Gene:
Gene Symbol:
Itgax
Gene Name:
integrin alpha X
Original Clone ID:
FLH481669.01X
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00295422
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 3510nts
Open reading frame: 35 to 3544
GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGAGCTGTACCTGGATAGCCTTTCT
TCTGCTGTTGGGGTTTGTTTCTTGTCTTGGCTTCAACTTGGATGCAGAGAAGCTGACACA
TTTTCACATGGACGGTGCTGAGTTCGGACACAGTGTGCTCCAGTATGATAGTTCCTGGGT
GGTGGTTGGAGCACCAAAGGAAATAAAAGCCACTAACCAAATAGGTGGCCTCTACAAATG
TGGCTATCACACAGGCAACTGTGAGCCCATCTCCCTCCAGGTGCCCCCAGAGGCTGTGAA
CATATCCCTGGGCCTGTCCCTTGCTGCTGCCACCAACCCTTCCTGGCTGTTGGCTTGTGG
TCCTACTGTGCACCACACATGCAGAGAGAATATATACTTGACAGGGCTCTGCTTTCTACT
GAGTTCATCATTCAAGCAGAGCCAGAACTTCCCAACTGCACAGCAGGAGTGTCCAAAGCA
AGACCAAGACATCGTGTTCCTGATTGATGGCTCGGGTAGCATCAGTTCCACAGATTTTGA
AAAAATGCTGGACTTTGTTAAAGCTGTGATGAGCCAGCTTCAGAGACCTAGCACACGGTT
CTCCCTGATGCAGTTCTCTGATTACTTCCGAGTACATTTTACTTTCAACAACTTCATCTC
CACGTCAAGCCCTTTAAGTCTGTTGGGTTCTGTAAGGCAGCTAAGAGGGTACACATACAC
AGCCTCGGCTATCAAGCATGTCATAACAGAACTGTTCACCACCCAAAGTGGAGCTCGGCA
AGATGCCACCAAGGTCCTCATTGTCATCACTGATGGGAGAAAACAAGGGGACAACTTGAG
TTATGATAGTGTCATCCCCATGGCAGAGGCTGCAAGCATCATTCGTTATGCAATTGGGGT
AGGAAAGGCCTTTTACAATGAACATTCCAAGCAAGAATTAAAGGCCATTGCATCGATGCC
TTCCCATGAATACGTATTCAGCGTGGAGAACTTTGATGCTTTGAAGGATATTGAGAATCA
GCTGAAGGAGAAGATCTTTGCCATTGAGGGCACAGAGACACCAAGCAGCAGTACTTTTGA
ATTGGAGATGTCCCAGGAGGGCTTCAGTGCTGTGTTTACACCTGATGGACCAGTTCTGGG
GGCTGTGGGAAGCTTCAGCTGGTCTGGAGGTGCCTTCTTGTACCCCTCAAATATGAGACC
CACCTTCATCAACATGTCTCAGGAGAACGAGGACATGAGGGACGCTTACCTGGGTTACTC
CACCGCACTGGCCTTTTGGAAGGGGGTCCACAGCCTGATCCTGGGGGCCCCTCGCCACCA
GCACACGGGGAAGGTTGTCATCTTTACCCAGGAATCCAGGCACTGGAGGCCCAAGTCTGA
AGTCAGAGGGACACAGATCGGCTCCTACTTTGGGGCATCTCTTTGTTCTGTGGACATGGA
TAGAGATGGCAGCACTGACCTGGTCCTGATTGGAGTCCCCCATTACTATGAGCACACCCG
AGGGGGGCAGGTGTCAGTGTGCCCCATGCCCGGTGTGGGGAGCAGGTGGCATTGTGGGAC
CACCCTCCATGGGGAGCAGGGCCATCCTTGGGGCCGCTTTGGGGCGGCTCTGACAGTGCT
AGGGGACGTGAATGGGGACAGTCTGGCAGATGTGGCTATTGGTGCACCCGGAGAGGAGGA
GAACAGAGGTGCTGTCTACATATTTCATGGAGCCTCAAGACAGGACATCGCTCCCTCACC
CAGCCAGAGGATTTCAGCATCCCAGATCCCCTCCAGGATTCAATATTTTGGGCAGTCACT
GAGTGGGGGCCAGGACCTCACCCGGGATGGACTGGTGGATCTGGCCGTGGGCTCCAAGGG
ACGCGTGCTGCTGCTCAGGACCAGACCAATTCTTAGAGTGTCACCAACTGTTCACTTCAC
ACCTGCAGAGATTTCCAGGTCTGTGTTTGAGTGTCAGGAGCAGGTGGCCCCTGAGCAGAC
ACTGAGTGATGCCACTGTCTGCCTTCATATTCATGAAAGCCCCAAGACCCAACTAGGTGA
CCTCCGAAGTACTGTCACCTTTGACCTGGCTCTAGACCATGGCCGTCTGAGTACCCGGGC
CATCTTCAAGGAGACAAAGACCCGGGCTCTGACTCGAGTTAAAACTCTTGGTCTGAACAA
GCACTGTGAAAGTGTGAAGTTGCTTCTCCCGGCCTGTGTGGAGGACTCAGTGACCCCGAT
CACTCTTCGCCTCAACTTTTCTCTTGTTGGTGTGCCCATCAGTTCCTTACAAAATCTCCA
ACCCATGCTGGCTGTAGATGACCAAACGTACTTCACGGCCTCTCTTCCCTTTGAGAAGAA
