Gene:
Gene Symbol:
WDR17
Gene Name:
WD repeat domain 17
Original Clone ID:
FLH481143.01X
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00294888
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGCTTGGATGACTTACATTTCAAATTGGTTTGAGCAAGATGATTGGTATGAAGGACTA
CAAAGAGCAAACATGTCCCAGGTAAGGCAAGTGGGATTGCTGGCTGCTGGATGTCAGCCA
TGGAACAAGGATGTATGTGCTGCCAGTGGAGACAGGTTTGCATATTGTGCGACCCTGGCT
ATCTATATTTATCAGTTGGATCACCGTTATAATGAATTCAAACTTCACGCAATTATGTCT
GAACATAAAAAAACAATCACAGCAATTTCTTGGTGTCCACATAATCCTGATCTGTTTGCA
AGTGGCAGTACTGATAATTTAGTGATCATTTGGAATGTTGCAGAACAAAAAGTCATTGCT
AAACTCGACAGTACAAAAGGGATCCCTGCTTCTCTTAGTTGGTGCTGGAATGCAGAGGAT
GTGGTGGCATTTGTTTCCCACAGAGGCCCACTGTTCATTTGGACCATCTCAGGACCAGAT
AGTGGAGTGATTGTACACAAAGATGCTCATAGCTTCTTGTCTGATATCTGTATGTTCAGA
TGGCATACACACCAAAAGGGGAAAGTTGTGTTTGGTCATATTGATGGAAGTCTATCAATT
TTTCATCCAGGTAATAAAAATCAGAAACATGTTTTGAGACCAGAATCTCTTGAAGGGACA
GATGAAGAGGATCCAGTTACGGCCTTGGAATGGGACCCACTATCTACTGATTATCTTCTA
GTGGTTAATTTGCATTATGGAATTCGCCTGGTAGATTCTGAATCACTTTCTTGCATAACA
ACATTTAATCTTCCCAGTGCAGCAGCTTCTGTACAGTGCTTAGCCTGGGTTCCCAGTGCT
CCTGGGATGTTTATAACTGGAGATTCTCAAGTGGGTGTTTTACGCATTTGGAATGTTTCA
AGAACAACACCTATTGATAATCTTAAATTAAAGAAAACAGGATTTCACTGCTTACATGTA
CTTAATTCTCCTCCAAGAAAAAAGTTTTCAGTCCAATCTCCAACCAAAAATCATTATACA
TCCTCAACAAGCGAAGCAGTTCCACCCCCAACTTTAACACAGAATCAAGCATTTTCTCTT
CCTCCTGGTCATGCAGTGTGTTGTTTCTTGGATGGTGGAGTTGGACTTTATGATATGGGA
GCTAAGAAGTGGGATTTTCTTAGAGACTTGGGACATGTGGAAACTATCTTTGACTGCAAA
TTCAAACCTGACGATCCTAATCTTTTAGCAACAGCTTCATTTGATGGCACTATAAAAGTC
TGGGATATAAACACATTAACAGCAGTGTACACATCCCCGGGTAATGAAGGTGTTATTTAT
TCCCTTTCTTGGGCTCCAGGTGGTTTAAATTGTATTGCTGGGGGAACTTCCCGAAATGGT
GCTTTTATTTGGAATGTTCAAAAGGGCAAAATTATACAACGATTTAATGAGCATGGAACA
AATGGAATATTCTGCATTGCCTGGAGTCATAAAGATTCTAAAAGAATAGCAACCTGCAGC
AGTGATGGTTTCTGTATTATTCGAACAATTGATGGTAAAGTGCTACACAAATATAAACAT
CCAGCTGCAGTGTTTGGTTGTGATTGGAGCCAAAACAATAAAGACATGATAGCCACTGGC
TGTGAAGACACAAATGTTCGTGTTTATTATGTAGCCACCAGCTCAGATCAACCATTGAAA
