Gene:
Gene Symbol:
ATXN7
Gene Name:
ataxin 7
Original Clone ID:
FLH481186.01X
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00294931
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGTCGGAGCGGGCCGCGGATGACGTCAGGGGGGAGCCGCGCCGCGCGGCGGCGGCGGCG
GGCGGAGCAGCGGCCGCGGCCGCCCGGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCCG
CCGCCTCCGCAGCCCCAGCGGCAGCAGCACCCGCCACCGCCGCCACGGCGCACACGGCCG
GAGGACGGCGGGCCCGGCGCCGCCTCCACCTCGGCCGCCGCAATGGCGACGGTCGGGGAG
CGCAGGCCTCTGCCCAGTCCTGAAGTGATGCTGGGACAGTCGTGGAATCTGTGGGTTGAG
GCTTCCAAACTTCCTGGGAAGGACGGGACAGAATTGGACGAAAGTTTCAAGGAGTTTGGG
AAAAACCGCGAAGTCATGGGGCTCTGTCGGGAAGACATGCCAATATTTGGTTTCTGTCCA
GCCCATGATGATTTCTACTTGGTGGTGTGTAACGACTGTAATCAGGTTGTCAAACCGCAG
GCATTTCAATCACATTATGAAAGAAGACATAGCTCATCCAGCAAGCCGCCTTTGGCCGTT
CCTCCCACTTCAGTATTTTCCTTCTTCCCTTCTCTGTCCAAAAGCAAAGGAGGCAGTGCA
AGTGGAAGCAACCGTTCTTCCAGTGGAGGTGTTCTTAGCGCATCCTCATCAAGTTCCAAG
TTGTTGAAATCACCCAAAGAGAAACTGCAGCTCAGGGGGAACACCAGGCCAATGCATCCC
ATTCAGCAAAGTAGAGTTCCCCATGGTAGAATCATGACACCCTCTGTGAAAGTGGAAAAG
ATTCATCCGAAAATGGATGGCACACTACTGAAATCTGCGGTGGGGCCAACCTGTCCTGCT
ACTGTGAGTTCCTTAGTCAAGCCTGGCCTTAACTGCCCCTCAATACCAAAGCCAACCTTG
CCTTCACCTGGACAGATTCTGAATGGCAAAGGGCTTCCTGCACCGCCCACTCTGGAAAAG
AAACCTGAAGACAATTCCAATAATAGGAAATTTTTAAATAAGAGATTATCAGAAAGAGAG
TTTGATCCTGACATCCACTGTGGGGTTATTGATCTCGACACCAAGAAGCCCTGCACCCGG
TCTTTGACATGCAAGACACATTCCTTAACCCAGCGCAGGGCTGTCCAGGGTAGAAGAAAA
CGATTTGATGTGTTATTAGCCGAGCACAAAAACAAAACCAGGGAAAAGGAATTGATTCGC
CATCCGGACTCTCAGCAACCACCGCAGCCTCTCAGGGACCCGCATCCCGCCCCTCCTAGA
ACGTCACAGGAGCCGCACCAAAACCCTCACGGAGTGATTCCTTCCGAATCAAAGCCTTTT
GTAGCTAGTAAACCTAAACCTCACACCCCCAGTCTTCCAAGGCCTCCAGGCTGCCCTGCT
CAGCAAGGTGGGAGTGCCCCCATTGACCCTCCTCCAGTCCATGAATCTCCACACCCTCCC
CTGCCTGCCACTGAGCCAGCTTCTCGGTTATCCAGTGAGGAGGGCGAAGGCGATGACAAA
GAAGAGTCTGTTGAAAAACTGGACTGTCATTATTCAGGTCATCATCCTCAGCCAGCATCT
TTTTGCACATTTGGGAGCCGGCAGATAGGAAGAGGCTATTACGTGTTTGACTCCAGGTGG
AATCGACTTCGCTGCGCCCTCAACCTCATGGTGGAGAAGCATCTGAATGCACAGCTATGG
AAGAAAATCCCACCAGTGCCCAGTACCACCTCACCCATCTCCACACGTATTCCTCACCGG
ACAAACTCTGTGCCGACATCACAATGTGGAGTCAGCTATCTGGCAGCAGCCACCGTCTCT
