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CA12 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  CA12
Gene Name:  carbonic anhydrase XII
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH482308.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  CLOSED
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / Yes
Publications: None
Authors: The ORFeome Collaboration
HsCD00295966

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1032nts         Open reading frame : 23 to 1032
Reference Sequence Alignment

GTACAAAAAAGCAGGCTCCACCATGCCCCGGCGCAGCATGCACGCGGCGGCCGTGCTCCT
GCTGGTGATCTTAAAGGAACAGCCTTCCAGCCCGGCCCCAGTGAACGGTTCCAAGTGGAC
TTATTTTGGTCCTGATGGGGAGAATAGCTGGTCCAAGAAGTACCCGTCGTGTGGGGGCCT
GCTGCAGTCCCCCATAGACCTGCACAGTGACATCCTCCAGTATGACGCCAGCCTCACGCC
CCTCGAGTTCCAAGGCTACAATCTGTCTGCCAACAAGCAGTTTCTCCTGACCAACAATGG
CCATTCAGTGAAGCTGAACCTGCCCTCGGACATGCACATCCAGGGCCTCCAGTCTCGCTA
CAGTGCCACGCAGCTGCACCTGCACTGGGGGAACCCGAATGACCCGCACGGCTCTGAGCA
CACCGTCAGCGGACAGCACTTCGCCGCCGAGCTGCACATTGTCCATTATAACTCAGACCT
TTATCCTGACGCCAGCACTGCCAGCAACAAGTCAGAAGGCCTCGTTGTCCTGGCTGTTCT
CATTGAGATGGGCTCCTTCAATCCGTCCTATGACAAGATCTTCAGTCACCTTCAACATGT
AAAGTACAAAGGCCAGGAAGCATTCGTCCCGGGATTCAACATTGAAGAGCTGCTTCCGGA
GAGGACCGCTGAATATTACCGCTACCGGGGGTCCCTGACCACACCCCCTTGCAACCCCAC
TGTGCTCTGGACAGTTTTCCGAAACCCCGTGCAAATTTCCCAGGAGCAGCTGCTGGCTTT
GGAGACAGCCCTGTACTGCACACACATGGACGACCCTTCCCCCAGAGAAATGATCAACAA
CTTCCGGCAGGTCCAGAAGTTCGATGAGAGGCTGGTATACACCTCCTTCTCCCAAGGCAT
CATCCTCTCACTGGCCCTGGCTGGCATTCTTGGCATCTGTATTGTGGTGGTGGTGTCCAT
TTGGCTTTTCAGAAGGAAGAGTATCAAAAAAGGTGATAACAAGGGAGTCATTTACAAGCC
AGCCACCAAGATGGAGACTGAGGCCCACGCTTAGCTTGACCCAGCTTTCTTGTAC


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1144
Start on reference sequence 113
Mutations:
None
Discrepancy:
c16a,L6M; c503t,A168V
                                

5' Linker Sequence:
gtacaaaaaagcaggctCCACC

3' Linker Sequence:
TAGCTTGacccagctttcttgtaca

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CA12
Symbol Nomenclature : CA12
SYNONYM : CAXII; HsT18816
Designation : CA-XII|carbonate dehydratase XII|carbonic anhydrase 12|carbonic dehydratase|tumor antigen HOM-RCC-3.1.3
Full Nomenclature : carbonic anhydrase XII
GENEID : 771
GI : 190690896
GenBank Accession : EU831623
HGNC : 1371
MIM : 603263
Target GenBank: BC000278.1

 

Reference Sequence Alignment

  

HsCD00295966      GTACAAAAAAGCAGGCTCCACCATGCCCCGGCGCAGCATGCACGCGGCGGCCGTGCTCCT
NM_001218.3       ----------------------ATGCCCCGGCGCAGCCTGCACGCGGCGGCCGTGCTCCT
NM_206925.1       ----------------------ATGCCCCGGCGCAGCCTGCACGCGGCGGCCGTGCTCCT
                                        ***************.**********************

HsCD00295966      GCTGGTGATCTTAAAGGAACAGCCTTCCAGCCCGGCCCCAGTGAACGGTTCCAAGTGGAC
NM_001218.3       GCTGGTGATCTTAAAGGAACAGCCTTCCAGCCCGGCCCCAGTGAACGGTTCCAAGTGGAC
NM_206925.1       GCTGGTGATCTTAAAGGAACAGCCTTCCAGCCCGGCCCCAGTGAACGGTTCCAAGTGGAC
                  ************************************************************

