MAD1L1 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  MAD1L1
Gene Name:  MAD1 mitotic arrest deficient-like 1 (yeast)
Original Clone ID:
FLH482039.01L
Keyword:
STOP codon removed based on OC annotation
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format: 
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00295697
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 2154nts
Open reading frame: 1 to 2154

ATGGAAGACCTGGGGGAAAACACCATGGTTTTATCCACCCTGAGATCTTTGAACAACTTC
ATCTCTCAGCGTGTGGAGGGAGGCTCTGGACTGGATATTTCTACCTCGGCCCCAGGTTCT
CTGCAGATGCAGTACCAGCAGAGCATGCAGCTGGAGGAAAGAGCAGAGCAGATCCGTTCG
AAGTCCCACCTCATCCAGGTGGAGCGGGAGAAAATGCAGATGGAGCTGAGTCACAAGAGG
GCTCGAGTGGAGCTGGAGAGAGCAGCCAGCACCAGTGCCAGGAACTACGAGCGTGAGGTC
GACCGCAACCAGGAGCTCCTGACGCGCATCCGGCAGCTTCAGGAGCGGGAGGCCGGGGCG
GAGGAGAAGATGCAGGAGCAGCTGGAGCGCAACAGGCAGTGTCAGCAGAACTTGGATGCT
GCCAGCAAGAGGCTGCGTGAGAAAGAGGACAGTCTGGCCCAGGCTGGCGAGACCATCAAC
GCACTGAAGGGGAGGATCTCGGAACTGCAGTGGAGCGTGATGGACCAGGAGATGCGGGTG
AAGCGCCTGGAGTCGGAGAAGCAGGAGCTGCAGGAGCAGCTGGACCTGCAACACAAAAAA
TGCCAGGAAGCCAATCAGAAAATCCAGGAACTCCAGGCCAGCCAAGAAGCAAGAGCAGAC
CACGAGCAGCAGATTAAGGATCTGGAGCAGAAGCTGTCCCTGCAAGAGCAGGATGCAGCG
ATTGTGAAGAACATGAAGTCTGAGCTGGTACGGCTCCCTAGGCTGGAACGGGAGCTGAAG
CAGCTGCGGGAGGAGAGCGCGCACCTGCGGGAGATGAGAGAGACCAACGGGCTGCTCCAG
GAAGAGCTGGAAGGGCTGCAGAGGAAGCTGGGGCGCCAGGAGAAGATGCAGGAGACGCTG
GTTGGCTTGGAGCTGGAGAACGAGAGGCTGCTGGCCAAGCTGCAAAGCTGGGAGAGACTG
GACCAGACCATGGGCCTGAGCATCAGGACTCCAGAAGACCTTTCCAGATTCGTGGTTGAG
CTGCAGCAGAGGGAGCTTGCCTTGAAGGACAAGAACAGCGCCGTCACCAGCAGCGCCCGG
GGGCTGGAGAAGGCCAGGCAGCAGCTGCAGGAGGAGCTCCGGCAGGTCAGCGGCCAGCTG
TTGGAGGAGAGGAAGAAGCGCGAGACCCACGAGGCGCTGGCCCGGAGGCTCCAGAAACGG
GTCCTGCTGCTCACCAAGGAGCGGGACGGTATGCGGGCCATCCTGGGGTCCTACGACAGC
GAGCTGACCCCGGCCGAGTACTCACCCCAGCTGACGCGGCGCATGCGGGAGGCTGAGGAT
ATGGTGCAGAAGGTGCACAGCCACAGCGCCGAGATGGAGGCTCAGCTGTCGCAGGCCCTG
GAGGAGCTGGGAGGCCAGAAACAAAGAGCAGACATGCTGGAGATGGAGCTGAAGATGCTG
AAGTCTCAGTCCAGCTCTGCCGAACAGAGCTTCCTGTTCTCCAGGGAGGAGGCGGACACG
CTCAGGTTGAAGGTCGAGGAGCTGGAAGGCGAGCGGAGTCGGCTGGAGGAGGAAAAGAGG
ATGCTGGAGGCACAGCTGGAGCGGCGAGCTCTGCAGGGTGACTATGACCAGAGCAGGACC
AAAGTGCTGCACATGAGCCTGAACCCCACCAGTGTGGCCAGGCAGCGCCTGCGCGAGGAC
CACAGCCAGCTGCAGGCGGAGTGCGAGCGACTGCGCGGGCTCCTGCGCGCCATGGAGAGA
GGAGGCACCGTCCCAGCCGACCTTGAGGCTGCCGCCGCGAGTCTGCCATCGTCCAAGGAG
GTGGCAGAGCTGAAGAAGCAGGTGGAGAGTGCCGAGCTGAAGAACCAGCGGCTCAAGGAG
GTTTTCCAGACCAAGATCCAGGAGTTCCGCAAGGCCTGCTACACGCTCACCGGCTACCAG
ATCGACATCACCACGGAGAACCAGTACCGGCTGACCTCGCTGTACGCCGAGCACCCAGGC
GACTGCCTCATCTTCAAGGCCACCAGCCCCTCGGGTTCCAAGATGCAGCTACTGGAGACA
GAGTTCTCACACACCGTGGGCGAGCTCATCGAGGTGCACCTGCGGCGCCAGGACAGCATC
CCTGCCTTCCTCAGCTCGCTCACCCTCGAGCTCTTCAGCCGCCAGACCGTGGCG

Protein sequence predicted based on nucleotide sequence

MEDLGENTMVLSTLRSLNNFISQRVEGGSGLDISTSAPGSLQMQYQQSMQLEERAEQIRS
KSHLIQVEREKMQMELSHKRARVELERAASTSARNYEREVDRNQELLTRIRQLQEREAGA
EEKMQEQLERNRQCQQNLDAASKRLREKEDSLAQAGETINALKGRISELQWSVMDQEMRV
KRLESEKQELQEQLDLQHKKCQEANQKIQELQASQEARADHEQQIKDLEQKLSLQEQDAA
IVKNMKSELVRLPRLERELKQLREESAHLREMRETNGLLQEELEGLQRKLGRQEKMQETL
VGLELENERLLAKLQSWERLDQTMGLSIRTPEDLSRFVVELQQRELALKDKNSAVTSSAR
GLEKARQQLQEELRQVSGQLLEERKKRETHEALARRLQKRVLLLTKERDGMRAILGSYDS
ELTPAEYSPQLTRRMREAEDMVQKVHSHSAEMEAQLSQALEELGGQKQRADMLEMELKML
KSQSSSAEQSFLFSREEADTLRLKVEELEGERSRLEEEKRMLEAQLERRALQGDYDQSRT
KVLHMSLNPTSVARQRLREDHSQLQAECERLRGLLRAMERGGTVPADLEAAAASLPSSKE
VAELKKQVESAELKNQRLKEVFQTKIQEFRKACYTLTGYQIDITTENQYRLTSLYAEHPG
DCLIFKATSPSGSKMQLLETEFSHTVGELIEVHLRRQDSIPAFLSSLTLELFSRQTVA

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 214
End on reference sequence 2370
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
gtacaaaaaagcaggctCCACC
3' Linker Sequence:
TGAATCCacccagctttcttgtaca

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable