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HNRNPUL2 (Homo sapiens) in pENTR223.1 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  HNRNPUL2
Gene Name:  heterogeneous nuclear ribonucleoprotein U-like 2
Sequence             
Original Clone ID: FLH481396.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223.1               Format:  CLOSED
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
HsCD00295141

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 2244nts         Open reading frame : 35 to 2244
Reference Sequence Alignment

GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGGAGGTGAAGCGGCTGAAAGTGAC
CGAGCTGCGGTCGGAGCTGCAGCGGCGGGGCCTGGACTCGCGCGGCCTCAAGGTGGATCT
GGCGCAGCGGCTGCAGGAGGCGCTGGACGCCGAGATGCTCGAGGACGAGGCCGGCGGCGG
CGGGGCCGGGCCCGGCGGGGCCTGCAAGGCGGAGCCTCGGCCTGTGGCCGCGTCGGGCGG
CGGCCCGGGCGGGGACGAGGAGGAGGACGAAGAGGAGGAGGAGGAGGACGAGGAGGCGCT
GCTTGAGGACGAGGACGAGGAGCCACCCCCTGCTCAAGCCTTGGGTCAGGCCGCGCAGCC
GCCGCCGGAGCCCCCGGAGGCGGCAGCCATGGAGGCCGCGGCCGAGCCAGATGCTTCCGA
GAAGCCGGCGGAGGCCACGGCCGGGTCAGGCGGGGTAAATGGTGGCGAAGAGCAGGGCCT
CGGCAAGAGGGAGGAAGACGAACCCGAGGAGCGGAGCGGGGACGAGACGCCGGGATCCGA
GGTGCCGGGTGACAAGGCCGCCGAGGAACAGGGAGATGACCAGGATAGTGAAAAGTCAAA
ACCAGCAGGCTCAGATGGTGAGCGGCGGGGGGTAAAGAGACAGCGGGATGAGAAGGATGA
ACATGGCCGAGCTTACTATGAATTCCGAGAGGAGGCTTACCACAGCCGCTCAAAGTCTCC
ACTGCCTCCTGAAGAAGAGGCAAAAGATGAGGAGGAGGATCAAACTCTTGTGAACCTGGA
CACGTATACCTCGGATCTGCATTTTCAAGTGAGCAAAGACCGCTATGGAGGGCAGCCACT
TTTCTCAGAGAAGTTCCCCACCCTTTGGTCTGGGGCAAGGAGTACTTACGGAGTGACAAA
GGGAAAAGTCTGCTTTGAGGCAAAGGTAACCCAGAATCTCCCAATGAAAGAAGGCTGCAC
AGAGGTCTCTCTCCTTCGAGTTGGGTGGTCTGTTGATTTTTCCCGTCCACAGCTTGGTGA
AGATGAATTCTCTTACGGTTTCGATGGACGAGGACTCAAGGCAGAAAATGGACAATTTGA
GGAATTTGGCCAGACTTTTGGGGAGAATGATGTTATTGGCTGCTTTGCTAATTTTGAGAC
TGAAGAAGTAGAACTTTCCTTCTCCAAGAATGGAGAAGACCTAGGTGTGGCATTCTGGAT
CAGCAAGGATTCCCTGGCAGACCGGGCCCTTCTACCCCATGTCCTCTGCAAAAATTGTGT
TGTAGAATTAAACTTCGGTCAGAAGGAGGAGCCCTTCTTCCCACCACCAGAAGAGTTTGT
GTTCATTCATGCTGTGCCTGTTGAGGAGCGTGTACGCACTGCAGTCCCTCCCAAGACCAT
AGAGGAATGTGAGGTGATTCTGATGGTGGGACTACCCGGATCTGGAAAGACCCAGTGGGC
ACTGAAATATGCAAAAGAAAACCCTGAGAAAAGATACAATGTCCTGGGAGCTGAGACTGT
GCTCAATCAAATGAGGATGAAGGGTCTCGAGGAGCCAGAGATGGACCCCAAAAGCCGAGA
CCTTTTAGTTCAGCAAGCCTCCCAGTGCCTTAGTAAGCTGGTCCAGATTGCTTCCCGGAC
AAAGAGGAACTTTATTCTTGATCAGTGTAATGTGTACAATTCTGGCCAACGGCGGAAGCT
ATTGCTGTTCAAGACCTTCTCTCGGAAAGTGGTGGTGGTTGTCCCTAATGAGGAAGATTG
GAAGAAGAGGCTGGAGTTGAGGAAGGAAGTAGAGGGAGATGATGTGCCTGAATCTATAAT
GCTGGAGATGAAAGCCAACTTCTCTTTGCCTGAAAAATGCGACTATATGGATGAGGTGAC
ATATGGGGAGCTGGAGAAGGAGGAAGCTCAGCCCATTGTCACTAAGTACAAGGAGGAGGC
AAGGAAGCTTCTGCCCCCCTCCGAGAAGCGGACAAATCGCCGAAACAACCGAAACAAGCG
TAACCGGCAGAACCGAAGCCGGGGCCAAGGCTATGTGGGCGGGCAGCGCCGAGGCTACGA
CAACCGGGCCTACGGGCAGCAGTACTGGGGGCAGCCTGGAAACAGAGGGGGTTACCGTAA
TTTCTATGATCGATACAGGGGAGACTATGATCGATTTTACGGGCGAGATTATGAGTACAA
CAGATACAGAGACTATTACAGACAATACAATCGGGATTGGCAGAGTTACTACTACCACCA
CCCCCAGGACAGAGACCGATACTACAGGAATTACTACGGGTACCAAGGGTATCGGTAGGG
CCTCATGGGCCCAGCTTTCTTGTAC


