Gene:
Gene Symbol:
EDEM3
Gene Name:
ER degradation enhancer, mannosidase alpha-like 3
Original Clone ID:
FLH481438.01X
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00295183
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGAGTAGGGAGGAGAAACAGAAGCT
TGGGAATCAAGTACTGGAAATGTTTGATCATGCTTATGGTAACTATATGGAACATGCTTA
CCCTGCTGATGAACTCATGCCTTTAACCTGTAGAGGTCGAGTTAGAGGCCAAGAGCCAAG
TCGCGGTGACGTTGATGATGCCTTGGGAAAATTTTCTCTGACACTGATTGATTCTTTGGA
CACTCTTGTGGTTTTAAATAAAACTAAAGAATTTGAAGATGCAGTGAGAAAAGTTTTAAG
AGATGTTAATTTAGATAACGATGTAGTCGTATCAGTCTTTGAAACAAACATCAGAGTTCT
TGGGGGTCTTTTGGGTGGGCACTCCCTGGCAATCATGCTGAAAGAAAAAGGTGAATATAT
GCAGTGGTACAATGATGAACTTCTCCAAATGGCAAAGCAGTTAGGTTACAAACTTTTACC
GGCTTTCAACACTACCAGTGGCCTTCCTTATCCAAGAATTAATTTAAAGTTTGGCATCAG
AAAACCAGAAGCTCGGACAGGAACTGAGACAGATACCTGTACAGCTTGTGCAGGTACCTT
GATCCTTGAATTTGCTGCTTTAAGTCGATTCACAGGAGCAACAATATTTGAGGAATATGC
CAGAAAAGCTCTTGATTTTCTCTGGGAAAAAAGACAGCGAAGTAGTAATTTAGTGGGCGT
GACTATAAATATTCATACTGGAGATTGGGTACGAAAAGATAGTGGAGTTGGAGCAGGGAT
TGATTCATATTATGAATATCTGTTGAAAGCCTATGTCTTGCTTGGAGATGACAGTTTTCT
GGAAAGATTTAACACACACTATGATGCCATAATGAGGTATATTAGCCAGCCACCTCTTCT
ACTTGATGTGCATATCCACAAACCAATGCTGAATGCTCGGACTTGGATGGATGCTTTGCT
TGCCTTCTTCCCAGGCTTGCAGGTGTTAAAGGGGGATATTAGACCTGCTATTGAAACTCA
TGAAATGTTATATCAGGTGATTAAAAAACACAATTTTCTACCAGAGGCATTTACCACAGA
TTTCAGAGTACACTGGGCTCAACATCCTTTAAGGCCAGAATTTGCAGAAAGTACCTACTT
CTTATATAAAGCTACAGGAGATCCTTACTACCTTGAAGTAGGGAAGACACTTATTGAAAA
TTTAAATAAATATGCTAGAGTGCCTTGCGGATTTGCTGCCATGAAGGATGTTCGTACTGG
AAGTCATGAGGACAGAATGGATTCTTTCTTCTTGGCTGAAATGTTTAAATATCTTTACCT
GTTATTTGCTGATAAAGAAGACATTATTTTTGACATAGAAGATTACATCTTTACAACAGA
AGCTCATCTGTTACCTCTTTGGCTCTCTACTACAAATCAAAGCATCTCTAAAAAGAACAC
AACCTCGGAATATACAGAACTGGATGACAGTAACTTCGATTGGACTTGTCCAAATACTCA
GATCCTCTTTCCTAATGACCCATTGTATGCTCAAAGTATTCGTGAGCCCTTGAAAAATGT
GGTGGATAAGAGCTGTCCTAGAGGCATCATCAGAGTAGAGGAGAGTTTCAGGAGTGGAGC
TAAACCCCCTCTGAGAGCCAGAGATTTCATGGCCACTAACCCTGAGCATTTAGAAATCCT
GAAGAAGATGGGGGTGAGTTTGATTCACCTCAAAGATGGGAGAGTCCAGTTGGTCCAACA
TGCAATCCAAGCTGCTAGTTCAATCGACGCTGAAGATGGGTTGAGGTTCATGCAGGAGAT
