATTATTAGAAGCAAATCGAGCCAAAAATCTTGCCATAACTTTAAGGAAAGCTGG
GAAGTCTGCGGATGAGATATGTAAAGCTATTCATGTGTTTGACTTGAAGACGCTACCGGT
TGACTTTGTTGAGTGTTTGATGAGGTTCTTGCCAACTGAGAATGAGGTCAAAGTGCTTCG
GCTGTATGAGCGAGAGAGGAAGCCCTTGGAGAACTTGTCAGACGAGGACCGTTTCATGAT
GCAGTTCAGTAAGATTGAGCGGCTGCTGCAGAAGATGACCATCATGGCCTTCATTGGGAA
CTTCACAGAGAGCATTCAGATGCTGACTCCTCAACTTCACGCAATTATAGCAGCATCCGT
CTCTATCAAGTCATCTCAAAAGCTCAAGAAGATTCTGGAGATCATCTTGGCCCTTGGAAA
CTATATGAATAGCAGCAAACGAGGAGCAGTCTATGGATTTAAACTTCAGAGTTTAGATCT
GCTCTTAGATACAAAGTCGACAGACCGAAAGCAAACACTGTTGCACTATATATCAAATGT
GGTAAAAGAAAAATATCAACAAGTTACTCTGTTTTATAATGAGCTTCATTACGTGGAAAA
AGCTGCTGCAGTCTCCCTGGAGAACGTTCTGCTGGATGTGAAGGAGCTGCAGAGGGGGAT
GGATTTAACCAAGAGAGAGTATACCATGCATGACCACAACACCCTGCTGAAAGAGTTCCT
CCTTCACAACGAGGGCAAGCTGAAGAAACTGCAGGAGGACGCGAAGATTGCTCAGGATGC
CTTTGATGATGTGGTGAAATATTTTGGCGAAAATCCCAAGACAACTCCACCCTCCGTGTT
CTTCCCTGTCTTTGTTCGATTTGTGAAAGCCTACAAGCAAGCAGAAGAGGAAAATGAGCT
GAGGAAAAAGCAGGAACAAGCTCTTATGGAAAAACTGCTAGAGCAAGAAGCCCTGATGGA
GCAGCAGGATGCAAAGTCTCCTTCCCATAAATCAAAGAGGCAGCAGCAAGAGTTAATTGC
GGAATTAAGAAGGCGACAAGTTAAAGATAACAGACATGTATACGAGGGAAAAGATGGCGC
CATTGAAGACATTATCACAGCCTTAAAGAAGAATAATATCACTAAATTTCCAAATGTTCA
CTCGAGGATCTTAGAAACCAACCCTACAGACGAGCCGATGCGGTAGGGCCTCATGGGCCC
AGCTTTCTTGTAC


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 3527
Start on reference sequence 276
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
gtacaaaaaagcagaagGGCCGTCAAGGCCCACC

3' Linker Sequence:
TAAGGCCTCATGGgcccagctttcttg

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223.1

pENTR223.1 in advanced viewed

Synonyms: pDONR-223.1, pENTR223-star-Sfi
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp): 4311
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS MCS (Sfi sites flank the cassette present only in no insert form) 1265 1277
bacterial origin ori bacterial origin of replication 1 534
negative selection marker ccdB ccdB (no insert form only; lost in with insert form) 1312 1617
primer site M13 F M13 forward primer for insert sequencing 1113 1129
primer site T7 T7 Forward primer for insert sequencing 2902 2921
primer site M13 reverse M13 reverse primer for insert sequencing 2926 2942
recombination site attL1 attL1 recombination site 1165 1264
recombination site attL2 attL2 recombination site 2786 2885
selectable marker SpnR spectinomycin resistance gene 3154 4164
selectable marker CmR chlR (no insert form only; lost in with insert form) 1959 2618

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : Fmnl2
Symbol Nomenclature : Fmnl2
SYNONYM : 5430425K04Rik; Man
Designation : formin-like domain containing protein MAN|formin-like protein 2|protein Man
Full Nomenclature : formin-like 2
GENEID : 71409
Locus Tag : RP23-282A22.1
GI : 195934836
GenBank Accession : BC168411
Mouse Genome Informatics : 1918659
Vega : OTTMUSG00000012431
Target GenBank: NM_172409.2

 

Clone

Cloning Information : 481128
HIP Master Clone ID : 398856
IMAGE ID : 100068380
Original Clone ID : FLH481128.01X

Organism


TAX_ID : 10090
Species Specific ID: 71409

Genome

Chromosome : 2
Map Location : 2 C1.1|2
Ensembl : ENSMUSG00000036053

Reagents

Labome : Fmnl2-antibody