DNASU Plasmid Repository • 480.965.5697 | Email

ZDHHC9 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

TOP

Explanation of Terms


Gene: Gene Symbol:  ZDHHC9
Gene Name:  zinc finger, DHHC-type containing 9
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH482269.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  CLOSED
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
HsCD00295927

Price:  Login for Pricing
AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1095nts         Open reading frame : 23 to 1095
Reference Sequence Alignment

GTACAAAAAAGCAGGCTCCACCATGTCTGTGATGGTGGTGAGAAAGAAGGTGACACGGAA
ATGGGAGAAACTCCCAGGCAGGAACACCTTTTGCTGTGATGGCCGCGTCATGATGGCCCG
GCAAAAGGGCATTTTCTACCTGACCCTTTTCCTCATCCTGGGGACATGTACACTCTTCTT
CGCCTTTGAGTGCCGCTACCTGGCTGTTCAGCTGTCTCCTGCCATCCCTGTATTTGCTGC
CATGCTCTTCCTTTTCTCCATGGCTACACTGTTGAGGACCAGCTTCAGTGACCCTGGAGT
GATTCCTCGGGCGCTACCAGATGAAGCAGCTTTCATAGAAATGGAGATAGAAGCTACCAA
TGGTGCGGTGCCCCAGGGCCAGCGACCACCGCCTCGTATCAAGAATTTCCAGATAAACAA
CCAGATTGTGAAACTGAAATACTGTTACACATGCAAGATCTTCCGGCCTCCCCGGGCCTC
CCATTGCAGCATCTGTGACAACTGTGTGGAGCGCTTCGACCATCACTGCCCCTGGGTGGG
GAATTGTGTTGGAAAGAGGAACTACCGCTACTTCTACCTCTTCATCCTTTCTCTCTCCCT
CCTCACAATCTATGTCTTCGCCTTCAACATCGTCTATGTGGCCCTCAAATCTTTGAAAAT
TGGCTTCTTGGAGACATTGAAAGAAACTCCTGGAACTGTTCTAGAAGTCCTCATTTGCTT
CTTTACACTCTGGTCCGTCGTGGGACTGACTGGATTTCATACTTTCCTCGTGGCTCTCAA
CCAGACAACCAATGAAGACATCAAAGGATCATGGACAGGGAAGAATCGCGTCCAGAATCC
CTACAGCCATGGCAATATTGTGAAGAACTGCTGTGAAGTGCTGTGTGGCCCCTTGCCCCC
CAGTGTGCTGGATCGAAGGGGTATTTTGCCACTGGAGGAAAGTGGAAGTCGACCTCCCAG
TACTCAAGAGACCAGTAGCAGCCTCTTGCCACAGAGCCCAGCCCCCACAGAACACCTGAA
CTCAAATGAGATGCCGGAGGACAGCAGCACTCCCGAAGAGATGCCACCTCCAGAGCCCCC
AGAGCCACCACAGGAGGCAGCTGAAGCTGAGAAGTAGCTTGACCCAGCTTTCTTGTAC


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1117
Start on reference sequence 23
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
gtacaaaaaagcaggctCCACC

3' Linker Sequence:
TAGCTTGacccagctttcttgtaca

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ZDHHC9
Symbol Nomenclature : ZDHHC9
SYNONYM : CGI89; CXorf11; DHHC9; MMSA1; MRXSZ; ZDHHC10; ZNF379; ZNF380
Designation : Asp-His-His-Cys domain containing protein 9|antigen MMSA-1|palmitoyltransferase ZDHHC9|zinc finger protein 379|zinc finger protein 380|zinc finger, DHHC domain containing 10
Full Nomenclature : zinc finger, DHHC-type containing 9
GENEID : 51114
Locus Tag : RP6-190D15.1
GI : 190690818
GenBank Accession : EU831584
HGNC : 18475
MIM : 300646
Vega : OTTHUMG00000022375
Target GenBank: EU831584.1

 

Reference Sequence Alignment

  

HsCD00295927        GTACAAAAAAGCAGGCTCCACCATGTCTGTGATGGTGGTGAGAAAGAAGGTGACACGGAA
NM_016032.3         ----------------------ATGTCTGTGATGGTGGTGAGAAAGAAGGTGACACGGAA
NM_001008222.2      ----------------------ATGTCTGTGATGGTGGTGAGAAAGAAGGTGACACGGAA
                                          **************************************