GTATTTAGTGGGCATACAGCAAAAGTGTTTCATGTTAAATGGTCTCCTCTGAGAGAGGGA
ATTCTTTGCAGTGGTTCTGATGATGGTACCGTTCGAATCTGGGATTATACTCAGGATGCT
TGCATCAATATTCTTAATGGACACACTGCACCTGTGAGAGGATTAATGTGGAATACTGAG
ATTCCATATCTGCTCATATCTGGCAGCTGGGACTATACTATAAAAGTATGGGACACTCGA
GAAGGAACTTGTGTGGATACTGTGTATGATCACGGTGCAGATGTATATGGTTTAACCTGC
CATCCCAGTCGCCCCTTCACTATGGCCTCTTGCTCCCGTGACTCTACAGTGAGACTCTGG
TCATTAACAGCCTTAGTCACTCCTGTACAAATAAATATTCTGGCAGACAGATCTTGGGAA
GAAATTATTGGGAACACTGATTATGCTATAGAACCAGGCACTCCTCCTCTACTGTGTGGT
AAAGTGTCAAGAGATATTAGACAGGAAATAGAAAAACTAACTGCTAATTCTCAAGTGAAA
AAACTAAGATGGTTCTCAGAATGTTTATCTCCTCCAGGTGGCAGTGACAATTTATGGAAC
TTGGTTGCTGTGATAAAAGGACAGGATGATAGCTTACTTCCTCAGAACTACTGCAAAGGA
ATAATGCACTTGAAACATCTGATTAAATTTAGAACATCTGAAGCTCAAGAACTAACAACA
GTCAAGATGTCTAAATTTGGTGGTGGTATTGGTGTACCTGCTAAAGAGGAAAGACTGAAG
GAAGCTGCTGAAATCCACTTGAGATTAGGACAAATTCAGAGATACTGTGAACTTATGGTT
GAACTTGGAGAGTGGGACAAAGCCCTGTCAATTGCACCAGGAGTCTCTGTGAAATACTGG
AAGAAGTTAATGCAGAGGAGAGCTGACCAATTAATCCAGGAAGATAAGGATGATGTCATT
CCATACTGCATAGCCATTGGTGATGTGAAAAAGCTAGTCCATTTTTTCATGTCAAGAGGT
CAGCTTAAAGAAGCTCTGCTTGTTGCACAGGCTGCTTGTGAAGGAAATATGCAGCCCTTA
CATGTTTCCGTGCCTAAAGGAGCTTCATATTCTGATGATATCTACAAGGAAGACTTTAAT
GAACTCCTGCACAAAGTCAGTAAAGAACTGGCAGAATGGTATTTTCAAGATGGTCGAGCA
GTACTAGCCGCATGTTGCCATCTTGCCATAGATAATATTGAGCTTGCTATGGCATACCTG
ATTCGCGGAAATGAACTGGAGTTGGCAGTCTGTGTGGGCACAGTACTAGGAGAGTCTGCA
GCACCAGCAACCCACTATGCCTTAGAATTACTGGCGAGAAAGTGCATGATGATTTCAGTA
TGCTTTCCATGTGTTGGATACAGTGTCCCCTTTTGTTACGTTAACAGGAATTTGGCAGCT
GATCTTCTTCTGATGATTCCTGATAATGAACTACATTTAATAAAACTCTGTGCTTTCTAC
CCAGGATGTACTGAAGAGATAAATGACCTTCATGATAAGTGTAAGCTACCCACAGTGGAA
GAATGTATGCAGTTAGCTGAGACAGCCCGTGCAGATGACAATATATTTGAAACTGTAAAA
TATTACTTGTTAAGTCAAGAACCTGAAAAAGCCCTTCCTATTGGTATTAGCTTTGTTAAA
GAATACATCAGTAGCTCAGACTGGACTTTGGATACCATATACCCTGTTCTTGACCTACTG
AGCTATATTCGTACTGAAAAATTACTCTTGCATACGTGTACTGAAGCTCGAAATGAGTTG
CTGATATTATGTGGTTACATTGGTGCATTACTGGCTATCAGAAGACAGTACCAAAGCATT
GTTCCAGCACTTTATGAGTACACAAGTCAGCTATTAAAACGTCGGGAGGTGTCAGTACCT