CTGTGGAGCTGACCACATCTGCCAGGATGACCTTAGTGTCGTATTTGGCTTCCCAGACTT
GAAGACCCTGGTGGTGGGAAGTGACCTGGAGCTGAATGTAGATGTGACGGTGTCTAATGA
TGGAGAAGATTCCTACGGAACCACAGTCACTCTGTTCTACCCCGTGGGTCTGTCCTTTAG
ACGAGTTGCAGAAGGCCAAGTATTCCTTCGGAAGAAAGAGGACCAGCAGTGGCAGCGGCG
TGGGCAGCACTCCCTGCACCTCATGTGTGACAGCACCCCAGACAGGAGTCAGGGCCTCTG
GAGTACCAGCTGTAGCAGCAGACATGTCATTTTCCGTGGAGGCTCACAGATGACTTTTTT
GGTAACTTTTGACGTCTCCCCCAAAGCTGAGCTGGGGGACCGGCTGCTTCTAAGAGCCAG
AGTGGGAAGTGAGAATAATGTACCCGGAACCCCAAAAACCACCTTCCAGTTGGAGCTTCC
AGTAAAATATGCGGTGTACACCATGATCAGCAGCCATGACCAGTTTACCAAGTATCTTAA
CTTCTCAACCTCAGAAAAGGAGAAGACCAGTGTGGTCGAACATAGATTCCAGGTGAATAA
CCTGGGCCAGAGAGACGTGCCAGTCAGCATCAACTTTTGGGTGCCCATAGAGCTGAAGGG
AGAAGCTGTGTGGACAGTGATGGTCTCCCACCCTCAGAACCCACTCACCCAATGCTATCG
GAATAGACTCAAGCCAACACAATTTGACCTCCTGACTCACATGCAAAAGAGTCCCGTGCT
GGACTGCTCCATCGCTGACTGCCTGCACCTCCGCTGTGACATCCCCTCCTTGGGCATCCT
GGATGAGCTTTACTTCATTCTGAAGGGCAACCTCAGCTTCGGCTGGATCAGTCAGACATT
GCAGAAAAAGGTGTTGCTCCTGAGTGAGGCTGAAATCACATTCAACACATCTGTGTATTC
CCAGCTGCCGGGACAGGAGGCATTTCTGAGAGCCCAGACGAAGACAGTGCTGGAGATGTA
TAAAGTTCACAACCCCGTCCCTCTTATCGTGGGCAGCTCAGTGGGAGGTCTGCTGCTGCT
GGCTATCATCACAGCTATACTTTACAAGGCTGGTTTCTTCAAACGTCAGTACAAGGAGAT
GTTGGAGGAAGCAAATGGACAGTTTGTCTCAGACGGGACCCCAACTCCCCAGGTTGCCCA
GTAGGGCCTCATGGGCCCAGCTTTCTTGTAC
Protein sequence predicted based on nucleotide sequence
MSCTWIAFLLLLGFVSCLGFNLDAEKLTHFHMDGAEFGHSVLQYDSSWVVVGAPKEIKAT NQIGGLYKCGYHTGNCEPISLQVPPEAVNISLGLSLAAATNPSWLLACGPTVHHTCRENI YLTGLCFLLSSSFKQSQNFPTAQQECPKQDQDIVFLIDGSGSISSTDFEKMLDFVKAVMS QLQRPSTRFSLMQFSDYFRVHFTFNNFISTSSPLSLLGSVRQLRGYTYTASAIKHVITEL FTTQSGARQDATKVLIVITDGRKQGDNLSYDSVIPMAEAASIIRYAIGVGKAFYNEHSKQ ELKAIASMPSHEYVFSVENFDALKDIENQLKEKIFAIEGTETPSSSTFELEMSQEGFSAV FTPDGPVLGAVGSFSWSGGAFLYPSNMRPTFINMSQENEDMRDAYLGYSTALAFWKGVHS LILGAPRHQHTGKVVIFTQESRHWRPKSEVRGTQIGSYFGASLCSVDMDRDGSTDLVLIG VPHYYEHTRGGQVSVCPMPGVGSRWHCGTTLHGEQGHPWGRFGAALTVLGDVNGDSLADV AIGAPGEEENRGAVYIFHGASRQDIAPSPSQRISASQIPSRIQYFGQSLSGGQDLTRDGL VDLAVGSKGRVLLLRTRPILRVSPTVHFTPAEISRSVFECQEQVAPEQTLSDATVCLHIH ESPKTQLGDLRSTVTFDLALDHGRLSTRAIFKETKTRALTRVKTLGLNKHCESVKLLLPA CVEDSVTPITLRLNFSLVGVPISSLQNLQPMLAVDDQTYFTASLPFEKNCGADHICQDDL SVVFGFPDLKTLVVGSDLELNVDVTVSNDGEDSYGTTVTLFYPVGLSFRRVAEGQVFLRK KEDQQWQRRGQHSLHLMCDSTPDRSQGLWSTSCSSRHVIFRGGSQMTFLVTFDVSPKAEL GDRLLLRARVGSENNVPGTPKTTFQLELPVKYAVYTMISSHDQFTKYLNFSTSEKEKTSV VEHRFQVNNLGQRDVPVSINFWVPIELKGEAVWTVMVSHPQNPLTQCYRNRLKPTQFDLL THMQKSPVLDCSIADCLHLRCDIPSLGILDELYFILKGNLSFGWISQTLQKKVLLLSEAE ITFNTSVYSQLPGQEAFLRAQTKTVLEMYKVHNPVPLIVGSSVGGLLLLAIITAILYKAG FFKRQYKEMLEEANGQFVSDGTPTPQVAQ*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence
3562
Start on reference sequence
53
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