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 2437
Start on reference sequence 194
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
gtacaaaaaagcagaagGGCCGTCAAGGCCCACC

3' Linker Sequence:
TAAGGCCTCATGGgcccagctttcttg

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pENTR223.1

pENTR223.1 in advanced viewed

Synonyms: pDONR-223.1, pENTR223-star-Sfi
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp): 4311
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS MCS (Sfi sites flank the cassette present only in no insert form) 1265 1277
bacterial origin ori bacterial origin of replication 1 534
negative selection marker ccdB ccdB (no insert form only; lost in with insert form) 1312 1617
primer site M13 F M13 forward primer for insert sequencing 1113 1129
primer site T7 T7 Forward primer for insert sequencing 2902 2921
primer site M13 reverse M13 reverse primer for insert sequencing 2926 2942
recombination site attL1 attL1 recombination site 1165 1264
recombination site attL2 attL2 recombination site 2786 2885
selectable marker SpnR spectinomycin resistance gene 3154 4164
selectable marker CmR chlR (no insert form only; lost in with insert form) 1959 2618

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : HNRNPUL2
Symbol Nomenclature : HNRNPUL2
SYNONYM : HNRPUL2; SAF-A2
Designation : heterogeneous nuclear ribonucleoprotein U-like protein 2|scaffold-attachment factor A2
Full Nomenclature : heterogeneous nuclear ribonucleoprotein U-like 2
GENEID : 221092
GI : 189442880
GenBank Accession : BC167844
HGNC : 25451
Vega : OTTHUMG00000167773
Target GenBank: NM_001079559.1

 

Reference Sequence Alignment

  

HsCD00295141        GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGGAGGTGAAGCGGCTGAAAGTGAC
NM_001079559.2      ----------------------------------ATGGAGGTGAAGCGGCTGAAAGTGAC
                                                      **************************

HsCD00295141        CGAGCTGCGGTCGGAGCTGCAGCGGCGGGGCCTGGACTCGCGCGGCCTCAAGGTGGATCT
NM_001079559.2      CGAGCTGCGGTCGGAGCTGCAGCGGCGGGGCCTGGACTCGCGCGGCCTCAAGGTGGATCT
                    ************************************************************

HsCD00295141        GGCGCAGCGGCTGCAGGAGGCGCTGGACGCCGAGATGCTCGAGGACGAGGCCGGCGGCGG
NM_001079559.2      GGCGCAGCGGCTGCAGGAGGCGCTGGACGCCGAGATGCTCGAGGACGAGGCCGGCGGCGG
                    ************************************************************

