Gene:
Gene Symbol:
Cntnap5b
Gene Name:
contactin associated protein-like 5B
Original Clone ID:
FLH481627.01X
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00295380
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 3879nts
Open reading frame: 35 to 3913
GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGGATTCTGTACCAAGACTGACCAG
CATTTTGACTTTGGTGCTGTCTGGCTTGTGGCACGTTGGATTAACAGCGACAAATTACAA
TTGTGATGACCCATTGGCCTCTTTCCTCTCACCGAAGGCTTTCTCCAGTTCCTCAGATCT
CACTGGAAGTTCCAGCTCAGCTCAACTGAATGGGAGAATGGGAACTGGTGGTTGGTCTCC
AGAAAATTCCAGTGCCCATCAGTGGCTGCAGTTGGACCTGGGAAACAGAGTAGAGATTAC
AGCAGTGGCCACACAGGGAAGATATGGGAGCTCTGACTGGGTGACAAGCTATTGCCTGAT
ATTCAGCGACACAGGGCACAACTGGCAACAGTACAAGCAAGGAGACAGCATCTGGACATT
TATAGGAAACATGAATGCTAACAGTGTAGTTCACCACAAGCTACTGCACGCTGTGAGAGC
CCGCTTTGTTCGCTTTGTGCCCTTGGAATGGAATCCAAACGGAAAGATTGGCATGAGAGT
GGAGGTCTATGGATGCTTCTACAAATCAGATGTTGCTGACTTTGATGGCCGGAGCTCACT
TTTGTACAGGTTCAATCAGAAGACAATGAGTACCCTCAAAGATGTGATCTCCCTGAAATT
CAAGAGCATGCAAGGAGATGGGGTTCTGTTCCATGGGGAAGGACAACGTGGAGACCACAT
CACCCTAGAGCTCCAGAATGGAAGACTAGCCCTATACCTGAACTTGGATGATAGCAAAGC
CCGCCTCAGTAGTATTACACCCTCAGCCATTCTGGGTAGCCTTCTGGATGACCAGCACTG
GCACTCAGTTCTCCTGGAGCGTGTGGGAAAGCAAGCAAACTTCACTGTGGACAGGAACAC
CCAGCACTTTCGGACCAACGGTGACACTGATGCCCTGGACATCGACTATGAGCTCAGTTT
TGGAGGAATTCCAGTACCAAGTAAACCTGGGACCTTTTTAAAGAAAAACTTCCAAGGCTG
TATTGAAAACCTTTACTACAATGGAGTGAACATAATTGACTTGGCTAAAAGACGCAAGCA
TCAGATCTACTCCGGCAATGTCACCTTTTCCTGCTCAGAACCACAAATTGTACCTATCAC
ATTTGTCAACTCCAGGAGCAGTTATTTGCTGCTACCTGGCACCCCCCAAATTGATGGTCT
ATCAGTGAGTTTCCAGTTTCGAACATGGAATGAGGATGGTCTGCTTCTGTCCACAGAGCT
GTCTGAAGGCTCAGGAACCCTGCTGCTCATTCTGGAGGGCGGGACTCTGAGACTCCTGAT
TAAGAACGTGGCAGGACATGGAACTGAAAGCCTCACAGGCAGAAGCTTGAATAATGGCCA
GTGGCATTCTGTCAACATCAATGCAAGGAGAAATCGTGTTACTCTCACACTGGATAATGA
TCCTGCTTCTCCAGCTCTGGAAACCCCCTGGCTACAGATATATTCTGGATATAGTTACTA
CTTTGGAGGGTGCCCTGACAATCTCACCGATTCCCAATGCTTAAATCCCATTAAGGCTTT
CCAAGGCTGCATGAGGCTCATCTTTATTGATAACCAGCCCAAGGACCTCATTTCAGTTCA