TTAAAAATTGAATATCTTTCTGAGGAATTGGATGCATGGAGAGCTTGCACACAGTCCACC
AACAGATCATTAGAAGACTCTCCGTATACACCCCCTTCTGATTCACAAAGAATGATTTAT
GCAACTTTATTAAAGAGACTAAAAGAAGAGTCACTGAAAGGAATTATTGGACCAGATTAT
GTGACTGGATCAAATCTTCCAAGTCATTCTGATATTCACATTTCTTGTCTTACGGGATTA
AAAATCCAGGGCCCTGTGTTTTTCCTTGAAGACGGGAAATCTGCTATCTCCTTGAATGAT
GCTTTGATGTGGGCAAAGGTGAATCCATTCTCACCTTTAGGGACTGGAATACGACTCAAT
CCATTC
Protein sequence predicted based on nucleotide sequence
MAWMTYISNWFEQDDWYEGLQRANMSQVRQVGLLAAGCQPWNKDVCAASGDRFAYCATLA IYIYQLDHRYNEFKLHAIMSEHKKTITAISWCPHNPDLFASGSTDNLVIIWNVAEQKVIA KLDSTKGIPASLSWCWNAEDVVAFVSHRGPLFIWTISGPDSGVIVHKDAHSFLSDICMFR WHTHQKGKVVFGHIDGSLSIFHPGNKNQKHVLRPESLEGTDEEDPVTALEWDPLSTDYLL VVNLHYGIRLVDSESLSCITTFNLPSAAASVQCLAWVPSAPGMFITGDSQVGVLRIWNVS RTTPIDNLKLKKTGFHCLHVLNSPPRKKFSVQSPTKNHYTSSTSEAVPPPTLTQNQAFSL PPGHAVCCFLDGGVGLYDMGAKKWDFLRDLGHVETIFDCKFKPDDPNLLATASFDGTIKV WDINTLTAVYTSPGNEGVIYSLSWAPGGLNCIAGGTSRNGAFIWNVQKGKIIQRFNEHGT NGIFCIAWSHKDSKRIATCSSDGFCIIRTIDGKVLHKYKHPAAVFGCDWSQNNKDMIATG CEDTNVRVYYVATSSDQPLKVFSGHTAKVFHVKWSPLREGILCSGSDDGTVRIWDYTQDA CINILNGHTAPVRGLMWNTEIPYLLISGSWDYTIKVWDTREGTCVDTVYDHGADVYGLTC HPSRPFTMASCSRDSTVRLWSLTALVTPVQINILADRSWEEIIGNTDYAIEPGTPPLLCG KVSRDIRQEIEKLTANSQVKKLRWFSECLSPPGGSDNLWNLVAVIKGQDDSLLPQNYCKG IMHLKHLIKFRTSEAQELTTVKMSKFGGGIGVPAKEERLKEAAEIHLRLGQIQRYCELMV ELGEWDKALSIAPGVSVKYWKKLMQRRADQLIQEDKDDVIPYCIAIGDVKKLVHFFMSRG QLKEALLVAQAACEGNMQPLHVSVPKGASYSDDIYKEDFNELLHKVSKELAEWYFQDGRA VLAACCHLAIDNIELAMAYLIRGNELELAVCVGTVLGESAAPATHYALELLARKCMMISV CFPCVGYSVPFCYVNRNLAADLLLMIPDNELHLIKLCAFYPGCTEEINDLHDKCKLPTVE ECMQLAETARADDNIFETVKYYLLSQEPEKALPIGISFVKEYISSSDWTLDTIYPVLDLL SYIRTEKLLLHTCTEARNELLILCGYIGALLAIRRQYQSIVPALYEYTSQLLKRREVSVP LKIEYLSEELDAWRACTQSTNRSLEDSPYTPPSDSQRMIYATLLKRLKEESLKGIIGPDY VTGSNLPSHSDIHISCLTGLKIQGPVFFLEDGKSAISLNDALMWAKVNPFSPLGTGIRLN PF
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
157
End on reference sequence
4125
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