HsCD00295927        ATGGGAGAAACTCCCAGGCAGGAACACCTTTTGCTGTGATGGCCGCGTCATGATGGCCCG
NM_016032.3         ATGGGAGAAACTCCCAGGCAGGAACACCTTTTGCTGTGATGGCCGCGTCATGATGGCCCG
NM_001008222.2      ATGGGAGAAACTCCCAGGCAGGAACACCTTTTGCTGTGATGGCCGCGTCATGATGGCCCG
                    ************************************************************

HsCD00295927        GCAAAAGGGCATTTTCTACCTGACCCTTTTCCTCATCCTGGGGACATGTACACTCTTCTT
NM_016032.3         GCAAAAGGGCATTTTCTACCTGACCCTTTTCCTCATCCTGGGGACATGTACACTCTTCTT
NM_001008222.2      GCAAAAGGGCATTTTCTACCTGACCCTTTTCCTCATCCTGGGGACATGTACACTCTTCTT
                    ************************************************************

HsCD00295927        CGCCTTTGAGTGCCGCTACCTGGCTGTTCAGCTGTCTCCTGCCATCCCTGTATTTGCTGC
NM_016032.3         CGCCTTTGAGTGCCGCTACCTGGCTGTTCAGCTGTCTCCTGCCATCCCTGTATTTGCTGC
NM_001008222.2      CGCCTTTGAGTGCCGCTACCTGGCTGTTCAGCTGTCTCCTGCCATCCCTGTATTTGCTGC
                    ************************************************************

HsCD00295927        CATGCTCTTCCTTTTCTCCATGGCTACACTGTTGAGGACCAGCTTCAGTGACCCTGGAGT
NM_016032.3         CATGCTCTTCCTTTTCTCCATGGCTACACTGTTGAGGACCAGCTTCAGTGACCCTGGAGT
NM_001008222.2      CATGCTCTTCCTTTTCTCCATGGCTACACTGTTGAGGACCAGCTTCAGTGACCCTGGAGT
                    ************************************************************

HsCD00295927        GATTCCTCGGGCGCTACCAGATGAAGCAGCTTTCATAGAAATGGAGATAGAAGCTACCAA
NM_016032.3         GATTCCTCGGGCGCTACCAGATGAAGCAGCTTTCATAGAAATGGAGATAGAAGCTACCAA
NM_001008222.2      GATTCCTCGGGCGCTACCAGATGAAGCAGCTTTCATAGAAATGGAGATAGAAGCTACCAA
                    ************************************************************

HsCD00295927        TGGTGCGGTGCCCCAGGGCCAGCGACCACCGCCTCGTATCAAGAATTTCCAGATAAACAA
NM_016032.3         TGGTGCGGTGCCCCAGGGCCAGCGACCACCGCCTCGTATCAAGAATTTCCAGATAAACAA
NM_001008222.2      TGGTGCGGTGCCCCAGGGCCAGCGACCACCGCCTCGTATCAAGAATTTCCAGATAAACAA
                    ************************************************************

HsCD00295927        CCAGATTGTGAAACTGAAATACTGTTACACATGCAAGATCTTCCGGCCTCCCCGGGCCTC
NM_016032.3         CCAGATTGTGAAACTGAAATACTGTTACACATGCAAGATCTTCCGGCCTCCCCGGGCCTC
NM_001008222.2      CCAGATTGTGAAACTGAAATACTGTTACACATGCAAGATCTTCCGGCCTCCCCGGGCCTC
                    ************************************************************

HsCD00295927        CCATTGCAGCATCTGTGACAACTGTGTGGAGCGCTTCGACCATCACTGCCCCTGGGTGGG
NM_016032.3         CCATTGCAGCATCTGTGACAACTGTGTGGAGCGCTTCGACCATCACTGCCCCTGGGTGGG
NM_001008222.2      CCATTGCAGCATCTGTGACAACTGTGTGGAGCGCTTCGACCATCACTGCCCCTGGGTGGG
                    ************************************************************

HsCD00295927        GAATTGTGTTGGAAAGAGGAACTACCGCTACTTCTACCTCTTCATCCTTTCTCTCTCCCT
NM_016032.3         GAATTGTGTTGGAAAGAGGAACTACCGCTACTTCTACCTCTTCATCCTTTCTCTCTCCCT
NM_001008222.2      GAATTGTGTTGGAAAGAGGAACTACCGCTACTTCTACCTCTTCATCCTTTCTCTCTCCCT
                    ************************************************************

HsCD00295927        CCTCACAATCTATGTCTTCGCCTTCAACATCGTCTATGTGGCCCTCAAATCTTTGAAAAT
NM_016032.3         CCTCACAATCTATGTCTTCGCCTTCAACATCGTCTATGTGGCCCTCAAATCTTTGAAAAT
NM_001008222.2      CCTCACAATCTATGTCTTCGCCTTCAACATCGTCTATGTGGCCCTCAAATCTTTGAAAAT
                    ************************************************************