HsCD00295141        CGGGGCCGGGCCCGGCGGGGCCTGCAAGGCGGAGCCTCGGCCTGTGGCCGCGTCGGGCGG
NM_001079559.2      CGGGGCCGGGCCCGGCGGGGCCTGCAAGGCGGAGCCTCGGCCTGTGGCCGCGTCGGGCGG
                    ************************************************************

HsCD00295141        CGGCCCGGGCGGGGACGAGGAGGAGGACGAAGAGGAGGAGGAGGAGGACGAGGAGGCGCT
NM_001079559.2      CGGCCCGGGCGGGGACGAGGAGGAGGACGAAGAGGAGGAGGAGGAGGACGAGGAGGCGCT
                    ************************************************************

HsCD00295141        GCTTGAGGACGAGGACGAGGAGCCACCCCCTGCTCAAGCCTTGGGTCAGGCCGCGCAGCC
NM_001079559.2      GCTTGAGGACGAGGACGAGGAGCCACCCCCTGCTCAAGCCTTGGGTCAGGCCGCGCAGCC
                    ************************************************************

HsCD00295141        GCCGCCGGAGCCCCCGGAGGCGGCAGCCATGGAGGCCGCGGCCGAGCCAGATGCTTCCGA
NM_001079559.2      GCCGCCGGAGCCCCCGGAGGCGGCAGCCATGGAGGCCGCGGCCGAGCCAGATGCTTCCGA
                    ************************************************************

HsCD00295141        GAAGCCGGCGGAGGCCACGGCCGGGTCAGGCGGGGTAAATGGTGGCGAAGAGCAGGGCCT
NM_001079559.2      GAAGCCGGCGGAGGCCACGGCCGGGTCAGGCGGGGTAAATGGTGGCGAAGAGCAGGGCCT
                    ************************************************************

HsCD00295141        CGGCAAGAGGGAGGAAGACGAACCCGAGGAGCGGAGCGGGGACGAGACGCCGGGATCCGA
NM_001079559.2      CGGCAAGAGGGAGGAAGACGAACCCGAGGAGCGGAGCGGGGACGAGACGCCGGGATCCGA
                    ************************************************************

HsCD00295141        GGTGCCGGGTGACAAGGCCGCCGAGGAACAGGGAGATGACCAGGATAGTGAAAAGTCAAA
NM_001079559.2      GGTGCCGGGTGACAAGGCCGCCGAGGAACAGGGAGATGACCAGGATAGTGAAAAGTCAAA
                    ************************************************************

HsCD00295141        ACCAGCAGGCTCAGATGGTGAGCGGCGGGGGGTAAAGAGACAGCGGGATGAGAAGGATGA
NM_001079559.2      ACCAGCAGGCTCAGATGGTGAGCGGCGGGGGGTAAAGAGACAGCGGGATGAGAAGGATGA
                    ************************************************************

HsCD00295141        ACATGGCCGAGCTTACTATGAATTCCGAGAGGAGGCTTACCACAGCCGCTCAAAGTCTCC
NM_001079559.2      ACATGGCCGAGCTTACTATGAATTCCGAGAGGAGGCTTACCACAGCCGCTCAAAGTCTCC
                    ************************************************************

HsCD00295141        ACTGCCTCCTGAAGAAGAGGCAAAAGATGAGGAGGAGGATCAAACTCTTGTGAACCTGGA
NM_001079559.2      ACTGCCTCCTGAAGAAGAGGCAAAAGATGAGGAGGAGGATCAAACTCTTGTGAACCTGGA
                    ************************************************************

HsCD00295141        CACGTATACCTCGGATCTGCATTTTCAAGTGAGCAAAGACCGCTATGGAGGGCAGCCACT
NM_001079559.2      CACGTATACCTCGGATCTGCATTTTCAAGTGAGCAAAGACCGCTATGGAGGGCAGCCACT
                    ************************************************************

HsCD00295141        TTTCTCAGAGAAGTTCCCCACCCTTTGGTCTGGGGCAAGGAGTACTTACGGAGTGACAAA
NM_001079559.2      TTTCTCAGAGAAGTTCCCCACCCTTTGGTCTGGGGCAAGGAGTACTTACGGAGTGACAAA
                    ************************************************************