HsCD00295966      TTATTTTGGTCCTGATGGGGAGAATAGCTGGTCCAAGAAGTACCCGTCGTGTGGGGGCCT
NM_001218.3       TTATTTTGGTCCTGATGGGGAGAATAGCTGGTCCAAGAAGTACCCGTCGTGTGGGGGCCT
NM_206925.1       TTATTTTGGTCCTGATGGGGAGAATAGCTGGTCCAAGAAGTACCCGTCGTGTGGGGGCCT
                  ************************************************************

HsCD00295966      GCTGCAGTCCCCCATAGACCTGCACAGTGACATCCTCCAGTATGACGCCAGCCTCACGCC
NM_001218.3       GCTGCAGTCCCCCATAGACCTGCACAGTGACATCCTCCAGTATGACGCCAGCCTCACGCC
NM_206925.1       GCTGCAGTCCCCCATAGACCTGCACAGTGACATCCTCCAGTATGACGCCAGCCTCACGCC
                  ************************************************************

HsCD00295966      CCTCGAGTTCCAAGGCTACAATCTGTCTGCCAACAAGCAGTTTCTCCTGACCAACAATGG
NM_001218.3       CCTCGAGTTCCAAGGCTACAATCTGTCTGCCAACAAGCAGTTTCTCCTGACCAACAATGG
NM_206925.1       CCTCGAGTTCCAAGGCTACAATCTGTCTGCCAACAAGCAGTTTCTCCTGACCAACAATGG
                  ************************************************************

HsCD00295966      CCATTCAGTGAAGCTGAACCTGCCCTCGGACATGCACATCCAGGGCCTCCAGTCTCGCTA
NM_001218.3       CCATTCAGTGAAGCTGAACCTGCCCTCGGACATGCACATCCAGGGCCTCCAGTCTCGCTA
NM_206925.1       CCATTCAGTGAAGCTGAACCTGCCCTCGGACATGCACATCCAGGGCCTCCAGTCTCGCTA
                  ************************************************************

HsCD00295966      CAGTGCCACGCAGCTGCACCTGCACTGGGGGAACCCGAATGACCCGCACGGCTCTGAGCA
NM_001218.3       CAGTGCCACGCAGCTGCACCTGCACTGGGGGAACCCGAATGACCCGCACGGCTCTGAGCA
NM_206925.1       CAGTGCCACGCAGCTGCACCTGCACTGGGGGAACCCGAATGACCCGCACGGCTCTGAGCA
                  ************************************************************

HsCD00295966      CACCGTCAGCGGACAGCACTTCGCCGCCGAGCTGCACATTGTCCATTATAACTCAGACCT
NM_001218.3       CACCGTCAGCGGACAGCACTTCGCCGCCGAGCTGCACATTGTCCATTATAACTCAGACCT
NM_206925.1       CACCGTCAGCGGACAGCACTTCGCCGCCGAGCTGCACATTGTCCATTATAACTCAGACCT
                  ************************************************************

HsCD00295966      TTATCCTGACGCCAGCACTGCCAGCAACAAGTCAGAAGGCCTCGTTGTCCTGGCTGTTCT
NM_001218.3       TTATCCTGACGCCAGCACTGCCAGCAACAAGTCAGAAGGCCTCGCTGTCCTGGCTGTTCT
NM_206925.1       TTATCCTGACGCCAGCACTGCCAGCAACAAGTCAGAAGGCCTCGCTGTCCTGGCTGTTCT
                  ******************************************** ***************

HsCD00295966      CATTGAGATGGGCTCCTTCAATCCGTCCTATGACAAGATCTTCAGTCACCTTCAACATGT
NM_001218.3       CATTGAGATGGGCTCCTTCAATCCGTCCTATGACAAGATCTTCAGTCACCTTCAACATGT
NM_206925.1       CATTGAGATGGGCTCCTTCAATCCGTCCTATGACAAGATCTTCAGTCACCTTCAACATGT
                  ************************************************************

HsCD00295966      AAAGTACAAAGGCCAGGAAGCATTCGTCCCGGGATTCAACATTGAAGAGCTGCTTCCGGA
NM_001218.3       AAAGTACAAAGGCCAGGAAGCATTCGTCCCGGGATTCAACATTGAAGAGCTGCTTCCGGA
NM_206925.1       AAAGTACAAAGGCCAGGAAGCATTCGTCCCGGGATTCAACATTGAAGAGCTGCTTCCGGA
                  ************************************************************