GATTGAATTGTCAAGTCAGCAACAAAAAGAACAGCAGCTGCCTCCACGAGCTGTACAAAT
TGTTTCCCACCCATTTTTTGGCAGGGTAGTATTGACTGCTGGACCAGCTCAGTTTGGGCT
GGATCTGTCTAAACATAAAGAGACAAGAGGATTTGTTGCAAGCAGTAAACCATCCAATGG
TTGTTCAGAGCTTACTAACCCAGAGGCAGTGATGGGAAAAATCGCACTGATACAAAGAGG
ACAGTGCATGTTTGCAGAAAAGGCACGCAACATCCAGAATGCTGGAGCCATTGGTGGCAT
TGTTATTGATGACAATGAGGGGAGCAGCAGTGATACTGCCCCTCTGTTCCAGATGGCAGG
TGATGGAAAGGATACAGATGACATCAAGATCCCCATGCTGTTCTTATTCAGCAAAGAAGG
AAGTATCATACTGGATGCCATCCGGGAATATGAGGAGGTAGAAGTGCTCCTCTCTGATAA
AGCAAAAGATCGAGATCCTGAAATGGAAAATGAAGAACAACCATCCTCTGAAAATGATTC
TCAGAATCAGAGTGGTGAACAGATTTCATCAAGTTCTCAGGAGGTTGATTTGGTTGATCA
AGAGTCTTCTGAGGAAAATTCTCTAAATTCTCACCCAGAATCATTATCTCTAGCAGATAT
GGACAATGCTGCAAGCATTTCCCCTTCTGAACAGACTTCTAATCCCACAGAAAACCATGA
GACTACAAATCTTAATGGTGAATGTACAGATTTAGATAACCAGCTTCAAGAACAATCAGA
AACTGAGGAAGATTCCAATCCTAATGTTAGCTGGGGTAAAAAGGTCCAGCCTATAGACTC
CATATTAGCAGACTGGAATGAAGATATAGAAGCATTTGAAATGATGGAGAAGGATGAGCT
ATAGGGCCTCATGGGCCCAGCTTTCTTGTAC
Protein sequence predicted based on nucleotide sequence
MSREEKQKLGNQVLEMFDHAYGNYMEHAYPADELMPLTCRGRVRGQEPSRGDVDDALGKF SLTLIDSLDTLVVLNKTKEFEDAVRKVLRDVNLDNDVVVSVFETNIRVLGGLLGGHSLAI MLKEKGEYMQWYNDELLQMAKQLGYKLLPAFNTTSGLPYPRINLKFGIRKPEARTGTETD TCTACAGTLILEFAALSRFTGATIFEEYARKALDFLWEKRQRSSNLVGVTINIHTGDWVR KDSGVGAGIDSYYEYLLKAYVLLGDDSFLERFNTHYDAIMRYISQPPLLLDVHIHKPMLN ARTWMDALLAFFPGLQVLKGDIRPAIETHEMLYQVIKKHNFLPEAFTTDFRVHWAQHPLR PEFAESTYFLYKATGDPYYLEVGKTLIENLNKYARVPCGFAAMKDVRTGSHEDRMDSFFL AEMFKYLYLLFADKEDIIFDIEDYIFTTEAHLLPLWLSTTNQSISKKNTTSEYTELDDSN FDWTCPNTQILFPNDPLYAQSIREPLKNVVDKSCPRGIIRVEESFRSGAKPPLRARDFMA TNPEHLEILKKMGVSLIHLKDGRVQLVQHAIQAASSIDAEDGLRFMQEMIELSSQQQKEQ QLPPRAVQIVSHPFFGRVVLTAGPAQFGLDLSKHKETRGFVASSKPSNGCSELTNPEAVM GKIALIQRGQCMFAEKARNIQNAGAIGGIVIDDNEGSSSDTAPLFQMAGDGKDTDDIKIP MLFLFSKEGSIILDAIREYEEVEVLLSDKAKDRDPEMENEEQPSSENDSQNQSGEQISSS SQEVDLVDQESSEENSLNSHPESLSLADMDNAASISPSEQTSNPTENHETTNLNGECTDL DNQLQEQSETEEDSNPNVSWGKKVQPIDSILADWNEDIEAFEMMEKDEL*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence
2704
Start on reference sequence
35
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