GCAAGGTTCCCTGGGGAATTTTAGTGATTTACACATTGATCTGTGTAGCATCAAAGACAG
GTGTTTCCCCAACTACTGTGAACATGGAGGACACTGTACTCAAACCTGGACAAACTTCTA
CTGCAACTGCAGTAATACTGGTTACACAGGAGCCACCTGTCATGATTCCATCTATGAGCA
ATCCTGTGAGGTATACAGACACAGAGGAAACACAGCTGGGTACTTCTATGTTGACTCTGA
TGGGAGCGGCCCACTGAGACCACTCCAGGTGTACTGCAATGTTACTGAGGACAAGATCTG
GATGACGGTTCAACACAACAACACAGAGTTGACTTGGATACAGGGTTCCAACACTGAGAA
ACCCTATGTCATGAACTTAAATTATGGTGGGAGTATGGAGCAGCTGGAGGCTCTGATTGA
TGGCTCAGAGCACTGTGAACAGGAGGTAACATACTACTGTAGGAGATCACACCTGCTTAA
CACACCAGATGGAGCCCCATTTGCCTGGTGGACTGGGAGGTCCAATGAGAGGCACCCTTA
CTGGGGAGGTTATTTTCCTGGAGACCAGAAGTGTGGATGTGGCCTAGAGGAGAGCTGCCT
GGAGATGTCAAACGATACTGGTTTTCTTTCCTTCAAAGATCACTTGCCTGTAACTCAGAT
AATTATCAGTGATACCAACAGATCAAACTCAGAAGCAGCTTGGAGAATTGGCCCCTTGCG
TTGCTATGGTGACCGACACTTCTGGAATGCTGTCTCATTTTCTACTGAAGCTGCTTACCT
CCACATTCCTACCTTCCATGCAGAATTCAGCGCTGATATTTCCTTCTTTTTTAAAACCAC
TGCTTTATCTGGCGTTTTCCTAGAAAACCTTGGCGTTAAAAACTTCCTCCGACTTGAAAT
GAGCTCTCCTTCTGAAGTCATATTCGCCATTGATGTTGGGAATGGTCCTATGGAACTCCT
TGTCCAGTCTCCTTATCCTCTGAATGACAACCAATGGCATTACATCCAGGCTGAGAGAAA
CATCAAGGAGACCTTATTACATGTAGACAACCTTCCGAAGAGTATGAGGATGGCCTCAGA
GGAAGGCCATTTTCTGCTTCAGCTGAATAGCCAGTTGTTTGTAGGGGGGACATCATCAAG
ACAGAAAGGCTTTCTTGGATGCATCCGTTCATTACACTTGAATGGGCAGAAAGTAGACCT
GGAAGAGAGGGCAAAGATCACATCTGGAGTCAGACCTGGCTGTGCAGGTCACTGCAACAG
CTATGGAAGGAACTGCCAAAATGGGGGCAAGTGTGTGGAGAAGCATATTGGCTACACCTG
TGACTGTACCAACTCACCATATGAAGGACCTTTCTGCAAGAAAGAGATTTCAGCACTTTT
TTATTCTGGGACCTCTGTTACATACATGTTTCAAGAACCATACCCGGTGACCAAGAACAC
AAGCCTCTCATCTTCAGCTATCTACAAAGATACAGCTCCATCCAAGGAAGCCATCACACT
GAGTTTTATGACAGCACAAGCTCCCACCCTCTTGCTCTACCTCAATTTCTCTTCTCAGAA
TTTTCTGGCTATTCTGCTCTCCAGGAATGGAAGTTTACAGATTCAATATCGACTAAGCAA
GGAAGAAAGTCATGTGTTCACCATCAGCACAGAGAATCTTGCCAATAGAAGAGTACACCA
GGTGAAGATGAGCCGAGATGGGCCAGAACTTTCCATTCAGATGGACCAGCAACTCTTCAG
CTATAACTTCCCCCCAGAAGTGGAGTTCCAAACAGTGAGGTCACTCATCCTAGGCAAAGT
TACAGAAACTCTTGGGTTGGATCCAGAAGTTGCTAAAGCAAATACACTGGGTTTTGTTGG
ATGCCTCTCTTCAGTCCAATATAACCATATCGCACCATTGAAGGCAGCCCTCCGCCATGC