HsCD00295141        GGGAAAAGTCTGCTTTGAGGCAAAGGTAACCCAGAATCTCCCAATGAAAGAAGGCTGCAC
NM_001079559.2      GGGAAAAGTCTGCTTTGAGGCAAAGGTAACCCAGAATCTCCCAATGAAAGAAGGCTGCAC
                    ************************************************************

HsCD00295141        AGAGGTCTCTCTCCTTCGAGTTGGGTGGTCTGTTGATTTTTCCCGTCCACAGCTTGGTGA
NM_001079559.2      AGAGGTCTCTCTCCTTCGAGTTGGGTGGTCTGTTGATTTTTCCCGTCCACAGCTTGGTGA
                    ************************************************************

HsCD00295141        AGATGAATTCTCTTACGGTTTCGATGGACGAGGACTCAAGGCAGAAAATGGACAATTTGA
NM_001079559.2      AGATGAATTCTCTTACGGTTTCGATGGACGAGGACTCAAGGCAGAAAATGGACAATTTGA
                    ************************************************************

HsCD00295141        GGAATTTGGCCAGACTTTTGGGGAGAATGATGTTATTGGCTGCTTTGCTAATTTTGAGAC
NM_001079559.2      GGAATTTGGCCAGACTTTTGGGGAGAATGATGTTATTGGCTGCTTTGCTAATTTTGAGAC
                    ************************************************************

HsCD00295141        TGAAGAAGTAGAACTTTCCTTCTCCAAGAATGGAGAAGACCTAGGTGTGGCATTCTGGAT
NM_001079559.2      TGAAGAAGTAGAACTTTCCTTCTCCAAGAATGGAGAAGACCTAGGTGTGGCATTCTGGAT
                    ************************************************************

HsCD00295141        CAGCAAGGATTCCCTGGCAGACCGGGCCCTTCTACCCCATGTCCTCTGCAAAAATTGTGT
NM_001079559.2      CAGCAAGGATTCCCTGGCAGACCGGGCCCTTCTACCCCATGTCCTCTGCAAAAATTGTGT
                    ************************************************************

HsCD00295141        TGTAGAATTAAACTTCGGTCAGAAGGAGGAGCCCTTCTTCCCACCACCAGAAGAGTTTGT
NM_001079559.2      TGTAGAATTAAACTTCGGTCAGAAGGAGGAGCCCTTCTTCCCACCACCAGAAGAGTTTGT
                    ************************************************************

HsCD00295141        GTTCATTCATGCTGTGCCTGTTGAGGAGCGTGTACGCACTGCAGTCCCTCCCAAGACCAT
NM_001079559.2      GTTCATTCATGCTGTGCCTGTTGAGGAGCGTGTACGCACTGCAGTCCCTCCCAAGACCAT
                    ************************************************************

HsCD00295141        AGAGGAATGTGAGGTGATTCTGATGGTGGGACTACCCGGATCTGGAAAGACCCAGTGGGC
NM_001079559.2      AGAGGAATGTGAGGTGATTCTGATGGTGGGACTACCCGGATCTGGAAAGACCCAGTGGGC
                    ************************************************************

HsCD00295141        ACTGAAATATGCAAAAGAAAACCCTGAGAAAAGATACAATGTCCTGGGAGCTGAGACTGT
NM_001079559.2      ACTGAAATATGCAAAAGAAAACCCTGAGAAAAGATACAATGTCCTGGGAGCTGAGACTGT
                    ************************************************************

HsCD00295141        GCTCAATCAAATGAGGATGAAGGGTCTCGAGGAGCCAGAGATGGACCCCAAAAGCCGAGA
NM_001079559.2      GCTCAATCAAATGAGGATGAAGGGTCTCGAGGAGCCAGAGATGGACCCCAAAAGCCGAGA
                    ************************************************************

HsCD00295141        CCTTTTAGTTCAGCAAGCCTCCCAGTGCCTTAGTAAGCTGGTCCAGATTGCTTCCCGGAC
NM_001079559.2      CCTTTTAGTTCAGCAAGCCTCCCAGTGCCTTAGTAAGCTGGTCCAGATTGCTTCCCGGAC
                    ************************************************************