ACATCCCCAGTCCTGCTCTCATCTACCTGCATCTCCCCAAATAGCAAATCGGTACCAGCT
CATGGAACCACACTAAATGCACAGCCTGCTGCTTCAGGGGCGATGGATCCTGTGTGCAGT
ATGCAATCCAGACAAGTGTCCTCTTCATCCTCATCCCCTTCCACGCCCTCTGGCCTTTCC
TCGGTTCCTTCCTCCCCCATGTCCAGGAAACCTCAGAAATTGAAATCCAGCAAATCTTTG
AGGCCCAAGGAGTCTTCTGGTAACAGCACTAACTGTCAAAATGCCAGTAGCAGTACCAGT
GGCGGCTCAGGAAAGAAACGCAAAAACAGTTCCCCACTGTTGGTTCACTCTTCCTCCTCC
TCTTCCTCCTCCTCCTCTTCTTCTCATTCCATGGAGTCTTTTAGGAAAAACTGTGTGGCT
CACTCTGGGCCTCCCTACCCCTCAACGGTAACATCTTCCCATAGCATCGGCCTCAACTGT
GTGACGAATAAAGCAAATGCGGTGAACGTCCGGCATGACCAGTCAGGGAGGGGCCCCCCC
ACCGGGAGCCCTGCTGAATCCATCAAGAGGATGAGTGTGATGGTGAACAGCAGTGATTCT
ACTCTTTCTCTTGGGCCATTCATTCACCAGTCCAATGAACTGCCTGTCAACTCCCACGGC
AGTTTTTCCCACTCACACACTCCTCTAGACAAACTCATAGGAAAGAAAAGAAAGTGCTCA
CCCAGCTCGAGCAGCATCAACAACAGCAGCAGCAAACCCACAAAGGTTGCCAAAGTGCCA
GCCGTGAACAATGTCCACATGAAACACACAGGCACCATCCCAGGGGCACAAGGACTGATG
AACAGTTCCCTCCTTCATCAGCCAAAGGCACGTCCC
Protein sequence predicted based on nucleotide sequence
MSERAADDVRGEPRRAAAAAGGAAAAAARQQQQQQQQQQPPPPQPQRQQHPPPPPRRTRP EDGGPGAASTSAAAMATVGERRPLPSPEVMLGQSWNLWVEASKLPGKDGTELDESFKEFG KNREVMGLCREDMPIFGFCPAHDDFYLVVCNDCNQVVKPQAFQSHYERRHSSSSKPPLAV PPTSVFSFFPSLSKSKGGSASGSNRSSSGGVLSASSSSSKLLKSPKEKLQLRGNTRPMHP IQQSRVPHGRIMTPSVKVEKIHPKMDGTLLKSAVGPTCPATVSSLVKPGLNCPSIPKPTL PSPGQILNGKGLPAPPTLEKKPEDNSNNRKFLNKRLSEREFDPDIHCGVIDLDTKKPCTR SLTCKTHSLTQRRAVQGRRKRFDVLLAEHKNKTREKELIRHPDSQQPPQPLRDPHPAPPR TSQEPHQNPHGVIPSESKPFVASKPKPHTPSLPRPPGCPAQQGGSAPIDPPPVHESPHPP LPATEPASRLSSEEGEGDDKEESVEKLDCHYSGHHPQPASFCTFGSRQIGRGYYVFDSRW NRLRCALNLMVEKHLNAQLWKKIPPVPSTTSPISTRIPHRTNSVPTSQCGVSYLAAATVS TSPVLLSSTCISPNSKSVPAHGTTLNAQPAASGAMDPVCSMQSRQVSSSSSSPSTPSGLS SVPSSPMSRKPQKLKSSKSLRPKESSGNSTNCQNASSSTSGGSGKKRKNSSPLLVHSSSS SSSSSSSSHSMESFRKNCVAHSGPPYPSTVTSSHSIGLNCVTNKANAVNVRHDQSGRGPP TGSPAESIKRMSVMVNSSDSTLSLGPFIHQSNELPVNSHGSFSHSHTPLDKLIGKKRKCS PSSSSINNSSSKPTKVAKVPAVNNVHMKHTGTIPGAQGLMNSSLLHQPKARP
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence
3232
Start on reference sequence
554
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
