Gene:
Gene Symbol:
PTPRT
Gene Name:
protein tyrosine phosphatase, receptor type, T
Original Clone ID:
FLH481203.01X
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00294948
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGCGAGCCTCGCCGCGCTCGCCCTCAGCCTGCTCCTGAGGCTGCAGCTGCCGCCACTG
CCCGGCGCCCGGGCTCAGAGCGCCGCAGGTGGCTGTTCCTTTGATGAGCACTACAGCAAC
TGTGGTTATAGTGTGGCTCTAGGGACCAATGGGTTCACCTGGGAGCAGATTAACACATGG
GAGAAACCAATGCTGGACCAGGCAGTGCCCACAGGATCTTTCATGATGGTGAACAGCTCT
GGGAGAGCCTCTGGCCAGAAGGCCCACCTTCTCCTGCCAACCCTGAAGGAGAATGACACC
CACTGCATCGACTTCCATTACTACTTCTCCAGCCGTGACAGGTCCAGCCCAGGGGCCTTG
AACGTCTACGTGAAGGTGAATGGTGGCCCCCAAGGGAACCCTGTGTGGAATGTGTCCGGG
GTCGTCACTGAGGGCTGGGTGAAGGCAGAGCTCGCCATCAGCACTTTCTGGCCACATTTC
TATCAGGTGATATTTGAATCCGTCTCATTGAAGGGTCATCCTGGCTACATCGCCGTGGAC
GAGGTCCGGGTCCTTGCTCATCCATGCAGAAAAGCACCTCATTTTCTGCGACTCCAAAAC
GTGGAGGTGAATGTGGGGCAGAATGCCACATTTCAGTGCATTGCTGGTGGGAAGTGGTCT
CAGCATGACAAGCTTTGGCTCCAGCAATGGAATGGCAGGGACACGGCCCTGATGGTCACC
CGTGTGGTCAACCACAGGCGCTTCTCAGCCACAGTCAGTGTGGCAGACACTGCCCAGCGG
AGCGTCAGCAAGTACCGCTGTGTGATCCGCTCTGATGGTGGGTCTGGTGTGTCCAACTAC
GCGGAGCTGATCGTGAAAGAGCCTCCCACGCCCATTGCTCCCCCAGAGCTGCTGGCTGTG
GGGGCCACATACCTGTGGATCAAGCCAAATGCCAACTCCATCATCGGGGATGGCCCCATC
ATCCTGAAGGAAGTGGAATATCGCACCACCACAGGCACGTGGGCAGAGACCCACATAGTC
GACTCTCCCAACTATAAGCTGTGGCATCTGGACCCCGATGTTGAGTATGAGATCCGAGTG
CTCCTCACACGACCAGGTGAGGGGGGTACGGGACCGCCAGGGCCTCCCCTCACCACCAGG
ACCAAGTGTGCAGATCCGGTACATGGCCCACAGAACGTGGAAATCGTAGACATCAGAGCC
CGGCAGCTGACCCTGCAGTGGGAGCCCTTCGGCTACGCGGTGACCCGCTGCCATAGCTAC
AACCTCACCGTGCAGTACCAGTATGTGTTCAACCAGCAGCAGTACGAGGCCGAGGAGGTC
ATCCAGACCTCCTCCCACTACACCCTGCGAGGCCTGCGCCCCTTCATGACCATCCGGCTG
CGACTCTTGCTGTCTAACCCCGAGGGCCGAATGGAGAGCGAGGAGCTGGTGGTGCAGACT
GAGGAAGACGTTCCAGGAGCTGTTCCTCTAGAATCCATCCAAGGGGGGCCCTTTGAGGAG
AAGATCTACATCCAGTGGAAACCTCCCAATGAGACCAATGGGGTCATCACGCTCTACGAG
ATCAACTACAAGGCTGTCGGCTCGCTGGACCCAAGTGCTGACCTCTCGAGCCAGAGGGGG
AAAGTGTTCAAGCTCCGGAATGAAACCCACCACCTCTTTGTGGGTCTGTACCCAGGGACC
ACCTATTCCTTCACCATCAAGGCCAGCACAGCAAAGGGCTTTGGGCCCCCTGTCACCACT
CGGATTGCCACCAAAATTTCAGCTCCATCCATGCCTGAGTACGACACAGACACCCCATTG
AATGAGACAGACACGACCATCACAGTGATGCTGAAACCCGCTCAGTCCCGGGGAGCTCCT