HsCD00295927        TGGCTTCTTGGAGACATTGAAAGAAACTCCTGGAACTGTTCTAGAAGTCCTCATTTGCTT
NM_016032.3         TGGCTTCTTGGAGACATTGAAAGAAACTCCTGGAACTGTTCTAGAAGTCCTCATTTGCTT
NM_001008222.2      TGGCTTCTTGGAGACATTGAAAGAAACTCCTGGAACTGTTCTAGAAGTCCTCATTTGCTT
                    ************************************************************

HsCD00295927        CTTTACACTCTGGTCCGTCGTGGGACTGACTGGATTTCATACTTTCCTCGTGGCTCTCAA
NM_016032.3         CTTTACACTCTGGTCCGTCGTGGGACTGACTGGATTTCATACTTTCCTCGTGGCTCTCAA
NM_001008222.2      CTTTACACTCTGGTCCGTCGTGGGACTGACTGGATTTCATACTTTCCTCGTGGCTCTCAA
                    ************************************************************

HsCD00295927        CCAGACAACCAATGAAGACATCAAAGGATCATGGACAGGGAAGAATCGCGTCCAGAATCC
NM_016032.3         CCAGACAACCAATGAAGACATCAAAGGATCATGGACAGGGAAGAATCGCGTCCAGAATCC
NM_001008222.2      CCAGACAACCAATGAAGACATCAAAGGATCATGGACAGGGAAGAATCGCGTCCAGAATCC
                    ************************************************************

HsCD00295927        CTACAGCCATGGCAATATTGTGAAGAACTGCTGTGAAGTGCTGTGTGGCCCCTTGCCCCC
NM_016032.3         CTACAGCCATGGCAATATTGTGAAGAACTGCTGTGAAGTGCTGTGTGGCCCCTTGCCCCC
NM_001008222.2      CTACAGCCATGGCAATATTGTGAAGAACTGCTGTGAAGTGCTGTGTGGCCCCTTGCCCCC
                    ************************************************************

HsCD00295927        CAGTGTGCTGGATCGAAGGGGTATTTTGCCACTGGAGGAAAGTGGAAGTCGACCTCCCAG
NM_016032.3         CAGTGTGCTGGATCGAAGGGGTATTTTGCCACTGGAGGAAAGTGGAAGTCGACCTCCCAG
NM_001008222.2      CAGTGTGCTGGATCGAAGGGGTATTTTGCCACTGGAGGAAAGTGGAAGTCGACCTCCCAG
                    ************************************************************

HsCD00295927        TACTCAAGAGACCAGTAGCAGCCTCTTGCCACAGAGCCCAGCCCCCACAGAACACCTGAA
NM_016032.3         TACTCAAGAGACCAGTAGCAGCCTCTTGCCACAGAGCCCAGCCCCCACAGAACACCTGAA
NM_001008222.2      TACTCAAGAGACCAGTAGCAGCCTCTTGCCACAGAGCCCAGCCCCCACAGAACACCTGAA
                    ************************************************************

HsCD00295927        CTCAAATGAGATGCCGGAGGACAGCAGCACTCCCGAAGAGATGCCACCTCCAGAGCCCCC
NM_016032.3         CTCAAATGAGATGCCGGAGGACAGCAGCACTCCCGAAGAGATGCCACCTCCAGAGCCCCC
NM_001008222.2      CTCAAATGAGATGCCGGAGGACAGCAGCACTCCCGAAGAGATGCCACCTCCAGAGCCCCC
                    ************************************************************

HsCD00295927        AGAGCCACCACAGGAGGCAGCTGAAGCTGAGAAGTAGCTTGACCCAGCTTTCTTGTAC
NM_016032.3         AGAGCCACCACAGGAGGCAGCTGAAGCTGAGAAGTAG---------------------
NM_001008222.2      AGAGCCACCACAGGAGGCAGCTGAAGCTGAGAAGTAG---------------------
                    *************************************                     

Protein

UniProt : Q9Y397
HPRD : 06759

Clone

Cloning Information : 482269
HIP Master Clone ID : 399997
IMAGE ID : 100066613
Original Clone ID : FLH482269.01X

Organism


TAX_ID : 9606
Species Specific ID: 51114

Genome

Chromosome : X
Map Location : Xq26.1
Ensembl : ENSG00000188706

Reagents

Labome : DHHC9-antibody