HsCD00295966      GAGGACCGCTGAATATTACCGCTACCGGGGGTCCCTGACCACACCCCCTTGCAACCCCAC
NM_001218.3       GAGGACCGCTGAATATTACCGCTACCGGGGGTCCCTGACCACACCCCCTTGCAACCCCAC
NM_206925.1       GAGGACCGCTGAATATTACCGCTACCGGGGGTCCCTGACCACACCCCCTTGCAACCCCAC
                  ************************************************************

HsCD00295966      TGTGCTCTGGACAGTTTTCCGAAACCCCGTGCAAATTTCCCAGGAGCAGCTGCTGGCTTT
NM_001218.3       TGTGCTCTGGACAGTTTTCCGAAACCCCGTGCAAATTTCCCAGGAGCAGCTGCTGGCTTT
NM_206925.1       TGTGCTCTGGACAGTTTTCCGAAACCCCGTGCAAATTTCCCAGGAGCAGCTGCTGGCTTT
                  ************************************************************

HsCD00295966      GGAGACAGCCCTGTACTGCACACACATGGACGACCCTTCCCCCAGAGAAATGATCAACAA
NM_001218.3       GGAGACAGCCCTGTACTGCACACACATGGACGACCCTTCCCCCAGAGAAATGATCAACAA
NM_206925.1       GGAGACAGCCCTGTACTGCACACACATGGACGACCCTTCCCCCAGAGAAATGATCAACAA
                  ************************************************************

HsCD00295966      CTTCCGGCAGGTCCAGAAGTTCGATGAGAGGCTGGTATACACCTCCTTCTCCCAAGG---
NM_001218.3       CTTCCGGCAGGTCCAGAAGTTCGATGAGAGGCTGGTATACACCTCCTTCTCCCAAGTGCA
NM_206925.1       CTTCCGGCAGGTCCAGAAGTTCGATGAGAGGCTGGTATACACCTCCTTCTCCCAAGG---
                  ********************************************************    

HsCD00295966      ------------------------------CATCATCCTCTCACTGGCCCTGGCTGGCAT
NM_001218.3       AGTCTGTACTGCGGCAGGACTGAGTCTGGGCATCATCCTCTCACTGGCCCTGGCTGGCAT
NM_206925.1       ------------------------------CATCATCCTCTCACTGGCCCTGGCTGGCAT
                                                ******************************

HsCD00295966      TCTTGGCATCTGTATTGTGGTGGTGGTGTCCATTTGGCTTTTCAGAAGGAAGAGTATCAA
NM_001218.3       TCTTGGCATCTGTATTGTGGTGGTGGTGTCCATTTGGCTTTTCAGAAGGAAGAGTATCAA
NM_206925.1       TCTTGGCATCTGTATTGTGGTGGTGGTGTCCATTTGGCTTTTCAGAAGGAAGAGTATCAA
                  ************************************************************

HsCD00295966      AAAAGGTGATAACAAGGGAGTCATTTACAAGCCAGCCACCAAGATGGAGACTGAGGCCCA
NM_001218.3       AAAAGGTGATAACAAGGGAGTCATTTACAAGCCAGCCACCAAGATGGAGACTGAGGCCCA
NM_206925.1       AAAAGGTGATAACAAGGGAGTCATTTACAAGCCAGCCACCAAGATGGAGACTGAGGCCCA
                  ************************************************************

HsCD00295966      CGCTTAGCTTGACCCAGCTTTCTTGTAC
NM_001218.3       CGCTTGA---------------------
NM_206925.1       CGCTTGA---------------------
                  *****..                     

Pathways

Reactome : Metabolism
Reactome : Reversible hydration of carbon dioxide
WikiPathway : Epithelium TarBase
WikiPathway : Leukocyte TarBase
WikiPathway : Muscle cell TarBase

Protein

SMART domain : Carb_anhydrase : Eukaryotic-type carbonic anhydrase
UniProt : O43570
HPRD : 04464

Clone

Cloning Information : 482308
HIP Master Clone ID : 400036
IMAGE ID : 100066652
Original Clone ID : FLH482308.01X

Organism


TAX_ID : 9606
Species Specific ID: 771

Genome

Chromosome : 15
Map Location : 15q22

Reagents

Labome : CA12-antibody