GTCAGTGTTTATCAGCTGGTTGTCAAGGAGGAGCGACTTCAGAAGTCACGGAGGGCAGCT
GACATTATTGAGTGCTTTTCGGTGCCCGTGAGCTATCGGAATGCCTCCAGCCTCGATTCT
CTACACTACTTTGCTGCTGAGTTGAAGCCTGCCAACCTGCCTGTCACCCAGCCATTTACA
GTGGGTGACAATAAGACATACAATGGCTACTGGAACCCTCCTCTCTCTCCCCTGAAAAGC
TACAGCATCTACTTCCAGGCACTCAGCAAAGCCAATGGAGAGACCAAAATCAACTGTGTT
CGTCTGGCTACAAAAGCACCAATGGGCAGCGCCCAGGTGACCCCGGGGACTCCACTCTGC
CTCCTCACCACAGGTGCCTCCACCCAGAATTCTAACACTGTGGAGCCAGAGAAGCAGGTG
GACAACACCGTGAAGATGGCTGGCGTGATCGCTGGCCTCCTCATGTTCATCATCATTCTC
CTGGGCGTGATGCTCACCATCAAAAGGAGAAGAAATGCTTATTCCTACTCCTATTACTTG
AAGCTGGCCAAGAAGCAGAAGGAGACCCAGAGTGGAGCCCAGAGGGAGATGGGGCCTGTG
GCCTCTGCCGACAAACCCACCACCAAGCTCAGCGCCAGCCGCAATGATGAAGGCTTCTCT
TCTAGTTCTCAGGACGTCAACGGATTCACAGATGGCAGCCGCGGGGAGCTTTCCCAGCCC
ACCCTCACGATCCAGACTCATCCCTACCGCACCTGTGACCCTGTGGAGATGAGCTACCCC
CGGGACCAGTTCCAACCCGCCATCCGGGTGGCTGACTTGCTGCAGCACATCACGCAGATG
AAGAGAGGCCAGGGCTACGGGTTCAAGGAGGAATACGAGGCCTTACCAGAGGGGCAGACA
GCTTCGTGGGACACAGCCAAGGAGGATGAAAACCGCAATAAGAATCGATATGGGAACATC
ATATCCTACGACCATTCCCGGGTGAGGCTGCTGGTGCTGGATGGAGACCCGCACTCTGAC
TACATCAATGCCAACTACATTGACGGATACCATCGACCTCGGCACTACATTGCGACTCAA
GGTCCGATGCAGGAGACTGTAAAGGACTTTTGGAGAATGATCTGGCAGGAGAACTCCGCC
AGCATCGTCATGGTCACAAACCTGGTGGAAGTGGGCAGGGTGAAATGTGTGCGATACTGG
CCAGATGACACGGAGGTCTACGGAGACATTAAAGTCACCCTGATTGAAACAGAGCCCCTG
GCAGAATACGTCATACGCACCTTCACAGTCCAGAAGAAAGGCTACCATGAGATCCGGGAG
CTCCGCCTCTTCCACTTCACCAGCTGGCCTGACCACGGCGTTCCCTGCTATGCCACTGGC
CTTCTGGGCTTCGTCCGCCAGGTCAAGTTCCTCAACCCCCCGGAAGCTGGGCCCATAGTG
GTCCACTGCAGTGCTGGGGCTGGGCGGACTGGCTGCTTCATTGCCATTGACACCATGCTT
GACATGGCCGAGAATGAAGGGGTGGTGGACATCTTCAACTGCGTGCGTGAGCTCCGGGCC
CAAAGGGTCAACCTGGTACAGACAGAGGAGCAATATGTGTTTGTGCACGATGCCATCCTG
GAAGCGTGCCTCTGTGGCAACACTGCCATCCCTGTGTGTGAGTTCCGTTCTCTCTACTAC
AATATCAGCAGGCTGGACCCCCAGACAAACTCCAGCCAAATCAAAGATGAATTTCAGACC
CTCAACATTGTGACACCCCGTGTGCGGCCCGAGGACTGCAGCATTGGGCTCCTGCCCCGG
AACCATGATAAGAATCGAAGTATGGACGTGCTGCCTCTGGACCGCTGCCTGCCCTTCCTT
ATCTCAGTGGACGGAGAATCCAGCAATTACATCAACGCAGCACTGATGGATAGCCACAAG
CAGCCTGCCGCCTTCGTGGTCACCCAGCACCCTCTACCCAACACCGTGGCAGACTTCTGG
AGGCTGGTGTTCGATTACAACTGCTCCTCTGTGGTGATGCTGAATGAGATGGACACTGCC
CAGTTCTGTATGCAGTACTGGCCTGAGAAGACCTCCGGGTGCTATGGGCCCATCCAGGTG