CAGCATTTCTCCTGTGACTGTTCAAGGGACTTTGACAGAGTCCAGCTGTGACTCCATGAT
AGACTCTGATGTGACTGGAGTGACTACTGTTTATTCTCCATCAGTTCCCTTTGGAAAGAC
AGATGAGCATGAACCACTCACAAATACAATCCAGAGTGACTCAGCATTCATTGGAGGTGT
CATAGCAATTGTAACATTTATCACTTTTTGTGTCCTTGGAATCATGGTGCACTTCCTCTA
TCAGCAAAAGCAGTCACACCGTACCAATCAGATGAAGGAGAAGGAATACCCAGACAACTT
GGACAGTTCCTTTAGAAATGACATTGACTTGCAAAACACAGCCATCGAGTGCAAACGGGA
ATACTTCATCTAGGGCCTCATGGGCCCAGCTTTCTTGTAC
Protein sequence predicted based on nucleotide sequence
MDSVPRLTSILTLVLSGLWHVGLTATNYNCDDPLASFLSPKAFSSSSDLTGSSSSAQLNG RMGTGGWSPENSSAHQWLQLDLGNRVEITAVATQGRYGSSDWVTSYCLIFSDTGHNWQQY KQGDSIWTFIGNMNANSVVHHKLLHAVRARFVRFVPLEWNPNGKIGMRVEVYGCFYKSDV ADFDGRSSLLYRFNQKTMSTLKDVISLKFKSMQGDGVLFHGEGQRGDHITLELQNGRLAL YLNLDDSKARLSSITPSAILGSLLDDQHWHSVLLERVGKQANFTVDRNTQHFRTNGDTDA LDIDYELSFGGIPVPSKPGTFLKKNFQGCIENLYYNGVNIIDLAKRRKHQIYSGNVTFSC SEPQIVPITFVNSRSSYLLLPGTPQIDGLSVSFQFRTWNEDGLLLSTELSEGSGTLLLIL EGGTLRLLIKNVAGHGTESLTGRSLNNGQWHSVNINARRNRVTLTLDNDPASPALETPWL QIYSGYSYYFGGCPDNLTDSQCLNPIKAFQGCMRLIFIDNQPKDLISVQQGSLGNFSDLH IDLCSIKDRCFPNYCEHGGHCTQTWTNFYCNCSNTGYTGATCHDSIYEQSCEVYRHRGNT AGYFYVDSDGSGPLRPLQVYCNVTEDKIWMTVQHNNTELTWIQGSNTEKPYVMNLNYGGS MEQLEALIDGSEHCEQEVTYYCRRSHLLNTPDGAPFAWWTGRSNERHPYWGGYFPGDQKC GCGLEESCLEMSNDTGFLSFKDHLPVTQIIISDTNRSNSEAAWRIGPLRCYGDRHFWNAV SFSTEAAYLHIPTFHAEFSADISFFFKTTALSGVFLENLGVKNFLRLEMSSPSEVIFAID VGNGPMELLVQSPYPLNDNQWHYIQAERNIKETLLHVDNLPKSMRMASEEGHFLLQLNSQ LFVGGTSSRQKGFLGCIRSLHLNGQKVDLEERAKITSGVRPGCAGHCNSYGRNCQNGGKC VEKHIGYTCDCTNSPYEGPFCKKEISALFYSGTSVTYMFQEPYPVTKNTSLSSSAIYKDT APSKEAITLSFMTAQAPTLLLYLNFSSQNFLAILLSRNGSLQIQYRLSKEESHVFTISTE NLANRRVHQVKMSRDGPELSIQMDQQLFSYNFPPEVEFQTVRSLILGKVTETLGLDPEVA KANTLGFVGCLSSVQYNHIAPLKAALRHASISPVTVQGTLTESSCDSMIDSDVTGVTTVY SPSVPFGKTDEHEPLTNTIQSDSAFIGGVIAIVTFITFCVLGIMVHFLYQQKQSHRTNQM KEKEYPDNLDSSFRNDIDLQNTAIECKREYFI*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
1
End on reference sequence
3879
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