HsCD00295141        AAAGAGGAACTTTATTCTTGATCAGTGTAATGTGTACAATTCTGGCCAACGGCGGAAGCT
NM_001079559.2      AAAGAGGAACTTTATTCTTGATCAGTGTAATGTGTACAATTCTGGCCAACGGCGGAAGCT
                    ************************************************************

HsCD00295141        ATTGCTGTTCAAGACCTTCTCTCGGAAAGTGGTGGTGGTTGTCCCTAATGAGGAAGATTG
NM_001079559.2      ATTGCTGTTCAAGACCTTCTCTCGGAAAGTGGTGGTGGTTGTCCCTAATGAGGAAGATTG
                    ************************************************************

HsCD00295141        GAAGAAGAGGCTGGAGTTGAGGAAGGAAGTAGAGGGAGATGATGTGCCTGAATCTATAAT
NM_001079559.2      GAAGAAGAGGCTGGAGTTGAGGAAGGAAGTAGAGGGAGATGATGTGCCTGAATCTATAAT
                    ************************************************************

HsCD00295141        GCTGGAGATGAAAGCCAACTTCTCTTTGCCTGAAAAATGCGACTATATGGATGAGGTGAC
NM_001079559.2      GCTGGAGATGAAAGCCAACTTCTCTTTGCCTGAAAAATGCGACTATATGGATGAGGTGAC
                    ************************************************************

HsCD00295141        ATATGGGGAGCTGGAGAAGGAGGAAGCTCAGCCCATTGTCACTAAGTACAAGGAGGAGGC
NM_001079559.2      ATATGGGGAGCTGGAGAAGGAGGAAGCTCAGCCCATTGTCACTAAGTACAAGGAGGAGGC
                    ************************************************************

HsCD00295141        AAGGAAGCTTCTGCCCCCCTCCGAGAAGCGGACAAATCGCCGAAACAACCGAAACAAGCG
NM_001079559.2      AAGGAAGCTTCTGCCCCCCTCCGAGAAGCGGACAAATCGCCGAAACAACCGAAACAAGCG
                    ************************************************************

HsCD00295141        TAACCGGCAGAACCGAAGCCGGGGCCAAGGCTATGTGGGCGGGCAGCGCCGAGGCTACGA
NM_001079559.2      TAACCGGCAGAACCGAAGCCGGGGCCAAGGCTATGTGGGCGGGCAGCGCCGAGGCTACGA
                    ************************************************************

HsCD00295141        CAACCGGGCCTACGGGCAGCAGTACTGGGGGCAGCCTGGAAACAGAGGGGGTTACCGTAA
NM_001079559.2      CAACCGGGCCTACGGGCAGCAGTACTGGGGGCAGCCTGGAAACAGAGGGGGTTACCGTAA
                    ************************************************************

HsCD00295141        TTTCTATGATCGATACAGGGGAGACTATGATCGATTTTACGGGCGAGATTATGAGTACAA
NM_001079559.2      TTTCTATGATCGATACAGGGGAGACTATGATCGATTTTACGGGCGAGATTATGAGTACAA
                    ************************************************************

HsCD00295141        CAGATACAGAGACTATTACAGACAATACAATCGGGATTGGCAGAGTTACTACTACCACCA
NM_001079559.2      CAGATACAGAGACTATTACAGACAATACAATCGGGATTGGCAGAGTTACTACTACCACCA
                    ************************************************************

HsCD00295141        CCCCCAGGACAGAGACCGATACTACAGGAATTACTACGGGTACCAAGGGTATCGGTAGGG
NM_001079559.2      CCCCCAGGACAGAGACCGATACTACAGGAATTACTACGGGTACCAAGGGTATCGGTGA--
                    ********************************************************..  

HsCD00295141        CCTCATGGGCCCAGCTTTCTTGTAC
NM_001079559.2      -------------------------
                                             

Clone

Cloning Information : 481396
HIP Master Clone ID : 399124
IMAGE ID : 100068234
Original Clone ID : FLH481396.01X

Organism


TAX_ID : 9606
Species Specific ID: 221092

Genome

Chromosome : 11
Map Location : 11q12.3
Ensembl : ENSG00000214753

Reagents

Labome : HNRNPUL2-antibody