GAGTTCGTCTCCGCAGACATCGACGAGGACATCATCCACAGAATATTCCGCATCTGTAAC
ATGGCCCGGCCACAGGATGGTTATCGTATAGTCCAGCACCTCCAGTACATTGGCTGGCCT
GCCTACCGGGACACGCCCCCCTCCAAGCGCTCTCTGCTCAAAGTGGTCCGACGACTGGAG
AAGTGGCAGGAGCAGTATGACGGGAGGGAGGGACGTACTGTGGTCCACTGCCTAAATGGG
GGAGGCCGTAGTGGAACCTTCTGTGCCATCTGCAGTGTGTGTGAGATGATCCAGCAGCAA
AACATCATTGACGTGTTCCACATCGTGAAAACACTGCGTAACAACAAATCCAACATGGTG
GAGACCCTGGAACAGTATAAATTTGTATACGAGGTGGCACTGGAATATTTAAGCTCCTTT
Protein sequence predicted based on nucleotide sequence
MASLAALALSLLLRLQLPPLPGARAQSAAGGCSFDEHYSNCGYSVALGTNGFTWEQINTW EKPMLDQAVPTGSFMMVNSSGRASGQKAHLLLPTLKENDTHCIDFHYYFSSRDRSSPGAL NVYVKVNGGPQGNPVWNVSGVVTEGWVKAELAISTFWPHFYQVIFESVSLKGHPGYIAVD EVRVLAHPCRKAPHFLRLQNVEVNVGQNATFQCIAGGKWSQHDKLWLQQWNGRDTALMVT RVVNHRRFSATVSVADTAQRSVSKYRCVIRSDGGSGVSNYAELIVKEPPTPIAPPELLAV GATYLWIKPNANSIIGDGPIILKEVEYRTTTGTWAETHIVDSPNYKLWHLDPDVEYEIRV LLTRPGEGGTGPPGPPLTTRTKCADPVHGPQNVEIVDIRARQLTLQWEPFGYAVTRCHSY NLTVQYQYVFNQQQYEAEEVIQTSSHYTLRGLRPFMTIRLRLLLSNPEGRMESEELVVQT EEDVPGAVPLESIQGGPFEEKIYIQWKPPNETNGVITLYEINYKAVGSLDPSADLSSQRG KVFKLRNETHHLFVGLYPGTTYSFTIKASTAKGFGPPVTTRIATKISAPSMPEYDTDTPL NETDTTITVMLKPAQSRGAPVSVYQLVVKEERLQKSRRAADIIECFSVPVSYRNASSLDS LHYFAAELKPANLPVTQPFTVGDNKTYNGYWNPPLSPLKSYSIYFQALSKANGETKINCV RLATKAPMGSAQVTPGTPLCLLTTGASTQNSNTVEPEKQVDNTVKMAGVIAGLLMFIIIL LGVMLTIKRRRNAYSYSYYLKLAKKQKETQSGAQREMGPVASADKPTTKLSASRNDEGFS SSSQDVNGFTDGSRGELSQPTLTIQTHPYRTCDPVEMSYPRDQFQPAIRVADLLQHITQM KRGQGYGFKEEYEALPEGQTASWDTAKEDENRNKNRYGNIISYDHSRVRLLVLDGDPHSD YINANYIDGYHRPRHYIATQGPMQETVKDFWRMIWQENSASIVMVTNLVEVGRVKCVRYW PDDTEVYGDIKVTLIETEPLAEYVIRTFTVQKKGYHEIRELRLFHFTSWPDHGVPCYATG LLGFVRQVKFLNPPEAGPIVVHCSAGAGRTGCFIAIDTMLDMAENEGVVDIFNCVRELRA QRVNLVQTEEQYVFVHDAILEACLCGNTAIPVCEFRSLYYNISRLDPQTNSSQIKDEFQT LNIVTPRVRPEDCSIGLLPRNHDKNRSMDVLPLDRCLPFLISVDGESSNYINAALMDSHK QPAAFVVTQHPLPNTVADFWRLVFDYNCSSVVMLNEMDTAQFCMQYWPEKTSGCYGPIQV EFVSADIDEDIIHRIFRICNMARPQDGYRIVQHLQYIGWPAYRDTPPSKRSLLKVVRRLE KWQEQYDGREGRTVVHCLNGGGRSGTFCAICSVCEMIQQQNIIDVFHIVKTLRNNKSNMV ETLEQYKFVYEVALEYLSSF
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
185
End on reference sequence
4567
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
